5b3d - Revision history

OCA at 06:37, 31 May 2023

2023-05-31T06:37:31Z

←Older revision		Revision as of 06:37, 31 May 2023
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	<StructureSection load='5b3d' size='340' side='right'caption='[[5b3d]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5b3d' size='340' side='right'caption='[[5b3d]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5b3d]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Salty Salty~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B3D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5B3D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5b3d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B3D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B3D FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flgN, STM1171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b3d OCA], [https://pdbe.org/5b3d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b3d RCSB], [https://www.ebi.ac.uk/pdbsum/5b3d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b3d ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5b3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b3d OCA], [~~http~~://pdbe.org/5b3d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5b3d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5b3d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5b3d ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FLGN_SALTY FLGN_SALTY]] Required for the efficient initiation of filament assembly.	+	[https://www.uniprot.org/uniprot/FLGN_SALTY FLGN_SALTY] Required for the efficient initiation of filament assembly.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Salty~~]]	+	[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
-	[[Category: Imada, K]]	+	[[Category: Imada K]]
-	[[Category: Kinoshita, M]]	+	[[Category: Kinoshita M]]
-	[[Category: Minamino, T]]	+	[[Category: Minamino T]]
-	[[Category: Nakanishi, Y]]	+	[[Category: Nakanishi Y]]
-	[[Category: Namba, K]]	+	[[Category: Namba K]]
-	~~[[Category: Export chaperone]]~~	+
-	~~[[Category: Flagellar type iii secretion]]~~	+
-	~~[[Category: Four-stranded coiled coil]]~~	+
-	~~[[Category: Protein transport~~]]	+

OCA at 10:02, 26 February 2020

2020-02-26T10:02:26Z

←Older revision		Revision as of 10:02, 26 February 2020
Line 1:		Line 1:

	==Structure of a flagellar type III secretion chaperone, FlgN==		==Structure of a flagellar type III secretion chaperone, FlgN==
-	<StructureSection load='5b3d' size='340' side='right' caption='[[5b3d]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5b3d' size='340' side='right'caption='[[5b3d]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5b3d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B3D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B3D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5b3d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B3D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B3D FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flgN, STM1171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b3d OCA], [http://pdbe.org/5b3d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b3d RCSB], [http://www.ebi.ac.uk/pdbsum/5b3d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b3d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b3d OCA], [http://pdbe.org/5b3d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b3d RCSB], [http://www.ebi.ac.uk/pdbsum/5b3d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b3d ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Salty]]
	[[Category: Imada, K]]		[[Category: Imada, K]]
	[[Category: Kinoshita, M]]		[[Category: Kinoshita, M]]

OCA at 05:34, 9 September 2016

2016-09-09T05:34:23Z

←Older revision		Revision as of 05:34, 9 September 2016
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[5b3d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B3D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B3D FirstGlance]. <br>		<table><tr><td colspan='2'>[[5b3d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B3D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B3D FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b3d OCA], [http://pdbe.org/5b3d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b3d RCSB], [http://www.ebi.ac.uk/pdbsum/5b3d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b3d OCA], [http://pdbe.org/5b3d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b3d RCSB], [http://www.ebi.ac.uk/pdbsum/5b3d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b3d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 15:42, 1 June 2016

2016-06-01T15:42:41Z

←Older revision		Revision as of 15:42, 1 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5b3d is ~~ON HOLD until Paper~~ Publication	+	==Structure of a flagellar type III secretion chaperone, FlgN==
		+	<StructureSection load='5b3d' size='340' side='right' caption='[[5b3d]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5b3d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B3D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B3D FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b3d OCA], [http://pdbe.org/5b3d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b3d RCSB], [http://www.ebi.ac.uk/pdbsum/5b3d PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FLGN_SALTY FLGN_SALTY]] Required for the efficient initiation of filament assembly.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacterial flagellar type III export chaperones not only act as bodyguards to protect their cognate substrates from aggregation and proteolysis in the cytoplasm but also ensure the order of export through their interactions with an export gate protein FlhA. FlgN chaperone binds to FlgK and FlgL with nanomolar affinity and transfers them to FlhA for their efficient and rapid transport for the formation of the hook-filament junction zone. However, it remains unknown how FlgN releases FlgK and FlgL at the FlhA export gate platform in a timely manner. Here, we have solved the crystal structure of Salmonella FlgN at 2.3 A resolution and carried out structure-based functional analyses. FlgN consists of three alpha helices, alpha1, alpha2 and alpha3. Helix alpha1 adopts two distinct, extended and bent conformations through the conformational change of N-loop between alpha1 and alpha2. The N-loop deletion not only increases the probability of FlgN dimer formation but also abolish the interaction between FlgN and FlgK. Highly conserved Asn-92, Asn-95 and Ile-103 residues in helix alpha3 are involved in the strong interaction with FlgK. We propose that the N-loop coordinates helical rearrangements of FlgN with the association and dissociation of its cognate substrates during their export. This article is protected by copyright. All rights reserved.

-	~~Authors: Nakanishi, Y~~., Kinoshita, M., Namba, K., Minamino, T.~~, Imada, K~~.	+	Rearrangements of alpha-helical structures of FlgN chaperone control the binding affinity for its cognate substrates during flagellar type III export.,Kinoshita M, Nakanishi Y, Furukawa Y, Namba K, Imada K, Minamino T Mol Microbiol. 2016 May 13. doi: 10.1111/mmi.13415. PMID:27178222<ref>PMID:27178222</ref>

-	~~Description: Structure of a flagellar type III secretion chaperone~~, ~~FlgN~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5b3d" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Imada, K]]		[[Category: Imada, K]]
		+	[[Category: Kinoshita, M]]
	[[Category: Minamino, T]]		[[Category: Minamino, T]]
	[[Category: Nakanishi, Y]]		[[Category: Nakanishi, Y]]
	[[Category: Namba, K]]		[[Category: Namba, K]]
-	[[Category: ~~Kinoshita, M~~]]	+	[[Category: Export chaperone]]
		+	[[Category: Flagellar type iii secretion]]
		+	[[Category: Four-stranded coiled coil]]
		+	[[Category: Protein transport]]

OCA: Protected "5b3d" [edit=sysop:move=sysop]

2016-02-26T12:23:56Z

Protected "5b3d" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:23, 26 February 2016

OCA: New page: '''Unreleased structure''' The entry 5b3d is ON HOLD until Paper Publication Authors: Nakanishi, Y., Kinoshita, M., Namba, K., Minamino, T., Imada, K. Description: Structure of a flage...

2016-02-26T12:23:55Z

New page: '''Unreleased structure''' The entry 5b3d is ON HOLD until Paper Publication Authors: Nakanishi, Y., Kinoshita, M., Namba, K., Minamino, T., Imada, K. Description: Structure of a flage...

New page

'''Unreleased structure'''

The entry 5b3d is ON HOLD until Paper Publication

Authors: Nakanishi, Y., Kinoshita, M., Namba, K., Minamino, T., Imada, K.

Description: Structure of a flagellar type III secretion chaperone, FlgN
[[Category: Unreleased Structures]]
[[Category: Imada, K]]
[[Category: Minamino, T]]
[[Category: Nakanishi, Y]]
[[Category: Namba, K]]
[[Category: Kinoshita, M]]