5cve - Revision history

OCA at 10:09, 21 June 2023

2023-06-21T10:09:26Z

←Older revision		Revision as of 10:09, 21 June 2023
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	==Crystal Structure of human NRMT1 in complex with dimethylated fly H2B peptide and SAH==		==Crystal Structure of human NRMT1 in complex with dimethylated fly H2B peptide and SAH==
-	<StructureSection load='5cve' size='340' side='right' caption='[[5cve]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='5cve' size='340' side='right'caption='[[5cve]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5cve]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CVE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5CVE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5cve]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CVE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CVE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PBE:1,1-DIMETHYL-PROLINIUM'>PBE</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	~~<tr~~ id~~='NonStdRes'><td class~~="~~sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat~~"><scene name='pdbligand=PBE:1,1-DIMETHYL-PROLINIUM'>PBE</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cve OCA], [https://pdbe.org/5cve PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cve RCSB], [https://www.ebi.ac.uk/pdbsum/5cve PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cve ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5cvd\|5cvd]]</td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NTMT1~~,~~NRMT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~<~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_N~~-~~terminal_methyltransferase Protein N~~-~~terminal methyltransferase], with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=2.1.1.244 2.1.1.244]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5cve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cve OCA], [~~http~~://pdbe.org/5cve PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5cve RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5cve PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5cve ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NTM1A_HUMAN NTM1A_HUMAN]] Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.<ref>PMID:20481588</ref> <ref>PMID:20668449</ref> <ref>PMID:24090352</ref>	+	[https://www.uniprot.org/uniprot/NTM1A_HUMAN NTM1A_HUMAN] Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.<ref>PMID:20481588</ref> <ref>PMID:20668449</ref> <ref>PMID:24090352</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Drosophila melanogaster]]
-	[[Category: ~~Protein N-terminal methyltransferase~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Li, H~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Wu, R~~]]	+	[[Category: Li H]]
-	[[Category: ~~N-terminal methyltransferase]]~~	+	[[Category: Wu R]]
-	~~[[Category: Transferase-peptide complex~~]]	+

OCA at 12:29, 6 November 2017

2017-11-06T12:29:22Z

←Older revision		Revision as of 12:29, 6 November 2017
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		+
	==Crystal Structure of human NRMT1 in complex with dimethylated fly H2B peptide and SAH==		==Crystal Structure of human NRMT1 in complex with dimethylated fly H2B peptide and SAH==
	<StructureSection load='5cve' size='340' side='right' caption='[[5cve]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='5cve' size='340' side='right' caption='[[5cve]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5cve]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CVE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5cve]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CVE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PBE:1,1-DIMETHYL-PROLINIUM'>PBE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PBE:1,1-DIMETHYL-PROLINIUM'>PBE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5cvd\|5cvd]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5cvd\|5cvd]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NTMT1,NRMT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_N-terminal_methyltransferase Protein N-terminal methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.244 2.1.1.244] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_N-terminal_methyltransferase Protein N-terminal methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.244 2.1.1.244] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cve OCA], [http://pdbe.org/5cve PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cve RCSB], [http://www.ebi.ac.uk/pdbsum/5cve PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cve OCA], [http://pdbe.org/5cve PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cve RCSB], [http://www.ebi.ac.uk/pdbsum/5cve PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cve ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Protein N-terminal methyltransferase]]		[[Category: Protein N-terminal methyltransferase]]
	[[Category: Li, H]]		[[Category: Li, H]]

OCA at 04:36, 1 December 2015

2015-12-01T04:36:25Z

←Older revision		Revision as of 04:36, 1 December 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal Structure of human NRMT1 in complex with dimethylated fly H2B peptide and SAH==
		+	<StructureSection load='5cve' size='340' side='right' caption='[[5cve]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5cve]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CVE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PBE:1,1-DIMETHYL-PROLINIUM'>PBE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5cvd\|5cvd]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_N-terminal_methyltransferase Protein N-terminal methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.244 2.1.1.244] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cve OCA], [http://pdbe.org/5cve PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cve RCSB], [http://www.ebi.ac.uk/pdbsum/5cve PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NTM1A_HUMAN NTM1A_HUMAN]] Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.<ref>PMID:20481588</ref> <ref>PMID:20668449</ref> <ref>PMID:24090352</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	NRMT1 is an N-terminal methyltransferase that methylates histone CENP-A as well as nonhistone substrates. Here, we report the crystal structure of human NRMT1 bound to CENP-A peptide at 1.3 A. NRMT1 adopts a core methyltransferase fold that resembles DOT1L and PRMT but not SET domain family histone methyltransferases. Key substrate recognition and catalytic residues were identified by mutagenesis studies. Histone peptide profiling revealed that human NRMT1 is highly selective to human CENP-A and fruit fly H2B, which share a common "Xaa-Pro-Lys/Arg" motif. These results, along with a 1.5 A costructure of human NRMT1 bound to the fruit fly H2B peptide, underscore the importance of the NRMT1 recognition motif.

-	~~The entry 5cve is ON HOLD~~	+	Molecular basis for histone N-terminal methylation by NRMT1.,Wu R, Yue Y, Zheng X, Li H Genes Dev. 2015 Nov 15;29(22):2337-42. doi: 10.1101/gad.270926.115. Epub 2015 Nov, 5. PMID:26543159<ref>PMID:26543159</ref>

-	~~Authors: Wu~~, R.~~, Li, H~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal Structure of human NRMT1 in complex with dimethylated fly H2B peptide and SAH~~	+	<div class="pdbe-citations 5cve" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Protein N-terminal methyltransferase]]
	[[Category: Li, H]]		[[Category: Li, H]]
	[[Category: Wu, R]]		[[Category: Wu, R]]
		+	[[Category: N-terminal methyltransferase]]
		+	[[Category: Transferase-peptide complex]]

OCA: Protected "5cve" [edit=sysop:move=sysop]

2015-08-05T19:00:20Z

Protected "5cve" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:00, 5 August 2015

OCA: New page: '''Unreleased structure''' The entry 5cve is ON HOLD Authors: Wu, R., Li, H. Description: Crystal Structure of human NRMT1 in complex with dimethylated fly H2B peptide and SAH [[Catego...

2015-08-05T19:00:19Z

New page: '''Unreleased structure''' The entry 5cve is ON HOLD Authors: Wu, R., Li, H. Description: Crystal Structure of human NRMT1 in complex with dimethylated fly H2B peptide and SAH [[Catego...

New page

'''Unreleased structure'''

The entry 5cve is ON HOLD

Authors: Wu, R., Li, H.

Description: Crystal Structure of human NRMT1 in complex with dimethylated fly H2B peptide and SAH
[[Category: Unreleased Structures]]
[[Category: Li, H]]
[[Category: Wu, R]]