5eou - Revision history

OCA at 08:12, 12 July 2023

2023-07-12T08:12:06Z

←Older revision		Revision as of 08:12, 12 July 2023
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	<StructureSection load='5eou' size='340' side='right'caption='[[5eou]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5eou' size='340' side='right'caption='[[5eou]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5eou]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EOU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5eou]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EOU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eox\|5eox]], [[5eoy\|5eoy]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pilN, PA5043 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eou OCA], [https://pdbe.org/5eou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eou RCSB], [https://www.ebi.ac.uk/pdbsum/5eou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eou ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5eou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eou OCA], [~~http~~://pdbe.org/5eou PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5eou RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5eou PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5eou ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PILM_PSEAE PILM_PSEAE] Inner membrane component of the type IV (T4S) secretion system that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility. PilN/PilO heterodimers form the foundation of the inner-membrane PilM/PilN/PilO/PilP complex which plays an essential role in the assembly of a functional T4 pilus (PubMed:19857645, PubMed:19857646). In turn, associates with PilN and facilitates PilM functionally relevant structural changes that differentially impacts PilM binding to PilB, PilT, and PilC (PubMed:27022027).<ref>PMID:19857645</ref> <ref>PMID:19857646</ref> <ref>PMID:27022027</ref> [https://www.uniprot.org/uniprot/PILN_PSEAE PILN_PSEAE] Inner membrane component of the type IV (T4S) secretion system that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility. PilN/PilO heterodimers form the foundation of the inner-membrane PilM/PilN/PilO/PilP complex which plays an essential role in the assembly of a functional T4 pilus (PubMed:19857645, PubMed:19857646). In turn, associates with PilM and facilitates PilM functionally relevant structural changes (PubMed:27022027).<ref>PMID:19857645</ref> <ref>PMID:19857646</ref> <ref>PMID:27022027</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Burrows, L]]	+	[[Category: Burrows L]]
-	[[Category: Calmettes, C]]	+	[[Category: Calmettes C]]
-	[[Category: Howell~~, L P~~]]	+	[[Category: Howell LP]]
-	[[Category: McCallum, M]]	+	[[Category: McCallum M]]
-	[[Category: Moraes, T]]	+	[[Category: Moraes T]]
-	[[Category: Robinson, H]]	+	[[Category: Robinson H]]
-	[[Category: Shah, M]]	+	[[Category: Shah M]]
-	[[Category: Tammam, S]]	+	[[Category: Tammam S]]
-	~~[[Category: Peptide binding protein]]~~	+
-	~~[[Category: Pilm]]~~	+
-	~~[[Category: Piln]]~~	+
-	~~[[Category: T4p]]~~	+
-	~~[[Category: Type iv pilus~~]]	+

OCA at 07:08, 22 April 2020

2020-04-22T07:08:06Z

←Older revision		Revision as of 07:08, 22 April 2020
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	==Pseudomonas aeruginosa PilM:PilN1-12 bound to ATP==		==Pseudomonas aeruginosa PilM:PilN1-12 bound to ATP==
-	<StructureSection load='5eou' size='340' side='right' caption='[[5eou]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='5eou' size='340' side='right'caption='[[5eou]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5eou]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOU OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EOU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5eou]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EOU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eox\|5eox]], [[5eoy\|5eoy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eox\|5eox]], [[5eoy\|5eoy]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5eou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eou OCA], [http://pdbe.org/5eou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eou RCSB], [http://www.ebi.ac.uk/pdbsum/5eou PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pilN, PA5043 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5eou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eou OCA], [http://pdbe.org/5eou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eou RCSB], [http://www.ebi.ac.uk/pdbsum/5eou PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eou ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseae]]
	[[Category: Burrows, L]]		[[Category: Burrows, L]]
	[[Category: Calmettes, C]]		[[Category: Calmettes, C]]

OCA at 12:03, 13 May 2016

2016-05-13T12:03:41Z

←Older revision		Revision as of 12:03, 13 May 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5eou is ~~ON HOLD until Paper~~ Publication	+	==Pseudomonas aeruginosa PilM:PilN1-12 bound to ATP==
		+	<StructureSection load='5eou' size='340' side='right' caption='[[5eou]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5eou]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EOU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eox\|5eox]], [[5eoy\|5eoy]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eou OCA], [http://pdbe.org/5eou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eou RCSB], [http://www.ebi.ac.uk/pdbsum/5eou PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pseudomonas aeruginosais an opportunistic bacterial pathogen that expresses type IVa pili (T4aP). The pilus assembly system, which promotes surface-associated twitching motility and virulence, is comprised of inner and outer membrane subcomplexes, connected by an alignment subcomplex composed of PilMNOP. PilM binds to the N-terminus of PilN, and we hypothesize that this interaction causes functionally significant structural changes in PilM. To characterize this interaction, we determined the crystal structures of PilM and a PilM chimera where PilM was fused to the first twelve residues of PilN (PilM:PilN1-12). Structural analysis, multi-angle light scattering coupled with size exclusion chromatography, and bacterial two-hybrid (BACTH) data revealed that PilM forms dimers mediated by the binding of a novel conserved motif in the N-terminus of PilM, and binding PilN abrogates this binding interface resulting in PilM monomerization. Structural comparison of PilM to PilM:PilN1-12 revealed that upon PilN binding there is a large domain closure in PilM that alters its ATP binding site. Using biolayer interferometry we found that the association rate of PilN to PilM is higher in the presence of ATP compared to ADP. BACTH data suggested the connectivity of the cytoplasmic and inner membrane components of the T4aP machinery inP. aeruginosa, with PilM binding to PilB, PilT, and PilC, in addition to PilN. Pull-down experiments demonstrated direct interactions of PilM with PilB and PilT. We propose a working model in which dynamic binding of PilN facilitates functionally relevant structural changes in PilM.

-	~~Authors: McCallum~~, M., Tammam, S., Robinson, H., Shah, M., Calmettes, C., Moraes~~, T.~~, Burrows~~, L.~~, Howell~~, L~~.P.	+	PilN binding modulates the structure and binding partners of the Pseudomonas aeruginosa Type IVa Pilus protein PilM.,McCallum M, Tammam S, Little DJ, Robinson H, Koo J, Shah M, Calmettes C, Moraes TF, Burrows LL, Howell PL J Biol Chem. 2016 Mar 28. pii: jbc.M116.718353. PMID:27022027<ref>PMID:27022027</ref>

-	~~Description:~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Robinson, H]]~~	+	<div class="pdbe-citations 5eou" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Shah~~, M]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Burrows, L]]
	[[Category: Calmettes, C]]		[[Category: Calmettes, C]]
		+	[[Category: Howell, L P]]
		+	[[Category: McCallum, M]]
	[[Category: Moraes, T]]		[[Category: Moraes, T]]
-	[[Category: ~~Howell~~, ~~L.P~~]]	+	[[Category: Robinson, H]]
-	[[Category: ~~Burrows~~, L]]	+	[[Category: Shah, M]]
	[[Category: Tammam, S]]		[[Category: Tammam, S]]
-	[[Category: ~~Mccallum, M~~]]	+	[[Category: Peptide binding protein]]
		+	[[Category: Pilm]]
		+	[[Category: Piln]]
		+	[[Category: T4p]]
		+	[[Category: Type iv pilus]]

OCA at 12:06, 9 December 2015

2015-12-09T12:06:14Z

←Older revision		Revision as of 12:06, 9 December 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5eou is ON HOLD	+	The entry 5eou is ON HOLD until Paper Publication

	Authors: McCallum, M., Tammam, S., Robinson, H., Shah, M., Calmettes, C., Moraes, T., Burrows, L., Howell, L.P.		Authors: McCallum, M., Tammam, S., Robinson, H., Shah, M., Calmettes, C., Moraes, T., Burrows, L., Howell, L.P.

OCA: Protected "5eou" [edit=sysop:move=sysop]

2015-12-01T03:08:55Z

Protected "5eou" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:08, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5eou is ON HOLD Authors: McCallum, M., Tammam, S., Robinson, H., Shah, M., Calmettes, C., Moraes, T., Burrows, L., Howell, L.P. Description: [[Cat...

2015-12-01T03:08:54Z

New page: '''Unreleased structure''' The entry 5eou is ON HOLD Authors: McCallum, M., Tammam, S., Robinson, H., Shah, M., Calmettes, C., Moraes, T., Burrows, L., Howell, L.P. Description: [[Cat...

New page

'''Unreleased structure'''

The entry 5eou is ON HOLD

Authors: McCallum, M., Tammam, S., Robinson, H., Shah, M., Calmettes, C., Moraes, T., Burrows, L., Howell, L.P.

Description:
[[Category: Unreleased Structures]]
[[Category: Robinson, H]]
[[Category: Shah, M]]
[[Category: Calmettes, C]]
[[Category: Moraes, T]]
[[Category: Howell, L.P]]
[[Category: Burrows, L]]
[[Category: Tammam, S]]
[[Category: Mccallum, M]]