5fby - Revision history

OCA at 12:30, 6 March 2024

2024-03-06T12:30:56Z

←Older revision		Revision as of 12:30, 6 March 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/G0SHM3_CHATD G0SHM3_CHATD]		[https://www.uniprot.org/uniprot/G0SHM3_CHATD G0SHM3_CHATD]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Accurate chromosome segregation requires timely dissolution of chromosome cohesion after chromosomes are properly attached to the mitotic spindle. Separase is absolutely essential for cohesion dissolution in organisms from yeast to man. It cleaves the kleisin subunit of cohesin and opens the cohesin ring to allow chromosome segregation. Cohesin cleavage is spatiotemporally controlled by separase-associated regulatory proteins, including the inhibitory chaperone securin, and by phosphorylation of both the enzyme and substrates. Dysregulation of this process causes chromosome missegregation and aneuploidy, contributing to cancer and birth defects. Despite its essential functions, atomic structures of separase have not been determined. Here we report crystal structures of the separase protease domain from the thermophilic fungus Chaetomium thermophilum, alone or covalently bound to unphosphorylated and phosphorylated inhibitory peptides derived from a cohesin cleavage site. These structures reveal how separase recognizes cohesin and how cohesin phosphorylation by polo-like kinase 1 (Plk1) enhances cleavage. Consistent with a previous cellular study, mutating two securin residues in a conserved motif that partly matches the separase cleavage consensus converts securin from a separase inhibitor to a substrate. Our study establishes atomic mechanisms of substrate cleavage by separase and suggests competitive inhibition by securin.
-
-	Structural basis of cohesin cleavage by separase.,Lin Z, Luo X, Yu H Nature. 2016 Apr 7;532(7597):131-4. doi: 10.1038/nature17402. Epub 2016 Mar 30. PMID:27027290<ref>PMID:27027290</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5fby" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:45, 19 July 2023

2023-07-19T06:45:50Z

←Older revision		Revision as of 06:45, 19 July 2023
Line 1:		Line 1:

	==Crystal structure of ctSPD==		==Crystal structure of ctSPD==
-	<StructureSection load='5fby' size='340' side='right' caption='[[5fby]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5fby' size='340' side='right'caption='[[5fby]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fby]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chatd Chatd~~] and [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FBY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fby]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FBY FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5fc2\|5fc2]], [[5fc3\|5fc3]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.898Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">CTHT_0070540 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=759272 CHATD])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fby OCA], [https://pdbe.org/5fby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fby RCSB], [https://www.ebi.ac.uk/pdbsum/5fby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fby ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fby OCA], [~~http~~://pdbe.org/5fby PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fby RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fby PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fby ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G0SHM3_CHATD G0SHM3_CHATD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chatd~~]]	+	[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Large Structures]]
-	[[Category: Lin, Z]]	+	[[Category: Synthetic construct]]
-	[[Category: Luo, X]]	+	[[Category: Lin Z]]
-	[[Category: Yu, H]]	+	[[Category: Luo X]]
-	~~[[Category: Cohesin]]~~	+	[[Category: Yu H]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 12:42, 6 November 2017

2017-11-06T12:42:12Z

←Older revision		Revision as of 12:42, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='5fby' size='340' side='right' caption='[[5fby]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5fby' size='340' side='right' caption='[[5fby]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fby]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FBY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fby]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FBY FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fc2\|5fc2]], [[5fc3\|5fc3]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fc2\|5fc2]], [[5fc3\|5fc3]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fby OCA], [http://pdbe.org/5fby PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fby RCSB], [http://www.ebi.ac.uk/pdbsum/5fby PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTHT_0070540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fby OCA], [http://pdbe.org/5fby PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fby RCSB], [http://www.ebi.ac.uk/pdbsum/5fby PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fby ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chatd]]
		+	[[Category: Synthetic construct sequences]]
	[[Category: Lin, Z]]		[[Category: Lin, Z]]
	[[Category: Luo, X]]		[[Category: Luo, X]]

OCA at 17:00, 10 May 2016

2016-05-10T17:00:05Z

←Older revision		Revision as of 17:00, 10 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5fby is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of ctSPD==
		+	<StructureSection load='5fby' size='340' side='right' caption='[[5fby]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5fby]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FBY FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fc2\|5fc2]], [[5fc3\|5fc3]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fby OCA], [http://pdbe.org/5fby PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fby RCSB], [http://www.ebi.ac.uk/pdbsum/5fby PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Accurate chromosome segregation requires timely dissolution of chromosome cohesion after chromosomes are properly attached to the mitotic spindle. Separase is absolutely essential for cohesion dissolution in organisms from yeast to man. It cleaves the kleisin subunit of cohesin and opens the cohesin ring to allow chromosome segregation. Cohesin cleavage is spatiotemporally controlled by separase-associated regulatory proteins, including the inhibitory chaperone securin, and by phosphorylation of both the enzyme and substrates. Dysregulation of this process causes chromosome missegregation and aneuploidy, contributing to cancer and birth defects. Despite its essential functions, atomic structures of separase have not been determined. Here we report crystal structures of the separase protease domain from the thermophilic fungus Chaetomium thermophilum, alone or covalently bound to unphosphorylated and phosphorylated inhibitory peptides derived from a cohesin cleavage site. These structures reveal how separase recognizes cohesin and how cohesin phosphorylation by polo-like kinase 1 (Plk1) enhances cleavage. Consistent with a previous cellular study, mutating two securin residues in a conserved motif that partly matches the separase cleavage consensus converts securin from a separase inhibitor to a substrate. Our study establishes atomic mechanisms of substrate cleavage by separase and suggests competitive inhibition by securin.

-	~~Authors: Lin~~, Z., Luo, X., Yu, H.	+	Structural basis of cohesin cleavage by separase.,Lin Z, Luo X, Yu H Nature. 2016 Apr 7;532(7597):131-4. doi: 10.1038/nature17402. Epub 2016 Mar 30. PMID:27027290<ref>PMID:27027290</ref>

-	~~Description:~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Yu, H]]~~	+	<div class="pdbe-citations 5fby" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Lin, Z]]		[[Category: Lin, Z]]
	[[Category: Luo, X]]		[[Category: Luo, X]]
		+	[[Category: Yu, H]]
		+	[[Category: Cohesin]]
		+	[[Category: Complex]]
		+	[[Category: Hydrolase]]

OCA at 10:53, 7 January 2016

2016-01-07T10:53:20Z

←Older revision		Revision as of 10:53, 7 January 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5fby is ON HOLD	+	The entry 5fby is ON HOLD until Paper Publication

	Authors: Lin, Z., Luo, X., Yu, H.		Authors: Lin, Z., Luo, X., Yu, H.

OCA: Protected "5fby" [edit=sysop:move=sysop]

2015-12-23T11:25:48Z

Protected "5fby" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:25, 23 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fby is ON HOLD Authors: Lin, Z., Luo, X., Yu, H. Description: Category: Unreleased Structures Category: Yu, H Category: Lin, Z [[Cate...

2015-12-23T11:25:47Z

New page: '''Unreleased structure''' The entry 5fby is ON HOLD Authors: Lin, Z., Luo, X., Yu, H. Description: Category: Unreleased Structures Category: Yu, H Category: Lin, Z [[Cate...

New page

'''Unreleased structure'''

The entry 5fby is ON HOLD

Authors: Lin, Z., Luo, X., Yu, H.

Description:
[[Category: Unreleased Structures]]
[[Category: Yu, H]]
[[Category: Lin, Z]]
[[Category: Luo, X]]