5dil - Revision history

OCA at 21:43, 28 June 2023

2023-06-28T21:43:09Z

←Older revision		Revision as of 21:43, 28 June 2023
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	<StructureSection load='5dil' size='340' side='right'caption='[[5dil]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='5dil' size='340' side='right'caption='[[5dil]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dil]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Influenza_b_virus_~~(b/~~singapore~~/~~dso_090134~~/2004) Influenza b virus (b/~~singapore~~/~~dso_090134~~/2004)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DIL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5DIL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dil]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_B_virus_(B/Singapore/DSO_090134/2004) Influenza B virus (B/Singapore/DSO_090134/2004)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DIL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=IOD:IODIDE+ION'>IOD</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.01Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">NS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=741218 Influenza B virus (B/Singapore/DSO_090134/2004)])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dil OCA], [https://pdbe.org/5dil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dil RCSB], [https://www.ebi.ac.uk/pdbsum/5dil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dil ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5dil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dil OCA], [~~http~~://pdbe.org/5dil PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5dil RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5dil PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5dil ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/X2C382_9INFB X2C382_9INFB]] Binds and inhibits the ubiquitin-like protein G1P2/ISG15, which is an early antiviral protein. Inhibits IRF-3 nuclear translocation and activation. Inhibits IFN-beta promoter activation; this inhibition is not dsRNA-binding dependent Prevents EIF2AK2/PKR activation, either by binding double strand RNA or by interacting directly with EIF2AK2/PKR. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells.[PIRNR:PIRNR003938]	+	[https://www.uniprot.org/uniprot/X2C382_9INFB X2C382_9INFB] Binds and inhibits the ubiquitin-like protein G1P2/ISG15, which is an early antiviral protein. Inhibits IRF-3 nuclear translocation and activation. Inhibits IFN-beta promoter activation; this inhibition is not dsRNA-binding dependent Prevents EIF2AK2/PKR activation, either by binding double strand RNA or by interacting directly with EIF2AK2/PKR. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells.[PIRNR:PIRNR003938]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Guan, R]]	+	[[Category: Guan R]]
-	[[Category: Hamilton, K]]	+	[[Category: Hamilton K]]
-	[[Category: Ma, L]]	+	[[Category: Ma L]]
-	[[Category: Montelione~~, G T]]~~	+	[[Category: Montelione GT]]
-	~~[[Category: Effector domain]]~~	+
-	~~[[Category: Rna binding]]~~	+
-	~~[[Category: Rna-binding protein]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 11:24, 25 December 2019

2019-12-25T11:24:32Z

←Older revision		Revision as of 11:24, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of the effector domain of the NS1 protein from influenza virus B==		==Crystal structure of the effector domain of the NS1 protein from influenza virus B==
-	<StructureSection load='5dil' size='340' side='right' caption='[[5dil]], [[Resolution\|resolution]] 2.01Å' scene=''>	+	<StructureSection load='5dil' size='340' side='right'caption='[[5dil]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5dil]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DIL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5dil]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_b_virus_(b/singapore/dso_090134/2004) Influenza b virus (b/singapore/dso_090134/2004)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DIL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=741218 Influenza B virus (B/Singapore/DSO_090134/2004)])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dil OCA], [http://pdbe.org/5dil PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dil RCSB], [http://www.ebi.ac.uk/pdbsum/5dil PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dil ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dil OCA], [http://pdbe.org/5dil PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dil RCSB], [http://www.ebi.ac.uk/pdbsum/5dil PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dil ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Guan, R]]		[[Category: Guan, R]]
	[[Category: Hamilton, K]]		[[Category: Hamilton, K]]

OCA at 14:28, 5 October 2016

2016-10-05T14:28:42Z

←Older revision		Revision as of 14:28, 5 October 2016
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/X2C382_9INFB X2C382_9INFB]] Binds and inhibits the ubiquitin-like protein G1P2/ISG15, which is an early antiviral protein. Inhibits IRF-3 nuclear translocation and activation. Inhibits IFN-beta promoter activation; this inhibition is not dsRNA-binding dependent Prevents EIF2AK2/PKR activation, either by binding double strand RNA or by interacting directly with EIF2AK2/PKR. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells.[PIRNR:PIRNR003938]		[[http://www.uniprot.org/uniprot/X2C382_9INFB X2C382_9INFB]] Binds and inhibits the ubiquitin-like protein G1P2/ISG15, which is an early antiviral protein. Inhibits IRF-3 nuclear translocation and activation. Inhibits IFN-beta promoter activation; this inhibition is not dsRNA-binding dependent Prevents EIF2AK2/PKR activation, either by binding double strand RNA or by interacting directly with EIF2AK2/PKR. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells.[PIRNR:PIRNR003938]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Influenza viruses cause a highly contagious respiratory disease in humans. The NS1 proteins of influenza A and B viruses (NS1A and NS1B proteins, respectively) are composed of two domains, a dimeric N-terminal domain and a C-terminal domain, connected by a flexible polypeptide linker. Here we report the 2.0-A X-ray crystal structure and nuclear magnetic resonance studies of the NS1B C-terminal domain, which reveal a novel and unexpected basic RNA-binding site that is not present in the NS1A protein. We demonstrate that single-site alanine replacements of basic residues in this site lead to reduced RNA-binding activity, and that recombinant influenza B viruses expressing these mutant NS1B proteins are severely attenuated in replication. This novel RNA-binding site of NS1B is required for optimal influenza B virus replication. Most importantly, this study reveals an unexpected RNA-binding function in the C-terminal domain of NS1B, a novel function that distinguishes influenza B viruses from influenza A viruses.
		+
		+	A Second RNA-Binding Site in the NS1 Protein of Influenza B Virus.,Ma LC, Guan R, Hamilton K, Aramini JM, Mao L, Wang S, Krug RM, Montelione GT Structure. 2016 Sep 6;24(9):1562-72. doi: 10.1016/j.str.2016.07.001. Epub 2016, Aug 18. PMID:27545620<ref>PMID:27545620</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5dil" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:22, 10 August 2016

2016-08-10T16:22:32Z

←Older revision		Revision as of 16:22, 10 August 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5dil is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the effector domain of the NS1 protein from influenza virus B==
-		+	<StructureSection load='5dil' size='340' side='right' caption='[[5dil]], [[Resolution\|resolution]] 2.01Å' scene=''>
-	~~Authors~~: ~~Guan~~, R., ~~Hamilton~~, K., ~~Ma, L~~., ~~Montelione, G~~.T.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5dil]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DIL FirstGlance]. <br>
-	~~Description: Crystal structure of the effector domain of the NS1 protein from influenza virus B~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	[[Category: ~~Montelione~~, ~~G.T~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dil OCA], [http://pdbe.org/5dil PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dil RCSB], [http://www.ebi.ac.uk/pdbsum/5dil PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dil ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/X2C382_9INFB X2C382_9INFB]] Binds and inhibits the ubiquitin-like protein G1P2/ISG15, which is an early antiviral protein. Inhibits IRF-3 nuclear translocation and activation. Inhibits IFN-beta promoter activation; this inhibition is not dsRNA-binding dependent Prevents EIF2AK2/PKR activation, either by binding double strand RNA or by interacting directly with EIF2AK2/PKR. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells.[PIRNR:PIRNR003938]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Guan, R]]
	[[Category: Hamilton, K]]		[[Category: Hamilton, K]]
	[[Category: Ma, L]]		[[Category: Ma, L]]
-	[[Category: ~~Guan~~, R]]	+	[[Category: Montelione, G T]]
		+	[[Category: Effector domain]]
		+	[[Category: Rna binding]]
		+	[[Category: Rna-binding protein]]
		+	[[Category: Viral protein]]

OCA at 11:10, 16 December 2015

2015-12-16T11:10:48Z

←Older revision		Revision as of 11:10, 16 December 2015
Line 7:		Line 7:
	Description: Crystal structure of the effector domain of the NS1 protein from influenza virus B		Description: Crystal structure of the effector domain of the NS1 protein from influenza virus B
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Montelione, G.T]]
	[[Category: Hamilton, K]]		[[Category: Hamilton, K]]
	[[Category: Ma, L]]		[[Category: Ma, L]]
-	[[Category: Montelione, G.T]]
	[[Category: Guan, R]]		[[Category: Guan, R]]

OCA at 13:01, 30 September 2015

2015-09-30T13:01:53Z

←Older revision		Revision as of 13:01, 30 September 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5dil is ON HOLD	+	The entry 5dil is ON HOLD until Paper Publication

	Authors: Guan, R., Hamilton, K., Ma, L., Montelione, G.T.		Authors: Guan, R., Hamilton, K., Ma, L., Montelione, G.T.

OCA: Protected "5dil" [edit=sysop:move=sysop]

2015-09-09T10:16:02Z

Protected "5dil" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:16, 9 September 2015

OCA: New page: '''Unreleased structure''' The entry 5dil is ON HOLD Authors: Guan, R., Hamilton, K., Ma, L., Montelione, G.T. Description: Crystal structure of the effector domain of the NS1 protein ...

2015-09-09T10:16:00Z

New page: '''Unreleased structure''' The entry 5dil is ON HOLD Authors: Guan, R., Hamilton, K., Ma, L., Montelione, G.T. Description: Crystal structure of the effector domain of the NS1 protein ...

New page

'''Unreleased structure'''

The entry 5dil is ON HOLD

Authors: Guan, R., Hamilton, K., Ma, L., Montelione, G.T.

Description: Crystal structure of the effector domain of the NS1 protein from influenza virus B
[[Category: Unreleased Structures]]
[[Category: Hamilton, K]]
[[Category: Ma, L]]
[[Category: Montelione, G.T]]
[[Category: Guan, R]]