5enz - Revision history

OCA at 08:10, 12 July 2023

2023-07-12T08:10:12Z

←Older revision		Revision as of 08:10, 12 July 2023
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	<StructureSection load='5enz' size='340' side='right'caption='[[5enz]], [[Resolution\|resolution]] 1.91Å' scene=''>		<StructureSection load='5enz' size='340' side='right'caption='[[5enz]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5enz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ENZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5ENZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5enz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ENZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ENZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.91Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mnaA, mnaA_2, AL078_07320, AM595_10995, EQ80_010635, ER12_010620, ERS365775_02407, ERS410449_02295, ERS411009_02507, ERS411017_02562, ERS445051_02067, ERS445052_01290, RL02_00620, RT87_10865, SAFDA_1985, TM61_09075 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_2-epimerase_(non-hydrolyzing) UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.14 5.1.3.14] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5enz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5enz OCA], [https://pdbe.org/5enz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5enz RCSB], [https://www.ebi.ac.uk/pdbsum/5enz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5enz ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5enz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5enz OCA], [~~http~~://pdbe.org/5enz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5enz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5enz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5enz ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9REV4_STAAU Q9REV4_STAAU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Fischmann, T O~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Dimer]]~~	+	[[Category: Fischmann TO]]
-	~~[[Category: Glycogen phosphorylase superfamily]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Sugar-nucleotide epimerase]]~~	+
-	~~[[Category: Two domain]]~~	+
-	~~[[Category: Udp~~]]	+

OCA at 07:06, 22 April 2020

2020-04-22T07:06:56Z

←Older revision		Revision as of 07:06, 22 April 2020
Line 1:		Line 1:

	==S. aureus MnaA-UDP co-structure==		==S. aureus MnaA-UDP co-structure==
-	<StructureSection load='5enz' size='340' side='right' caption='[[5enz]], [[Resolution\|resolution]] 1.91Å' scene=''>	+	<StructureSection load='5enz' size='340' side='right'caption='[[5enz]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5enz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ENZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ENZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5enz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ENZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ENZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mnaA, mnaA_2, AL078_07320, AM595_10995, EQ80_010635, ER12_010620, ERS365775_02407, ERS410449_02295, ERS411009_02507, ERS411017_02562, ERS445051_02067, ERS445052_01290, RL02_00620, RT87_10865, SAFDA_1985, TM61_09075 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_2-epimerase_(non-hydrolyzing) UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.14 5.1.3.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_2-epimerase_(non-hydrolyzing) UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.14 5.1.3.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5enz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5enz OCA], [http://pdbe.org/5enz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5enz RCSB], [http://www.ebi.ac.uk/pdbsum/5enz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5enz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5enz OCA], [http://pdbe.org/5enz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5enz RCSB], [http://www.ebi.ac.uk/pdbsum/5enz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5enz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Fischmann, T O]]		[[Category: Fischmann, T O]]
	[[Category: Dimer]]		[[Category: Dimer]]

OCA at 08:06, 1 June 2016

2016-06-01T08:06:00Z

←Older revision		Revision as of 08:06, 1 June 2016
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5enz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5enz OCA], [http://pdbe.org/5enz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5enz RCSB], [http://www.ebi.ac.uk/pdbsum/5enz PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5enz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5enz OCA], [http://pdbe.org/5enz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5enz RCSB], [http://www.ebi.ac.uk/pdbsum/5enz PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Here we describe a chemical biology strategy performed in Staphylococcus aureus and Staphylococcus epidermidis to identify MnaA, a 2-epimerase that we demonstrate interconverts UDP-GlcNAc and UDP-ManNAc to modulate substrate levels of TarO and TarA wall teichoic acid (WTA) biosynthesis enzymes. Genetic inactivation of mnaA results in complete loss of WTA and dramatic in vitro beta-lactam hypersensitivity in methicillin-resistant S. aureus (MRSA) and S. epidermidis (MRSE). Likewise, the beta-lactam antibiotic imipenem exhibits restored bactericidal activity against mnaA mutants in vitro and concomitant efficacy against 2-epimerase defective strains in a mouse thigh model of MRSA and MRSE infection. Interestingly, whereas MnaA serves as the sole 2-epimerase required for WTA biosynthesis in S. epidermidis, MnaA and Cap5P provide compensatory WTA functional roles in S. aureus. We also demonstrate that MnaA and other enzymes of WTA biosynthesis are required for biofilm formation in MRSA and MRSE. We further determine the 1.9A crystal structure of S. aureus MnaA and identify critical residues for enzymatic dimerization, stability, and substrate binding. Finally, the natural product antibiotic tunicamycin is shown to physically bind MnaA and Cap5P and inhibit 2-epimerase activity, demonstrating that it inhibits a previously unanticipated step in WTA biosynthesis. In summary, MnaA serves as a new Staphylococcal antibiotic target with cognate inhibitors predicted to possess dual therapeutic benefit: as combination agents to restore beta-lactam efficacy against MRSA and MRSE and as non-bioactive prophylactic agents to prevent Staphylococcal biofilm formation.
		+
		+	Chemical Genetic Analysis and Functional Characterization of Staphylococcal Wall Teichoic Acid 2-Epimerases Reveals Unconventional Antibiotic Drug Targets.,Mann PA, Muller A, Wolff KA, Fischmann T, Wang H, Reed P, Hou Y, Li W, Muller CE, Xiao J, Murgolo N, Sher X, Mayhood T, Sheth PR, Mirza A, Labroli M, Xiao L, McCoy M, Gill CJ, Pinho MG, Schneider T, Roemer T PLoS Pathog. 2016 May 4;12(5):e1005585. doi: 10.1371/journal.ppat.1005585., eCollection 2016 May. PMID:27144276<ref>PMID:27144276</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5enz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:03, 13 May 2016

2016-05-13T12:03:30Z

←Older revision		Revision as of 12:03, 13 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5enz ~~is ON HOLD~~	+	==S. aureus MnaA-UDP co-structure==
-		+	<StructureSection load='5enz' size='340' side='right' caption='[[5enz]], [[Resolution\|resolution]] 1.91Å' scene=''>
-	~~Authors~~: ~~Fischmann, T~~.O.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5enz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ENZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ENZ FirstGlance]. <br>
-	~~Description~~: S. ~~aureus MnaA~~-UDP co-~~structure~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_2-epimerase_(non-hydrolyzing) UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.14 5.1.3.14] </span></td></tr>
-	[[Category: Fischmann, T.O]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5enz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5enz OCA], [http://pdbe.org/5enz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5enz RCSB], [http://www.ebi.ac.uk/pdbsum/5enz PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fischmann, T O]]
		+	[[Category: Dimer]]
		+	[[Category: Glycogen phosphorylase superfamily]]
		+	[[Category: Isomerase]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Sugar-nucleotide epimerase]]
		+	[[Category: Two domain]]
		+	[[Category: Udp]]

OCA at 12:13, 3 February 2016

2016-02-03T12:13:52Z

←Older revision		Revision as of 12:13, 3 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5enz is ON HOLD ~~until Paper Publication~~	+	The entry 5enz is ON HOLD

	Authors: Fischmann, T.O.		Authors: Fischmann, T.O.

OCA: Protected "5enz" [edit=sysop:move=sysop]

2015-12-01T03:06:53Z

Protected "5enz" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:06, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5enz is ON HOLD until Paper Publication Authors: Fischmann, T.O. Description: S. aureus MnaA-UDP co-structure Category: Unreleased Structures [...

2015-12-01T03:06:51Z

New page: '''Unreleased structure''' The entry 5enz is ON HOLD until Paper Publication Authors: Fischmann, T.O. Description: S. aureus MnaA-UDP co-structure Category: Unreleased Structures [...

New page

'''Unreleased structure'''

The entry 5enz is ON HOLD until Paper Publication

Authors: Fischmann, T.O.

Description: S. aureus MnaA-UDP co-structure
[[Category: Unreleased Structures]]
[[Category: Fischmann, T.O]]