5ed6 - Revision history

OCA at 06:21, 5 July 2023

2023-07-05T06:21:51Z

←Older revision		Revision as of 06:21, 5 July 2023
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	<StructureSection load='5ed6' size='340' side='right'caption='[[5ed6]], [[Resolution\|resolution]] 1.52Å' scene=''>		<StructureSection load='5ed6' size='340' side='right'caption='[[5ed6]], [[Resolution\|resolution]] 1.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ed6]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ED6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5ED6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ed6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ED6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ED6 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>~~, ~~<scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-~~1~~-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.52Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5ecb\|5ecb]], [[5ecz\|5ecz]], [[5ed3\|5ed3]], [[5ed5\|5ed5]], [[5ede\|5ede]], [[5efl\|5efl]], [[5efp\|5efp]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">HINT1, HINT, PKCI1, PRKCNH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ed6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ed6 OCA], [https://pdbe.org/5ed6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ed6 RCSB], [https://www.ebi.ac.uk/pdbsum/5ed6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ed6 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ed6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ed6 OCA], [~~http~~://pdbe.org/5ed6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ed6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ed6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ed6 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HINT1_HUMAN HINT1_HUMAN]] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).	+	[https://www.uniprot.org/uniprot/HINT1_HUMAN HINT1_HUMAN] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fang, P]]	+	[[Category: Fang P]]
-	[[Category: Guo, M]]	+	[[Category: Guo M]]
-	[[Category: Wang, J]]	+	[[Category: Wang J]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Nucleotide binding]]~~	+
-	~~[[Category: Regulation of transcription]]~~	+
-	~~[[Category: Signal transduction~~]]	+

OCA at 07:07, 8 April 2020

2020-04-08T07:07:30Z

←Older revision		Revision as of 07:07, 8 April 2020
Line 3:		Line 3:
	<StructureSection load='5ed6' size='340' side='right'caption='[[5ed6]], [[Resolution\|resolution]] 1.52Å' scene=''>		<StructureSection load='5ed6' size='340' side='right'caption='[[5ed6]], [[Resolution\|resolution]] 1.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ed6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ED6 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ED6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ed6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ED6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ED6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ecb\|5ecb]], [[5ecz\|5ecz]], [[5ed3\|5ed3]], [[5ed5\|5ed5]], [[5ede\|5ede]], [[5efl\|5efl]], [[5efp\|5efp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ecb\|5ecb]], [[5ecz\|5ecz]], [[5ed3\|5ed3]], [[5ed5\|5ed5]], [[5ede\|5ede]], [[5efl\|5efl]], [[5efp\|5efp]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HINT1, HINT, PKCI1, PRKCNH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HINT1, HINT, PKCI1, PRKCNH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ed6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ed6 OCA], [http://pdbe.org/5ed6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ed6 RCSB], [http://www.ebi.ac.uk/pdbsum/5ed6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ed6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ed6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ed6 OCA], [http://pdbe.org/5ed6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ed6 RCSB], [http://www.ebi.ac.uk/pdbsum/5ed6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ed6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/HINT1_HUMAN HINT1_HUMAN]] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).		[[http://www.uniprot.org/uniprot/HINT1_HUMAN HINT1_HUMAN]] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Signal transduction systems enable organisms to monitor their external environments and accordingly adjust the cellular processes. In mast cells, the second messenger Ap4A binds to the histidine triad nucleotide-binding protein 1 (HINT1), disrupts its interaction with the microphthalmia-associated transcription factor (MITF), and eventually activates the transcription of genes downstream of MITF in response to immunostimulation. How the HINT1 protein recognizes and is regulated by Ap4A remain unclear. Here, using eight crystal structures, biochemical experiments, negative stain electron microscopy, and cellular experiments, we report that Ap4A specifically polymerizes HINT1 in solution and in activated rat basophilic leukemia cells. The polymerization interface overlaps with the area on HINT1 for MITF interaction, suggesting a possible competitive mechanism to release MITF for transcriptional activation. The mechanism depends precisely on the length of the phosphodiester linkage of Ap4A. These results highlight a direct polymerization signaling mechanism by the second messenger.
		+
		+	Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcepsilonRI-activated mast cells.,Yu J, Liu Z, Liang Y, Luo F, Zhang J, Tian C, Motzik A, Zheng M, Kang J, Zhong G, Liu C, Fang P, Guo M, Razin E, Wang J Nat Commun. 2019 Oct 11;10(1):4664. doi: 10.1038/s41467-019-12710-8. PMID:31604935<ref>PMID:31604935</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ed6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Histidine triad nucleotide-binding protein 3D structures\|Histidine triad nucleotide-binding protein 3D structures]]		*[[Histidine triad nucleotide-binding protein 3D structures\|Histidine triad nucleotide-binding protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:32, 25 December 2019

2019-12-25T11:32:12Z

←Older revision		Revision as of 11:32, 25 December 2019
Line 11:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/HINT1_HUMAN HINT1_HUMAN]] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).		[[http://www.uniprot.org/uniprot/HINT1_HUMAN HINT1_HUMAN]] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).
		+
		+	==See Also==
		+	*[[Histidine triad nucleotide-binding protein 3D structures\|Histidine triad nucleotide-binding protein 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:21, 17 April 2019

2019-04-17T06:21:04Z

←Older revision		Revision as of 06:21, 17 April 2019
Line 1:		Line 1:

	==crystal structure of human Hint1 H114A mutant complexing with ATP==		==crystal structure of human Hint1 H114A mutant complexing with ATP==
-	<StructureSection load='5ed6' size='340' side='right' caption='[[5ed6]], [[Resolution\|resolution]] 1.52Å' scene=''>	+	<StructureSection load='5ed6' size='340' side='right'caption='[[5ed6]], [[Resolution\|resolution]] 1.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ed6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ED6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ED6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ed6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ED6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ED6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ecb\|5ecb]], [[5ecz\|5ecz]], [[5ed3\|5ed3]], [[5ed5\|5ed5]], [[5ede\|5ede]], [[5efl\|5efl]], [[5efp\|5efp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ecb\|5ecb]], [[5ecz\|5ecz]], [[5ed3\|5ed3]], [[5ed5\|5ed5]], [[5ede\|5ede]], [[5efl\|5efl]], [[5efp\|5efp]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HINT1, HINT, PKCI1, PRKCNH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ed6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ed6 OCA], [http://pdbe.org/5ed6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ed6 RCSB], [http://www.ebi.ac.uk/pdbsum/5ed6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ed6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ed6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ed6 OCA], [http://pdbe.org/5ed6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ed6 RCSB], [http://www.ebi.ac.uk/pdbsum/5ed6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ed6 ProSAT]</span></td></tr>
	</table>		</table>
Line 12:		Line 13:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Fang, P]]		[[Category: Fang, P]]
	[[Category: Guo, M]]		[[Category: Guo, M]]

OCA at 23:38, 25 January 2017

2017-01-25T23:38:43Z

←Older revision		Revision as of 23:38, 25 January 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ed6 is ~~ON HOLD until Paper Publication~~	+	==crystal structure of human Hint1 H114A mutant complexing with ATP==
-		+	<StructureSection load='5ed6' size='340' side='right' caption='[[5ed6]], [[Resolution\|resolution]] 1.52Å' scene=''>
-	~~Authors~~: ~~Wang~~, J., ~~Fang~~, P., ~~Guo~~, M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ed6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ED6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ED6 FirstGlance]. <br>
-	~~Description~~: ~~crystal structure of human Hint1 H114A mutant complexing with ATP~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ecb\|5ecb]], [[5ecz\|5ecz]], [[5ed3\|5ed3]], [[5ed5\|5ed5]], [[5ede\|5ede]], [[5efl\|5efl]], [[5efp\|5efp]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ed6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ed6 OCA], [http://pdbe.org/5ed6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ed6 RCSB], [http://www.ebi.ac.uk/pdbsum/5ed6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ed6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HINT1_HUMAN HINT1_HUMAN]] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fang, P]]
	[[Category: Guo, M]]		[[Category: Guo, M]]
-	[[Category: Fang, P]]
	[[Category: Wang, J]]		[[Category: Wang, J]]
		+	[[Category: Hydrolase]]
		+	[[Category: Nucleotide binding]]
		+	[[Category: Regulation of transcription]]
		+	[[Category: Signal transduction]]

OCA: Protected "5ed6" [edit=sysop:move=sysop]

2015-12-01T02:40:11Z

Protected "5ed6" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:40, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5ed6 is ON HOLD until Paper Publication Authors: Wang, J., Fang, P., Guo, M. Description: crystal structure of human Hint1 H114A mutant complexing ...

2015-12-01T02:40:10Z

New page: '''Unreleased structure''' The entry 5ed6 is ON HOLD until Paper Publication Authors: Wang, J., Fang, P., Guo, M. Description: crystal structure of human Hint1 H114A mutant complexing ...

New page

'''Unreleased structure'''

The entry 5ed6 is ON HOLD until Paper Publication

Authors: Wang, J., Fang, P., Guo, M.

Description: crystal structure of human Hint1 H114A mutant complexing with ATP
[[Category: Unreleased Structures]]
[[Category: Guo, M]]
[[Category: Fang, P]]
[[Category: Wang, J]]