5bpw - Revision history

OCA at 11:43, 9 May 2024

2024-05-09T11:43:35Z

←Older revision		Revision as of 11:43, 9 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5bpw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BPW FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bpw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BPW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bpw OCA], [https://pdbe.org/5bpw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bpw RCSB], [https://www.ebi.ac.uk/pdbsum/5bpw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bpw ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bpw OCA], [https://pdbe.org/5bpw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bpw RCSB], [https://www.ebi.ac.uk/pdbsum/5bpw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bpw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 06:03, 7 June 2023

2023-06-07T06:03:14Z

←Older revision		Revision as of 06:03, 7 June 2023
Line 1:		Line 1:

	==Atomic-resolution structures of the APC/C subunits Apc4 and the Apc5 N-terminal domain==		==Atomic-resolution structures of the APC/C subunits Apc4 and the Apc5 N-terminal domain==
-	<StructureSection load='5bpw' size='340' side='right' caption='[[5bpw]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<StructureSection load='5bpw' size='340' side='right'caption='[[5bpw]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bpw]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BPW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5BPW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bpw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BPW FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ANAPC4, APC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bpw OCA], [https://pdbe.org/5bpw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bpw RCSB], [https://www.ebi.ac.uk/pdbsum/5bpw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bpw ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5bpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bpw OCA], [~~http~~://pdbe.org/5bpw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5bpw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5bpw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5bpw ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/APC4_HUMAN APC4_HUMAN]] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.<ref>PMID:18485873</ref>	+	[https://www.uniprot.org/uniprot/APC4_HUMAN APC4_HUMAN] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.<ref>PMID:18485873</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: Barford, D]]	+	[[Category: Large Structures]]
-	[[Category: Kulkarni, K]]	+	[[Category: Barford D]]
-	[[Category: Yang, J]]	+	[[Category: Kulkarni K]]
-	[[Category: Zhang, Z]]	+	[[Category: Yang J]]
-	~~[[Category: Anaphase promoting complex]]~~	+	[[Category: Zhang Z]]
-	~~[[Category: Apc/c]]~~	+
-	~~[[Category: Apc4]]~~	+
-	~~[[Category: Cell cycle~~]]	+

OCA at 16:39, 16 November 2017

2017-11-16T16:39:25Z

←Older revision		Revision as of 16:39, 16 November 2017
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		+
	==Atomic-resolution structures of the APC/C subunits Apc4 and the Apc5 N-terminal domain==		==Atomic-resolution structures of the APC/C subunits Apc4 and the Apc5 N-terminal domain==
	<StructureSection load='5bpw' size='340' side='right' caption='[[5bpw]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='5bpw' size='340' side='right' caption='[[5bpw]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bpw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BPW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BPW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bpw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BPW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BPW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bpw OCA], [http://pdbe.org/5bpw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bpw RCSB], [http://www.ebi.ac.uk/pdbsum/5bpw PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ANAPC4, APC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bpw OCA], [http://pdbe.org/5bpw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bpw RCSB], [http://www.ebi.ac.uk/pdbsum/5bpw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bpw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Kulkarni, K]]		[[Category: Kulkarni, K]]

OCA at 06:56, 30 September 2015

2015-09-30T06:56:23Z

←Older revision		Revision as of 06:56, 30 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5bpw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BPW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BPW FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bpw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BPW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BPW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bpw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5bpw RCSB], [http://www.ebi.ac.uk/pdbsum/5bpw PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bpw OCA], [http://pdbe.org/5bpw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bpw RCSB], [http://www.ebi.ac.uk/pdbsum/5bpw PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/APC4_HUMAN APC4_HUMAN]] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.<ref>PMID:18485873</ref>		[[http://www.uniprot.org/uniprot/APC4_HUMAN APC4_HUMAN]] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.<ref>PMID:18485873</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many essential biological processes are mediated by complex molecular machines comprising multiple subunits. Knowledge on the architecture of individual subunits and their positions within the overall multimeric complex is key to understanding the molecular mechanisms of macromolecular assemblies. The anaphase-promoting complex/cyclosome (APC/C) is a large multisubunit complex that regulates cell cycle progression by ubiquitinating cell cycle proteins for proteolysis by the proteasome. The holo-complex is composed of 15 different proteins that assemble to generate a complex of 20 subunits. Here, we describe the crystal structures of Apc4 and the N-terminal domain of Apc5 (Apc5(N)). Apc4 comprises a WD40 domain split by a long alpha-helical domain, whereas Apc5(N) has an alpha-helical fold. In a separate study, we had fitted these atomic models to a 3.6-A-resolution cryo-electron microscopy map of the APC/C. We describe how, in the context of the APC/C, regions of Apc4 disordered in the crystal assume order through contacts to Apc5, whereas Apc5(N) shows small conformational changes relative to its crystal structure. We discuss the complementary approaches of high-resolution electron microscopy and protein crystallography to the structure determination of subunits of multimeric complexes.
		+
		+	Atomic-Resolution Structures of the APC/C Subunits Apc4 and the Apc5 N-Terminal Domain.,Cronin NB, Yang J, Zhang Z, Kulkarni K, Chang L, Yamano H, Barford D J Mol Biol. 2015 Oct 9;427(20):3300-15. doi: 10.1016/j.jmb.2015.08.023. Epub 2015, Sep 4. PMID:26343760<ref>PMID:26343760</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5bpw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:34, 2 September 2015

2015-09-02T12:34:43Z

←Older revision		Revision as of 12:34, 2 September 2015
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	==Atomic-resolution structures of the APC/C subunits Apc4 and the Apc5 N-terminal domain==
-		+	<StructureSection load='5bpw' size='340' side='right' caption='[[5bpw]], [[Resolution\|resolution]] 3.40Å' scene=''>
-	~~The entry 5bpw is ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5bpw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BPW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BPW FirstGlance]. <br>
-	~~Authors: Kulkarni, K., Yang, J., Zhang, Z., Barford, D.~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bpw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5bpw RCSB], [http://www.ebi.ac.uk/pdbsum/5bpw PDBsum]</span></td></tr>
-		+	</table>
-	~~Description:~~ Atomic-resolution structures of the APC/C subunits Apc4 and the Apc5 N-terminal domain	+	== Function ==
-	[[~~Category: Unreleased Structures~~]]	+	[[http://www.uniprot.org/uniprot/APC4_HUMAN APC4_HUMAN]] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.<ref>PMID:18485873</ref>
-	[[~~Category~~: ~~Zhang, Z~~]]	+	== References ==
-	[[~~Category~~: ~~Yang~~, J]]	+	<references/>
-	[[~~Category~~: ~~Kulkarni, K~~]]	+	__TOC__
		+	</StructureSection>
	[[Category: Barford, D]]		[[Category: Barford, D]]
		+	[[Category: Kulkarni, K]]
		+	[[Category: Yang, J]]
		+	[[Category: Zhang, Z]]
		+	[[Category: Anaphase promoting complex]]
		+	[[Category: Apc/c]]
		+	[[Category: Apc4]]
		+	[[Category: Cell cycle]]

OCA at 12:52, 10 June 2015

2015-06-10T12:52:21Z

←Older revision		Revision as of 12:52, 10 June 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5bpw is ON HOLD	+	The entry 5bpw is ON HOLD until Paper Publication

	Authors: Kulkarni, K., Yang, J., Zhang, Z., Barford, D.		Authors: Kulkarni, K., Yang, J., Zhang, Z., Barford, D.

OCA: Protected "5bpw" [edit=sysop:move=sysop]

2015-06-03T11:47:42Z

Protected "5bpw" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:47, 3 June 2015

OCA: New page: '''Unreleased structure''' The entry 5bpw is ON HOLD Authors: Kulkarni, K., Yang, J., Zhang, Z., Barford, D. Description: Atomic-resolution structures of the APC/C subunits Apc4 and th...

2015-06-03T11:47:40Z

New page: '''Unreleased structure''' The entry 5bpw is ON HOLD Authors: Kulkarni, K., Yang, J., Zhang, Z., Barford, D. Description: Atomic-resolution structures of the APC/C subunits Apc4 and th...

New page

'''Unreleased structure'''

The entry 5bpw is ON HOLD

Authors: Kulkarni, K., Yang, J., Zhang, Z., Barford, D.

Description: Atomic-resolution structures of the APC/C subunits Apc4 and the Apc5 N-terminal domain
[[Category: Unreleased Structures]]
[[Category: Zhang, Z]]
[[Category: Yang, J]]
[[Category: Kulkarni, K]]
[[Category: Barford, D]]