4zi5 - Revision history

OCA at 11:00, 10 January 2024

2024-01-10T11:00:15Z

←Older revision		Revision as of 11:00, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4zi5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenome Metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZI5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4zi5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenome Metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZI5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.702Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zi5 OCA], [https://pdbe.org/4zi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zi5 RCSB], [https://www.ebi.ac.uk/pdbsum/4zi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zi5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zi5 OCA], [https://pdbe.org/4zi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zi5 RCSB], [https://www.ebi.ac.uk/pdbsum/4zi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zi5 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:28, 18 May 2023

2023-05-18T07:28:51Z

←Older revision		Revision as of 07:28, 18 May 2023
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	<StructureSection load='4zi5' size='340' side='right'caption='[[4zi5]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4zi5' size='340' side='right'caption='[[4zi5]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zi5]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Environmental_sequence Environmental sequence~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZI5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ZI5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zi5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenome Metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZI5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryldialkylphosphatase Aryldialkylphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.1 3.1.8.1] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zi5 OCA], [https://pdbe.org/4zi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zi5 RCSB], [https://www.ebi.ac.uk/pdbsum/4zi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zi5 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4zi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zi5 OCA], [~~http~~://pdbe.org/4zi5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4zi5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4zi5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4zi5 ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aryldialkylphosphatase]]
-	[[Category: Environmental sequence]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Colin, P Y]]	+	[[Category: Metagenome]]
-	[[Category: Fischer, G]]	+	[[Category: Colin P-Y]]
-	[[Category: Hollfelder, F]]	+	[[Category: Fischer G]]
-	[[Category: Hyvonen, M]]	+	[[Category: Hollfelder F]]
-	~~[[Category: Alpha/beta hydrolase]]~~	+	[[Category: Hyvonen M]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Metagenomic library]]~~	+
-	~~[[Category: Phosphotriesterase]]~~	+
-	~~[[Category: Promiscuity~~]]	+

OCA at 07:02, 19 June 2019

2019-06-19T07:02:18Z

←Older revision		Revision as of 07:02, 19 June 2019
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	==Crystal Structure of Dienelactone Hydrolase-like Promiscuous Phospotriesterase P91 from Metagenomic Libraries==		==Crystal Structure of Dienelactone Hydrolase-like Promiscuous Phospotriesterase P91 from Metagenomic Libraries==
-	<StructureSection load='4zi5' size='340' side='right' caption='[[4zi5]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='4zi5' size='340' side='right'caption='[[4zi5]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4zi5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZI5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZI5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4zi5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Environmental_sequence Environmental sequence]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZI5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZI5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryldialkylphosphatase Aryldialkylphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.1 3.1.8.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryldialkylphosphatase Aryldialkylphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.1 3.1.8.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zi5 OCA], [http://pdbe.org/4zi5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zi5 RCSB], [http://www.ebi.ac.uk/pdbsum/4zi5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zi5 OCA], [http://pdbe.org/4zi5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zi5 RCSB], [http://www.ebi.ac.uk/pdbsum/4zi5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zi5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Aryldialkylphosphatase]]		[[Category: Aryldialkylphosphatase]]
		+	[[Category: Environmental sequence]]
		+	[[Category: Large Structures]]
	[[Category: Colin, P Y]]		[[Category: Colin, P Y]]
	[[Category: Fischer, G]]		[[Category: Fischer, G]]

OCA at 02:39, 21 February 2016

2016-02-21T02:39:58Z

←Older revision		Revision as of 02:39, 21 February 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 4zi5 is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of Dienelactone Hydrolase-like Promiscuous Phospotriesterase P91 from Metagenomic Libraries==
		+	<StructureSection load='4zi5' size='340' side='right' caption='[[4zi5]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4zi5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZI5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZI5 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryldialkylphosphatase Aryldialkylphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.1 3.1.8.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zi5 OCA], [http://pdbe.org/4zi5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zi5 RCSB], [http://www.ebi.ac.uk/pdbsum/4zi5 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Unculturable bacterial communities provide a rich source of biocatalysts, but their experimental discovery by functional metagenomics is difficult, because the odds are stacked against the experimentor. Here we demonstrate functional screening of a million-membered metagenomic library in microfluidic picolitre droplet compartments. Using bait substrates, new hydrolases for sulfate monoesters and phosphotriesters were identified, mostly based on promiscuous activities presumed not to be under selection pressure. Spanning three protein superfamilies, these break new ground in sequence space: promiscuity now connects enzymes with only distantly related sequences. Most hits could not have been predicted by sequence analysis, because the desired activities have never been ascribed to similar sequences, showing how this approach complements bioinformatic harvesting of metagenomic sequencing data. Functional screening of a library of unprecedented size with excellent assay sensitivity has been instrumental in identifying rare genes constituting catalytically versatile hubs in sequence space as potential starting points for the acquisition of new functions.

-	~~Authors: Colin, P.~~-Y., ~~Fischer~~, G., ~~Hyvonen~~, M., ~~Hollfelder~~, F.	+	Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics.,Colin PY, Kintses B, Gielen F, Miton CM, Fischer G, Mohamed MF, Hyvonen M, Morgavi DP, Janssen DB, Hollfelder F Nat Commun. 2015 Dec 7;6:10008. doi: 10.1038/ncomms10008. PMID:26639611<ref>PMID:26639611</ref>

-	~~Description: Crystal Structure~~ of ~~Dienelactone Hydrolase-like Promiscuous Phospotriesterase P91 from Metagenomic Libraries~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Hyvonen, M~~]]	+	<div class="pdbe-citations 4zi5" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Hollfelder~~, F]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aryldialkylphosphatase]]
		+	[[Category: Colin, P Y]]
	[[Category: Fischer, G]]		[[Category: Fischer, G]]
-	[[Category: ~~Colin~~, ~~P.-Y~~]]	+	[[Category: Hollfelder, F]]
		+	[[Category: Hyvonen, M]]
		+	[[Category: Alpha/beta hydrolase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Metagenomic library]]
		+	[[Category: Phosphotriesterase]]
		+	[[Category: Promiscuity]]

OCA at 00:02, 1 December 2015

2015-12-01T00:02:16Z

←Older revision		Revision as of 00:02, 1 December 2015
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	[[Category: Hyvonen, M]]		[[Category: Hyvonen, M]]
	[[Category: Hollfelder, F]]		[[Category: Hollfelder, F]]
-	[[Category: Colin, P.-Y]]
	[[Category: Fischer, G]]		[[Category: Fischer, G]]
		+	[[Category: Colin, P.-Y]]

OCA at 10:59, 13 May 2015

2015-05-13T10:59:50Z

←Older revision		Revision as of 10:59, 13 May 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4zi5 is ON HOLD	+	The entry 4zi5 is ON HOLD until Paper Publication

	Authors: Colin, P.-Y., Fischer, G., Hyvonen, M., Hollfelder, F.		Authors: Colin, P.-Y., Fischer, G., Hyvonen, M., Hollfelder, F.

OCA: Protected "4zi5" [edit=sysop:move=sysop]

2015-05-06T11:45:37Z

Protected "4zi5" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:45, 6 May 2015

OCA: New page: '''Unreleased structure''' The entry 4zi5 is ON HOLD Authors: Colin, P.-Y., Fischer, G., Hyvonen, M., Hollfelder, F. Description: Crystal Structure of Dienelactone Hydrolase-like Promi...

2015-05-06T11:45:36Z

New page: '''Unreleased structure''' The entry 4zi5 is ON HOLD Authors: Colin, P.-Y., Fischer, G., Hyvonen, M., Hollfelder, F. Description: Crystal Structure of Dienelactone Hydrolase-like Promi...

New page

'''Unreleased structure'''

The entry 4zi5 is ON HOLD

Authors: Colin, P.-Y., Fischer, G., Hyvonen, M., Hollfelder, F.

Description: Crystal Structure of Dienelactone Hydrolase-like Promiscuous Phospotriesterase P91 from Metagenomic Libraries
[[Category: Unreleased Structures]]
[[Category: Hyvonen, M]]
[[Category: Hollfelder, F]]
[[Category: Colin, P.-Y]]
[[Category: Fischer, G]]