5ecj - Revision history

OCA at 12:28, 6 March 2024

2024-03-06T12:28:08Z

←Older revision		Revision as of 12:28, 6 March 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/PRD14_MOUSE PRD14_MOUSE] Transcription factor that has both positive and negative roles on transcription. Plays a role in cellular pluripotency (By similarity). Essential for germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression.<ref>PMID:18622394</ref> [https://www.uniprot.org/uniprot/MTG8R_MOUSE MTG8R_MOUSE] Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Via association with PRDM14 is involved in regulation of embryonic stem cell (ESC) pluripotency. Involved in primordial germ cell (PCG) formation (PubMed:27281218). Stabilizes PRDM14 and OCT4 on chromatin in a homooligomerization-dependent mannerCan repress the expression of MMP7 in a ZBTB33-dependent manner (By similarity). Through heteromerization with CBFA2T3/MTG16 may be involved in regulation of the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:19799863). Required for the maintenance of the secretory cell lineage in the small intestine (PubMed:16227606). Can inhibit Notch signaling probably by association with RBPJ and may be involved in GFI1-mediated Paneth cell differentiation (PubMed:25398765).[UniProtKB:O43439]<ref>PMID:16227606</ref> <ref>PMID:25398765</ref> <ref>PMID:27281218</ref>		[https://www.uniprot.org/uniprot/PRD14_MOUSE PRD14_MOUSE] Transcription factor that has both positive and negative roles on transcription. Plays a role in cellular pluripotency (By similarity). Essential for germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression.<ref>PMID:18622394</ref> [https://www.uniprot.org/uniprot/MTG8R_MOUSE MTG8R_MOUSE] Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Via association with PRDM14 is involved in regulation of embryonic stem cell (ESC) pluripotency. Involved in primordial germ cell (PCG) formation (PubMed:27281218). Stabilizes PRDM14 and OCT4 on chromatin in a homooligomerization-dependent mannerCan repress the expression of MMP7 in a ZBTB33-dependent manner (By similarity). Through heteromerization with CBFA2T3/MTG16 may be involved in regulation of the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:19799863). Required for the maintenance of the secretory cell lineage in the small intestine (PubMed:16227606). Can inhibit Notch signaling probably by association with RBPJ and may be involved in GFI1-mediated Paneth cell differentiation (PubMed:25398765).[UniProtKB:O43439]<ref>PMID:16227606</ref> <ref>PMID:25398765</ref> <ref>PMID:27281218</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Prdm14 is a sequence-specific transcriptional regulator of embryonic stem cell (ESC) pluripotency and primordial germ cell (PGC) formation. It exerts its function, at least in part, through repressing genes associated with epigenetic modification and cell differentiation. Here, we show that this repressive function is mediated through an ETO-family co-repressor Mtgr1, which tightly binds to the pre-SET/SET domains of Prdm14 and co-occupies its genomic targets in mouse ESCs. We generated two monobodies, synthetic binding proteins, targeting the Prdm14 SET domain and demonstrate their utility, respectively, in facilitating crystallization and structure determination of the Prdm14-Mtgr1 complex, or as genetically encoded inhibitor of the Prdm14-Mtgr1 interaction. Structure-guided point mutants and the monobody abrogated the Prdm14-Mtgr1 association and disrupted Prdm14's function in mESC gene expression and PGC formation in vitro. Altogether, our work uncovers the molecular mechanism underlying Prdm14-mediated repression and provides renewable reagents for studying and controlling Prdm14 functions.
-
-	ETO family protein Mtgr1 mediates Prdm14 functions in stem cell maintenance and primordial germ cell formation.,Nady N, Gupta A, Ma Z, Swigut T, Koide A, Koide S, Wysocka J Elife. 2015 Nov 2;4. pii: e10150. doi: 10.7554/eLife.10150. PMID:26523391<ref>PMID:26523391</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5ecj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:21, 5 July 2023

2023-07-05T06:21:19Z

←Older revision		Revision as of 06:21, 5 July 2023
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	<StructureSection load='5ecj' size='340' side='right'caption='[[5ecj]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='5ecj' size='340' side='right'caption='[[5ecj]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ecj]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~] and [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ECJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ecj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ECJ FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Cbfa2t2~~, ~~Cbfa2t2h, Mtgr1 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.05Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ecj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ecj OCA], [~~http~~://pdbe.org/5ecj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ecj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ecj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ecj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ecj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ecj OCA], [https://pdbe.org/5ecj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ecj RCSB], [https://www.ebi.ac.uk/pdbsum/5ecj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ecj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/MTG8R_MOUSE MTG8R_MOUSE] Required for tumorigenesis in a AOM/DSS colitis-associated carcinoma model. May be involved in intestinal tumorigenesis.<ref>PMID:21303973</ref>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PRD14_MOUSE PRD14_MOUSE]] Transcription factor that has both positive and negative roles on transcription. Plays a role in cellular pluripotency (By similarity). Essential for germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression.<ref>PMID:18622394</ref>	+	[https://www.uniprot.org/uniprot/PRD14_MOUSE PRD14_MOUSE] Transcription factor that has both positive and negative roles on transcription. Plays a role in cellular pluripotency (By similarity). Essential for germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression.<ref>PMID:18622394</ref> [https://www.uniprot.org/uniprot/MTG8R_MOUSE MTG8R_MOUSE] Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Via association with PRDM14 is involved in regulation of embryonic stem cell (ESC) pluripotency. Involved in primordial germ cell (PCG) formation (PubMed:27281218). Stabilizes PRDM14 and OCT4 on chromatin in a homooligomerization-dependent mannerCan repress the expression of MMP7 in a ZBTB33-dependent manner (By similarity). Through heteromerization with CBFA2T3/MTG16 may be involved in regulation of the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:19799863). Required for the maintenance of the secretory cell lineage in the small intestine (PubMed:16227606). Can inhibit Notch signaling probably by association with RBPJ and may be involved in GFI1-mediated Paneth cell differentiation (PubMed:25398765).[UniProtKB:O43439]<ref>PMID:16227606</ref> <ref>PMID:25398765</ref> <ref>PMID:27281218</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Gupta, A]]	+	[[Category: Gupta A]]
-	[[Category: Koide, S]]	+	[[Category: Koide S]]
-	~~[[Category: Gene regulation-transcription complex]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 16:06, 11 December 2019

2019-12-11T16:06:34Z

←Older revision		Revision as of 16:06, 11 December 2019
Line 1:		Line 1:

	==Crystal structure of monobody Mb(S4) bound to Prdm14 in complex with Mtgr1==		==Crystal structure of monobody Mb(S4) bound to Prdm14 in complex with Mtgr1==
-	<StructureSection load='5ecj' size='340' side='right' caption='[[5ecj]], [[Resolution\|resolution]] 3.05Å' scene=''>	+	<StructureSection load='5ecj' size='340' side='right'caption='[[5ecj]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ecj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ECJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ecj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ECJ FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Gupta, A]]		[[Category: Gupta, A]]

OCA at 17:03, 15 November 2017

2017-11-15T17:03:28Z

←Older revision		Revision as of 17:03, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5ecj' size='340' side='right' caption='[[5ecj]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='5ecj' size='340' side='right' caption='[[5ecj]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ecj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ECJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ecj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ECJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ecj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ecj OCA], [http://pdbe.org/5ecj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ecj RCSB], [http://www.ebi.ac.uk/pdbsum/5ecj PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cbfa2t2, Cbfa2t2h, Mtgr1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ecj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ecj OCA], [http://pdbe.org/5ecj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ecj RCSB], [http://www.ebi.ac.uk/pdbsum/5ecj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ecj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Gupta, A]]		[[Category: Gupta, A]]
	[[Category: Koide, S]]		[[Category: Koide, S]]

OCA at 19:27, 10 May 2016

2016-05-10T19:27:42Z

←Older revision		Revision as of 19:27, 10 May 2016
Line 1:		Line 1:
		+
	==Crystal structure of monobody Mb(S4) bound to Prdm14 in complex with Mtgr1==		==Crystal structure of monobody Mb(S4) bound to Prdm14 in complex with Mtgr1==
	<StructureSection load='5ecj' size='340' side='right' caption='[[5ecj]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='5ecj' size='340' side='right' caption='[[5ecj]], [[Resolution\|resolution]] 3.05Å' scene=''>

OCA at 16:10, 2 December 2015

2015-12-02T16:10:27Z

←Older revision		Revision as of 16:10, 2 December 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of monobody Mb(S4) bound to Prdm14 in complex with Mtgr1==
		+	<StructureSection load='5ecj' size='340' side='right' caption='[[5ecj]], [[Resolution\|resolution]] 3.05Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ecj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ECJ FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ecj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ecj OCA], [http://pdbe.org/5ecj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ecj RCSB], [http://www.ebi.ac.uk/pdbsum/5ecj PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PRD14_MOUSE PRD14_MOUSE]] Transcription factor that has both positive and negative roles on transcription. Plays a role in cellular pluripotency (By similarity). Essential for germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression.<ref>PMID:18622394</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Prdm14 is a sequence-specific transcriptional regulator of embryonic stem cell (ESC) pluripotency and primordial germ cell (PGC) formation. It exerts its function, at least in part, through repressing genes associated with epigenetic modification and cell differentiation. Here, we show that this repressive function is mediated through an ETO-family co-repressor Mtgr1, which tightly binds to the pre-SET/SET domains of Prdm14 and co-occupies its genomic targets in mouse ESCs. We generated two monobodies, synthetic binding proteins, targeting the Prdm14 SET domain and demonstrate their utility, respectively, in facilitating crystallization and structure determination of the Prdm14-Mtgr1 complex, or as genetically encoded inhibitor of the Prdm14-Mtgr1 interaction. Structure-guided point mutants and the monobody abrogated the Prdm14-Mtgr1 association and disrupted Prdm14's function in mESC gene expression and PGC formation in vitro. Altogether, our work uncovers the molecular mechanism underlying Prdm14-mediated repression and provides renewable reagents for studying and controlling Prdm14 functions.

-	~~The entry 5ecj is ON HOLD~~	+	ETO family protein Mtgr1 mediates Prdm14 functions in stem cell maintenance and primordial germ cell formation.,Nady N, Gupta A, Ma Z, Swigut T, Koide A, Koide S, Wysocka J Elife. 2015 Nov 2;4. pii: e10150. doi: 10.7554/eLife.10150. PMID:26523391<ref>PMID:26523391</ref>

-	~~Authors: Gupta~~, A.~~, Koide,~~ S.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~:	+	<div class="pdbe-citations 5ecj" style="background-color:#fffaf0;"></div>
-	~~[[Category: Unreleased Structures]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Gupta, A]]		[[Category: Gupta, A]]
	[[Category: Koide, S]]		[[Category: Koide, S]]
		+	[[Category: Gene regulation-transcription complex]]
		+	[[Category: Protein binding]]
		+	[[Category: Transferase]]

OCA: Protected "5ecj" [edit=sysop:move=sysop]

2015-12-01T02:38:59Z

Protected "5ecj" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:38, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5ecj is ON HOLD Authors: Gupta, A., Koide, S. Description: Category: Unreleased Structures Category: Gupta, A Category: Koide, S

2015-12-01T02:38:58Z

New page: '''Unreleased structure''' The entry 5ecj is ON HOLD Authors: Gupta, A., Koide, S. Description: Category: Unreleased Structures Category: Gupta, A Category: Koide, S

New page

'''Unreleased structure'''

The entry 5ecj is ON HOLD

Authors: Gupta, A., Koide, S.

Description:
[[Category: Unreleased Structures]]
[[Category: Gupta, A]]
[[Category: Koide, S]]