4yuf - Revision history

OCA at 07:26, 10 May 2023

2023-05-10T07:26:16Z

←Older revision		Revision as of 07:26, 10 May 2023
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	<StructureSection load='4yuf' size='340' side='right'caption='[[4yuf]], [[Resolution\|resolution]] 1.54Å' scene=''>		<StructureSection load='4yuf' size='340' side='right'caption='[[4yuf]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4yuf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YUF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4YUF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4yuf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corallococcus_coralloides Corallococcus coralloides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YUF FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4yuc\|4yuc]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yuf OCA], [https://pdbe.org/4yuf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yuf RCSB], [https://www.ebi.ac.uk/pdbsum/4yuf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yuf ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4yuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yuf OCA], [~~http~~://pdbe.org/4yuf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4yuf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4yuf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4yuf ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D7RK32_CORCK D7RK32_CORCK]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Corallococcus coralloides]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Stehle, T]]	+	[[Category: Stehle T]]
-	[[Category: Vilstrup, J]]	+	[[Category: Vilstrup J]]
-	[[Category: Zocher, G]]	+	[[Category: Zocher G]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Interconnecting ketosynthase]]~~	+
-	~~[[Category: Thiolase superfamily~~]]	+

OCA at 07:41, 4 March 2020

2020-03-04T07:41:08Z

←Older revision		Revision as of 07:41, 4 March 2020
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	==Crystal Structure of CorB==		==Crystal Structure of CorB==
-	<StructureSection load='4yuf' size='340' side='right' caption='[[4yuf]], [[Resolution\|resolution]] 1.54Å' scene=''>	+	<StructureSection load='4yuf' size='340' side='right'caption='[[4yuf]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4yuf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YUF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YUF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4yuf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YUF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YUF FirstGlance]. <br>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]
	[[Category: Vilstrup, J]]		[[Category: Vilstrup, J]]

OCA at 11:03, 24 August 2017

2017-08-24T11:03:57Z

←Older revision		Revision as of 11:03, 24 August 2017
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	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4yuc\|4yuc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4yuc\|4yuc]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yuf OCA], [http://pdbe.org/4yuf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yuf RCSB], [http://www.ebi.ac.uk/pdbsum/4yuf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yuf OCA], [http://pdbe.org/4yuf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yuf RCSB], [http://www.ebi.ac.uk/pdbsum/4yuf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yuf ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Corallopyronin A is a polyketide derived from the myxobacterium Corallococcus coralloides with potent antibiotic features. The gene cluster responsible for the biosynthesis of corallopyronin A has been described recently, and it was proposed that CorB acts as a ketosynthase to interconnect two polyketide chains in a rare head-to-head condensation reaction. We determined the structure of CorB, the interconnecting polyketide synthase, to high resolution and found that CorB displays a thiolase fold. Site-directed mutagenesis showed that the catalytic triad consisting of a cysteine, a histidine and an asparagine is crucial for catalysis, and that this triad shares similarities with the triad found in HMG-CoA synthases. We synthesized a substrate mimic to derivatize purified CorB and confirmed substrate attachment by ESI-MS. Structural analysis of the complex yielded an electron density-based model for the polyketide chain and showed that the unusually wide, T-shaped active site is able to accommodate two polyketides simultaneously. Our structural analysis provides a platform for understanding the unusual head-to-head polyketide-interconnecting reaction catalyzed by CorB.
		+
		+	Structural basis of head to head polyketide fusion by CorB.,Zocher G, Vilstrup J, Heine D, Hallab A, Goralski E, Hertweck C, Stahl M, Schaberle TF, Stehle T Chem Sci. 2015 Nov 13;6(11):6525-6536. doi: 10.1039/c5sc02488a. Epub 2015 Aug 6. PMID:28757960<ref>PMID:28757960</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4yuf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 20:26, 11 May 2016

2016-05-11T20:26:22Z

←Older revision		Revision as of 20:26, 11 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 4yuf is ~~ON HOLD~~	+	==Crystal Structure of CorB==
-		+	<StructureSection load='4yuf' size='340' side='right' caption='[[4yuf]], [[Resolution\|resolution]] 1.54Å' scene=''>
-	~~Authors~~: ~~Zocher~~, G., ~~Vilstrup~~, J., ~~Stehle, T~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4yuf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YUF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YUF FirstGlance]. <br>
-	~~Description:~~	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4yuc\|4yuc]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yuf OCA], [http://pdbe.org/4yuf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yuf RCSB], [http://www.ebi.ac.uk/pdbsum/4yuf PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Stehle, T]]
	[[Category: Vilstrup, J]]		[[Category: Vilstrup, J]]
	[[Category: Zocher, G]]		[[Category: Zocher, G]]
-	[[Category: ~~Stehle, T~~]]	+	[[Category: Hydrolase]]
		+	[[Category: Interconnecting ketosynthase]]
		+	[[Category: Thiolase superfamily]]

OCA at 23:44, 30 November 2015

2015-11-30T23:44:48Z

←Older revision		Revision as of 23:44, 30 November 2015
Line 7:		Line 7:
	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Vilstrup, J]]
	[[Category: Zocher, G]]		[[Category: Zocher, G]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]
-	[[Category: Vilstrup, J]]

OCA: Protected "4yuf" [edit=sysop:move=sysop]

2015-03-26T12:51:51Z

Protected "4yuf" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:51, 26 March 2015

OCA: New page: '''Unreleased structure''' The entry 4yuf is ON HOLD Authors: Zocher, G., Vilstrup, J., Stehle, T. Description: Category: Unreleased Structures Category: Zocher, G [[Category:...

2015-03-26T12:51:50Z

New page: '''Unreleased structure''' The entry 4yuf is ON HOLD Authors: Zocher, G., Vilstrup, J., Stehle, T. Description: Category: Unreleased Structures Category: Zocher, G [[Category:...

New page

'''Unreleased structure'''

The entry 4yuf is ON HOLD

Authors: Zocher, G., Vilstrup, J., Stehle, T.

Description:
[[Category: Unreleased Structures]]
[[Category: Zocher, G]]
[[Category: Stehle, T]]
[[Category: Vilstrup, J]]