5a5x - Revision history

OCA at 04:40, 25 May 2023

2023-05-25T04:40:45Z

←Older revision		Revision as of 04:40, 25 May 2023
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	<StructureSection load='5a5x' size='340' side='right'caption='[[5a5x]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5a5x' size='340' side='right'caption='[[5a5x]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5a5x]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa_pao1~~ Pseudomonas aeruginosa ~~pao1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5A5X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5a5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A5X FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5x OCA], [https://pdbe.org/5a5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a5x RCSB], [https://www.ebi.ac.uk/pdbsum/5a5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5x ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5a5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5x OCA], [~~http~~://pdbe.org/5a5x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5a5x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5a5x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5x ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MLTF_PSEAE MLTF_PSEAE]] Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.[HAMAP-Rule:MF_02016]	+	[https://www.uniprot.org/uniprot/MLTF_PSEAE MLTF_PSEAE] Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.[HAMAP-Rule:MF_02016]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pseudomonas aeruginosa ~~pao1~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Acebron, I]]	+	[[Category: Acebron I]]
-	[[Category: Dominguez-Gil, T]]	+	[[Category: Dominguez-Gil T]]
-	[[Category: Hermoso~~, J A]]~~	+	[[Category: Hermoso JA]]
-	~~[[Category: Cell wall recycling]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lytic transglycosilose~~]]	+

OCA at 08:50, 26 June 2019

2019-06-26T08:50:32Z

←Older revision		Revision as of 08:50, 26 June 2019
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	==Crystal Structure of Se-Met MltF from Pseudomonas aeruginosa==		==Crystal Structure of Se-Met MltF from Pseudomonas aeruginosa==
-	<StructureSection load='5a5x' size='340' side='right' caption='[[5a5x]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5a5x' size='340' side='right'caption='[[5a5x]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5a5x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A5X FirstGlance]. <br>		<table><tr><td colspan='2'>[[5a5x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A5X FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pseudomonas aeruginosa pao1]]		[[Category: Pseudomonas aeruginosa pao1]]
	[[Category: Acebron, I]]		[[Category: Acebron, I]]

OCA at 17:35, 19 October 2016

2016-10-19T17:35:58Z

←Older revision		Revision as of 17:35, 19 October 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5a5x is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of Se-Met MltF from Pseudomonas aeruginosa==
		+	<StructureSection load='5a5x' size='340' side='right' caption='[[5a5x]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5a5x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A5X FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5x OCA], [http://pdbe.org/5a5x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a5x RCSB], [http://www.ebi.ac.uk/pdbsum/5a5x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5x ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MLTF_PSEAE MLTF_PSEAE]] Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.[HAMAP-Rule:MF_02016]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria grow and divide without loss of cellular integrity. This accomplishment is notable, as a key component of their cell envelope is a surrounding glycopeptide polymer. In Gram-negative bacteria this polymer-the peptidoglycan-grows by the difference between concurrent synthesis and degradation. The regulation of the enzymatic ensemble for these activities is poorly understood. We report herein the structural basis for the control of one such enzyme, the lytic transglycosylase MltF of Pseudomonas aeruginosa. Its structure comprises two modules: an ABC-transporter-like regulatory module and a catalytic module. Occupancy of the regulatory module by peptidoglycan-derived muropeptides effects a dramatic and long-distance (40 A) conformational change, occurring over the entire protein structure, to open its active site for catalysis. This discovery of the molecular basis for the allosteric control of MltF catalysis is foundational to further study of MltF within the complex enzymatic orchestration of the dynamic peptidoglycan.

-	~~Authors:~~ Dominguez-Gil, T., ~~Acebron~~, I., ~~Hermoso~~, J.A.	+	Activation by Allostery in Cell-Wall Remodeling by a Modular Membrane-Bound Lytic Transglycosylase from Pseudomonas aeruginosa.,Dominguez-Gil T, Lee M, Acebron-Avalos I, Mahasenan KV, Hesek D, Dik DA, Byun B, Lastochkin E, Fisher JF, Mobashery S, Hermoso JA Structure. 2016 Oct 4;24(10):1729-1741. doi: 10.1016/j.str.2016.07.019. Epub 2016, Sep 8. PMID:27618662<ref>PMID:27618662</ref>

-	~~Description: Crystal Structure~~ of ~~Se-Met MltF from Pseudomonas aeruginosa~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Hermoso, J.A]]~~	+	<div class="pdbe-citations 5a5x" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Dominguez-Gil, T~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Pseudomonas aeruginosa pao1]]
	[[Category: Acebron, I]]		[[Category: Acebron, I]]
		+	[[Category: Dominguez-Gil, T]]
		+	[[Category: Hermoso, J A]]
		+	[[Category: Cell wall recycling]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lytic transglycosilose]]

OCA at 00:21, 1 December 2015

2015-12-01T00:21:03Z

←Older revision		Revision as of 00:21, 1 December 2015
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	Description: Crystal Structure of Se-Met MltF from Pseudomonas aeruginosa		Description: Crystal Structure of Se-Met MltF from Pseudomonas aeruginosa
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Hermoso, J.A]]
	[[Category: Dominguez-Gil, T]]		[[Category: Dominguez-Gil, T]]
	[[Category: Acebron, I]]		[[Category: Acebron, I]]
-	[[Category: Hermoso, J.A]]

OCA at 02:03, 22 October 2015

2015-10-22T02:03:11Z

←Older revision		Revision as of 02:03, 22 October 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5a5x is ON HOLD	+	The entry 5a5x is ON HOLD until Paper Publication

	Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.		Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.

OCA at 12:04, 12 August 2015

2015-08-12T12:04:53Z

←Older revision		Revision as of 12:04, 12 August 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5a5x is ON HOLD ~~until sometime in the future~~	+	The entry 5a5x is ON HOLD

	Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.		Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.

OCA at 17:27, 5 August 2015

2015-08-05T17:27:09Z

←Older revision		Revision as of 17:27, 5 August 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5a5x is ON HOLD	+	The entry 5a5x is ON HOLD until sometime in the future

	Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.		Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.

OCA at 12:17, 29 July 2015

2015-07-29T12:17:46Z

←Older revision		Revision as of 12:17, 29 July 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5a5x is ON HOLD ~~until sometime in the future~~	+	The entry 5a5x is ON HOLD

	Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.		Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.

OCA: Protected "5a5x" [edit=sysop:move=sysop]

2015-07-01T10:28:51Z

Protected "5a5x" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:28, 1 July 2015

OCA: New page: '''Unreleased structure''' The entry 5a5x is ON HOLD until sometime in the future Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A. Description: Crystal Structure of Se-Met MltF f...

2015-07-01T10:28:50Z

New page: '''Unreleased structure''' The entry 5a5x is ON HOLD until sometime in the future Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A. Description: Crystal Structure of Se-Met MltF f...

New page

'''Unreleased structure'''

The entry 5a5x is ON HOLD until sometime in the future

Authors: Dominguez-Gil, T., Acebron, I., Hermoso, J.A.

Description: Crystal Structure of Se-Met MltF from Pseudomonas aeruginosa
[[Category: Unreleased Structures]]
[[Category: Dominguez-Gil, T]]
[[Category: Acebron, I]]
[[Category: Hermoso, J.A]]