5bs9 - Revision history

OCA at 08:29, 27 September 2023

2023-09-27T08:29:28Z

←Older revision		Revision as of 08:29, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5bs9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BS9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bs9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BS9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.98Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bs9 OCA], [https://pdbe.org/5bs9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bs9 RCSB], [https://www.ebi.ac.uk/pdbsum/5bs9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bs9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bs9 OCA], [https://pdbe.org/5bs9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bs9 RCSB], [https://www.ebi.ac.uk/pdbsum/5bs9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bs9 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:06, 7 June 2023

2023-06-07T06:06:28Z

←Older revision		Revision as of 06:06, 7 June 2023
Line 3:		Line 3:
	<StructureSection load='5bs9' size='340' side='right'caption='[[5bs9]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='5bs9' size='340' side='right'caption='[[5bs9]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bs9]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BS9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5BS9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bs9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BS9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bsc\|5bsc]], [[5bsi\|5bsi]], [[5bsj\|5bsj]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bs9 OCA], [https://pdbe.org/5bs9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bs9 RCSB], [https://www.ebi.ac.uk/pdbsum/5bs9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bs9 ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MIF, GLIF, MMIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5bs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bs9 OCA], [~~http~~://pdbe.org/5bs9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5bs9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5bs9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5bs9 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:[~~http~~://omim.org/entry/604302 604302]]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.	+	[https://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:[https://omim.org/entry/604302 604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.<ref>PMID:15908412</ref> <ref>PMID:17443469</ref>	+	[https://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN] Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.<ref>PMID:15908412</ref> <ref>PMID:17443469</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 29:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lolis, E]]	+	[[Category: Lolis E]]
-	[[Category: Pantouris, G]]	+	[[Category: Pantouris G]]
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Mutation]]~~	+
-	~~[[Category: Surface~~]]	+

OCA at 10:59, 18 March 2020

2020-03-18T10:59:02Z

←Older revision		Revision as of 10:59, 18 March 2020
Line 1:		Line 1:
		+
	==Crystal structure of N109A mutant of human macrophage migration inhibitory factor==		==Crystal structure of N109A mutant of human macrophage migration inhibitory factor==
-	<StructureSection load='5bs9' size='340' side='right' caption='[[5bs9]], [[Resolution\|resolution]] 1.98Å' scene=''>	+	<StructureSection load='5bs9' size='340' side='right'caption='[[5bs9]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bs9]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BS9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BS9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bs9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BS9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BS9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bsc\|5bsc]], [[5bsi\|5bsi]], [[5bsj\|5bsj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bsc\|5bsc]], [[5bsi\|5bsi]], [[5bsj\|5bsj]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bs9 OCA], [http://pdbe.org/5bs9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bs9 RCSB], [http://www.ebi.ac.uk/pdbsum/5bs9 PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MIF, GLIF, MMIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bs9 OCA], [http://pdbe.org/5bs9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bs9 RCSB], [http://www.ebi.ac.uk/pdbsum/5bs9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bs9 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 20:		Line 22:
	</div>		</div>
	<div class="pdbe-citations 5bs9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5bs9" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Macrophage inhibitory factor 3D structures\|Macrophage inhibitory factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Lolis, E]]		[[Category: Lolis, E]]
	[[Category: Pantouris, G]]		[[Category: Pantouris, G]]

OCA at 14:36, 30 September 2015

2015-09-30T14:36:14Z

←Older revision		Revision as of 14:36, 30 September 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of N109A mutant of human macrophage migration inhibitory factor==
		+	<StructureSection load='5bs9' size='340' side='right' caption='[[5bs9]], [[Resolution\|resolution]] 1.98Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5bs9]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BS9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BS9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bsc\|5bsc]], [[5bsi\|5bsi]], [[5bsj\|5bsj]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bs9 OCA], [http://pdbe.org/5bs9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bs9 RCSB], [http://www.ebi.ac.uk/pdbsum/5bs9 PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:[http://omim.org/entry/604302 604302]]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.<ref>PMID:15908412</ref> <ref>PMID:17443469</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	For more than 15 years, the tautomerase active site of macrophage migration inhibitory factor (MIF) and its catalytic residue Pro1 have been being targeted for the development of therapeutics that block activation of its cell surface receptor, CD74. Neither the biological role of the MIF catalytic site nor the mechanistic details of CD74 activation are well understood. The inherently unstable structure of CD74 remains the biggest obstacle in structural studies with MIF for understanding the basis of CD74 activation. Using a novel approach, we elucidate the mechanistic details that control activation of CD74 by MIF surface residues and identify structural parameters of inhibitors that reduce CD74 biological activation. We also find that N-terminal mutants located deep in the catalytic site affect surface residues immediately outside the catalytic site, which are responsible for reduction of CD74 activation.

-	~~The entry 5bs9 is ON HOLD until Paper Publication~~	+	An Analysis of MIF Structural Features that Control Functional Activation of CD74.,Pantouris G, Syed MA, Fan C, Rajasekaran D, Cho TY, Rosenberg EM Jr, Bucala R, Bhandari V, Lolis EJ Chem Biol. 2015 Sep 17;22(9):1197-205. doi: 10.1016/j.chembiol.2015.08.006. Epub , 2015 Sep 10. PMID:26364929<ref>PMID:26364929</ref>

-	~~Authors: Pantouris~~, G.~~, Lolis, E~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of N109A mutant of human macrophage migration inhibitory factor~~	+	<div class="pdbe-citations 5bs9" style="background-color:#fffaf0;"></div>
-	~~[[Category: Unreleased Structures]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Lolis, E]]		[[Category: Lolis, E]]
	[[Category: Pantouris, G]]		[[Category: Pantouris, G]]
		+	[[Category: Isomerase]]
		+	[[Category: Mutation]]
		+	[[Category: Surface]]

OCA at 11:37, 24 June 2015

2015-06-24T11:37:50Z

←Older revision		Revision as of 11:37, 24 June 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5bs9 is ON HOLD	+	The entry 5bs9 is ON HOLD until Paper Publication

	Authors: Pantouris, G., Lolis, E.		Authors: Pantouris, G., Lolis, E.

OCA: Protected "5bs9" [edit=sysop:move=sysop]

2015-06-10T12:59:28Z

Protected "5bs9" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:59, 10 June 2015

OCA: New page: '''Unreleased structure''' The entry 5bs9 is ON HOLD Authors: Pantouris, G., Lolis, E. Description: Crystal structure of N109A mutant of human macrophage migration inhibitory factor [[...

2015-06-10T12:59:27Z

New page: '''Unreleased structure''' The entry 5bs9 is ON HOLD Authors: Pantouris, G., Lolis, E. Description: Crystal structure of N109A mutant of human macrophage migration inhibitory factor [[...

New page

'''Unreleased structure'''

The entry 5bs9 is ON HOLD

Authors: Pantouris, G., Lolis, E.

Description: Crystal structure of N109A mutant of human macrophage migration inhibitory factor
[[Category: Unreleased Structures]]
[[Category: Lolis, E]]
[[Category: Pantouris, G]]