5fnx - Revision history

OCA at 07:00, 19 July 2023

2023-07-19T07:00:31Z

←Older revision		Revision as of 07:00, 19 July 2023
Line 3:		Line 3:
	<StructureSection load='5fnx' size='340' side='right'caption='[[5fnx]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='5fnx' size='340' side='right'caption='[[5fnx]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fnx]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Potato Potato~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FNX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fnx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FNX FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">[[~~5fnw~~\|~~5fnw~~]]</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnx OCA], [~~http~~://pdbe.org/5fnx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fnx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fnx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnx OCA], [https://pdbe.org/5fnx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fnx RCSB], [https://www.ebi.ac.uk/pdbsum/5fnx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/M1AKE5_SOLTU M1AKE5_SOLTU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Potato~~]]	+	[[Category: Solanum tuberosum]]
-	[[Category: Guerra, Y]]	+	[[Category: Guerra Y]]
-	[[Category: Rudino-Pinera, E]]	+	[[Category: Rudino-Pinera E]]
-	~~[[Category: Aspartic protease]]~~	+
-	~~[[Category: Bi-functional protease inhibitor]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase inhibitor]]~~	+
-	~~[[Category: Kunitz-type inhibitor]]~~	+
-	~~[[Category: Protease inhibitor]]~~	+
-	~~[[Category: Serine protease]]~~	+
-	~~[[Category: Sti-kunitz inhibitor~~]]	+

OCA at 07:27, 6 May 2020

2020-05-06T07:27:36Z

←Older revision		Revision as of 07:27, 6 May 2020
Line 1:		Line 1:

	==Crystal structure at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of serine and aspartic proteases in space group p4322 and ph 9.0==		==Crystal structure at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of serine and aspartic proteases in space group p4322 and ph 9.0==
-	<StructureSection load='5fnx' size='340' side='right' caption='[[5fnx]], [[Resolution\|resolution]] 2.65Å' scene=''>	+	<StructureSection load='5fnx' size='340' side='right'caption='[[5fnx]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fnx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FNX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fnx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Potato Potato]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FNX FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fnw\|5fnw]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fnw\|5fnw]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnx OCA], [http://pdbe.org/5fnx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fnx RCSB], [http://www.ebi.ac.uk/pdbsum/5fnx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnx OCA], [http://pdbe.org/5fnx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fnx RCSB], [http://www.ebi.ac.uk/pdbsum/5fnx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Potato]]
	[[Category: Guerra, Y]]		[[Category: Guerra, Y]]
	[[Category: Rudino-Pinera, E]]		[[Category: Rudino-Pinera, E]]

OCA at 07:02, 29 March 2017

2017-03-29T07:02:31Z

←Older revision		Revision as of 07:02, 29 March 2017
Line 1:		Line 1:

-	==Crystal ~~structural~~ at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of serine and aspartic proteases in space group p4322 and ph 9.0==	+	==Crystal structure at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of serine and aspartic proteases in space group p4322 and ph 9.0==
	<StructureSection load='5fnx' size='340' side='right' caption='[[5fnx]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='5fnx' size='340' side='right' caption='[[5fnx]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 02:21, 13 July 2016

2016-07-13T02:21:24Z

←Older revision		Revision as of 02:21, 13 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5fnx is ~~ON HOLD until Paper~~ Publication	+	==Crystal structural at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of serine and aspartic proteases in space group p4322 and ph 9.0==
		+	<StructureSection load='5fnx' size='340' side='right' caption='[[5fnx]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5fnx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FNX FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fnw\|5fnw]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnx OCA], [http://pdbe.org/5fnx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fnx RCSB], [http://www.ebi.ac.uk/pdbsum/5fnx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnx ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bi-functional inhibitors from the Kunitz-type soybean trypsin inhibitor (STI) family are glycosylated proteins able to inhibit serine and aspartic proteases. Here we report six crystal structures of the wild-type and a non-glycosylated mutant of the bifunctional inhibitor E3Ad obtained at different pH values and space groups. The crystal structures show that E3Ad adopts the typical beta-trefoil fold of the STI family exhibiting some conformational changes due to pH variations and crystal packing. Despite the high sequence identity with a recently reported potato cathepsin D inhibitor (PDI), three-dimensional structures obtained in this work show a significant conformational change in the protease-binding loop proposed for aspartic protease inhibition. The E3Ad binding loop for serine protease inhibition is also proposed, based on structural similarity with a novel non-canonical conformation described for the double-headed inhibitor API-A from the Kunitz-type STI family. In addition, structural and sequence analyses suggest that bifunctional inhibitors of serine and aspartic proteases from the Kunitz-type STI family are more similar to double-headed inhibitor API-A than other inhibitors with a canonical protease-binding loop.

-	~~Authors~~: ~~Guerra~~, Y., Rudino-Pinera, E.	+	Structures of a bi-functional Kunitz-type STI family inhibitor of serine and aspartic proteases: Could the aspartic protease inhibition have evolved from a canonical serine protease-binding loop?,Guerra Y, Valiente PA, Pons T, Berry C, Rudino-Pinera E J Struct Biol. 2016 Aug;195(2):259-71. doi: 10.1016/j.jsb.2016.06.014. Epub 2016 , Jun 18. PMID:27329566<ref>PMID:27329566</ref>

-	~~Description: Crystal structural at pH 9~~.0 of ~~a potato STI~~-~~Kunitz bi~~-~~functional inhibitor of serine and aspartic proteases~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Rudino-Pinera, E]]~~	+	<div class="pdbe-citations 5fnx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Guerra, Y]]		[[Category: Guerra, Y]]
		+	[[Category: Rudino-Pinera, E]]
		+	[[Category: Aspartic protease]]
		+	[[Category: Bi-functional protease inhibitor]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase inhibitor]]
		+	[[Category: Kunitz-type inhibitor]]
		+	[[Category: Protease inhibitor]]
		+	[[Category: Serine protease]]
		+	[[Category: Sti-kunitz inhibitor]]

OCA at 11:43, 23 December 2015

2015-12-23T11:43:54Z

←Older revision		Revision as of 11:43, 23 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5fnx is ON HOLD	+	The entry 5fnx is ON HOLD until Paper Publication

	Authors: Guerra, Y., Rudino-Pinera, E.		Authors: Guerra, Y., Rudino-Pinera, E.

-	Description: Crystal ~~structure~~ at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of serine and aspartic proteases	+	Description: Crystal structural at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of serine and aspartic proteases
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Rudino-Pinera, E]]		[[Category: Rudino-Pinera, E]]
	[[Category: Guerra, Y]]		[[Category: Guerra, Y]]

OCA at 12:57, 16 December 2015

2015-12-16T12:57:10Z

←Older revision		Revision as of 12:57, 16 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5fnx is ON HOLD ~~until Paper Publication~~	+	The entry 5fnx is ON HOLD

	Authors: Guerra, Y., Rudino-Pinera, E.		Authors: Guerra, Y., Rudino-Pinera, E.

OCA at 15:22, 2 December 2015

2015-12-02T15:22:42Z

←Older revision		Revision as of 15:22, 2 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5fnx is ON HOLD	+	The entry 5fnx is ON HOLD until Paper Publication

	Authors: Guerra, Y., Rudino-Pinera, E.		Authors: Guerra, Y., Rudino-Pinera, E.

-	Description: Crystal ~~structural~~ at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of serine and aspartic proteases	+	Description: Crystal structure at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of serine and aspartic proteases
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Rudino-Pinera, E]]		[[Category: Rudino-Pinera, E]]
	[[Category: Guerra, Y]]		[[Category: Guerra, Y]]

OCA: Protected "5fnx" [edit=sysop:move=sysop]

2015-12-01T03:42:58Z

Protected "5fnx" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:42, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fnx is ON HOLD Authors: Guerra, Y., Rudino-Pinera, E. Description: Crystal structural at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of ...

2015-12-01T03:42:57Z

New page: '''Unreleased structure''' The entry 5fnx is ON HOLD Authors: Guerra, Y., Rudino-Pinera, E. Description: Crystal structural at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of ...

New page

'''Unreleased structure'''

The entry 5fnx is ON HOLD

Authors: Guerra, Y., Rudino-Pinera, E.

Description: Crystal structural at pH 9.0 of a potato STI-Kunitz bi-functional inhibitor of serine and aspartic proteases
[[Category: Unreleased Structures]]
[[Category: Rudino-Pinera, E]]
[[Category: Guerra, Y]]