4z85 - Revision history

OCA at 15:42, 8 November 2023

2023-11-08T15:42:32Z

←Older revision		Revision as of 15:42, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4z85]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z85 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4z85]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z85 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z85 OCA], [https://pdbe.org/4z85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z85 RCSB], [https://www.ebi.ac.uk/pdbsum/4z85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z85 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z85 OCA], [https://pdbe.org/4z85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z85 RCSB], [https://www.ebi.ac.uk/pdbsum/4z85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z85 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	==See Also==		==See Also==
-	*[[Nitroreductase\|Nitroreductase]]	+	*[[Nitroreductase 3D structures\|Nitroreductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:42, 10 May 2023

2023-05-10T07:42:52Z

←Older revision		Revision as of 07:42, 10 May 2023
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	<StructureSection load='4z85' size='340' side='right'caption='[[4z85]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4z85' size='340' side='right'caption='[[4z85]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4z85]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus~~ fluorescens ~~liquefaciens" flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z85 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4Z85 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4z85]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z85 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nbaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 "Bacillus fluorescens liquefaciens" Flugge 1886])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z85 OCA], [https://pdbe.org/4z85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z85 RCSB], [https://www.ebi.ac.uk/pdbsum/4z85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z85 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4z85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z85 OCA], [~~http~~://pdbe.org/4z85 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4z85 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4z85 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4z85 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A4UVY1_PSEFL A4UVY1_PSEFL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus fluorescens liquefaciens flugge 1886]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ha, N C~~]]	+	[[Category: Pseudomonas fluorescens]]
-	[[Category: ~~Jiao, L~~]]	+	[[Category: Ha NC]]
-	[[Category: ~~Kim, J S~~]]	+	[[Category: Jiao L]]
-	[[Category: ~~2-nitrobenzoate methyl ester]]~~	+	[[Category: Kim JS]]
-	~~[[Category: Cysteine reactivity]]~~	+
-	~~[[Category: Fmn-binding site]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Tyrosine modification~~]]	+

OCA at 07:24, 19 June 2019

2019-06-19T07:24:21Z

←Older revision		Revision as of 07:24, 19 June 2019
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		+
	==Crystal structur of Pseudomonas fluorescens 2-nitrobenzoate 2-nitroreductase NbaA==		==Crystal structur of Pseudomonas fluorescens 2-nitrobenzoate 2-nitroreductase NbaA==
-	<StructureSection load='4z85' size='340' side='right' caption='[[4z85]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='4z85' size='340' side='right'caption='[[4z85]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4z85]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z85 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z85 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4z85]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus fluorescens liquefaciens" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z85 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z85 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z85 OCA], [http://pdbe.org/4z85 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z85 RCSB], [http://www.ebi.ac.uk/pdbsum/4z85 PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nbaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 "Bacillus fluorescens liquefaciens" Flugge 1886])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z85 OCA], [http://pdbe.org/4z85 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z85 RCSB], [http://www.ebi.ac.uk/pdbsum/4z85 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z85 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 4z85" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4z85" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nitroreductase\|Nitroreductase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus fluorescens liquefaciens flugge 1886]]
		+	[[Category: Large Structures]]
	[[Category: Ha, N C]]		[[Category: Ha, N C]]
	[[Category: Jiao, L]]		[[Category: Jiao, L]]

OCA at 17:04, 20 January 2016

2016-01-20T17:04:53Z

←Older revision		Revision as of 17:04, 20 January 2016
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-	'''~~Unreleased~~ structure'''	+	==Crystal structur of Pseudomonas fluorescens 2-nitrobenzoate 2-nitroreductase NbaA==
		+	<StructureSection load='4z85' size='340' side='right' caption='[[4z85]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4z85]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z85 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z85 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z85 OCA], [http://pdbe.org/4z85 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z85 RCSB], [http://www.ebi.ac.uk/pdbsum/4z85 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacterial 2-nitroreductase NbaA is the primary enzyme initiating the degradation of 2-nitrobenzoate (2-NBA), and its activity is controlled by posttranslational modifications. To date, the structure of NbaA remains to be elucidated. In this study, the crystal structure of a Cys194Ala NbaA mutant was determined to a 1.7-A resolution. The substrate analog 2-NBA methyl ester was used to decipher the substrate binding site by inhibition of the wild-type NbaA protein. Tandem mass spectrometry showed that 2-NBA methyl ester produced a 2-NBA ester bond at the Tyr193 residue in the wild-type NbaA but not residues in the Tyr193Phe mutant. Moreover, covalent binding of the 2-NBA methyl ester to Tyr193 reduced the reactivity of the Cys194 residue on the peptide link. The Tyr193 hydroxyl group was shown to be essential for enzyme catalysis, as a Tyr193Phe mutant resulted in fast dissociation of flavin mononucleotide (FMN) from the protein with the reduced reactivity of Cys194. FMN binding to NbaA varied with solution NaCl concentration, which was related to the catalytic activity but not to cysteine reactivity. These observations suggest that the Cys194 reactivity is negatively affected by a posttranslational modification of the adjacent Tyr193 residue, which interacts with FMN and the substrate in the NbaA catalytic site.

-	~~The entry 4z85 is ON HOLD until Paper Publication~~	+	Structural and Mechanistic Insights into the Pseudomonas fluorescens 2-Nitrobenzoate 2-Nitroreductase NbaA.,Kim YH, Song W, Kim JS, Jiao L, Lee K, Ha NC Appl Environ Microbiol. 2015 Aug;81(15):5266-77. doi: 10.1128/AEM.01289-15. Epub , 2015 May 29. PMID:26025888<ref>PMID:26025888</ref>

-	~~Authors: Ha~~, N.C.~~, Jiao, L., Kim, J.S~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structur of Pseudomonas fluorescens 2~~-~~nitrobenzoate 2~~-~~nitroreductase NbaA~~	+	<div class="pdbe-citations 4z85" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ha, N C]]
	[[Category: Jiao, L]]		[[Category: Jiao, L]]
-	[[Category: Ha, ~~N.C~~]]	+	[[Category: Kim, J S]]
-	[[Category: ~~Kim, J.S~~]]	+	[[Category: 2-nitrobenzoate methyl ester]]
		+	[[Category: Cysteine reactivity]]
		+	[[Category: Fmn-binding site]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Tyrosine modification]]

OCA at 23:55, 30 November 2015

2015-11-30T23:55:16Z

←Older revision		Revision as of 23:55, 30 November 2015
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Jiao, L]]		[[Category: Jiao, L]]
-	[[Category: Kim, J.S]]
	[[Category: Ha, N.C]]		[[Category: Ha, N.C]]
		+	[[Category: Kim, J.S]]

OCA at 10:03, 30 April 2015

2015-04-30T10:03:57Z

←Older revision		Revision as of 10:03, 30 April 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4z85 is ON HOLD	+	The entry 4z85 is ON HOLD until Paper Publication

	Authors: Ha, N.C., Jiao, L., Kim, J.S.		Authors: Ha, N.C., Jiao, L., Kim, J.S.

OCA: Protected "4z85" [edit=sysop:move=sysop]

2015-04-15T11:47:27Z

Protected "4z85" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:47, 15 April 2015

OCA: New page: '''Unreleased structure''' The entry 4z85 is ON HOLD Authors: Ha, N.C., Jiao, L., Kim, J.S. Description: Crystal structur of Pseudomonas fluorescens 2-nitrobenzoate 2-nitroreductase Nb...

2015-04-15T11:47:26Z

New page: '''Unreleased structure''' The entry 4z85 is ON HOLD Authors: Ha, N.C., Jiao, L., Kim, J.S. Description: Crystal structur of Pseudomonas fluorescens 2-nitrobenzoate 2-nitroreductase Nb...

New page

'''Unreleased structure'''

The entry 4z85 is ON HOLD

Authors: Ha, N.C., Jiao, L., Kim, J.S.

Description: Crystal structur of Pseudomonas fluorescens 2-nitrobenzoate 2-nitroreductase NbaA
[[Category: Unreleased Structures]]
[[Category: Jiao, L]]
[[Category: Kim, J.S]]
[[Category: Ha, N.C]]