5ezy - Revision history

OCA at 08:37, 12 July 2023

2023-07-12T08:37:04Z

←Older revision		Revision as of 08:37, 12 July 2023
Line 3:		Line 3:
	<StructureSection load='5ezy' size='340' side='right'caption='[[5ezy]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='5ezy' size='340' side='right'caption='[[5ezy]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ezy]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/ ], [~~http~~://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~] and [~~http~~://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EZY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ezy]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus], [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus], [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EZY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TAJ:TACCALONOLIDE+AJ'>TAJ</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.05Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ezy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ezy OCA], [~~http~~://pdbe.org/5ezy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ezy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ezy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ezy ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TAJ:TACCALONOLIDE+AJ'>TAJ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ezy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ezy OCA], [https://pdbe.org/5ezy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ezy RCSB], [https://www.ebi.ac.uk/pdbsum/5ezy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ezy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT]] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref> [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain ~~(By similarity)~~.	+	[https://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Rattus norvegicus]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: Chen, Q]]	+	[[Category: Chen Q]]
-	[[Category: Wang, Y]]	+	[[Category: Wang Y]]
-	[[Category: Yang, J]]	+	[[Category: Yang J]]
-	[[Category: Yu, Y]]	+	[[Category: Yu Y]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Structural protein]]~~	+
-	~~[[Category: Tubulin~~]]	+

OCA at 07:53, 29 April 2020

2020-04-29T07:53:11Z

←Older revision		Revision as of 07:53, 29 April 2020
Line 1:		Line 1:

	==Crystal structure of T2R-TTL-taccalonolide AJ complex==		==Crystal structure of T2R-TTL-taccalonolide AJ complex==
-	<StructureSection load='5ezy' size='340' side='right' caption='[[5ezy]], [[Resolution\|resolution]] 2.05Å' scene=''>	+	<StructureSection load='5ezy' size='340' side='right'caption='[[5ezy]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ezy]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ], [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZY OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EZY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ezy]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ], [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EZY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TAJ:TACCALONOLIDE+AJ'>TAJ</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TAJ:TACCALONOLIDE+AJ'>TAJ</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ezy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ezy OCA], [http://pdbe.org/5ezy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ezy RCSB], [http://www.ebi.ac.uk/pdbsum/5ezy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ezy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ezy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ezy OCA], [http://pdbe.org/5ezy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ezy RCSB], [http://www.ebi.ac.uk/pdbsum/5ezy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ezy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5ezy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5ezy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Stathmin-4 3D structures\|Stathmin-4 3D structures]]
		+	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Chen, Q]]		[[Category: Chen, Q]]

OCA at 10:37, 3 August 2017

2017-08-03T10:37:18Z

←Older revision		Revision as of 10:37, 3 August 2017
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT]] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref> [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).		[[http://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT]] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref> [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	As a major component of the cytoskeleton, microtubules consist of alphabeta-tubulin heterodimers and have been recognized as attractive targets for cancer chemotherapy. Microtubule-stabilizing agents (MSAs) promote polymerization of tubulin and stabilize the polymer, preventing depolymerization. The molecular mechanisms by which MSAs stabilize microtubules remain elusive. Here we report a 2.05 A crystal structure of tubulin complexed with taccalonolide AJ, a newly identified taxane-site MSA. Taccalonolide AJ covalently binds to beta-tubulin D226. On AJ binding, the M-loop undergoes a conformational shift to facilitate tubulin polymerization. In this tubulin-AJ complex, the E-site of tubulin is occupied by GTP rather than GDP. Biochemical analyses confirm that AJ inhibits the hydrolysis of the E-site GTP. Thus, we propose that the beta-tubulin E-site is locked into a GTP-preferred status by AJ binding. Our results provide experimental evidence for the connection between MSA binding and tubulin nucleotide state, and will help design new MSAs to overcome taxane resistance.
		+
		+	Mechanism of microtubule stabilization by taccalonolide AJ.,Wang Y, Yu Y, Li GB, Li SA, Wu C, Gigant B, Qin W, Chen H, Wu Y, Chen Q, Yang J Nat Commun. 2017 Jun 6;8:15787. doi: 10.1038/ncomms15787. PMID:28585532<ref>PMID:28585532</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ezy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:24, 18 January 2017

2017-01-18T16:24:51Z

←Older revision		Revision as of 16:24, 18 January 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ezy ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of T2R-TTL-taccalonolide AJ complex==
-		+	<StructureSection load='5ezy' size='340' side='right' caption='[[5ezy]], [[Resolution\|resolution]] 2.05Å' scene=''>
-	~~Authors~~: ~~Wang~~, Y., Yu, Y., ~~Chen~~, Q., ~~Yang~~, J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ezy]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ], [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EZY FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of ~~T2R~~-~~TTL~~-~~taccalonolide AJ complex~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TAJ:TACCALONOLIDE+AJ'>TAJ</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ezy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ezy OCA], [http://pdbe.org/5ezy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ezy RCSB], [http://www.ebi.ac.uk/pdbsum/5ezy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ezy ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT]] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref> [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bos taurus]]
		+	[[Category: Sus scrofa]]
		+	[[Category: Chen, Q]]
	[[Category: Wang, Y]]		[[Category: Wang, Y]]
	[[Category: Yang, J]]		[[Category: Yang, J]]
	[[Category: Yu, Y]]		[[Category: Yu, Y]]
-	[[Category: ~~Chen, Q~~]]	+	[[Category: Complex]]
		+	[[Category: Inhibitor]]
		+	[[Category: Structural protein]]
		+	[[Category: Tubulin]]

OCA at 12:24, 16 December 2015

2015-12-16T12:24:32Z

←Older revision		Revision as of 12:24, 16 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ezy is ON HOLD	+	The entry 5ezy is ON HOLD until Paper Publication

	Authors: Wang, Y., Yu, Y., Chen, Q., Yang, J.		Authors: Wang, Y., Yu, Y., Chen, Q., Yang, J.

OCA: Protected "5ezy" [edit=sysop:move=sysop]

2015-12-02T15:13:58Z

Protected "5ezy" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:13, 2 December 2015

OCA: New page: '''Unreleased structure''' The entry 5ezy is ON HOLD Authors: Wang, Y., Yu, Y., Chen, Q., Yang, J. Description: Crystal structure of T2R-TTL-taccalonolide AJ complex [[Category: Unrele...

2015-12-02T15:13:56Z

New page: '''Unreleased structure''' The entry 5ezy is ON HOLD Authors: Wang, Y., Yu, Y., Chen, Q., Yang, J. Description: Crystal structure of T2R-TTL-taccalonolide AJ complex [[Category: Unrele...

New page

'''Unreleased structure'''

The entry 5ezy is ON HOLD

Authors: Wang, Y., Yu, Y., Chen, Q., Yang, J.

Description: Crystal structure of T2R-TTL-taccalonolide AJ complex
[[Category: Unreleased Structures]]
[[Category: Wang, Y]]
[[Category: Yang, J]]
[[Category: Yu, Y]]
[[Category: Chen, Q]]