5ewi - Revision history

OCA at 08:29, 12 July 2023

2023-07-12T08:29:06Z

←Older revision		Revision as of 08:29, 12 July 2023
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	<StructureSection load='5ewi' size='340' side='right'caption='[[5ewi]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='5ewi' size='340' side='right'caption='[[5ewi]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ewi]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EWI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EWI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ewi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EWI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ewi OCA], [~~http~~://pdbe.org/5ewi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ewi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ewi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ewi ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5996Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ewi OCA], [https://pdbe.org/5ewi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ewi RCSB], [https://www.ebi.ac.uk/pdbsum/5ewi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ewi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6N089_HUMAN Q6N089_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gorman, J]]	+	[[Category: Gorman J]]
-	[[Category: Kwong~~, P D]]~~	+	[[Category: Kwong PD]]
-	~~[[Category: Antibody]]~~	+
-	~~[[Category: Env]]~~	+
-	~~[[Category: Envelope]]~~	+
-	~~[[Category: Fab]]~~	+
-	~~[[Category: Hiv-1]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Neutralizing]]~~	+
-	~~[[Category: V1v2~~]]	+

OCA at 07:47, 29 April 2020

2020-04-29T07:47:40Z

←Older revision		Revision as of 07:47, 29 April 2020
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	==Crystal Structure of the Human Fab VRC38.01, an HIV-1 V1V2-Directed Neutralizing Antibody Isolated from Donor N90==		==Crystal Structure of the Human Fab VRC38.01, an HIV-1 V1V2-Directed Neutralizing Antibody Isolated from Donor N90==
-	<StructureSection load='5ewi' size='340' side='right' caption='[[5ewi]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='5ewi' size='340' side='right'caption='[[5ewi]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ewi]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EWI OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EWI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ewi]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EWI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EWI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ewi OCA], [http://pdbe.org/5ewi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ewi RCSB], [http://www.ebi.ac.uk/pdbsum/5ewi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ewi ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ewi OCA], [http://pdbe.org/5ewi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ewi RCSB], [http://www.ebi.ac.uk/pdbsum/5ewi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ewi ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Gorman, J]]		[[Category: Gorman, J]]
	[[Category: Kwong, P D]]		[[Category: Kwong, P D]]

OCA at 09:33, 3 August 2017

2017-08-03T09:33:53Z

←Older revision		Revision as of 09:33, 3 August 2017
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	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ewi OCA], [http://pdbe.org/5ewi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ewi RCSB], [http://www.ebi.ac.uk/pdbsum/5ewi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ewi ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ewi OCA], [http://pdbe.org/5ewi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ewi RCSB], [http://www.ebi.ac.uk/pdbsum/5ewi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ewi ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Most HIV-1-specific neutralizing antibodies isolated to date exhibit unusual characteristics that complicate their elicitation. Neutralizing antibodies that target the V1V2 apex of the HIV-1 envelope (Env) trimer feature unusually long protruding loops, which enable them to penetrate the HIV-1 glycan shield. As antibodies with loops of requisite length are created through uncommon recombination events, an alternative mode of apex binding has been sought. Here, we isolated a lineage of Env apex-directed neutralizing antibodies, N90-VRC38.01-11, by using virus-like particles and conformationally stabilized Env trimers as B cell probes. A crystal structure of N90-VRC38.01 with a scaffolded V1V2 revealed a binding mode involving side-chain-to-side-chain interactions that reduced the distance the antibody loop must traverse the glycan shield, thereby facilitating V1V2 binding via a non-protruding loop. The N90-VRC38 lineage thus identifies a solution for V1V2-apex binding that provides a more conventional B cell pathway for vaccine design.
		+
		+	Virus-like Particles Identify an HIV V1V2 Apex-Binding Neutralizing Antibody that Lacks a Protruding Loop.,Cale EM, Gorman J, Radakovich NA, Crooks ET, Osawa K, Tong T, Li J, Nagarajan R, Ozorowski G, Ambrozak DR, Asokan M, Bailer RT, Bennici AK, Chen X, Doria-Rose NA, Druz A, Feng Y, Joyce MG, Louder MK, O'Dell S, Oliver C, Pancera M, Connors M, Hope TJ, Kepler TB, Wyatt RT, Ward AB, Georgiev IS, Kwong PD, Mascola JR, Binley JM Immunity. 2017 May 16;46(5):777-791.e10. doi: 10.1016/j.immuni.2017.04.011. PMID:28514685<ref>PMID:28514685</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ewi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:50, 10 December 2016

2016-12-10T10:50:05Z

←Older revision		Revision as of 10:50, 10 December 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5ewi is ~~ON HOLD until Paper Publication~~	+	==Crystal Structure of the Human Fab VRC38.01, an HIV-1 V1V2-Directed Neutralizing Antibody Isolated from Donor N90==
-		+	<StructureSection load='5ewi' size='340' side='right' caption='[[5ewi]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ewi]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EWI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EWI FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ewi OCA], [http://pdbe.org/5ewi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ewi RCSB], [http://www.ebi.ac.uk/pdbsum/5ewi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ewi ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gorman, J]]
		+	[[Category: Kwong, P D]]
		+	[[Category: Antibody]]
		+	[[Category: Env]]
		+	[[Category: Envelope]]
		+	[[Category: Fab]]
		+	[[Category: Hiv-1]]
		+	[[Category: Immune system]]
		+	[[Category: Neutralizing]]
		+	[[Category: V1v2]]

OCA at 12:38, 15 May 2016

2016-05-15T12:38:02Z

←Older revision		Revision as of 12:38, 15 May 2016
Line 3:		Line 3:
	The entry 5ewi is ON HOLD until Paper Publication		The entry 5ewi is ON HOLD until Paper Publication

-	Authors: ~~Gorman, J., Kwong, P.D.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Kwong, P.D]]
-	[[Category: Gorman, J]]

OCA at 12:19, 16 December 2015

2015-12-16T12:19:01Z

←Older revision		Revision as of 12:19, 16 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ewi is ON HOLD	+	The entry 5ewi is ON HOLD until Paper Publication

	Authors: Gorman, J., Kwong, P.D.		Authors: Gorman, J., Kwong, P.D.

OCA: Protected "5ewi" [edit=sysop:move=sysop]

2015-12-02T15:04:48Z

Protected "5ewi" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:04, 2 December 2015

OCA: New page: '''Unreleased structure''' The entry 5ewi is ON HOLD Authors: Gorman, J., Kwong, P.D. Description: Category: Unreleased Structures Category: Kwong, P.D Category: Gorman, J

2015-12-02T15:04:46Z

New page: '''Unreleased structure''' The entry 5ewi is ON HOLD Authors: Gorman, J., Kwong, P.D. Description: Category: Unreleased Structures Category: Kwong, P.D Category: Gorman, J

New page

'''Unreleased structure'''

The entry 5ewi is ON HOLD

Authors: Gorman, J., Kwong, P.D.

Description:
[[Category: Unreleased Structures]]
[[Category: Kwong, P.D]]
[[Category: Gorman, J]]