5c3n - Revision history

OCA at 16:06, 8 November 2023

2023-11-08T16:06:25Z

←Older revision		Revision as of 16:06, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5c3n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Middle_East_respiratory_syndrome-related_coronavirus Middle East respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C3N FirstGlance]. <br>		<table><tr><td colspan='2'>[[5c3n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Middle_East_respiratory_syndrome-related_coronavirus Middle East respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C3N FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c3n OCA], [https://pdbe.org/5c3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c3n RCSB], [https://www.ebi.ac.uk/pdbsum/5c3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c3n ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c3n OCA], [https://pdbe.org/5c3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c3n RCSB], [https://www.ebi.ac.uk/pdbsum/5c3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c3n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 06:18, 7 June 2023

2023-06-07T06:18:48Z

←Older revision		Revision as of 06:18, 7 June 2023
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	<StructureSection load='5c3n' size='340' side='right'caption='[[5c3n]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='5c3n' size='340' side='right'caption='[[5c3n]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5c3n]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Mers Mers~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C3N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5C3N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5c3n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Middle_East_respiratory_syndrome-related_coronavirus Middle East respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C3N FirstGlance]. <br>
-	</td></tr>~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">orf1ab ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1335626 MERS])</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c3n OCA], [https://pdbe.org/5c3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c3n RCSB], [https://www.ebi.ac.uk/pdbsum/5c3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c3n ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5c3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c3n OCA], [~~http~~://pdbe.org/5c3n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5c3n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5c3n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5c3n ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/V9TU05_MERS V9TU05_MERS] Catalytic subunit of viral RNA capping enzyme which catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. The kinase-like NiRAN domain of NSP12 transfers RNA to the amino terminus of NSP9, forming a covalent RNA-protein intermediate. Subsequently, the NiRAN domain transfers RNA to GDP, forming the core cap structure GpppA-RNA. The NSP14 and NSP16 methyltransferases then add methyl groups to form functional cap structures.[ARBA:ARBA00034461]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[~~SARS Coronavirus Main Proteinase~~\|~~SARS Coronavirus Main Proteinase~~]]	+	*[[Virus protease 3D structures\|Virus protease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Mers~~]]	+	[[Category: Middle East respiratory syndrome-related coronavirus]]
-	[[Category: Cheng~~, S C~~]]	+	[[Category: Cheng SC]]
-	[[Category: Chou~~, C Y]]~~	+	[[Category: Chou CY]]
-	~~[[Category: 3cl protease]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Main protease]]~~	+
-	~~[[Category: Mers coronavirus~~]]	+

OCA at 11:10, 18 March 2020

2020-03-18T11:10:24Z

←Older revision		Revision as of 11:10, 18 March 2020
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		+
	==Crystal structure of MERS coronavirus main protease in spacegroup C2221==		==Crystal structure of MERS coronavirus main protease in spacegroup C2221==
-	<StructureSection load='5c3n' size='340' side='right' caption='[[5c3n]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='5c3n' size='340' side='right'caption='[[5c3n]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5c3n]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C3N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5c3n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mers Mers]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C3N FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c3n OCA], [http://pdbe.org/5c3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c3n RCSB], [http://www.ebi.ac.uk/pdbsum/5c3n PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">orf1ab ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1335626 MERS])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c3n OCA], [http://pdbe.org/5c3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c3n RCSB], [http://www.ebi.ac.uk/pdbsum/5c3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c3n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5c3n" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5c3n" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[SARS Coronavirus Main Proteinase\|SARS Coronavirus Main Proteinase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mers]]
	[[Category: Cheng, S C]]		[[Category: Cheng, S C]]
	[[Category: Chou, C Y]]		[[Category: Chou, C Y]]

OCA at 19:31, 30 December 2015

2015-12-30T19:31:42Z

←Older revision		Revision as of 19:31, 30 December 2015
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-	'''~~Unreleased~~ structure'''	+	==Crystal structure of MERS coronavirus main protease in spacegroup C2221==
		+	<StructureSection load='5c3n' size='340' side='right' caption='[[5c3n]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5c3n]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C3N FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c3n OCA], [http://pdbe.org/5c3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c3n RCSB], [http://www.ebi.ac.uk/pdbsum/5c3n PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: A highly pathogenic human coronavirus (CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), has emerged in Jeddah and other places in Saudi Arabia, and has quickly spread to European and Asian countries since September 2012. Up to the 1st October 2015 it has infected at least 1593 people with a global fatality rate of about 35%. Studies to understand the virus are necessary and urgent. In the present study, MERS-CoV main protease (Mpro) is expressed; the dimerization of the protein and its relationship to catalysis are investigated. METHODS AND RESULTS: The crystal structure of MERS-CoV Mpro indicates that it shares a similar scaffold to that of other coronaviral Mpro and consists of chymotrypsin-like domains I and II and a helical domain III of five helices. Analytical ultracentrifugation analysis demonstrated that MERS-CoV Mpro undergoes a monomer to dimer conversion in the presence of a peptide substrate. Glu169 is a key residue and plays a dual role in both dimerization and catalysis. The mutagenesis of other residues found on the dimerization interface indicate that dimerization of MERS-CoV Mpro is required for its catalytic activity. One mutation, M298R, resulted in a stable dimer with a higher level of proteolytic activity than the wild-type enzyme. CONCLUSIONS: MERS-CoV Mpro shows substrate-induced dimerization and potent proteolytic activity. A critical assessment of the residues important to these processes provides insights into the correlation between dimerization and catalysis within the coronaviral Mpro family.

-	~~The entry 5c3n is ON HOLD until Paper Publication~~	+	Critical Assessment of the Important Residues Involved in the Dimerization and Catalysis of MERS Coronavirus Main Protease.,Ho BL, Cheng SC, Shi L, Wang TY, Ho KI, Chou CY PLoS One. 2015 Dec 14;10(12):e0144865. doi: 10.1371/journal.pone.0144865., eCollection 2015. PMID:26658006<ref>PMID:26658006</ref>

-	~~Authors: Chou~~, ~~C.Y~~.~~, Cheng,~~ S.C.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of MERS coronavirus main protease in spacegroup C2221~~	+	<div class="pdbe-citations 5c3n" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
-	[[Category: Chou, C.Y]]	+	<references/>
-	[[Category: ~~Cheng, S.C~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Cheng, S C]]
		+	[[Category: Chou, C Y]]
		+	[[Category: 3cl protease]]
		+	[[Category: Hydrolase]]
		+	[[Category: Main protease]]
		+	[[Category: Mers coronavirus]]

OCA: Protected "5c3n" [edit=sysop:move=sysop]

2015-06-24T12:02:24Z

Protected "5c3n" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:02, 24 June 2015

OCA: New page: '''Unreleased structure''' The entry 5c3n is ON HOLD until Paper Publication Authors: Chou, C.Y., Cheng, S.C. Description: Crystal structure of MERS coronavirus main protease in spaceg...

2015-06-24T12:02:22Z

New page: '''Unreleased structure''' The entry 5c3n is ON HOLD until Paper Publication Authors: Chou, C.Y., Cheng, S.C. Description: Crystal structure of MERS coronavirus main protease in spaceg...

New page

'''Unreleased structure'''

The entry 5c3n is ON HOLD until Paper Publication

Authors: Chou, C.Y., Cheng, S.C.

Description: Crystal structure of MERS coronavirus main protease in spacegroup C2221
[[Category: Unreleased Structures]]
[[Category: Chou, C.Y]]
[[Category: Cheng, S.C]]