5f5q - Revision history

OCA at 08:48, 12 July 2023

2023-07-12T08:48:32Z

←Older revision		Revision as of 08:48, 12 July 2023
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	<StructureSection load='5f5q' size='340' side='right'caption='[[5f5q]], [[Resolution\|resolution]] 2.52Å' scene=''>		<StructureSection load='5f5q' size='340' side='right'caption='[[5f5q]], [[Resolution\|resolution]] 2.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5f5q]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Canavalia_cathartica Canavalia cathartica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5F5Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5f5q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_cathartica Canavalia cathartica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F5Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.52Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5f5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5q OCA], [~~http~~://pdbe.org/5f5q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5f5q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5f5q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5q ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5q OCA], [https://pdbe.org/5f5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f5q RCSB], [https://www.ebi.ac.uk/pdbsum/5f5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~CONA_CANCT CONA_CANCT~~]~~] Glucose/~~D-mannose ~~specific~~ lectin.	+	[https://www.uniprot.org/uniprot/CONV_CANCT CONV_CANCT] D-mannose/D-glucose-binding lectin which binds alpha-methyl-D-mannoside, D-mannose and D-glucose in that order (PubMed:24519628, PubMed:27737777). Also binds to serum fetuin and ovalbumin (PubMed:24519628). Has hemagglutinating activity towards rabbit erythrocytes (PubMed:24519628). Is not toxic towards larvae of the brine shrimp Artemia (PubMed:24519628). Induces relaxation in rat endothelized aorta (PubMed:24519628). Shows a transient edematogenic effect in rat (PubMed:27737777).<ref>PMID:24519628</ref> <ref>PMID:27737777</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Canavalia cathartica]]		[[Category: Canavalia cathartica]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cavada~~, B S~~]]	+	[[Category: Cavada BS]]
-	[[Category: Delatorre, P]]	+	[[Category: Delatorre P]]
-	[[Category: Lossio~~, C F~~]]	+	[[Category: Lossio CF]]
-	[[Category: Nascimento~~, K S~~]]	+	[[Category: Nascimento KS]]
-	[[Category: Osterne~~, V J.S~~]]	+	[[Category: Osterne VJS]]
-	[[Category: Pinto-Junior~~, V R~~]]	+	[[Category: Pinto-Junior VR]]
-	[[Category: Santiago~~, M Q~~]]	+	[[Category: Santiago MQ]]
-	[[Category: Silva-Filho~~, J C]]~~	+	[[Category: Silva-Filho JC]]
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 08:00, 29 April 2020

2020-04-29T08:00:53Z

←Older revision		Revision as of 08:00, 29 April 2020
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	==Crystal structure of Canavalia virosa lectin in complex with alpha-methyl-mannoside==		==Crystal structure of Canavalia virosa lectin in complex with alpha-methyl-mannoside==
-	<StructureSection load='5f5q' size='340' side='right' caption='[[5f5q]], [[Resolution\|resolution]] 2.52Å' scene=''>	+	<StructureSection load='5f5q' size='340' side='right'caption='[[5f5q]], [[Resolution\|resolution]] 2.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5f5q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_cathartica Canavalia cathartica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5Q OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5F5Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5f5q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_cathartica Canavalia cathartica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5F5Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5f5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5q OCA], [http://pdbe.org/5f5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f5q RCSB], [http://www.ebi.ac.uk/pdbsum/5f5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5f5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5q OCA], [http://pdbe.org/5f5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f5q RCSB], [http://www.ebi.ac.uk/pdbsum/5f5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Concanavalin A\|Concanavalin A]]	+	*[[Concanavalin 3D structures\|Concanavalin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Canavalia cathartica]]		[[Category: Canavalia cathartica]]
		+	[[Category: Large Structures]]
	[[Category: Cavada, B S]]		[[Category: Cavada, B S]]
	[[Category: Delatorre, P]]		[[Category: Delatorre, P]]

OCA at 12:19, 7 October 2017

2017-10-07T12:19:03Z

←Older revision		Revision as of 12:19, 7 October 2017
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5f5q" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5f5q" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Concanavalin A\|Concanavalin A]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:25, 26 October 2016

2016-10-26T18:25:44Z

←Older revision		Revision as of 18:25, 26 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5f5q is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of Canavalia virosa lectin in complex with alpha-methyl-mannoside==
		+	<StructureSection load='5f5q' size='340' side='right' caption='[[5f5q]], [[Resolution\|resolution]] 2.52Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5f5q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_cathartica Canavalia cathartica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F5Q FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5q OCA], [http://pdbe.org/5f5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f5q RCSB], [http://www.ebi.ac.uk/pdbsum/5f5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5q ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CONA_CANCT CONA_CANCT]] Glucose/D-mannose specific lectin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A lectin from Canavalia virosa, Diocleinae subtribe, was purified by affinity chromatography with Sephadex G-50 matrix and named ConV. The primary structure of ConV was obtained by mass spectrometry and crystals were obtained by the vapor diffusion method at 293K and belonged to orthorhombic space group P21221 with two molecules in its asymmetric unit. The structure obtained presented Rfactor and Rfree of 18.91% and 24.92% respectively, with no residues in nonallowed regions of Ramachandran plot. The crystal structure was solved at 2.53A and was demonstrated to be very similar to other lectins from the same subtribe. In inflammatory tests, ConV elicited paw edema, but incubation of lectin with glucose beforehand was able to reduce the edematogenic effect, indicating the involvement of the carbohydrate recognition domain in this process. The lectin also showed toxicity to rat C6 glioma cells, disrupting the mitochondrial membrane potential (DeltaYm) and decreasing cell viability, indicating an anticancer potential for ConV. In silico studies confirmed that ConV interacts strongly with carbohydrates that comprise the N-glycans of glycoproteins. This finding corroborates the hypothesis which holds that the lectin domain interacts with glycans in molecular targets and that this contributes to the effects observed in biological activities.

-	~~Authors:~~ Osterne~~, V.J.S.~~, Silva-Filho, ~~J.C.~~, Pinto-Junior, ~~V.R.~~, ~~Santiago~~, ~~M.Q.~~, ~~Lossio~~, ~~C.F.~~, Delatorre, P., Nascimento, K.S., ~~Cavada, B~~.S.	+	Structural characterization of a lectin from Canavalia virosa seeds with inflammatory and cytotoxic activities.,Osterne VJ, Silva-Filho JC, Santiago MQ, Pinto-Junior VR, Almeida AC, Barreto AA, Wolin IA, Nascimento AP, Amorim RM, Rocha BA, Delatorre P, Nagano CS, Leal RB, Assreuy AM, Nascimento KS, Cavada BS Int J Biol Macromol. 2016 Oct 11;94(Pt A):271-282. doi:, 10.1016/j.ijbiomac.2016.10.020. PMID:27737777<ref>PMID:27737777</ref>

-	~~Description: Crystal structure~~ of ~~Canavalia virosa lectin in complex with alpha~~-~~methyl~~-~~mannoside~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Lossio, C.F]]~~	+	<div class="pdbe-citations 5f5q" style="background-color:#fffaf0;"></div>
-	~~[[Category: Nascimento, K.S]]~~	+	== References ==
-	~~[[Category: Silva-Filho, J.C]]~~	+	<references/>
-	~~[[Category: Pinto-Junior, V.R]]~~	+	__TOC__
-	[[Category: ~~Santiago, M.Q]]~~	+	</StructureSection>
-	~~[[Category: Osterne, V.J.S~~]]	+	[[Category: Canavalia cathartica]]
-	[[Category: Cavada, B.S]]	+	[[Category: Cavada, B S]]
	[[Category: Delatorre, P]]		[[Category: Delatorre, P]]
		+	[[Category: Lossio, C F]]
		+	[[Category: Nascimento, K S]]
		+	[[Category: Osterne, V J.S]]
		+	[[Category: Pinto-Junior, V R]]
		+	[[Category: Santiago, M Q]]
		+	[[Category: Silva-Filho, J C]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Sugar binding protein]]

OCA at 17:53, 13 January 2016

2016-01-13T17:53:41Z

←Older revision		Revision as of 17:53, 13 January 2016
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	The entry 5f5q is ON HOLD until Paper Publication		The entry 5f5q is ON HOLD until Paper Publication

-	Authors: Osterne, V.J.S, Silva-Filho, J.C., Pinto-Junior, V.R., Santiago, M.Q., Lossio, C.F., Delatorre, P., Nascimento, K.S., Cavada, B.S.	+	Authors: Osterne, V.J.S., Silva-Filho, J.C., Pinto-Junior, V.R., Santiago, M.Q., Lossio, C.F., Delatorre, P., Nascimento, K.S., Cavada, B.S.

	Description: Crystal structure of Canavalia virosa lectin in complex with alpha-methyl-mannoside		Description: Crystal structure of Canavalia virosa lectin in complex with alpha-methyl-mannoside
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Lossio, C.F]]		[[Category: Lossio, C.F]]
-	[[Category: Osterne, V.J.S, Silva-Filho, J.C]]
	[[Category: Nascimento, K.S]]		[[Category: Nascimento, K.S]]
		+	[[Category: Silva-Filho, J.C]]
	[[Category: Pinto-Junior, V.R]]		[[Category: Pinto-Junior, V.R]]
	[[Category: Santiago, M.Q]]		[[Category: Santiago, M.Q]]
		+	[[Category: Osterne, V.J.S]]
	[[Category: Cavada, B.S]]		[[Category: Cavada, B.S]]
	[[Category: Delatorre, P]]		[[Category: Delatorre, P]]

OCA: Protected "5f5q" [edit=sysop:move=sysop]

2015-12-16T12:35:12Z

Protected "5f5q" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:35, 16 December 2015

OCA: New page: '''Unreleased structure''' The entry 5f5q is ON HOLD until Paper Publication Authors: Osterne, V.J.S, Silva-Filho, J.C., Pinto-Junior, V.R., Santiago, M.Q., Lossio, C.F., Delatorre, P.,...

2015-12-16T12:35:11Z

New page: '''Unreleased structure''' The entry 5f5q is ON HOLD until Paper Publication Authors: Osterne, V.J.S, Silva-Filho, J.C., Pinto-Junior, V.R., Santiago, M.Q., Lossio, C.F., Delatorre, P.,...

New page

'''Unreleased structure'''

The entry 5f5q is ON HOLD until Paper Publication

Authors: Osterne, V.J.S, Silva-Filho, J.C., Pinto-Junior, V.R., Santiago, M.Q., Lossio, C.F., Delatorre, P., Nascimento, K.S., Cavada, B.S.

Description: Crystal structure of Canavalia virosa lectin in complex with alpha-methyl-mannoside
[[Category: Unreleased Structures]]
[[Category: Lossio, C.F]]
[[Category: Osterne, V.J.S, Silva-Filho, J.C]]
[[Category: Nascimento, K.S]]
[[Category: Pinto-Junior, V.R]]
[[Category: Santiago, M.Q]]
[[Category: Cavada, B.S]]
[[Category: Delatorre, P]]