5ffq - Revision history

OCA at 06:49, 19 July 2023

2023-07-19T06:49:33Z

←Older revision		Revision as of 06:49, 19 July 2023
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	<StructureSection load='5ffq' size='340' side='right'caption='[[5ffq]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5ffq' size='340' side='right'caption='[[5ffq]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ffq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FFQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FFQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ffq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FFQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ffq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ffq OCA], [~~http~~://pdbe.org/5ffq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ffq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ffq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ffq ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ffq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ffq OCA], [https://pdbe.org/5ffq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ffq RCSB], [https://www.ebi.ac.uk/pdbsum/5ffq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ffq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8X5N4_ECO57 Q8X5N4_ECO57]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli O157:H7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: LaMattina~~, J W~~]]	+	[[Category: LaMattina JW]]
-	[[Category: Lanzilotta~~, W N~~]]	+	[[Category: Lanzilotta WN]]
-	[[Category: Reedy~~, A N~~]]	+	[[Category: Reedy AN]]
-	[[Category: Uy~~, K G]]~~	+	[[Category: Uy KG]]
-	~~[[Category: Nadph oxidoreductase]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 07:17, 6 May 2020

2020-05-06T07:17:07Z

←Older revision		Revision as of 07:17, 6 May 2020
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	==ChuY: An Anaerobillin Reductase from Escherichia coli O157:H7==		==ChuY: An Anaerobillin Reductase from Escherichia coli O157:H7==
-	<StructureSection load='5ffq' size='340' side='right' caption='[[5ffq]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5ffq' size='340' side='right'caption='[[5ffq]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ffq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FFQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FFQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ffq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FFQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FFQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ffq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ffq OCA], [http://pdbe.org/5ffq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ffq RCSB], [http://www.ebi.ac.uk/pdbsum/5ffq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ffq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ffq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ffq OCA], [http://pdbe.org/5ffq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ffq RCSB], [http://www.ebi.ac.uk/pdbsum/5ffq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ffq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: LaMattina, J W]]		[[Category: LaMattina, J W]]
	[[Category: Lanzilotta, W N]]		[[Category: Lanzilotta, W N]]

OCA at 09:17, 4 October 2017

2017-10-04T09:17:55Z

←Older revision		Revision as of 09:17, 4 October 2017
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ffq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ffq OCA], [http://pdbe.org/5ffq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ffq RCSB], [http://www.ebi.ac.uk/pdbsum/5ffq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ffq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ffq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ffq OCA], [http://pdbe.org/5ffq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ffq RCSB], [http://www.ebi.ac.uk/pdbsum/5ffq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ffq ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	All of the heme-degrading enzymes that have been characterized to date require molecular oxygen as a cosubstrate. Escherichia coli O157:H7 has been shown to express heme uptake and transport proteins, as well as use heme as an iron source. This enteric pathogen colonizes the anaerobic space of the lower intestine in mammals, yet no mechanism for anaerobic heme degradation has been reported. Herein we provide evidence for an oxygen-independent heme-degradation pathway. Specifically, we demonstrate that ChuW is a radical S-adenosylmethionine methyltransferase that catalyzes a radical-mediated mechanism facilitating iron liberation and the production of the tetrapyrrole product we termed "anaerobilin." We further demonstrate that anaerobilin can be used as a substrate by ChuY, an enzyme that is coexpressed with ChuW in vivo along with the heme uptake machinery. Our findings are discussed in terms of the competitive advantage this system provides for enteric bacteria, particularly those that inhabit an anaerobic niche in the intestines.
		+
		+	Radical new paradigm for heme degradation in Escherichia coli O157:H7.,LaMattina JW, Nix DB, Lanzilotta WN Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12138-12143. Epub 2016 Oct 10. PMID:27791000<ref>PMID:27791000</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ffq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: LaMattina, J L]]	+	[[Category: LaMattina, J W]]
	[[Category: Lanzilotta, W N]]		[[Category: Lanzilotta, W N]]
	[[Category: Reedy, A N]]		[[Category: Reedy, A N]]

OCA at 16:24, 18 January 2017

2017-01-18T16:24:57Z

←Older revision		Revision as of 16:24, 18 January 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5ffq ~~is ON HOLD until Paper Publication~~	+	==ChuY: An Anaerobillin Reductase from Escherichia coli O157:H7==
-		+	<StructureSection load='5ffq' size='340' side='right' caption='[[5ffq]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors~~: ~~LaMattina, J~~.L.~~, Reedy, A~~.N.~~, Uy, K~~.G.~~, Lanzilotta, W~~.N.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ffq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FFQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FFQ FirstGlance]. <br>
-	~~Description~~: ~~ChuY~~: ~~An Anaerobillin Reductase from Escherichia coli O157~~:H7	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ffq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ffq OCA], [http://pdbe.org/5ffq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ffq RCSB], [http://www.ebi.ac.uk/pdbsum/5ffq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ffq ProSAT]</span></td></tr>
-	[[Category: Uy, ~~K.G~~]]	+	</table>
-	[[Category: Lanzilotta, W.N]]	+	__TOC__
-	[[Category: Reedy, A.N]]	+	</StructureSection>
-	[[Category: ~~Lamattina~~, ~~J.L~~]]	+	[[Category: LaMattina, J L]]
		+	[[Category: Lanzilotta, W N]]
		+	[[Category: Reedy, A N]]
		+	[[Category: Uy, K G]]
		+	[[Category: Nadph oxidoreductase]]
		+	[[Category: Unknown function]]

OCA at 14:29, 10 February 2016

2016-02-10T14:29:13Z

←Older revision		Revision as of 14:29, 10 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ffq is ON HOLD	+	The entry 5ffq is ON HOLD until Paper Publication

	Authors: LaMattina, J.L., Reedy, A.N., Uy, K.G., Lanzilotta, W.N.		Authors: LaMattina, J.L., Reedy, A.N., Uy, K.G., Lanzilotta, W.N.

OCA at 18:12, 13 January 2016

2016-01-13T18:12:04Z

←Older revision		Revision as of 18:12, 13 January 2016
Line 3:		Line 3:
	The entry 5ffq is ON HOLD		The entry 5ffq is ON HOLD

-	Authors: LaMattina, J.L.; Reedy, A.N.; Uy, K.G.~~; and~~ Lanzilotta, W.N.	+	Authors: LaMattina, J.L., Reedy, A.N., Uy, K.G., Lanzilotta, W.N.

	Description: ChuY: An Anaerobillin Reductase from Escherichia coli O157:H7		Description: ChuY: An Anaerobillin Reductase from Escherichia coli O157:H7
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Lamattina~~, J.L.; Reedy, A.N~~.; Uy~~, K.~~G.; And Lanzilotta, W.N~~]]	+	[[Category: Uy, K.G]]
		+	[[Category: Lanzilotta, W.N]]
		+	[[Category: Reedy, A.N]]
		+	[[Category: Lamattina, J.L]]

OCA: Protected "5ffq" [edit=sysop:move=sysop]

2015-12-30T17:53:19Z

Protected "5ffq" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:53, 30 December 2015

OCA: New page: '''Unreleased structure''' The entry 5ffq is ON HOLD Authors: LaMattina, J.L.; Reedy, A.N.; Uy, K.G.; and Lanzilotta, W.N. Description: ChuY: An Anaerobillin Reductase from Escherichia...

2015-12-30T17:53:18Z

New page: '''Unreleased structure''' The entry 5ffq is ON HOLD Authors: LaMattina, J.L.; Reedy, A.N.; Uy, K.G.; and Lanzilotta, W.N. Description: ChuY: An Anaerobillin Reductase from Escherichia...

New page

'''Unreleased structure'''

The entry 5ffq is ON HOLD

Authors: LaMattina, J.L.; Reedy, A.N.; Uy, K.G.; and Lanzilotta, W.N.

Description: ChuY: An Anaerobillin Reductase from Escherichia coli O157:H7
[[Category: Unreleased Structures]]
[[Category: Lamattina, J.L.; Reedy, A.N.; Uy, K.G.; And Lanzilotta, W.N]]