5fkl - Revision history

OCA at 06:55, 19 July 2023

2023-07-19T06:55:21Z

←Older revision		Revision as of 06:55, 19 July 2023
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	<StructureSection load='5fkl' size='340' side='right'caption='[[5fkl]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5fkl' size='340' side='right'caption='[[5fkl]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fkl]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FKL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fkl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FKL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fkk\|5fkk]], [[5fkm\|5fkm]], [[5fkn\|5fkn]], [[5fko\|5fko]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkl OCA], [~~http~~://pdbe.org/5fkl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fkl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fkl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fkl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkl OCA], [https://pdbe.org/5fkl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fkl RCSB], [https://www.ebi.ac.uk/pdbsum/5fkl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fkl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TETR4_ECOLX TETR4_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:

	==See Also==		==See Also==
-	*[[Tetracycline repressor protein\|Tetracycline repressor protein]]	+	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hinrichs, W]]	+	[[Category: Hinrichs W]]
-	[[Category: Palm~~, G J~~]]	+	[[Category: Palm GJ]]
-	[[Category: Schneider, J]]	+	[[Category: Schneider J]]
-	[[Category: Werten, S]]	+	[[Category: Werten S]]
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Repressor]]~~	+
-	~~[[Category: Tetr]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 12:59, 10 May 2019

2019-05-10T12:59:02Z

←Older revision		Revision as of 12:59, 10 May 2019
Line 1:		Line 1:

	==TetR(D) H100A mutant in complex with anhydrotetracycline and magnesium==		==TetR(D) H100A mutant in complex with anhydrotetracycline and magnesium==
-	<StructureSection load='5fkl' size='340' side='right' caption='[[5fkl]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5fkl' size='340' side='right'caption='[[5fkl]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fkl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FKL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fkl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FKL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fkk\|5fkk]], [[5fkm\|5fkm]], [[5fkn\|5fkn]], [[5fko\|5fko]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fkk\|5fkk]], [[5fkm\|5fkm]], [[5fkn\|5fkn]], [[5fko\|5fko]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkl OCA], [http://pdbe.org/5fkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fkl RCSB], [http://www.ebi.ac.uk/pdbsum/5fkl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkl OCA], [http://pdbe.org/5fkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fkl RCSB], [http://www.ebi.ac.uk/pdbsum/5fkl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fkl ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 5fkl" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5fkl" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tetracycline repressor protein\|Tetracycline repressor protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Hinrichs, W]]		[[Category: Hinrichs, W]]
	[[Category: Palm, G J]]		[[Category: Palm, G J]]

OCA at 15:57, 10 May 2016

2016-05-10T15:57:38Z

←Older revision		Revision as of 15:57, 10 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5fkl is ~~ON HOLD until Paper~~ Publication	+	==TetR(D) H100A mutant in complex with anhydrotetracycline and magnesium==
		+	<StructureSection load='5fkl' size='340' side='right' caption='[[5fkl]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5fkl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FKL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fkk\|5fkk]], [[5fkm\|5fkm]], [[5fkn\|5fkn]], [[5fko\|5fko]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkl OCA], [http://pdbe.org/5fkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fkl RCSB], [http://www.ebi.ac.uk/pdbsum/5fkl PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Induction of the tetracycline repressor (TetR) results from antibiotic-dependent changes in the relative positioning of the DNA-binding domains within the promoter-associated repressor dimer, but the key determinants of this allosteric effect remain poorly characterised. Intriguingly, previous mutational analyses of the tetracycline-interacting site revealed a lack of correlation between residual affinity and induction propensity, suggesting that some of the residues in contact with the antibiotic primarily act in ligand recognition and retention, whereas others are required to transmit the allosteric signal. Here, we provide a structural basis for these observations via crystallographic analysis of the point mutants N82A, H100A, T103A and E147A in complex with the inducer 5a,6-anhydrotetracycline. In conjunction with the available functional data, the four structures demonstrate that a trigger-like movement of the region between helices alpha6 and alpha7 towards and into the binding site plays a decisive role in the intramolecular communication process. In sharp contrast, residues lining the binding cavity proper have little or no influence on the allosteric mechanism as such. This nearly complete physical separation of ligand recognition and allostery will have allowed diverging TetR-like repressors to bind novel effectors while the existing induction mechanism remained intact. Consequently, the modularity described here may have been a key factor in the evolutionary success of the widespread and highly diversified repressor class. DATABASE: Structural data are available in the Protein Data Bank under the accession numbers 5FKK, 5FKL, 5FKM, 5FKN and 5FKO.

-	~~Authors: Werten~~, S., Schneider, J., Palm~~, G.J.~~, Hinrichs, W.	+	Modular organisation of inducer recognition and allostery in the tetracycline repressor.,Werten S, Schneider J, Palm GJ, Hinrichs W FEBS J. 2016 Mar 30. doi: 10.1111/febs.13723. PMID:27028290<ref>PMID:27028290</ref>

-	~~Description: TetR(D) H100A mutant in complex with anhydrotetracycline and magnesium~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5fkl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Hinrichs, W]]		[[Category: Hinrichs, W]]
-	[[Category: Palm, G.J]]	+	[[Category: Palm, G J]]
	[[Category: Schneider, J]]		[[Category: Schneider, J]]
	[[Category: Werten, S]]		[[Category: Werten, S]]
		+	[[Category: Antibiotic resistance]]
		+	[[Category: Repressor]]
		+	[[Category: Tetr]]
		+	[[Category: Transcription]]

OCA: Protected "5fkl" [edit=sysop:move=sysop]

2015-12-01T03:31:02Z

Protected "5fkl" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:31, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fkl is ON HOLD until Paper Publication Authors: Werten, S., Schneider, J., Palm, G.J., Hinrichs, W. Description: TetR(D) H100A mutant in complex w...

2015-12-01T03:31:01Z

New page: '''Unreleased structure''' The entry 5fkl is ON HOLD until Paper Publication Authors: Werten, S., Schneider, J., Palm, G.J., Hinrichs, W. Description: TetR(D) H100A mutant in complex w...

New page

'''Unreleased structure'''

The entry 5fkl is ON HOLD until Paper Publication

Authors: Werten, S., Schneider, J., Palm, G.J., Hinrichs, W.

Description: TetR(D) H100A mutant in complex with anhydrotetracycline and magnesium
[[Category: Unreleased Structures]]
[[Category: Hinrichs, W]]
[[Category: Palm, G.J]]
[[Category: Schneider, J]]
[[Category: Werten, S]]