5b6i - Revision history

OCA at 16:01, 8 November 2023

2023-11-08T16:01:54Z

←Older revision		Revision as of 16:01, 8 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5b6i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._MA37 Streptomyces sp. MA37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B6I FirstGlance]. <br>		<table><tr><td colspan='2'>[[5b6i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._MA37 Streptomyces sp. MA37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B6I FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b6i OCA], [https://pdbe.org/5b6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b6i RCSB], [https://www.ebi.ac.uk/pdbsum/5b6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b6i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b6i OCA], [https://pdbe.org/5b6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b6i RCSB], [https://www.ebi.ac.uk/pdbsum/5b6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b6i ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:40, 31 May 2023

2023-05-31T06:40:20Z

←Older revision		Revision as of 06:40, 31 May 2023
Line 3:		Line 3:
	<StructureSection load='5b6i' size='340' side='right'caption='[[5b6i]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='5b6i' size='340' side='right'caption='[[5b6i]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5b6i]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_sp.~~_ma37~~ Streptomyces sp. ~~ma37~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B6I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5B6I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5b6i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._MA37 Streptomyces sp. MA37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B6I FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1400207 Streptomyces sp. MA37])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b6i OCA], [https://pdbe.org/5b6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b6i RCSB], [https://www.ebi.ac.uk/pdbsum/5b6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b6i ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b6i OCA], [~~http~~://pdbe.org/5b6i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5b6i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5b6i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5b6i ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/W0W999_9ACTN W0W999_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Streptomyces sp. ~~ma37~~]]	+	[[Category: Streptomyces sp. MA37]]
-	[[Category: Robinson~~, R C~~]]	+	[[Category: Robinson RC]]
-	[[Category: Xue, B]]	+	[[Category: Xue B]]
-	~~[[Category: Transferase~~]]	+

OCA at 08:47, 11 March 2020

2020-03-11T08:47:13Z

←Older revision		Revision as of 08:47, 11 March 2020
Line 1:		Line 1:

	==Structure of fluorinase from Streptomyces sp. MA37==		==Structure of fluorinase from Streptomyces sp. MA37==
-	<StructureSection load='5b6i' size='340' side='right' caption='[[5b6i]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='5b6i' size='340' side='right'caption='[[5b6i]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5b6i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B6I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5b6i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._ma37 Streptomyces sp. ma37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B6I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1400207 Streptomyces sp. MA37])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b6i OCA], [http://pdbe.org/5b6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b6i RCSB], [http://www.ebi.ac.uk/pdbsum/5b6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b6i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b6i OCA], [http://pdbe.org/5b6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b6i RCSB], [http://www.ebi.ac.uk/pdbsum/5b6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b6i ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Streptomyces sp. ma37]]
	[[Category: Robinson, R C]]		[[Category: Robinson, R C]]
	[[Category: Xue, B]]		[[Category: Xue, B]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 18:27, 26 October 2016

2016-10-26T18:27:21Z

←Older revision		Revision as of 18:27, 26 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5b6i is ~~ON HOLD until Paper~~ Publication	+	==Structure of fluorinase from Streptomyces sp. MA37==
		+	<StructureSection load='5b6i' size='340' side='right' caption='[[5b6i]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5b6i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B6I FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b6i OCA], [http://pdbe.org/5b6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b6i RCSB], [http://www.ebi.ac.uk/pdbsum/5b6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b6i ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fluorinases offer an environmentally friendly alternative for selective fluorination under mild conditions. However, their diversity is limited in nature and they have yet to be engineered through directed evolution. Herein, we report the directed evolution of the fluorinase FlA1 for improved conversion of the non-native substrate 5'-chloro-5'-deoxyadenosine (5'-ClDA) into 5'-fluoro-5'-deoxyadenosine (5'-FDA). The evolved variants, fah2081 (A279Y) and fah2114 (F213Y, A279L), were successfully applied in the radiosynthesis of 5'-[18 F]FDA, with overall radiochemical conversion (RCC) more than 3-fold higher than wild-type FlA1. Kinetic studies of the two-step reaction revealed that the variants show a significantly improved kcat value in the conversion of 5'-ClDA into S-adenosyl-l-methionine (SAM) but a reduced kcat value in the conversion of SAM into 5'-FDA.

-	~~Authors: Xue~~, B., Robinson, R.C.	+	Directed Evolution of a Fluorinase for Improved Fluorination Efficiency with a Non-native Substrate.,Sun H, Yeo WL, Lim YH, Chew X, Smith DJ, Xue B, Chan KP, Robinson RC, Robins EG, Zhao H, Ang EL Angew Chem Int Ed Engl. 2016 Oct 14. doi: 10.1002/anie.201606722. PMID:27739177<ref>PMID:27739177</ref>

-	~~Description: Structure~~ of ~~fluorinase from Streptomyces sp~~. ~~MA37~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Robinson, R.C]]	+	<div class="pdbe-citations 5b6i" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Robinson, R C]]
	[[Category: Xue, B]]		[[Category: Xue, B]]
		+	[[Category: Transferase]]

OCA at 16:45, 23 June 2016

2016-06-23T16:45:45Z

←Older revision		Revision as of 16:45, 23 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5b6i is ON HOLD	+	The entry 5b6i is ON HOLD until Paper Publication

	Authors: Xue, B., Robinson, R.C.		Authors: Xue, B., Robinson, R.C.

OCA: Protected "5b6i" [edit=sysop:move=sysop]

2016-06-20T09:03:56Z

Protected "5b6i" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:03, 20 June 2016

OCA: New page: '''Unreleased structure''' The entry 5b6i is ON HOLD Authors: Xue, B., Robinson, R.C. Description: Structure of fluorinase from Streptomyces sp. MA37 [[Category: Unreleased Structures]...

2016-06-20T09:03:54Z

New page: '''Unreleased structure''' The entry 5b6i is ON HOLD Authors: Xue, B., Robinson, R.C. Description: Structure of fluorinase from Streptomyces sp. MA37 [[Category: Unreleased Structures]...

New page

'''Unreleased structure'''

The entry 5b6i is ON HOLD

Authors: Xue, B., Robinson, R.C.

Description: Structure of fluorinase from Streptomyces sp. MA37
[[Category: Unreleased Structures]]
[[Category: Robinson, R.C]]
[[Category: Xue, B]]