5ce6 - Revision history

OCA at 16:08, 8 November 2023

2023-11-08T16:08:49Z

←Older revision		Revision as of 16:08, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ce6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cicer_arietinum Cicer arietinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CE6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ce6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cicer_arietinum Cicer arietinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CE6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ce6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ce6 OCA], [https://pdbe.org/5ce6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ce6 RCSB], [https://www.ebi.ac.uk/pdbsum/5ce6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ce6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ce6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ce6 OCA], [https://pdbe.org/5ce6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ce6 RCSB], [https://www.ebi.ac.uk/pdbsum/5ce6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ce6 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:01, 14 June 2023

2023-06-14T12:01:02Z

←Older revision		Revision as of 12:01, 14 June 2023
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	==N-terminal domain of FACT complex subunit SPT16 from Cicer arietinum (chickpea)==		==N-terminal domain of FACT complex subunit SPT16 from Cicer arietinum (chickpea)==
-	<StructureSection load='5ce6' size='340' side='right' caption='[[5ce6]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='5ce6' size='340' side='right'caption='[[5ce6]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ce6]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cicar Cicar~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CE6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5CE6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ce6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cicer_arietinum Cicer arietinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CE6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LOC101489759 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3827 CICAR])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ce6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ce6 OCA], [https://pdbe.org/5ce6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ce6 RCSB], [https://www.ebi.ac.uk/pdbsum/5ce6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ce6 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ce6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ce6 OCA], [~~http~~://pdbe.org/5ce6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ce6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ce6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ce6 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A182DWB2_CICAR A0A182DWB2_CICAR] Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.[RuleBase:RU367052]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cicar~~]]	+	[[Category: Cicer arietinum]]
-	[[Category: Are~~, V N~~]]	+	[[Category: Large Structures]]
-	[[Category: Ghosh, B]]	+	[[Category: Are VN]]
-	[[Category: Kumar, A]]	+	[[Category: Ghosh B]]
-	[[Category: Makde, R]]	+	[[Category: Kumar A]]
-	~~[[Category: Fact complex]]~~	+	[[Category: Makde R]]
-	~~[[Category: H3-h4 binding]]~~	+
-	~~[[Category: Histone chaperone]]~~	+
-	~~[[Category: Spt16]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 07:29, 4 July 2018

2018-07-04T07:29:59Z

←Older revision		Revision as of 07:29, 4 July 2018
Line 3:		Line 3:
	<StructureSection load='5ce6' size='340' side='right' caption='[[5ce6]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5ce6' size='340' side='right' caption='[[5ce6]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ce6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CE6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ce6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cicar Cicar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CE6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ce6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ce6 OCA], [http://pdbe.org/5ce6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ce6 RCSB], [http://www.ebi.ac.uk/pdbsum/5ce6 PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LOC101489759 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3827 CICAR])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ce6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ce6 OCA], [http://pdbe.org/5ce6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ce6 RCSB], [http://www.ebi.ac.uk/pdbsum/5ce6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ce6 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cicar]]
	[[Category: Are, V N]]		[[Category: Are, V N]]
	[[Category: Ghosh, B]]		[[Category: Ghosh, B]]

OCA at 11:24, 12 May 2016

2016-05-12T11:24:02Z

←Older revision		Revision as of 11:24, 12 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ce6 is ~~ON HOLD until Paper~~ Publication	+	==N-terminal domain of FACT complex subunit SPT16 from Cicer arietinum (chickpea)==
		+	<StructureSection load='5ce6' size='340' side='right' caption='[[5ce6]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ce6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CE6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ce6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ce6 OCA], [http://pdbe.org/5ce6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ce6 RCSB], [http://www.ebi.ac.uk/pdbsum/5ce6 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The facilitates chromatin transcription (FACT) complex, a heterodimer of SSRP1 and Spt16 proteins, is an essential histone chaperone that transiently reorganizes nucleosomes during transcription, replication and repair. N-terminal domain of Spt16 subunit (Spt16N) is strictly conserved in all the known Spt16 orthologs. Genetic studies in yeast have revealed a partially redundant role of Spt16N for the FACT functionality. Here, we report the crystal structure of Spt16N from a plant origin (Spt16Nca, Cicer arietinum) and its comparisons with the known Spt16N structures from yeasts and human. The inter-domain angle in Spt16Nca is significantly different from that of the yeast and human Spt16N structures. Normal mode analysis and classical molecular dynamics simulations reveal inter-domain movement in Spt16Nca and later also shows conformational flexibility of the critical loops. Spt16Nca binds to histone H3/H4 complex, similar to its orthologs from yeast and human origins. Further, conservation of electrostatic surface potentials in Spt16N structures from evolutionary distinct domains of eukaryotes (plant, human and fungi) have provided the potential sites on Spt16N for histone interactions. The structural comparisons with M24 peptidases show that the hydrophobic pocket shielded by a flexible loop of C-terminal domain of Spt16N that may be functionally important.

-	~~Authors: Are, V~~., ~~Ghosh~~, B., ~~Ashwani~~, ~~K., Jamdar, S.~~, Makde~~, R~~.	+	Crystal structure and dynamics of Spt16N-domain of FACT complex from Cicer arietinum.,Are VN, Ghosh B, Kumar A, Gadre R, Makde RD Int J Biol Macromol. 2016 Mar 16;88:36-43. doi: 10.1016/j.ijbiomac.2016.03.029. PMID:26995613<ref>PMID:26995613</ref>

-	~~Description: N-terminal domain~~ of ~~FACT complex subunit SPT16 from Cicer arietinum (chickpea)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Are, V]]~~	+	<div class="pdbe-citations 5ce6" style="background-color:#fffaf0;"></div>
-	~~[[Category: Ashwani, K]]~~	+	== References ==
-	~~[[Category: Makde, R]]~~	+	<references/>
-	[[Category: ~~Jamdar~~, S]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Are, V N]]
	[[Category: Ghosh, B]]		[[Category: Ghosh, B]]
		+	[[Category: Kumar, A]]
		+	[[Category: Makde, R]]
		+	[[Category: Fact complex]]
		+	[[Category: H3-h4 binding]]
		+	[[Category: Histone chaperone]]
		+	[[Category: Spt16]]
		+	[[Category: Transcription]]

OCA at 10:29, 16 December 2015

2015-12-16T10:29:04Z

←Older revision		Revision as of 10:29, 16 December 2015
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Are, V]]		[[Category: Are, V]]
-	[[Category: Jamdar, S]]
	[[Category: Ashwani, K]]		[[Category: Ashwani, K]]
	[[Category: Makde, R]]		[[Category: Makde, R]]
		+	[[Category: Jamdar, S]]
	[[Category: Ghosh, B]]		[[Category: Ghosh, B]]

OCA at 10:34, 2 September 2015

2015-09-02T10:34:22Z

←Older revision		Revision as of 10:34, 2 September 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ce6 is ON HOLD	+	The entry 5ce6 is ON HOLD until Paper Publication

	Authors: Are, V., Ghosh, B., Ashwani, K., Jamdar, S., Makde, R.		Authors: Are, V., Ghosh, B., Ashwani, K., Jamdar, S., Makde, R.

OCA: Protected "5ce6" [edit=sysop:move=sysop]

2015-07-15T12:26:44Z

Protected "5ce6" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:26, 15 July 2015

OCA: New page: '''Unreleased structure''' The entry 5ce6 is ON HOLD Authors: Are, V., Ghosh, B., Ashwani, K., Jamdar, S., Makde, R. Description: N-terminal domain of FACT complex subunit SPT16 from C...

2015-07-15T12:26:42Z

New page: '''Unreleased structure''' The entry 5ce6 is ON HOLD Authors: Are, V., Ghosh, B., Ashwani, K., Jamdar, S., Makde, R. Description: N-terminal domain of FACT complex subunit SPT16 from C...

New page

'''Unreleased structure'''

The entry 5ce6 is ON HOLD

Authors: Are, V., Ghosh, B., Ashwani, K., Jamdar, S., Makde, R.

Description: N-terminal domain of FACT complex subunit SPT16 from Cicer arietinum (chickpea)
[[Category: Unreleased Structures]]
[[Category: Are, V]]
[[Category: Jamdar, S]]
[[Category: Ashwani, K]]
[[Category: Makde, R]]
[[Category: Ghosh, B]]