4z5v - Revision history

OCA at 07:40, 10 May 2023

2023-05-10T07:40:18Z

←Older revision		Revision as of 07:40, 10 May 2023
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	<StructureSection load='4z5v' size='340' side='right'caption='[[4z5v]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='4z5v' size='340' side='right'caption='[[4z5v]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4z5v]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cvma5 Cvma5~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z5V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4Z5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4z5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_hepatitis_virus_strain_A59 Murine hepatitis virus strain A59]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z5V FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">2a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11142 CVMA5])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z5v OCA], [https://pdbe.org/4z5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z5v RCSB], [https://www.ebi.ac.uk/pdbsum/4z5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z5v ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4z5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z5v OCA], [~~http~~://pdbe.org/4z5v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4z5v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4z5v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4z5v ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NS2A_CVMA5 NS2A_CVMA5]] Not essential for virus replication in transformed murine cells.	+	[https://www.uniprot.org/uniprot/NS2A_CVMA5 NS2A_CVMA5] Not essential for virus replication in transformed murine cells.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cvma5]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Huang, J H~~]]	+	[[Category: Murine hepatitis virus strain A59]]
-	[[Category: ~~Peng, G Q~~]]	+	[[Category: Huang JH]]
-	[[Category: ~~Sui, B K~~]]	+	[[Category: Peng GQ]]
-	[[Category: ~~Zhao, L~~]]	+	[[Category: Sui BK]]
-	[[Category: ~~Murine hepatitis virus]]~~	+	[[Category: Zhao L]]
-	~~[[Category: Ns2 protein]]~~	+
-	~~[[Category: Phosphodiesterase]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 06:56, 19 June 2019

2019-06-19T06:56:23Z

←Older revision		Revision as of 06:56, 19 June 2019
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	==Crystal Structure of MHV ns2 PDE Domain==		==Crystal Structure of MHV ns2 PDE Domain==
-	<StructureSection load='4z5v' size='340' side='right' caption='[[4z5v]], [[Resolution\|resolution]] 3.05Å' scene=''>	+	<StructureSection load='4z5v' size='340' side='right'caption='[[4z5v]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4z5v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4z5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvma5 Cvma5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z5V FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z5v OCA], [http://pdbe.org/4z5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z5v RCSB], [http://www.ebi.ac.uk/pdbsum/4z5v PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">2a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11142 CVMA5])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z5v OCA], [http://pdbe.org/4z5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z5v RCSB], [http://www.ebi.ac.uk/pdbsum/4z5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z5v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cvma5]]
		+	[[Category: Large Structures]]
	[[Category: Huang, J H]]		[[Category: Huang, J H]]
	[[Category: Peng, G Q]]		[[Category: Peng, G Q]]

OCA at 16:16, 10 May 2016

2016-05-10T16:16:15Z

←Older revision		Revision as of 16:16, 10 May 2016
Line 1:		Line 1:
		+
	==Crystal Structure of MHV ns2 PDE Domain==		==Crystal Structure of MHV ns2 PDE Domain==
	<StructureSection load='4z5v' size='340' side='right' caption='[[4z5v]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='4z5v' size='340' side='right' caption='[[4z5v]], [[Resolution\|resolution]] 3.05Å' scene=''>

OCA at 17:16, 10 February 2016

2016-02-10T17:16:44Z

←Older revision		Revision as of 17:16, 10 February 2016
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-	'''~~Unreleased~~ structure'''	+	==Crystal Structure of MHV ns2 PDE Domain==
		+	<StructureSection load='4z5v' size='340' side='right' caption='[[4z5v]], [[Resolution\|resolution]] 3.05Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4z5v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z5V FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z5v OCA], [http://pdbe.org/4z5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z5v RCSB], [http://www.ebi.ac.uk/pdbsum/4z5v PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NS2A_CVMA5 NS2A_CVMA5]] Not essential for virus replication in transformed murine cells.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Prior studies have demonstrated that the mouse hepatitis virus A59 strain (MHV-A59) ns2 protein is a member of the 2H phosphoesterase family and exhibits 2',5'-phosphodiesterase (2',5'-PDE) activity. During the interferon antiviral response, ns2 cleaves 2',5'-oligoadenylate (2-5A), a key mediator of RNase L activation, thereby subverting the activation of RNase L and evading host innate immunity. However, the mechanism of 2-5A cleavage by ns2 remains unclear. Here, we present the crystal structure of the MHV ns2 PDE domain and demonstrate a PDE fold similar to that of the cellular protein, a kinase anchoring protein 7 central domain (AKAP7CD) and rotavirus VP3 carboxy-terminal domain (VP3-CTD). The structure displays a pair of strictly conserved HxS/Tx motifs and forms a deep positively charged catalytic groove with beta-sheets and an arginine containing loop (R-loop). These findings provide insight into the structural basis for 2-5A binding of MHV ns2.

-	~~The entry 4z5v is ON HOLD until Paper Publication~~	+	Crystal structure of the mouse hepatitis virus ns2 phosphodiesterase domain that antagonizes RNase L activation.,Sui B, Huang J, Jha BK, Yin P, Zhou M, Fu ZF, Silverman RH, Weiss SR, Peng G, Zhao L J Gen Virol. 2016 Jan 12. doi: 10.1099/jgv.0.000395. PMID:26757803<ref>PMID:26757803</ref>

-	~~Authors: Sui~~, B.K.~~, Huang, J.H., Peng, G.Q., Zhao, L~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal Structure of MHV ns2 PDE Domain~~	+	<div class="pdbe-citations 4z5v" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
-	[[Category: Peng, G.Q]]	+	<references/>
-	[[Category: Sui, B.K]]	+	__TOC__
-	~~[[Category: Huang, J.H~~]]	+	</StructureSection>
		+	[[Category: Huang, J H]]
		+	[[Category: Peng, G Q]]
		+	[[Category: Sui, B K]]
	[[Category: Zhao, L]]		[[Category: Zhao, L]]
		+	[[Category: Murine hepatitis virus]]
		+	[[Category: Ns2 protein]]
		+	[[Category: Phosphodiesterase]]
		+	[[Category: Viral protein]]

OCA at 09:46, 7 October 2015

2015-10-07T09:46:50Z

←Older revision		Revision as of 09:46, 7 October 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4z5v is ON HOLD	+	The entry 4z5v is ON HOLD until Paper Publication

	Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.		Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.

OCA at 10:20, 30 September 2015

2015-09-30T10:20:15Z

←Older revision		Revision as of 10:20, 30 September 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4z5v is ON HOLD ~~until Paper Publication~~	+	The entry 4z5v is ON HOLD

	Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.		Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.

OCA at 07:29, 9 September 2015

2015-09-09T07:29:43Z

←Older revision		Revision as of 07:29, 9 September 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4z5v is ON HOLD	+	The entry 4z5v is ON HOLD until Paper Publication

	Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.		Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.

OCA at 08:59, 2 September 2015

2015-09-02T08:59:15Z

←Older revision		Revision as of 08:59, 2 September 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4z5v is ON HOLD ~~until sometime in the future~~	+	The entry 4z5v is ON HOLD

	Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.		Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.

OCA at 10:43, 15 July 2015

2015-07-15T10:43:26Z

←Older revision		Revision as of 10:43, 15 July 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4z5v is ON HOLD	+	The entry 4z5v is ON HOLD until sometime in the future

	Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.		Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.

OCA at 12:12, 8 July 2015

2015-07-08T12:12:58Z

←Older revision		Revision as of 12:12, 8 July 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4z5v is ON HOLD ~~until Paper Publication~~	+	The entry 4z5v is ON HOLD

	Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.		Authors: Sui, B.K., Huang, J.H., Peng, G.Q., Zhao, L.