5cj5 - Revision history

OCA at 11:20, 10 January 2024

2024-01-10T11:20:34Z

←Older revision		Revision as of 11:20, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5cj5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_thermoresistibile_ATCC_19527 Mycolicibacterium thermoresistibile ATCC 19527]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CJ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CJ5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5cj5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_thermoresistibile_ATCC_19527 Mycolicibacterium thermoresistibile ATCC 19527]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CJ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CJ5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cj5 OCA], [https://pdbe.org/5cj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cj5 RCSB], [https://www.ebi.ac.uk/pdbsum/5cj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cj5 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.13Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cj5 OCA], [https://pdbe.org/5cj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cj5 RCSB], [https://www.ebi.ac.uk/pdbsum/5cj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cj5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 12:08, 14 June 2023

2023-06-14T12:08:53Z

←Older revision		Revision as of 12:08, 14 June 2023
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	<StructureSection load='5cj5' size='340' side='right'caption='[[5cj5]], [[Resolution\|resolution]] 3.13Å' scene=''>		<StructureSection load='5cj5' size='340' side='right'caption='[[5cj5]], [[Resolution\|resolution]] 3.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5cj5]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myct3 Myct3~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CJ5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5CJ5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5cj5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_thermoresistibile_ATCC_19527 Mycolicibacterium thermoresistibile ATCC 19527]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CJ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CJ5 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glgE, KEK_12948 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1078020 MYCT3])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cj5 OCA], [https://pdbe.org/5cj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cj5 RCSB], [https://www.ebi.ac.uk/pdbsum/5cj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cj5 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Starch_synthase_(maltosyl-transferring) Starch synthase (maltosyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.16 2.4.99.16] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5cj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cj5 OCA], [~~http~~://pdbe.org/5cj5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5cj5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5cj5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5cj5 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~G7CL00_MYCTH G7CL00_MYCTH]~~] Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.[HAMAP-Rule:MF_02124]	+	[https://www.uniprot.org/uniprot/G7CL00_MYCT3 G7CL00_MYCT3] Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.[HAMAP-Rule:MF_02124]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myct3~~]]	+	[[Category: Mycolicibacterium thermoresistibile ATCC 19527]]
-	[[Category: Blaszczyk, M]]	+	[[Category: Blaszczyk M]]
-	[[Category: Blundell~~, T L~~]]	+	[[Category: Blundell TL]]
-	[[Category: Empadinhas, N]]	+	[[Category: Empadinhas N]]
-	[[Category: Maranha, A]]	+	[[Category: Maranha A]]
-	[[Category: Mendes, V]]	+	[[Category: Mendes V]]
-	~~[[Category: Gh13]]~~	+
-	~~[[Category: Glge]]~~	+
-	~~[[Category: Maltose]]~~	+
-	~~[[Category: Maltosyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:40, 27 March 2020

2020-03-27T11:40:59Z

←Older revision		Revision as of 11:40, 27 March 2020
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		+
	==Structure of Mycobacterium thermoresistibile GlgE APO form at 3.13A resolution==		==Structure of Mycobacterium thermoresistibile GlgE APO form at 3.13A resolution==
-	<StructureSection load='5cj5' size='340' side='right' caption='[[5cj5]], [[Resolution\|resolution]] 3.13Å' scene=''>	+	<StructureSection load='5cj5' size='340' side='right'caption='[[5cj5]], [[Resolution\|resolution]] 3.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5cj5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CJ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CJ5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5cj5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myct3 Myct3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CJ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CJ5 FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Starch_synthase_(maltosyl-transferring) Starch synthase (maltosyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.16 2.4.99.16] </span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glgE, KEK_12948 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1078020 MYCT3])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cj5 OCA], [http://pdbe.org/5cj5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cj5 RCSB], [http://www.ebi.ac.uk/pdbsum/5cj5 PDBsum]</span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Starch_synthase_(maltosyl-transferring) Starch synthase (maltosyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.16 2.4.99.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cj5 OCA], [http://pdbe.org/5cj5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cj5 RCSB], [http://www.ebi.ac.uk/pdbsum/5cj5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cj5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Myct3]]
	[[Category: Blaszczyk, M]]		[[Category: Blaszczyk, M]]
	[[Category: Blundell, T L]]		[[Category: Blundell, T L]]

OCA at 13:43, 9 December 2015

2015-12-09T13:43:59Z

←Older revision		Revision as of 13:43, 9 December 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of Mycobacterium thermoresistibile GlgE APO form at 3.13A resolution==
		+	<StructureSection load='5cj5' size='340' side='right' caption='[[5cj5]], [[Resolution\|resolution]] 3.13Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5cj5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CJ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CJ5 FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Starch_synthase_(maltosyl-transferring) Starch synthase (maltosyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.16 2.4.99.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cj5 OCA], [http://pdbe.org/5cj5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cj5 RCSB], [http://www.ebi.ac.uk/pdbsum/5cj5 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/G7CL00_MYCTH G7CL00_MYCTH]] Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.[HAMAP-Rule:MF_02124]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	GlgE, an enzyme of the pathway that converts trehalose to alpha-glucans, is essential for Mycobacterium tuberculosis. Inhibition of GlgE, which transfers maltose from a maltose-1-phosphate donor to alpha-glucan/maltooligosaccharide chain acceptor, leads to a toxic accumulation of maltose-1-phosphate that culminates in cellular death. Here we describe the first high-resolution mycobacterial GlgE structure from Mycobacterium thermoresistibile at 1.96 A. We show that the structure resembles that of M. tuberculosis and Streptomyces coelicolor GlgEs, reported before, with each protomer in the homodimer comprising five domains. However, in M. thermoresistibile GlgE we observe several conformational states of the S domain and provide evidence that its high flexibility is important for enzyme activity. The structures here reported shed further light on the interactions between the N-terminal domains and the catalytic domains of opposing chains and how they contribute to the catalytic reaction. Importantly this work identifies a useful surrogate system to aid the development of GlgE inhibitors against opportunistic and pathogenic mycobacteria.

-	~~The entry 5cj5 is ON HOLD until Paper Publication~~	+	Structure of Mycobacterium thermoresistibile GlgE defines novel conformational states that contribute to the catalytic mechanism.,Mendes V, Blaszczyk M, Maranha A, Empadinhas N, Blundell TL Sci Rep. 2015 Nov 30;5:17144. doi: 10.1038/srep17144. PMID:26616850<ref>PMID:26616850</ref>

-	~~Authors: Mendes~~, V.~~, Blaszczyk, M~~.~~, Maranha, A., Empadinhas, N~~., Blundell, T.L.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Structure of Mycobacterium thermoresistibile GlgE APO form at 3.13A resolution~~	+	<div class="pdbe-citations 5cj5" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Blaszczyk, M]]
		+	[[Category: Blundell, T L]]
		+	[[Category: Empadinhas, N]]
	[[Category: Maranha, A]]		[[Category: Maranha, A]]
-	[[Category: Empadinhas, N]]
-	[[Category: Blaszczyk, M]]
-	[[Category: Blundell, T.L]]
	[[Category: Mendes, V]]		[[Category: Mendes, V]]
		+	[[Category: Gh13]]
		+	[[Category: Glge]]
		+	[[Category: Maltose]]
		+	[[Category: Maltosyltransferase]]
		+	[[Category: Transferase]]

OCA: Protected "5cj5" [edit=sysop:move=sysop]

2015-07-22T13:12:17Z

Protected "5cj5" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:12, 22 July 2015

OCA: New page: '''Unreleased structure''' The entry 5cj5 is ON HOLD until Paper Publication Authors: Mendes, V., Blaszczyk, M., Maranha, A., Empadinhas, N., Blundell, T.L. Description: Structure of M...

2015-07-22T13:12:16Z

New page: '''Unreleased structure''' The entry 5cj5 is ON HOLD until Paper Publication Authors: Mendes, V., Blaszczyk, M., Maranha, A., Empadinhas, N., Blundell, T.L. Description: Structure of M...

New page

'''Unreleased structure'''

The entry 5cj5 is ON HOLD until Paper Publication

Authors: Mendes, V., Blaszczyk, M., Maranha, A., Empadinhas, N., Blundell, T.L.

Description: Structure of Mycobacterium thermoresistibile GlgE APO form at 3.13A resolution
[[Category: Unreleased Structures]]
[[Category: Maranha, A]]
[[Category: Empadinhas, N]]
[[Category: Blaszczyk, M]]
[[Category: Blundell, T.L]]
[[Category: Mendes, V]]