5bse - Revision history

OCA at 08:29, 27 September 2023

2023-09-27T08:29:38Z

←Older revision		Revision as of 08:29, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5bse]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bse]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BSE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bse OCA], [https://pdbe.org/5bse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bse RCSB], [https://www.ebi.ac.uk/pdbsum/5bse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bse ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bse OCA], [https://pdbe.org/5bse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bse RCSB], [https://www.ebi.ac.uk/pdbsum/5bse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bse ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:06, 7 June 2023

2023-06-07T06:06:45Z

←Older revision		Revision as of 06:06, 7 June 2023
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	==Crystal structure of Medicago truncatula (delta)1-Pyrroline-5-Carboxylate Reductase (MtP5CR)==		==Crystal structure of Medicago truncatula (delta)1-Pyrroline-5-Carboxylate Reductase (MtP5CR)==
-	<StructureSection load='5bse' size='340' side='right' caption='[[5bse]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='5bse' size='340' side='right'caption='[[5bse]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bse]] is a 10 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Barrel_medic Barrel medic~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BSE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5BSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bse]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BSE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bsf\|5bsf]], [[5bsg\|5bsg]], [[5bsh\|5bsh]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bse OCA], [https://pdbe.org/5bse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bse RCSB], [https://www.ebi.ac.uk/pdbsum/5bse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bse ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MTR_7g090160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3880 Barrel medic])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrroline-5-carboxylate_reductase Pyrroline-5-carboxylate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.2 1.5.1.2] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5bse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bse OCA], [~~http~~://pdbe.org/5bse PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5bse RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5bse PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5bse ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G7KRM5_MEDTR G7KRM5_MEDTR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Barrel medic~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Pyrroline-5-carboxylate reductase~~]]	+	[[Category: Medicago truncatula]]
-	[[Category: Dauter, Z]]	+	[[Category: Dauter Z]]
-	[[Category: Forlani, G]]	+	[[Category: Forlani G]]
-	[[Category: Nocek, B]]	+	[[Category: Nocek B]]
-	[[Category: Ruszkowski, M]]	+	[[Category: Ruszkowski M]]
-	~~[[Category: Decamer]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: P5c]]~~	+
-	~~[[Category: Plant protein]]~~	+
-	~~[[Category: Proline biosynthesis~~]]	+

OCA at 14:00, 16 November 2017

2017-11-16T14:00:16Z

←Older revision		Revision as of 14:00, 16 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of Medicago truncatula (delta)1-Pyrroline-5-Carboxylate Reductase (MtP5CR)==		==Crystal structure of Medicago truncatula (delta)1-Pyrroline-5-Carboxylate Reductase (MtP5CR)==
	<StructureSection load='5bse' size='340' side='right' caption='[[5bse]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5bse' size='340' side='right' caption='[[5bse]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5bse]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5bse]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Barrel_medic Barrel medic]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BSE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bsf\|5bsf]], [[5bsg\|5bsg]], [[5bsh\|5bsh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bsf\|5bsf]], [[5bsg\|5bsg]], [[5bsh\|5bsh]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MTR_7g090160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3880 Barrel medic])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrroline-5-carboxylate_reductase Pyrroline-5-carboxylate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.2 1.5.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrroline-5-carboxylate_reductase Pyrroline-5-carboxylate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.2 1.5.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bse OCA], [http://pdbe.org/5bse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bse RCSB], [http://www.ebi.ac.uk/pdbsum/5bse PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bse OCA], [http://pdbe.org/5bse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bse RCSB], [http://www.ebi.ac.uk/pdbsum/5bse PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bse ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5bse" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5bse" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Pyrroline-5-carboxylate reductase\|Pyrroline-5-carboxylate reductase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Barrel medic]]
	[[Category: Pyrroline-5-carboxylate reductase]]		[[Category: Pyrroline-5-carboxylate reductase]]
	[[Category: Dauter, Z]]		[[Category: Dauter, Z]]

OCA at 10:10, 2 December 2015

2015-12-02T10:10:02Z

←Older revision		Revision as of 10:10, 2 December 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of Medicago truncatula (delta)1-Pyrroline-5-Carboxylate Reductase (MtP5CR)==
		+	<StructureSection load='5bse' size='340' side='right' caption='[[5bse]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5bse]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BSE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bsf\|5bsf]], [[5bsg\|5bsg]], [[5bsh\|5bsh]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrroline-5-carboxylate_reductase Pyrroline-5-carboxylate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.2 1.5.1.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bse OCA], [http://pdbe.org/5bse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bse RCSB], [http://www.ebi.ac.uk/pdbsum/5bse PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The two pathways for proline biosynthesis in higher plants share the last step, the conversion of delta1-pyrroline-5-carboxylate (P5C) to L-proline, which is catalyzed by P5C reductase (P5CR, EC 1.5.1.2) with the use of NAD(P)H as a coenzyme. There is increasing amount of evidence to suggest a complex regulation of P5CR activity at the post-translational level, yet the molecular basis of these mechanisms is unknown. Here we report the three-dimensional structure of the P5CR enzyme from the model legume Medicago truncatula (Mt). The crystal structures of unliganded MtP5CR decamer, and its complexes with the products NAD+, NADP+, and L-proline were refined using x-ray diffraction data (at 1.7, 1.85, 1.95, and 2.1 A resolution, respectively). Based on the presented structural data, the coenzyme preference for NADPH over NADH was explained, and NADPH is suggested to be the only coenzyme used by MtP5CR in vivo. Furthermore, the insensitivity of MtP5CR to feed-back inhibition by proline, revealed by enzymatic analysis, was correlated with structural features. Additionally, a mechanism for the modulation of enzyme activity by chloride anions is discussed, as well as the rationale for the possible development of effective enzyme inhibitors.

-	The ~~entry 5bse is ON HOLD until Paper Publication~~	+	The structure of delta-pyrroline-5-carboxylate reductase provides new insights into regulation of proline biosynthesis in plants.,Ruszkowski M, Nocek B, Forlani G, Dauter Z Front Plant Sci. 2015 Oct 30;6:869. eCollection 2015. PMID:26579138<ref>PMID:26579138</ref>

-	~~Authors: Ruszkowski~~, M.~~, Nocek, B~~.~~, Forlani, G., Dauter, Z~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of Medicago truncatula (delta)1~~-~~Pyrroline~~-~~5-Carboxylate Reductase (MtP5CR)~~	+	<div class="pdbe-citations 5bse" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Pyrroline-5-carboxylate reductase]]
	[[Category: Dauter, Z]]		[[Category: Dauter, Z]]
		+	[[Category: Forlani, G]]
	[[Category: Nocek, B]]		[[Category: Nocek, B]]
-	[[Category: Forlani, G]]
	[[Category: Ruszkowski, M]]		[[Category: Ruszkowski, M]]
		+	[[Category: Decamer]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: P5c]]
		+	[[Category: Plant protein]]
		+	[[Category: Proline biosynthesis]]

OCA at 13:51, 17 June 2015

2015-06-17T13:51:14Z

←Older revision		Revision as of 13:51, 17 June 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5bse is ON HOLD	+	The entry 5bse is ON HOLD until Paper Publication

	Authors: Ruszkowski, M., Nocek, B., Forlani, G., Dauter, Z.		Authors: Ruszkowski, M., Nocek, B., Forlani, G., Dauter, Z.

OCA: Protected "5bse" [edit=sysop:move=sysop]

2015-06-10T12:59:41Z

Protected "5bse" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:59, 10 June 2015

OCA: New page: '''Unreleased structure''' The entry 5bse is ON HOLD Authors: Ruszkowski, M., Nocek, B., Forlani, G., Dauter, Z. Description: Crystal structure of Medicago truncatula (delta)1-Pyrrolin...

2015-06-10T12:59:40Z

New page: '''Unreleased structure''' The entry 5bse is ON HOLD Authors: Ruszkowski, M., Nocek, B., Forlani, G., Dauter, Z. Description: Crystal structure of Medicago truncatula (delta)1-Pyrrolin...

New page

'''Unreleased structure'''

The entry 5bse is ON HOLD

Authors: Ruszkowski, M., Nocek, B., Forlani, G., Dauter, Z.

Description: Crystal structure of Medicago truncatula (delta)1-Pyrroline-5-Carboxylate Reductase (MtP5CR)
[[Category: Unreleased Structures]]
[[Category: Dauter, Z]]
[[Category: Nocek, B]]
[[Category: Forlani, G]]
[[Category: Ruszkowski, M]]