5eco - Revision history

OCA at 06:21, 5 July 2023

2023-07-05T06:21:26Z

←Older revision		Revision as of 06:21, 5 July 2023
Line 3:		Line 3:
	<StructureSection load='5eco' size='340' side='right'caption='[[5eco]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5eco' size='340' side='right'caption='[[5eco]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5eco]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5ECO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5eco]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ECO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=JAA:{(1R,2R)-3-OXO-2-[(2Z)-PENT-2-EN-1-YL]CYCLOPENTYL}ACETIC+ACID'>JAA</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ech\|5ech]], [[5eci\|5eci]], [[5eck\|5eck]], [[5ecl\|5ecl]], [[5ecm\|5ecm]], [[5ecn\|5ecn]], [[5ecp\|5ecp]], [[5ecq\|5ecq]], [[5ecr\|5ecr]], [[5ecs\|5ecs]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=JAA:{(1R,2R)-3-OXO-2-[(2Z)-PENT-2-EN-1-YL]CYCLOPENTYL}ACETIC+ACID'>JAA</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">JAR1, FIN219, At2g46370, F11C10.6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), GSTU20, FIP1, At1g78370, F3F9.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eco OCA], [https://pdbe.org/5eco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eco RCSB], [https://www.ebi.ac.uk/pdbsum/5eco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eco ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5eco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eco OCA], [~~http~~://pdbe.org/5eco PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5eco RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5eco PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5eco ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/JAR1_ARATH JAR1_ARATH]] Catalyzes the synthesis of jasmonates-amino acid conjugates by adenylation; can use Ile and, in vitro at least, Val, Leu and Phe as conjugating amino acids on jasmonic acid (JA) and 9,10-dihydro-JA substrates, and to a lower extent, on 3-oxo-2-(2Z-pentenyl)-cyclopentane-1-butyric acid (OPC-4) and 12-hydroxy-JA (12-OH-JA). Can synthesize adenosine 5-tetraphosphate in vitro. Required for the JA-mediated signaling pathway that regulates many developmental and defense mechanisms, including growth root inhibition, vegetative storage proteins (VSPs) accumulation, induced systemic resistance (ISR), response to wounding and herbivores, tolerance to ozone O(3) (probably having a role in lesion containment). Plays an important role in the accumulation of JA-Ile in response to wounding, both locally and systemically; promotes JA responding genes especially in distal part of wounded plants, via the JA-Ile-stimulated degradation of JAZ repressor proteins by the SCF(COI)E3 ubiquitin-protein ligase pathway. Involved in the apoptosis-like programmed cell death (PCD) induced by fungal toxin fumonisin B1-mediated (FB1). Required for volatile compounds (C6-aldehydes and allo-ocimene)-mediated defense activation. Involved in the non-pathogenic rhizobacterium-mediated ISR (defense priming) by P.fluorescens (strains CHAOr and WCS417r) and P.putida LSW17S against infection leaf pathogens such as P.syringae pv. tomato and H.parasitica. Required for the JA-dependent resistance to fungi such as P.irregulare, U.vignae and U.appendiculatus. Necessary to induce systemic resistance against R.solanaceraum and P.syringae pv. tomato with P.oligandrum (a non-pathogenic biocontrol agent) cell wall protein fraction (CWP). Mediates PGIP2 accumulation in response to B.cinerea infection and thus contributes to resistance against this pathogen. Modulates the UV-B alteration of leaves attractiveness to diamondback moths P.xylostella leading to insect oviposition. Involved in the regulation of far-red light influence on development. Seems necessary for the salicylic acid (SA)-mediated, NPR1-independent resistance pathway. May contribute to the chitin-elicited pathway. Contributes to the sensitivity toward F.graminearum.<ref>PMID:10921909</ref> <ref>PMID:11006337</ref> <ref>PMID:11041879</ref> <ref>PMID:11041881</ref> <ref>PMID:11090217</ref> <ref>PMID:11607311</ref> <ref>PMID:12084835</ref> <ref>PMID:12236603</ref> <ref>PMID:12376653</ref> <ref>PMID:12509524</ref> <ref>PMID:12744510</ref> <ref>PMID:12848825</ref> <ref>PMID:14558686</ref> <ref>PMID:15258265</ref> <ref>PMID:15879447</ref> <ref>PMID:16639567</ref> <ref>PMID:17220357</ref> <ref>PMID:17291501</ref> <ref>PMID:17601164</ref> <ref>PMID:18247047</ref> <ref>PMID:19251652</ref> <ref>PMID:19304739</ref> <ref>PMID:19473329</ref> <ref>PMID:20521949</ref> <ref>PMID:9724702</ref> <ref>PMID:9807813</ref> [[http://www.uniprot.org/uniprot/GSTUK_ARATH GSTUK_ARATH]] Exhibits glutathione-dependent thiol transferase activities. Can use glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates. Involved in the regulation of far-red light influence on development.<ref>PMID:17220357</ref>	+	[https://www.uniprot.org/uniprot/JAR1_ARATH JAR1_ARATH] Catalyzes the synthesis of jasmonates-amino acid conjugates by adenylation; can use Ile and, in vitro at least, Val, Leu and Phe as conjugating amino acids on jasmonic acid (JA) and 9,10-dihydro-JA substrates, and to a lower extent, on 3-oxo-2-(2Z-pentenyl)-cyclopentane-1-butyric acid (OPC-4) and 12-hydroxy-JA (12-OH-JA). Can synthesize adenosine 5-tetraphosphate in vitro. Required for the JA-mediated signaling pathway that regulates many developmental and defense mechanisms, including growth root inhibition, vegetative storage proteins (VSPs) accumulation, induced systemic resistance (ISR), response to wounding and herbivores, tolerance to ozone O(3) (probably having a role in lesion containment). Plays an important role in the accumulation of JA-Ile in response to wounding, both locally and systemically; promotes JA responding genes especially in distal part of wounded plants, via the JA-Ile-stimulated degradation of JAZ repressor proteins by the SCF(COI)E3 ubiquitin-protein ligase pathway. Involved in the apoptosis-like programmed cell death (PCD) induced by fungal toxin fumonisin B1-mediated (FB1). Required for volatile compounds (C6-aldehydes and allo-ocimene)-mediated defense activation. Involved in the non-pathogenic rhizobacterium-mediated ISR (defense priming) by P.fluorescens (strains CHAOr and WCS417r) and P.putida LSW17S against infection leaf pathogens such as P.syringae pv. tomato and H.parasitica. Required for the JA-dependent resistance to fungi such as P.irregulare, U.vignae and U.appendiculatus. Necessary to induce systemic resistance against R.solanaceraum and P.syringae pv. tomato with P.oligandrum (a non-pathogenic biocontrol agent) cell wall protein fraction (CWP). Mediates PGIP2 accumulation in response to B.cinerea infection and thus contributes to resistance against this pathogen. Modulates the UV-B alteration of leaves attractiveness to diamondback moths P.xylostella leading to insect oviposition. Involved in the regulation of far-red light influence on development. Seems necessary for the salicylic acid (SA)-mediated, NPR1-independent resistance pathway. May contribute to the chitin-elicited pathway. Contributes to the sensitivity toward F.graminearum.<ref>PMID:10921909</ref> <ref>PMID:11006337</ref> <ref>PMID:11041879</ref> <ref>PMID:11041881</ref> <ref>PMID:11090217</ref> <ref>PMID:11607311</ref> <ref>PMID:12084835</ref> <ref>PMID:12236603</ref> <ref>PMID:12376653</ref> <ref>PMID:12509524</ref> <ref>PMID:12744510</ref> <ref>PMID:12848825</ref> <ref>PMID:14558686</ref> <ref>PMID:15258265</ref> <ref>PMID:15879447</ref> <ref>PMID:16639567</ref> <ref>PMID:17220357</ref> <ref>PMID:17291501</ref> <ref>PMID:17601164</ref> <ref>PMID:18247047</ref> <ref>PMID:19251652</ref> <ref>PMID:19304739</ref> <ref>PMID:19473329</ref> <ref>PMID:20521949</ref> <ref>PMID:9724702</ref> <ref>PMID:9807813</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath]]~~	+	[[Category: Arabidopsis thaliana]]
-	~~[[Category: Glutathione transferase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen~~, C Y~~]]	+	[[Category: Chen CY]]
-	[[Category: Cheng~~, Y S]]~~	+	[[Category: Cheng YS]]
-	~~[[Category: Glutathione s-transferase]]~~	+
-	~~[[Category: Jasmonate-amido synthetase]]~~	+
-	~~[[Category: Ligase-transferase complex~~]]	+

OCA at 06:53, 22 April 2020

2020-04-22T06:53:43Z

←Older revision		Revision as of 06:53, 22 April 2020
Line 1:		Line 1:

	==Crystal Structure of FIN219-FIP1 complex with JA, Leu and Mg==		==Crystal Structure of FIN219-FIP1 complex with JA, Leu and Mg==
-	<StructureSection load='5eco' size='340' side='right' caption='[[5eco]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5eco' size='340' side='right'caption='[[5eco]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5eco]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ECO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5eco]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ECO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=JAA:{(1R,2R)-3-OXO-2-[(2Z)-PENT-2-EN-1-YL]CYCLOPENTYL}ACETIC+ACID'>JAA</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=JAA:{(1R,2R)-3-OXO-2-[(2Z)-PENT-2-EN-1-YL]CYCLOPENTYL}ACETIC+ACID'>JAA</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ech\|5ech]], [[5eci\|5eci]], [[5eck\|5eck]], [[5ecl\|5ecl]], [[5ecm\|5ecm]], [[5ecn\|5ecn]], [[5ecp\|5ecp]], [[5ecq\|5ecq]], [[5ecr\|5ecr]], [[5ecs\|5ecs]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ech\|5ech]], [[5eci\|5eci]], [[5eck\|5eck]], [[5ecl\|5ecl]], [[5ecm\|5ecm]], [[5ecn\|5ecn]], [[5ecp\|5ecp]], [[5ecq\|5ecq]], [[5ecr\|5ecr]], [[5ecs\|5ecs]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">JAR1, FIN219, At2g46370, F11C10.6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), GSTU20, FIP1, At1g78370, F3F9.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5eco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eco OCA], [http://pdbe.org/5eco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eco RCSB], [http://www.ebi.ac.uk/pdbsum/5eco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eco ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5eco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eco OCA], [http://pdbe.org/5eco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eco RCSB], [http://www.ebi.ac.uk/pdbsum/5eco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eco ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5eco" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5eco" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
		+	[[Category: Large Structures]]
	[[Category: Chen, C Y]]		[[Category: Chen, C Y]]
	[[Category: Cheng, Y S]]		[[Category: Cheng, Y S]]

OCA at 06:46, 9 March 2017

2017-03-09T06:46:18Z

←Older revision		Revision as of 06:46, 9 March 2017
Line 13:		Line 13:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	~~Acyl acid amido synthetases~~ of the GH3 family ~~act as critical prereceptor modulators~~ of ~~plant hormone action; however,~~ the ~~molecular basis for their hormone selectivity is unclear~~. ~~Here, we report the crystal structures~~ of ~~benzoate~~-~~specific Arabidopsis thaliana AtGH3.12/PBS3~~ and ~~jasmonic acid~~ (JA)~~-specific AtGH3~~.~~11/JAR1~~. ~~These structures~~, ~~combined with biochemical analysis~~, ~~define features for~~ the ~~conjugation~~ of ~~amino acids to diverse acyl acid~~ substrates and ~~highlight the importance~~ of ~~conformational changes in~~ the C-terminal domain ~~for catalysis. We also identify residues forming the acyl acid~~ binding ~~site across the GH3 family~~ and ~~residues critical for amino acid recognition~~. ~~Our results~~ demonstrate ~~how a highly adaptable three~~-~~dimensional scaffold~~ is ~~used~~ for the ~~evolution~~ of ~~promiscuous~~ activity ~~across an enzyme family~~ for ~~modulation of plant~~ signaling ~~molecules~~.	+	Far-red (FR) light-coupled jasmonate (JA) signaling is necessary for plant defense and development. FR insensitive 219 (FIN219) is a member of the Gretchen Hagen 3 (GH3) family of proteins in Arabidopsis and belongs to the adenylate-forming family of enzymes. It directly controls biosynthesis of jasmonoyl-isoleucine in JA-mediated defense responses and interacts with FIN219-interacting protein 1 (FIP1) under FR light conditions. FIN219 and FIP1 are involved in FR light signaling and are regulators of the interplay between light and JA signaling. However, how their interactions affect plant physiological functions remains unclear. Here, we demonstrate the crystal structures of FIN219-FIP1 while binding with substrates at atomic resolution. Our results show an unexpected FIN219 conformation and demonstrate various differences between this protein and other members of the GH3 family. We show that the rotated C-terminal domain of FIN219 alters ATP binding and the core structure of the active site. We further demonstrate that this unique FIN219-FIP1 structure is crucial for increasing FIN219 activity and determines the priority of substrate binding. We suggest that the increased FIN219 activity resulting from the complex form, a conformation for domain switching, allows FIN219 to switch to its high-affinity mode and thereby enhances JA signaling under continuous FR light conditions.

-	Structural ~~Basis for Prereceptor Modulation~~ of ~~Plant Hormones by GH3 Proteins~~.,~~Westfall CS~~, ~~Zubieta C~~, ~~Herrmann J~~, ~~Kapp~~ U, ~~Nanao MH, Jez JM Science~~. ~~2012 May 24~~. PMID:~~22628555~~<ref>PMID:~~22628555~~</ref>	+	Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation.,Chen CY, Ho SS, Kuo TY, Hsieh HL, Cheng YS Proc Natl Acad Sci U S A. 2017 Feb 21. pii: 201609980. doi:, 10.1073/pnas.1609980114. PMID:28223489<ref>PMID:28223489</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 18:17, 2 November 2016

2016-11-02T18:17:05Z

←Older revision		Revision as of 18:17, 2 November 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5eco is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal Structure of FIN219-FIP1 complex with JA, Leu and Mg==
		+	<StructureSection load='5eco' size='340' side='right' caption='[[5eco]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5eco]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ECO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=JAA:{(1R,2R)-3-OXO-2-[(2Z)-PENT-2-EN-1-YL]CYCLOPENTYL}ACETIC+ACID'>JAA</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ech\|5ech]], [[5eci\|5eci]], [[5eck\|5eck]], [[5ecl\|5ecl]], [[5ecm\|5ecm]], [[5ecn\|5ecn]], [[5ecp\|5ecp]], [[5ecq\|5ecq]], [[5ecr\|5ecr]], [[5ecs\|5ecs]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eco OCA], [http://pdbe.org/5eco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eco RCSB], [http://www.ebi.ac.uk/pdbsum/5eco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eco ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/JAR1_ARATH JAR1_ARATH]] Catalyzes the synthesis of jasmonates-amino acid conjugates by adenylation; can use Ile and, in vitro at least, Val, Leu and Phe as conjugating amino acids on jasmonic acid (JA) and 9,10-dihydro-JA substrates, and to a lower extent, on 3-oxo-2-(2Z-pentenyl)-cyclopentane-1-butyric acid (OPC-4) and 12-hydroxy-JA (12-OH-JA). Can synthesize adenosine 5-tetraphosphate in vitro. Required for the JA-mediated signaling pathway that regulates many developmental and defense mechanisms, including growth root inhibition, vegetative storage proteins (VSPs) accumulation, induced systemic resistance (ISR), response to wounding and herbivores, tolerance to ozone O(3) (probably having a role in lesion containment). Plays an important role in the accumulation of JA-Ile in response to wounding, both locally and systemically; promotes JA responding genes especially in distal part of wounded plants, via the JA-Ile-stimulated degradation of JAZ repressor proteins by the SCF(COI)E3 ubiquitin-protein ligase pathway. Involved in the apoptosis-like programmed cell death (PCD) induced by fungal toxin fumonisin B1-mediated (FB1). Required for volatile compounds (C6-aldehydes and allo-ocimene)-mediated defense activation. Involved in the non-pathogenic rhizobacterium-mediated ISR (defense priming) by P.fluorescens (strains CHAOr and WCS417r) and P.putida LSW17S against infection leaf pathogens such as P.syringae pv. tomato and H.parasitica. Required for the JA-dependent resistance to fungi such as P.irregulare, U.vignae and U.appendiculatus. Necessary to induce systemic resistance against R.solanaceraum and P.syringae pv. tomato with P.oligandrum (a non-pathogenic biocontrol agent) cell wall protein fraction (CWP). Mediates PGIP2 accumulation in response to B.cinerea infection and thus contributes to resistance against this pathogen. Modulates the UV-B alteration of leaves attractiveness to diamondback moths P.xylostella leading to insect oviposition. Involved in the regulation of far-red light influence on development. Seems necessary for the salicylic acid (SA)-mediated, NPR1-independent resistance pathway. May contribute to the chitin-elicited pathway. Contributes to the sensitivity toward F.graminearum.<ref>PMID:10921909</ref> <ref>PMID:11006337</ref> <ref>PMID:11041879</ref> <ref>PMID:11041881</ref> <ref>PMID:11090217</ref> <ref>PMID:11607311</ref> <ref>PMID:12084835</ref> <ref>PMID:12236603</ref> <ref>PMID:12376653</ref> <ref>PMID:12509524</ref> <ref>PMID:12744510</ref> <ref>PMID:12848825</ref> <ref>PMID:14558686</ref> <ref>PMID:15258265</ref> <ref>PMID:15879447</ref> <ref>PMID:16639567</ref> <ref>PMID:17220357</ref> <ref>PMID:17291501</ref> <ref>PMID:17601164</ref> <ref>PMID:18247047</ref> <ref>PMID:19251652</ref> <ref>PMID:19304739</ref> <ref>PMID:19473329</ref> <ref>PMID:20521949</ref> <ref>PMID:9724702</ref> <ref>PMID:9807813</ref> [[http://www.uniprot.org/uniprot/GSTUK_ARATH GSTUK_ARATH]] Exhibits glutathione-dependent thiol transferase activities. Can use glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates. Involved in the regulation of far-red light influence on development.<ref>PMID:17220357</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acyl acid amido synthetases of the GH3 family act as critical prereceptor modulators of plant hormone action; however, the molecular basis for their hormone selectivity is unclear. Here, we report the crystal structures of benzoate-specific Arabidopsis thaliana AtGH3.12/PBS3 and jasmonic acid (JA)-specific AtGH3.11/JAR1. These structures, combined with biochemical analysis, define features for the conjugation of amino acids to diverse acyl acid substrates and highlight the importance of conformational changes in the C-terminal domain for catalysis. We also identify residues forming the acyl acid binding site across the GH3 family and residues critical for amino acid recognition. Our results demonstrate how a highly adaptable three-dimensional scaffold is used for the evolution of promiscuous activity across an enzyme family for modulation of plant signaling molecules.

-	~~Authors: Chen~~, C~~.Y.~~, ~~Cheng~~, Y.S.	+	Structural Basis for Prereceptor Modulation of Plant Hormones by GH3 Proteins.,Westfall CS, Zubieta C, Herrmann J, Kapp U, Nanao MH, Jez JM Science. 2012 May 24. PMID:22628555<ref>PMID:22628555</ref>

-	~~Description: Crystal Structure~~ of ~~FIN219~~-~~FIP1 complex with JA, Leu and Mg~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Cheng~~, Y.S]]	+	<div class="pdbe-citations 5eco" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Chen~~, C.Y]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Glutathione transferase]]
		+	[[Category: Chen, C Y]]
		+	[[Category: Cheng, Y S]]
		+	[[Category: Glutathione s-transferase]]
		+	[[Category: Jasmonate-amido synthetase]]
		+	[[Category: Ligase-transferase complex]]

OCA at 22:14, 10 May 2016

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-	The entry 5eco is ON HOLD	+	The entry 5eco is ON HOLD until Paper Publication

	Authors: Chen, C.Y., Cheng, Y.S.		Authors: Chen, C.Y., Cheng, Y.S.

OCA: Protected "5eco" [edit=sysop:move=sysop]

2015-12-01T02:39:12Z

Protected "5eco" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:39, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5eco is ON HOLD Authors: Chen, C.Y., Cheng, Y.S. Description: Crystal Structure of FIN219-FIP1 complex with JA, Leu and Mg [[Category: Unreleased S...

2015-12-01T02:39:11Z

New page: '''Unreleased structure''' The entry 5eco is ON HOLD Authors: Chen, C.Y., Cheng, Y.S. Description: Crystal Structure of FIN219-FIP1 complex with JA, Leu and Mg [[Category: Unreleased S...

New page

'''Unreleased structure'''

The entry 5eco is ON HOLD

Authors: Chen, C.Y., Cheng, Y.S.

Description: Crystal Structure of FIN219-FIP1 complex with JA, Leu and Mg
[[Category: Unreleased Structures]]
[[Category: Cheng, Y.S]]
[[Category: Chen, C.Y]]