5egw - Revision history

OCA at 06:26, 5 July 2023

2023-07-05T06:26:34Z

←Older revision		Revision as of 06:26, 5 July 2023
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	<StructureSection load='5egw' size='340' side='right'caption='[[5egw]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5egw' size='340' side='right'caption='[[5egw]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5egw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ambar Ambar~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EGW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EGW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5egw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ambrosia_artemisiifolia Ambrosia artemisiifolia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EGW FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">[[~~5ef4~~\|~~5ef4~~]]</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5egw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5egw OCA], [~~http~~://pdbe.org/5egw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5egw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5egw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5egw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5egw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5egw OCA], [https://pdbe.org/5egw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5egw RCSB], [https://www.ebi.ac.uk/pdbsum/5egw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5egw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CEP01_AMBAR CEP01_AMBAR] Cysteine protease. Hydrolyzes casein and synthetic peptide Boc-Val-Leu-Lys-7-amino-4-methylcoumarin (Boc-VLK-AMC) in vitro.<ref>PMID:25865353</ref> <ref>PMID:27129273</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ambar~~]]	+	[[Category: Ambrosia artemisiifolia]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Briozzo, P]]	+	[[Category: Briozzo P]]
-	[[Category: Kopecny, D]]	+	[[Category: Kopecny D]]
-	~~[[Category: Allergen]]~~	+
-	~~[[Category: Cysteine protease]]~~	+
-	~~[[Category: Propeptide]]~~	+
-	~~[[Category: Ragweed~~]]	+

OCA at 06:57, 22 April 2020

2020-04-22T06:57:41Z

←Older revision		Revision as of 06:57, 22 April 2020
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	==2.70 A crystal structure of the Amb a 11 cysteine protease, a major ragweed pollen allergen, in its proform==		==2.70 A crystal structure of the Amb a 11 cysteine protease, a major ragweed pollen allergen, in its proform==
-	<StructureSection load='5egw' size='340' side='right' caption='[[5egw]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='5egw' size='340' side='right'caption='[[5egw]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5egw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EGW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EGW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5egw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ambar Ambar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EGW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EGW FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ef4\|5ef4]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ef4\|5ef4]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5egw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5egw OCA], [http://pdbe.org/5egw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5egw RCSB], [http://www.ebi.ac.uk/pdbsum/5egw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5egw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5egw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5egw OCA], [http://pdbe.org/5egw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5egw RCSB], [http://www.ebi.ac.uk/pdbsum/5egw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5egw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ambar]]
		+	[[Category: Large Structures]]
	[[Category: Briozzo, P]]		[[Category: Briozzo, P]]
	[[Category: Kopecny, D]]		[[Category: Kopecny, D]]

OCA at 17:53, 12 July 2016

2016-07-12T17:53:36Z

←Older revision		Revision as of 17:53, 12 July 2016
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	<table><tr><td colspan='2'>[[5egw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EGW FirstGlance]. <br>		<table><tr><td colspan='2'>[[5egw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EGW FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ef4\|5ef4]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ef4\|5ef4]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5egw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5egw OCA], [http://pdbe.org/5egw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5egw RCSB], [http://www.ebi.ac.uk/pdbsum/5egw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5egw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5egw OCA], [http://pdbe.org/5egw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5egw RCSB], [http://www.ebi.ac.uk/pdbsum/5egw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5egw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:09, 11 May 2016

2016-05-11T05:09:53Z

←Older revision		Revision as of 05:09, 11 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5egw is ~~ON HOLD until Paper~~ Publication	+	==2.70 A crystal structure of the Amb a 11 cysteine protease, a major ragweed pollen allergen, in its proform==
		+	<StructureSection load='5egw' size='340' side='right' caption='[[5egw]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5egw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EGW FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ef4\|5ef4]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5egw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5egw OCA], [http://pdbe.org/5egw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5egw RCSB], [http://www.ebi.ac.uk/pdbsum/5egw PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Allergy to the short ragweed (Ambrosia artemisiifolia) pollen is a major health problem. The ragweed allergen repertoire has been recently expanded with the identification of Amb a 11, a new major allergen belonging to the cysteine protease family. To better characterize Amb a 11, a recombinant proform of the molecule with a preserved active site was produced in Escherichia coli, refolded and processed in vitro into a mature enzyme. The enzymatic activity is revealed by maturation, following an autocatalytic processing resulting in the cleavage of both N- and C-terminal propeptides. The 2.05 A resolution crystal structure of pro-Amb a 11 shows an overall typical C1A cysteine protease fold, with a network of molecular interactions between the N-terminal propeptide and the catalytic triad of the enzyme. The allergenicity of Amb a 11 was confirmed in a murine sensitization model, resulting in airway inflammation, production of serum IgEs as well as induction of Th2 immune responses. Of note, inflammatory responses were higher with the mature form, demonstrating that the cysteine protease activity critically contributes to the allergenicity of the molecule. Collectively, our results clearly demonstrate that Amb a 11 is a bona fide cysteine protease exhibiting a strong allergenicity. As such, it should be considered as an important molecule for diagnosis and immunotherapy of ragweed pollen allergy.

-	~~Authors: Briozzo, P~~., ~~Kopecny~~, D.	+	Structural and Functional Characterization of the Major Allergen Amb a 11 from Short Ragweed Pollen.,Groeme R, Airouche S, Kopecny D, Jaekel J, Savko M, Berjont N, Bussieres L, Le Mignon M, Jagic F, Zieglmayer P, Baron-Bodo V, Bordas-Le Floch V, Mascarell L, Briozzo P, Moingeon P J Biol Chem. 2016 Apr 19. pii: jbc.M115.702001. PMID:27129273<ref>PMID:27129273</ref>

-	~~Description: 2.70 A crystal structure of the Amb a 11 cysteine protease~~, a ~~major ragweed pollen allergen, in its proform~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5egw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Briozzo, P]]		[[Category: Briozzo, P]]
	[[Category: Kopecny, D]]		[[Category: Kopecny, D]]
		+	[[Category: Allergen]]
		+	[[Category: Cysteine protease]]
		+	[[Category: Propeptide]]
		+	[[Category: Ragweed]]

OCA: Protected "5egw" [edit=sysop:move=sysop]

2015-12-01T02:49:00Z

Protected "5egw" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:49, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5egw is ON HOLD until Paper Publication Authors: Briozzo, P., Kopecny, D. Description: 2.70 A crystal structure of the Amb a 11 cysteine protease, ...

2015-12-01T02:48:59Z

New page: '''Unreleased structure''' The entry 5egw is ON HOLD until Paper Publication Authors: Briozzo, P., Kopecny, D. Description: 2.70 A crystal structure of the Amb a 11 cysteine protease, ...

New page

'''Unreleased structure'''

The entry 5egw is ON HOLD until Paper Publication

Authors: Briozzo, P., Kopecny, D.

Description: 2.70 A crystal structure of the Amb a 11 cysteine protease, a major ragweed pollen allergen, in its proform
[[Category: Unreleased Structures]]
[[Category: Briozzo, P]]
[[Category: Kopecny, D]]