5fbt - Revision history

OCA at 06:45, 19 July 2023

2023-07-19T06:45:41Z

←Older revision		Revision as of 06:45, 19 July 2023
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	<StructureSection load='5fbt' size='340' side='right'caption='[[5fbt]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5fbt' size='340' side='right'caption='[[5fbt]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fbt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lismf Lismf~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FBT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fbt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_serotype_4b_str._F2365 Listeria monocytogenes serotype 4b str. F2365]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FBT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=5WQ:RIFAMPIN'>5WQ</scene>~~, ~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.702Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5WQ:RIFAMPIN'>5WQ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat">[[5fbs\|5fbs]]~~, ~~[[5fbu\|5fbu]]~~<~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbt OCA], [https://pdbe.org/5fbt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fbt RCSB], [https://www.ebi.ac.uk/pdbsum/5fbt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fbt ProSAT]</span></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">LmNIHS28_01948 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=265669 LISMF])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbt OCA], [~~http~~://pdbe.org/5fbt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fbt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fbt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fbt ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0X1KHF9_LISMF A0A0X1KHF9_LISMF]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lismf~~]]	+	[[Category: Listeria monocytogenes serotype 4b str. F2365]]
-	[[Category: Anderson~~, W F]]~~	+	[[Category: Anderson WF]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Savchenko A]]
-	[[Category: Savchenko, A]]	+	[[Category: Skarina T]]
-	[[Category: Skarina, T]]	+	[[Category: Stogios PJ]]
-	[[Category: Stogios~~, P J~~]]	+	[[Category: Wawrzak Z]]
-	[[Category: Wawrzak, Z]]	+	[[Category: Yim V]]
-	[[Category: Yim, V]]	+
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Atp grasp domain]]~~	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Phosphohistidine domain]]~~	+
-	~~[[Category: Phosphotransferase]]~~	+
-	~~[[Category: Rifampin]]~~	+
-	~~[[Category: Rifamycin]]~~	+
-	~~[[Category: Transferase-antibiotic complex~~]]	+

OCA at 08:09, 29 April 2020

2020-04-29T08:09:36Z

←Older revision		Revision as of 08:09, 29 April 2020
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	==Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with rifampin==		==Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with rifampin==
-	<StructureSection load='5fbt' size='340' side='right' caption='[[5fbt]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='5fbt' size='340' side='right'caption='[[5fbt]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fbt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBT OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FBT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fbt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lismf Lismf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FBT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5WQ:RIFAMPIN'>5WQ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5WQ:RIFAMPIN'>5WQ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fbs\|5fbs]], [[5fbu\|5fbu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fbs\|5fbs]], [[5fbu\|5fbu]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbt OCA], [http://pdbe.org/5fbt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fbt RCSB], [http://www.ebi.ac.uk/pdbsum/5fbt PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LmNIHS28_01948 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=265669 LISMF])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbt OCA], [http://pdbe.org/5fbt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fbt RCSB], [http://www.ebi.ac.uk/pdbsum/5fbt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fbt ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lismf]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 18:02, 10 May 2016

2016-05-10T18:02:18Z

←Older revision		Revision as of 18:02, 10 May 2016
Line 1:		Line 1:
		+
	==Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with rifampin==		==Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with rifampin==
	<StructureSection load='5fbt' size='340' side='right' caption='[[5fbt]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5fbt' size='340' side='right' caption='[[5fbt]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 8:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbt OCA], [http://pdbe.org/5fbt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fbt RCSB], [http://www.ebi.ac.uk/pdbsum/5fbt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbt OCA], [http://pdbe.org/5fbt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fbt RCSB], [http://www.ebi.ac.uk/pdbsum/5fbt PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rifampin (RIF) phosphotransferase (RPH) confers antibiotic resistance by conversion of RIF and ATP, to inactive phospho-RIF, AMP and Pi. Here we present the crystal structure of RPH from Listeria monocytogenes (RPH-Lm), which reveals that the enzyme is comprised of three domains: two substrate-binding domains (ATP-grasp and RIF-binding domains); and a smaller phosphate-carrying His swivel domain. Using solution small-angle X-ray scattering and mutagenesis, we reveal a mechanism where the swivel domain transits between the spatially distinct substrate-binding sites during catalysis. RPHs are previously uncharacterized dikinases that are widespread in environmental and pathogenic bacteria. These enzymes are members of a large unexplored group of bacterial enzymes with substrate affinities that have yet to be fully explored. Such an enzymatically complex mechanism of antibiotic resistance augments the spectrum of strategies used by bacteria to evade antimicrobial compounds.
		+
		+	Rifampin phosphotransferase is an unusual antibiotic resistance kinase.,Stogios PJ, Cox G, Spanogiannopoulos P, Pillon MC, Waglechner N, Skarina T, Koteva K, Guarne A, Savchenko A, Wright GD Nat Commun. 2016 Apr 22;7:11343. doi: 10.1038/ncomms11343. PMID:27103605<ref>PMID:27103605</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5fbt" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
Line 15:		Line 27:
	[[Category: Skarina, T]]		[[Category: Skarina, T]]
	[[Category: Stogios, P J]]		[[Category: Stogios, P J]]
		+	[[Category: Wawrzak, Z]]
	[[Category: Yim, V]]		[[Category: Yim, V]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]

OCA at 19:40, 30 December 2015

2015-12-30T19:40:59Z

←Older revision		Revision as of 19:40, 30 December 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with rifampin==
-		+	<StructureSection load='5fbt' size='340' side='right' caption='[[5fbt]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~The entry~~ 5fbt is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5fbt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FBT FirstGlance]. <br>
-	~~Authors~~: ~~Stogios~~, P.J.~~, Skarina, T~~., ~~Yim, V~~., ~~Savchenko, A~~., ~~Anderson, W~~.F., ~~Center for Structural Genomics of Infectious Diseases (CSGID)~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5WQ:RIFAMPIN'>5WQ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~Description~~: ~~Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with rifampin~~	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fbs\|5fbs]], [[5fbu\|5fbu]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbt OCA], [http://pdbe.org/5fbt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fbt RCSB], [http://www.ebi.ac.uk/pdbsum/5fbt PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Anderson, W F]]
		+	[[Category: Structural genomic]]
	[[Category: Savchenko, A]]		[[Category: Savchenko, A]]
-	[[Category: Yim, V]]
-	[[Category: Stogios, P.J]]
	[[Category: Skarina, T]]		[[Category: Skarina, T]]
-	[[Category: ~~Anderson~~, ~~W.F~~]]	+	[[Category: Stogios, P J]]
-	[[Category: ~~Center For Structural Genomics Of Infectious Diseases (~~Csgid)]]	+	[[Category: Yim, V]]
		+	[[Category: Antibiotic resistance]]
		+	[[Category: Atp grasp domain]]
		+	[[Category: Csgid]]
		+	[[Category: Phosphohistidine domain]]
		+	[[Category: Phosphotransferase]]
		+	[[Category: Rifampin]]
		+	[[Category: Rifamycin]]
		+	[[Category: Transferase-antibiotic complex]]

OCA: Protected "5fbt" [edit=sysop:move=sysop]

2015-12-23T11:25:28Z

Protected "5fbt" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:25, 23 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fbt is ON HOLD Authors: Stogios, P.J., Skarina, T., Yim, V., Savchenko, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (...

2015-12-23T11:25:27Z

New page: '''Unreleased structure''' The entry 5fbt is ON HOLD Authors: Stogios, P.J., Skarina, T., Yim, V., Savchenko, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (...

New page

'''Unreleased structure'''

The entry 5fbt is ON HOLD

Authors: Stogios, P.J., Skarina, T., Yim, V., Savchenko, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with rifampin
[[Category: Unreleased Structures]]
[[Category: Savchenko, A]]
[[Category: Yim, V]]
[[Category: Stogios, P.J]]
[[Category: Skarina, T]]
[[Category: Anderson, W.F]]
[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]