5gzz - Revision history

OCA at 11:47, 2 August 2023

2023-08-02T11:47:31Z

←Older revision		Revision as of 11:47, 2 August 2023
Line 3:		Line 3:
	<StructureSection load='5gzz' size='340' side='right'caption='[[5gzz]], [[Resolution\|resolution]] 2.39Å' scene=''>		<StructureSection load='5gzz' size='340' side='right'caption='[[5gzz]], [[Resolution\|resolution]] 2.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gzz]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~] and [~~http~~://en.wikipedia.org/wiki/~~Blood_fluke Blood fluke~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GZZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5GZZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gzz]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Schistosoma_japonicum Schistosoma japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GZZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>~~, ~~<scene name='pdbligand=JAA:{(1R,2R)-3-OXO-2-~~[~~(2Z)-PENT-2-EN-1-YL]CYCLOPENTYL}ACETIC+ACID'>JAA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.386Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[~~[Related_structure~~\|~~Related:]]</b></td><td class="sblockDat">[[5eck\|5eck~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=JAA:{(1R,2R)-3-OXO-2-[(2Z)-PENT-2-EN-1-YL]CYCLOPENTYL}ACETIC+ACID'>JAA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>JAR1, FIN219, AtGH3.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~3702 ARATH]), SjGST ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6182 Blood fluke])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gzz OCA], [https://pdbe.org/5gzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gzz RCSB], [https://www.ebi.ac.uk/pdbsum/5gzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gzz ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase]~~, ~~with EC number~~ [~~http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=~~2~~.5.~~1~~.18 2.5.1.18~~] </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gzz OCA], [~~http~~://pdbe.org/5gzz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5gzz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5gzz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5gzz ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/JAR1_ARATH JAR1_ARATH]] Catalyzes the synthesis of jasmonates-amino acid conjugates by adenylation; can use Ile and, in vitro at least, Val, Leu and Phe as conjugating amino acids on jasmonic acid (JA) and 9,10-dihydro-JA substrates, and to a lower extent, on 3-oxo-2-(2Z-pentenyl)-cyclopentane-1-butyric acid (OPC-4) and 12-hydroxy-JA (12-OH-JA). Can synthesize adenosine 5-tetraphosphate in vitro. Required for the JA-mediated signaling pathway that regulates many developmental and defense mechanisms, including growth root inhibition, vegetative storage proteins (VSPs) accumulation, induced systemic resistance (ISR), response to wounding and herbivores, tolerance to ozone O(3) (probably having a role in lesion containment). Plays an important role in the accumulation of JA-Ile in response to wounding, both locally and systemically; promotes JA responding genes especially in distal part of wounded plants, via the JA-Ile-stimulated degradation of JAZ repressor proteins by the SCF(COI)E3 ubiquitin-protein ligase pathway. Involved in the apoptosis-like programmed cell death (PCD) induced by fungal toxin fumonisin B1-mediated (FB1). Required for volatile compounds (C6-aldehydes and allo-ocimene)-mediated defense activation. Involved in the non-pathogenic rhizobacterium-mediated ISR (defense priming) by P.fluorescens (strains CHAOr and WCS417r) and P.putida LSW17S against infection leaf pathogens such as P.syringae pv. tomato and H.parasitica. Required for the JA-dependent resistance to fungi such as P.irregulare, U.vignae and U.appendiculatus. Necessary to induce systemic resistance against R.solanaceraum and P.syringae pv. tomato with P.oligandrum (a non-pathogenic biocontrol agent) cell wall protein fraction (CWP). Mediates PGIP2 accumulation in response to B.cinerea infection and thus contributes to resistance against this pathogen. Modulates the UV-B alteration of leaves attractiveness to diamondback moths P.xylostella leading to insect oviposition. Involved in the regulation of far-red light influence on development. Seems necessary for the salicylic acid (SA)-mediated, NPR1-independent resistance pathway. May contribute to the chitin-elicited pathway. Contributes to the sensitivity toward F.graminearum.<ref>PMID:10921909</ref> <ref>PMID:11006337</ref> <ref>PMID:11041879</ref> <ref>PMID:11041881</ref> <ref>PMID:11090217</ref> <ref>PMID:11607311</ref> <ref>PMID:12084835</ref> <ref>PMID:12236603</ref> <ref>PMID:12376653</ref> <ref>PMID:12509524</ref> <ref>PMID:12744510</ref> <ref>PMID:12848825</ref> <ref>PMID:14558686</ref> <ref>PMID:15258265</ref> <ref>PMID:15879447</ref> <ref>PMID:16639567</ref> <ref>PMID:17220357</ref> <ref>PMID:17291501</ref> <ref>PMID:17601164</ref> <ref>PMID:18247047</ref> <ref>PMID:19251652</ref> <ref>PMID:19304739</ref> <ref>PMID:19473329</ref> <ref>PMID:20521949</ref> <ref>PMID:9724702</ref> <ref>PMID:9807813</ref> [[http://www.uniprot.org/uniprot/GST26_SCHJA GST26_SCHJA]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.	+	[https://www.uniprot.org/uniprot/JAR1_ARATH JAR1_ARATH] Catalyzes the synthesis of jasmonates-amino acid conjugates by adenylation; can use Ile and, in vitro at least, Val, Leu and Phe as conjugating amino acids on jasmonic acid (JA) and 9,10-dihydro-JA substrates, and to a lower extent, on 3-oxo-2-(2Z-pentenyl)-cyclopentane-1-butyric acid (OPC-4) and 12-hydroxy-JA (12-OH-JA). Can synthesize adenosine 5-tetraphosphate in vitro. Required for the JA-mediated signaling pathway that regulates many developmental and defense mechanisms, including growth root inhibition, vegetative storage proteins (VSPs) accumulation, induced systemic resistance (ISR), response to wounding and herbivores, tolerance to ozone O(3) (probably having a role in lesion containment). Plays an important role in the accumulation of JA-Ile in response to wounding, both locally and systemically; promotes JA responding genes especially in distal part of wounded plants, via the JA-Ile-stimulated degradation of JAZ repressor proteins by the SCF(COI)E3 ubiquitin-protein ligase pathway. Involved in the apoptosis-like programmed cell death (PCD) induced by fungal toxin fumonisin B1-mediated (FB1). Required for volatile compounds (C6-aldehydes and allo-ocimene)-mediated defense activation. Involved in the non-pathogenic rhizobacterium-mediated ISR (defense priming) by P.fluorescens (strains CHAOr and WCS417r) and P.putida LSW17S against infection leaf pathogens such as P.syringae pv. tomato and H.parasitica. Required for the JA-dependent resistance to fungi such as P.irregulare, U.vignae and U.appendiculatus. Necessary to induce systemic resistance against R.solanaceraum and P.syringae pv. tomato with P.oligandrum (a non-pathogenic biocontrol agent) cell wall protein fraction (CWP). Mediates PGIP2 accumulation in response to B.cinerea infection and thus contributes to resistance against this pathogen. Modulates the UV-B alteration of leaves attractiveness to diamondback moths P.xylostella leading to insect oviposition. Involved in the regulation of far-red light influence on development. Seems necessary for the salicylic acid (SA)-mediated, NPR1-independent resistance pathway. May contribute to the chitin-elicited pathway. Contributes to the sensitivity toward F.graminearum.<ref>PMID:10921909</ref> <ref>PMID:11006337</ref> <ref>PMID:11041879</ref> <ref>PMID:11041881</ref> <ref>PMID:11090217</ref> <ref>PMID:11607311</ref> <ref>PMID:12084835</ref> <ref>PMID:12236603</ref> <ref>PMID:12376653</ref> <ref>PMID:12509524</ref> <ref>PMID:12744510</ref> <ref>PMID:12848825</ref> <ref>PMID:14558686</ref> <ref>PMID:15258265</ref> <ref>PMID:15879447</ref> <ref>PMID:16639567</ref> <ref>PMID:17220357</ref> <ref>PMID:17291501</ref> <ref>PMID:17601164</ref> <ref>PMID:18247047</ref> <ref>PMID:19251652</ref> <ref>PMID:19304739</ref> <ref>PMID:19473329</ref> <ref>PMID:20521949</ref> <ref>PMID:9724702</ref> <ref>PMID:9807813</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath]]~~	+	[[Category: Arabidopsis thaliana]]
-	~~[[Category: Blood fluke]]~~	+
-	~~[[Category: Glutathione transferase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chen, C Y~~]]	+	[[Category: Schistosoma japonicum]]
-	[[Category: ~~Cheng, Y S~~]]	+	[[Category: Chen CY]]
-	[[Category: ~~Glutathione s-transferase]]~~	+	[[Category: Cheng YS]]
-	~~[[Category: Jasmonate-amido synthetase]]~~	+
-	~~[[Category: Ligase-transferase complex~~]]	+

OCA at 06:48, 20 May 2020

2020-05-20T06:48:01Z

←Older revision		Revision as of 06:48, 20 May 2020
Line 1:		Line 1:

	==Crystal Structure of FIN219-SjGST complex with JA==		==Crystal Structure of FIN219-SjGST complex with JA==
-	<StructureSection load='5gzz' size='340' side='right' caption='[[5gzz]], [[Resolution\|resolution]] 2.39Å' scene=''>	+	<StructureSection load='5gzz' size='340' side='right'caption='[[5gzz]], [[Resolution\|resolution]] 2.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gzz]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GZZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GZZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gzz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath] and [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GZZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GZZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=JAA:{(1R,2R)-3-OXO-2-[(2Z)-PENT-2-EN-1-YL]CYCLOPENTYL}ACETIC+ACID'>JAA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=JAA:{(1R,2R)-3-OXO-2-[(2Z)-PENT-2-EN-1-YL]CYCLOPENTYL}ACETIC+ACID'>JAA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eck\|5eck]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eck\|5eck]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">JAR1, FIN219, AtGH3.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), SjGST ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6182 Blood fluke])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gzz OCA], [http://pdbe.org/5gzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gzz RCSB], [http://www.ebi.ac.uk/pdbsum/5gzz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gzz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gzz OCA], [http://pdbe.org/5gzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gzz RCSB], [http://www.ebi.ac.uk/pdbsum/5gzz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gzz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 5gzz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5gzz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
		+	[[Category: Blood fluke]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
		+	[[Category: Large Structures]]
	[[Category: Chen, C Y]]		[[Category: Chen, C Y]]
	[[Category: Cheng, Y S]]		[[Category: Cheng, Y S]]

OCA at 07:04, 9 March 2017

2017-03-09T07:04:08Z

←Older revision		Revision as of 07:04, 9 March 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5gzz is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal Structure of FIN219-SjGST complex with JA==
		+	<StructureSection load='5gzz' size='340' side='right' caption='[[5gzz]], [[Resolution\|resolution]] 2.39Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5gzz]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GZZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=JAA:{(1R,2R)-3-OXO-2-[(2Z)-PENT-2-EN-1-YL]CYCLOPENTYL}ACETIC+ACID'>JAA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5eck\|5eck]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gzz OCA], [http://pdbe.org/5gzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gzz RCSB], [http://www.ebi.ac.uk/pdbsum/5gzz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gzz ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/JAR1_ARATH JAR1_ARATH]] Catalyzes the synthesis of jasmonates-amino acid conjugates by adenylation; can use Ile and, in vitro at least, Val, Leu and Phe as conjugating amino acids on jasmonic acid (JA) and 9,10-dihydro-JA substrates, and to a lower extent, on 3-oxo-2-(2Z-pentenyl)-cyclopentane-1-butyric acid (OPC-4) and 12-hydroxy-JA (12-OH-JA). Can synthesize adenosine 5-tetraphosphate in vitro. Required for the JA-mediated signaling pathway that regulates many developmental and defense mechanisms, including growth root inhibition, vegetative storage proteins (VSPs) accumulation, induced systemic resistance (ISR), response to wounding and herbivores, tolerance to ozone O(3) (probably having a role in lesion containment). Plays an important role in the accumulation of JA-Ile in response to wounding, both locally and systemically; promotes JA responding genes especially in distal part of wounded plants, via the JA-Ile-stimulated degradation of JAZ repressor proteins by the SCF(COI)E3 ubiquitin-protein ligase pathway. Involved in the apoptosis-like programmed cell death (PCD) induced by fungal toxin fumonisin B1-mediated (FB1). Required for volatile compounds (C6-aldehydes and allo-ocimene)-mediated defense activation. Involved in the non-pathogenic rhizobacterium-mediated ISR (defense priming) by P.fluorescens (strains CHAOr and WCS417r) and P.putida LSW17S against infection leaf pathogens such as P.syringae pv. tomato and H.parasitica. Required for the JA-dependent resistance to fungi such as P.irregulare, U.vignae and U.appendiculatus. Necessary to induce systemic resistance against R.solanaceraum and P.syringae pv. tomato with P.oligandrum (a non-pathogenic biocontrol agent) cell wall protein fraction (CWP). Mediates PGIP2 accumulation in response to B.cinerea infection and thus contributes to resistance against this pathogen. Modulates the UV-B alteration of leaves attractiveness to diamondback moths P.xylostella leading to insect oviposition. Involved in the regulation of far-red light influence on development. Seems necessary for the salicylic acid (SA)-mediated, NPR1-independent resistance pathway. May contribute to the chitin-elicited pathway. Contributes to the sensitivity toward F.graminearum.<ref>PMID:10921909</ref> <ref>PMID:11006337</ref> <ref>PMID:11041879</ref> <ref>PMID:11041881</ref> <ref>PMID:11090217</ref> <ref>PMID:11607311</ref> <ref>PMID:12084835</ref> <ref>PMID:12236603</ref> <ref>PMID:12376653</ref> <ref>PMID:12509524</ref> <ref>PMID:12744510</ref> <ref>PMID:12848825</ref> <ref>PMID:14558686</ref> <ref>PMID:15258265</ref> <ref>PMID:15879447</ref> <ref>PMID:16639567</ref> <ref>PMID:17220357</ref> <ref>PMID:17291501</ref> <ref>PMID:17601164</ref> <ref>PMID:18247047</ref> <ref>PMID:19251652</ref> <ref>PMID:19304739</ref> <ref>PMID:19473329</ref> <ref>PMID:20521949</ref> <ref>PMID:9724702</ref> <ref>PMID:9807813</ref> [[http://www.uniprot.org/uniprot/GST26_SCHJA GST26_SCHJA]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Far-red (FR) light-coupled jasmonate (JA) signaling is necessary for plant defense and development. FR insensitive 219 (FIN219) is a member of the Gretchen Hagen 3 (GH3) family of proteins in Arabidopsis and belongs to the adenylate-forming family of enzymes. It directly controls biosynthesis of jasmonoyl-isoleucine in JA-mediated defense responses and interacts with FIN219-interacting protein 1 (FIP1) under FR light conditions. FIN219 and FIP1 are involved in FR light signaling and are regulators of the interplay between light and JA signaling. However, how their interactions affect plant physiological functions remains unclear. Here, we demonstrate the crystal structures of FIN219-FIP1 while binding with substrates at atomic resolution. Our results show an unexpected FIN219 conformation and demonstrate various differences between this protein and other members of the GH3 family. We show that the rotated C-terminal domain of FIN219 alters ATP binding and the core structure of the active site. We further demonstrate that this unique FIN219-FIP1 structure is crucial for increasing FIN219 activity and determines the priority of substrate binding. We suggest that the increased FIN219 activity resulting from the complex form, a conformation for domain switching, allows FIN219 to switch to its high-affinity mode and thereby enhances JA signaling under continuous FR light conditions.

-	~~Authors:~~ Chen, C.Y., ~~Cheng, Y~~.S.	+	Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation.,Chen CY, Ho SS, Kuo TY, Hsieh HL, Cheng YS Proc Natl Acad Sci U S A. 2017 Feb 21. pii: 201609980. doi:, 10.1073/pnas.1609980114. PMID:28223489<ref>PMID:28223489</ref>

-	~~Description: Crystal Structure~~ of ~~FIN219~~-~~SjGST complex with JA~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Cheng~~, Y.S]]	+	<div class="pdbe-citations 5gzz" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Chen~~, C.Y]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Glutathione transferase]]
		+	[[Category: Chen, C Y]]
		+	[[Category: Cheng, Y S]]
		+	[[Category: Glutathione s-transferase]]
		+	[[Category: Jasmonate-amido synthetase]]
		+	[[Category: Ligase-transferase complex]]

OCA at 12:48, 2 November 2016

2016-11-02T12:48:03Z

←Older revision		Revision as of 12:48, 2 November 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5gzz is ON HOLD	+	The entry 5gzz is ON HOLD until Paper Publication

	Authors: Chen, C.Y., Cheng, Y.S.		Authors: Chen, C.Y., Cheng, Y.S.

OCA: Protected "5gzz" [edit=sysop:move=sysop]

2016-10-19T11:58:47Z

Protected "5gzz" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:58, 19 October 2016

OCA: New page: '''Unreleased structure''' The entry 5gzz is ON HOLD Authors: Chen, C.Y., Cheng, Y.S. Description: Crystal Structure of FIN219-SjGST complex with JA Category: Unreleased Structures...

2016-10-19T11:58:46Z

New page: '''Unreleased structure''' The entry 5gzz is ON HOLD Authors: Chen, C.Y., Cheng, Y.S. Description: Crystal Structure of FIN219-SjGST complex with JA Category: Unreleased Structures...

New page

'''Unreleased structure'''

The entry 5gzz is ON HOLD

Authors: Chen, C.Y., Cheng, Y.S.

Description: Crystal Structure of FIN219-SjGST complex with JA
[[Category: Unreleased Structures]]
[[Category: Cheng, Y.S]]
[[Category: Chen, C.Y]]