5eyx - Revision history

OCA at 08:34, 12 July 2023

2023-07-12T08:34:44Z

←Older revision		Revision as of 08:34, 12 July 2023
Line 3:		Line 3:
	<StructureSection load='5eyx' size='340' side='right'caption='[[5eyx]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='5eyx' size='340' side='right'caption='[[5eyx]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5eyx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Centrolobium_tomentosum Centrolobium tomentosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EYX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EYX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5eyx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Centrolobium_tomentosum Centrolobium tomentosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EYX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=~~MDM~~:~~METHYL-O3-(~~ALPHA-D-MANNOSE)-~~ALPHA-D~~-MANNOSE'>~~MDM~~</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eyy\|5eyy]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5eyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eyx OCA], [~~http~~://pdbe.org/5eyx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5eyx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5eyx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5eyx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eyx OCA], [https://pdbe.org/5eyx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eyx RCSB], [https://www.ebi.ac.uk/pdbsum/5eyx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eyx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LECC1_CENTO LECC1_CENTO] Mannose/glucose-specific lectin that also binds derivatives N-acetyl-D-glucosamine and alpha-methyl-D-mannopyranoside with even higher affinity (Ref.2, PubMed:26946944). Has hemagglutinating activity towards rabbit erythrocytes (Ref.2). Is toxic towards brine shrimp A.nauplii (Ref.2). In rats, induces dose-dependent paw edema (PubMed:26946944).<ref>PMID:26946944</ref> [REFERENCE:2]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 25:
	[[Category: Centrolobium tomentosum]]		[[Category: Centrolobium tomentosum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Almeida~~, A C~~]]	+	[[Category: Almeida AC]]
-	[[Category: Almeida~~, R P.H~~]]	+	[[Category: Almeida RPH]]
-	[[Category: Cavada~~, B S~~]]	+	[[Category: Cavada BS]]
-	[[Category: Delatorre, P]]	+	[[Category: Delatorre P]]
-	[[Category: Lossio~~, C F~~]]	+	[[Category: Lossio CF]]
-	[[Category: Osterne~~, V J.S~~]]	+	[[Category: Osterne VJS]]
-	[[Category: Pinto-Junior~~, V R~~]]	+	[[Category: Pinto-Junior VR]]
-	[[Category: Rocha~~, B A.M~~]]	+	[[Category: Rocha BAM]]
-	[[Category: Santiago~~, K S~~]]	+	[[Category: Santiago KS]]
-	[[Category: Santiago~~, M Q~~]]	+	[[Category: Santiago MQ]]
-	[[Category: Silva-Filho~~, J C~~]]	+	[[Category: Silva-Filho JC]]
-	[[Category: Teixeira~~, C S]]~~	+	[[Category: Teixeira CS]]
-	~~[[Category: Ctl]]~~	+
-	~~[[Category: Dalbergieae]]~~	+
-	~~[[Category: Lectin]]~~	+
-	~~[[Category: Methyl dimannoside]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 07:51, 29 April 2020

2020-04-29T07:51:17Z

←Older revision		Revision as of 07:51, 29 April 2020
Line 1:		Line 1:

	==Monoclinic Form of Centrolobium tomentosum seed lectin (CTL) complexed with Man1-3Man-OMe.==		==Monoclinic Form of Centrolobium tomentosum seed lectin (CTL) complexed with Man1-3Man-OMe.==
-	<StructureSection load='5eyx' size='340' side='right' caption='[[5eyx]], [[Resolution\|resolution]] 2.25Å' scene=''>	+	<StructureSection load='5eyx' size='340' side='right'caption='[[5eyx]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5eyx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Centrolobium_tomentosum Centrolobium tomentosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EYX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EYX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5eyx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Centrolobium_tomentosum Centrolobium tomentosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EYX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EYX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MDM:METHYL-O3-(ALPHA-D-MANNOSE)-ALPHA-D-MANNOSE'>MDM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MDM:METHYL-O3-(ALPHA-D-MANNOSE)-ALPHA-D-MANNOSE'>MDM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eyy\|5eyy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eyy\|5eyy]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5eyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eyx OCA], [http://pdbe.org/5eyx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eyx RCSB], [http://www.ebi.ac.uk/pdbsum/5eyx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5eyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eyx OCA], [http://pdbe.org/5eyx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eyx RCSB], [http://www.ebi.ac.uk/pdbsum/5eyx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eyx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Centrolobium tomentosum]]		[[Category: Centrolobium tomentosum]]
		+	[[Category: Large Structures]]
	[[Category: Almeida, A C]]		[[Category: Almeida, A C]]
	[[Category: Almeida, R P.H]]		[[Category: Almeida, R P.H]]

OCA at 16:55, 10 May 2016

2016-05-10T16:55:01Z

←Older revision		Revision as of 16:55, 10 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5eyx is ~~ON HOLD until Paper~~ Publication	+	==Monoclinic Form of Centrolobium tomentosum seed lectin (CTL) complexed with Man1-3Man-OMe.==
		+	<StructureSection load='5eyx' size='340' side='right' caption='[[5eyx]], [[Resolution\|resolution]] 2.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5eyx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Centrolobium_tomentosum Centrolobium tomentosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EYX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EYX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MDM:METHYL-O3-(ALPHA-D-MANNOSE)-ALPHA-D-MANNOSE'>MDM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eyy\|5eyy]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eyx OCA], [http://pdbe.org/5eyx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eyx RCSB], [http://www.ebi.ac.uk/pdbsum/5eyx PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A glycosylated lectin (CTL) with specificity for mannose and glucose has been detected and purified from seeds of Centrolobium tomentosum, a legume plant from Dalbergieae tribe. It was isolated by mannose-sepharose affinity chromatography. The primary structure was determined by tandem mass spectrometry and consists of 245 amino acids, similar to other Dalbergieae lectins. CTL structures were solved from two crystal forms, a monoclinic and a tetragonal, diffracted at 2.25 and 1.9 A, respectively. The carbohydrate recognition domain (CRD), metal-binding site and glycosylation site were characterized, and the structural basis for mannose/glucose-binding was elucidated. The lectin adopts the canonical dimeric organization of legume lectins. CTL showed acute inflammatory effect in paw edema model. The protein was subjected to ligand screening (dimannosides and trimannoside) by molecular docking, and interactions were compared with similar lectins possessing the same ligand specificity. This is the first crystal structure of mannose/glucose native seed lectin with proinflammatory activity isolated from the Centrolobium genus.

-	~~Authors:~~ Almeida~~, A.C.~~, Osterne~~, V.J.S.~~, Santiago~~, M.Q.~~, Pinto-Junior~~, V.R.~~, Silva-Filho~~, J.C.~~, Lossio, ~~C.F.~~, Almeida~~, R.P.H.~~, Teixeira, ~~C.S.~~, Delatorre, P., Rocha, B.A.M.~~, Santiago, K~~.S., ~~Cavada, B.S~~.	+	Structural analysis of Centrolobium tomentosum seed lectin with inflammatory activity.,Almeida AC, Osterne VJ, Santiago MQ, Pinto-Junior VR, Silva-Filho JC, Lossio CF, Nascimento FL, Almeida RP, Teixeira CS, Leal RB, Delatorre P, Rocha BA, Assreuy AM, Nascimento KS, Cavada BS Arch Biochem Biophys. 2016 Apr 15;596:73-83. doi: 10.1016/j.abb.2016.03.001. Epub, 2016 Mar 3. PMID:26946944<ref>PMID:26946944</ref>

-	~~Description: Monoclinic Form~~ of ~~Centrolobium tomentosum seed lectin (CTL) complexed with Man1-3Man-OMe~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Lossio, C.F]]~~	+	<div class="pdbe-citations 5eyx" style="background-color:#fffaf0;"></div>
-	~~[[Category: Rocha, B.A.M]]~~	+	== References ==
-	~~[[Category: Teixeira, C.S]]~~	+	<references/>
-	~~[[Category: Silva-Filho, J.C]]~~	+	__TOC__
-	[[Category: ~~Pinto-Junior, V.R~~]]	+	</StructureSection>
-	[[Category: Almeida, A.C]]	+	[[Category: Centrolobium tomentosum]]
-	[[Category: ~~Santiago~~, M.~~Q]]~~	+	[[Category: Almeida, A C]]
-	~~[[Category: Osterne, V.J.S~~]]	+	[[Category: Almeida, R P.H]]
-	[[Category: Cavada, B.S]]	+	[[Category: Cavada, B S]]
-	~~[[Category: Almeida, R.P.H~~]]	+
	[[Category: Delatorre, P]]		[[Category: Delatorre, P]]
-	[[Category: Santiago, K.S]]	+	[[Category: Lossio, C F]]
		+	[[Category: Osterne, V J.S]]
		+	[[Category: Pinto-Junior, V R]]
		+	[[Category: Rocha, B A.M]]
		+	[[Category: Santiago, K S]]
		+	[[Category: Santiago, M Q]]
		+	[[Category: Silva-Filho, J C]]
		+	[[Category: Teixeira, C S]]
		+	[[Category: Ctl]]
		+	[[Category: Dalbergieae]]
		+	[[Category: Lectin]]
		+	[[Category: Methyl dimannoside]]
		+	[[Category: Sugar binding protein]]

OCA at 12:22, 16 December 2015

2015-12-16T12:22:48Z

←Older revision		Revision as of 12:22, 16 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5eyx is ON HOLD	+	The entry 5eyx is ON HOLD until Paper Publication

	Authors: Almeida, A.C., Osterne, V.J.S., Santiago, M.Q., Pinto-Junior, V.R., Silva-Filho, J.C., Lossio, C.F., Almeida, R.P.H., Teixeira, C.S., Delatorre, P., Rocha, B.A.M., Santiago, K.S., Cavada, B.S.		Authors: Almeida, A.C., Osterne, V.J.S., Santiago, M.Q., Pinto-Junior, V.R., Silva-Filho, J.C., Lossio, C.F., Almeida, R.P.H., Teixeira, C.S., Delatorre, P., Rocha, B.A.M., Santiago, K.S., Cavada, B.S.

OCA at 12:23, 9 December 2015

2015-12-09T12:23:00Z

←Older revision		Revision as of 12:23, 9 December 2015
Line 3:		Line 3:
	The entry 5eyx is ON HOLD		The entry 5eyx is ON HOLD

-	Authors: Almeida, A. C., Osterne, V.J.S., Santiago, M.Q., Pinto-Junior, V.R., Silva-Filho, J.C., Lossio, C.F., Almeida, R.P.H., Teixeira, C.S., Delatorre, P., Rocha, B.A.M., Santiago, K.S., Cavada, B.S.	+	Authors: Almeida, A.C., Osterne, V.J.S., Santiago, M.Q., Pinto-Junior, V.R., Silva-Filho, J.C., Lossio, C.F., Almeida, R.P.H., Teixeira, C.S., Delatorre, P., Rocha, B.A.M., Santiago, K.S., Cavada, B.S.

	Description: Monoclinic Form of Centrolobium tomentosum seed lectin (CTL) complexed with Man1-3Man-OMe.		Description: Monoclinic Form of Centrolobium tomentosum seed lectin (CTL) complexed with Man1-3Man-OMe.
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Lossio, C.F]]		[[Category: Lossio, C.F]]
-	[[Category: Almeida, A. C]]
	[[Category: Rocha, B.A.M]]		[[Category: Rocha, B.A.M]]
	[[Category: Teixeira, C.S]]		[[Category: Teixeira, C.S]]
	[[Category: Silva-Filho, J.C]]		[[Category: Silva-Filho, J.C]]
	[[Category: Pinto-Junior, V.R]]		[[Category: Pinto-Junior, V.R]]
		+	[[Category: Almeida, A.C]]
	[[Category: Santiago, M.Q]]		[[Category: Santiago, M.Q]]
	[[Category: Osterne, V.J.S]]		[[Category: Osterne, V.J.S]]

OCA: Protected "5eyx" [edit=sysop:move=sysop]

2015-12-02T15:11:01Z

Protected "5eyx" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:11, 2 December 2015

OCA: New page: '''Unreleased structure''' The entry 5eyx is ON HOLD Authors: Almeida, A. C., Osterne, V.J.S., Santiago, M.Q., Pinto-Junior, V.R., Silva-Filho, J.C., Lossio, C.F., Almeida, R.P.H., Teix...

2015-12-02T15:10:59Z

New page: '''Unreleased structure''' The entry 5eyx is ON HOLD Authors: Almeida, A. C., Osterne, V.J.S., Santiago, M.Q., Pinto-Junior, V.R., Silva-Filho, J.C., Lossio, C.F., Almeida, R.P.H., Teix...

New page

'''Unreleased structure'''

The entry 5eyx is ON HOLD

Authors: Almeida, A. C., Osterne, V.J.S., Santiago, M.Q., Pinto-Junior, V.R., Silva-Filho, J.C., Lossio, C.F., Almeida, R.P.H., Teixeira, C.S., Delatorre, P., Rocha, B.A.M., Santiago, K.S., Cavada, B.S.

Description: Monoclinic Form of Centrolobium tomentosum seed lectin (CTL) complexed with Man1-3Man-OMe.
[[Category: Unreleased Structures]]
[[Category: Lossio, C.F]]
[[Category: Almeida, A. C]]
[[Category: Rocha, B.A.M]]
[[Category: Teixeira, C.S]]
[[Category: Silva-Filho, J.C]]
[[Category: Pinto-Junior, V.R]]
[[Category: Santiago, M.Q]]
[[Category: Osterne, V.J.S]]
[[Category: Cavada, B.S]]
[[Category: Almeida, R.P.H]]
[[Category: Delatorre, P]]
[[Category: Santiago, K.S]]