5g59 - Revision history

OCA at 13:39, 26 July 2023

2023-07-26T13:39:25Z

←Older revision		Revision as of 13:39, 26 July 2023
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	<StructureSection load='5g59' size='340' side='right'caption='[[5g59]], [[Resolution\|resolution]] 1.61Å' scene=''>		<StructureSection load='5g59' size='340' side='right'caption='[[5g59]], [[Resolution\|resolution]] 1.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g59]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_43587 Atcc 43587~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G59 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5G59 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g59]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G59 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.613Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5g5c\|5g5c]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5g59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g59 OCA], [~~http~~://pdbe.org/5g59 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5g59 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5g59 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5g59 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g59 OCA], [https://pdbe.org/5g59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g59 RCSB], [https://www.ebi.ac.uk/pdbsum/5g59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g59 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8TZJ1_PYRFU Q8TZJ1_PYRFU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 43587]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Reverter, D~~]]	+	[[Category: Pyrococcus furiosus]]
-	[[Category: ~~Varejao, N~~]]	+	[[Category: Reverter D]]
-	[[Category: ~~Esterase]]~~	+	[[Category: Varejao N]]
-	~~[[Category: Structural protein]]~~	+
-	~~[[Category: Themophilic~~]]	+

OCA at 06:39, 13 May 2020

2020-05-13T06:39:30Z

←Older revision		Revision as of 06:39, 13 May 2020
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	==Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group P3121==		==Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group P3121==
-	<StructureSection load='5g59' size='340' side='right' caption='[[5g59]], [[Resolution\|resolution]] 1.61Å' scene=''>	+	<StructureSection load='5g59' size='340' side='right'caption='[[5g59]], [[Resolution\|resolution]] 1.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G59 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5G59 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G59 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5G59 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g5c\|5g5c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g5c\|5g5c]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5g59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g59 OCA], [http://pdbe.org/5g59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g59 RCSB], [http://www.ebi.ac.uk/pdbsum/5g59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g59 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5g59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g59 OCA], [http://pdbe.org/5g59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g59 RCSB], [http://www.ebi.ac.uk/pdbsum/5g59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g59 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 43587]]		[[Category: Atcc 43587]]
		+	[[Category: Large Structures]]
	[[Category: Reverter, D]]		[[Category: Reverter, D]]
	[[Category: Varejao, N]]		[[Category: Varejao, N]]

OCA at 08:05, 17 January 2018

2018-01-17T08:05:41Z

←Older revision		Revision as of 08:05, 17 January 2018
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g59 OCA], [http://pdbe.org/5g59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g59 RCSB], [http://www.ebi.ac.uk/pdbsum/5g59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g59 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g59 OCA], [http://pdbe.org/5g59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g59 RCSB], [http://www.ebi.ac.uk/pdbsum/5g59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g59 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lipases and esterases constitute a group of enzymes that catalyze the hydrolysis or synthesis of ester bonds. A major biotechnological interest corresponds to thermophilic esterases, due to their intrinsic stability at high temperatures. The Pf2001 esterase from Pyrococcus furiosus reaches its optimal activity between 70 degrees C and 80 degrees C. The crystal structure of the Pf2001 esterase shows two different conformations: monomer and dimer. The structures reveal important rearrangements in the "cap" subdomain between monomer and dimer, by the formation of an extensive intertwined helical interface. Moreover, the dimer interface is essential for the formation of the hydrophobic channel for substrate selectivity, as confirmed by mutagenesis and kinetic analysis. We also provide evidence for dimer formation at high temperatures, a process that correlates with its enzymatic activation. Thus, we propose a temperature-dependent activation mechanism of the Pf2001 esterase via dimerization that is necessary for the substrate channel formation in the active-site cleft.
		+
		+	Structural Mechanism for the Temperature-Dependent Activation of the Hyperthermophilic Pf2001 Esterase.,Varejao N, De-Andrade RA, Almeida RV, Anobom CD, Foguel D, Reverter D Structure. 2017 Dec 28. pii: S0969-2126(17)30403-3. doi:, 10.1016/j.str.2017.12.004. PMID:29307486<ref>PMID:29307486</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5g59" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 23:09, 15 November 2017

2017-11-15T23:09:20Z

←Older revision		Revision as of 23:09, 15 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5g59 is ~~ON HOLD until Paper Publication~~	+	==Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group P3121==
-		+	<StructureSection load='5g59' size='340' side='right' caption='[[5g59]], [[Resolution\|resolution]] 1.61Å' scene=''>
-	~~Authors~~: ~~Varejao~~, N., ~~Reverter~~, D.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5g59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G59 FirstGlance]. <br>
-	~~Description~~: ~~Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group P3121~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g5c\|5g5c]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g59 OCA], [http://pdbe.org/5g59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g59 RCSB], [http://www.ebi.ac.uk/pdbsum/5g59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g59 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Atcc 43587]]
		+	[[Category: Reverter, D]]
	[[Category: Varejao, N]]		[[Category: Varejao, N]]
-	[[Category: ~~Reverter, D~~]]	+	[[Category: Esterase]]
		+	[[Category: Structural protein]]
		+	[[Category: Themophilic]]

OCA at 06:33, 10 May 2017

2017-05-10T06:33:52Z

←Older revision		Revision as of 06:33, 10 May 2017
Line 7:		Line 7:
	Description: Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group P3121		Description: Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group P3121
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Reverter, D]]
	[[Category: Varejao, N]]		[[Category: Varejao, N]]
		+	[[Category: Reverter, D]]

OCA: Protected "5g59" [edit=sysop:move=sysop]

2016-06-01T11:42:32Z

Protected "5g59" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:42, 1 June 2016

OCA: New page: '''Unreleased structure''' The entry 5g59 is ON HOLD until Paper Publication Authors: Varejao, N., Reverter, D. Description: Structure of the Pyrococcus Furiosus Esterase Pf2001 with s...

2016-06-01T11:42:26Z

New page: '''Unreleased structure''' The entry 5g59 is ON HOLD until Paper Publication Authors: Varejao, N., Reverter, D. Description: Structure of the Pyrococcus Furiosus Esterase Pf2001 with s...

New page

'''Unreleased structure'''

The entry 5g59 is ON HOLD until Paper Publication

Authors: Varejao, N., Reverter, D.

Description: Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group P3121
[[Category: Unreleased Structures]]
[[Category: Reverter, D]]
[[Category: Varejao, N]]