5h79 - Revision history

OCA at 07:26, 9 August 2023

2023-08-09T07:26:06Z

←Older revision		Revision as of 07:26, 9 August 2023
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	==Crystal structure of a repeat protein with three Protein A repeat module==		==Crystal structure of a repeat protein with three Protein A repeat module==
-	<StructureSection load='5h79' size='340' side='right' caption='[[5h79]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='5h79' size='340' side='right'caption='[[5h79]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5h79]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H79 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5H79 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5h79]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H79 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H79 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5h75\|5h75]], [[5h76\|5h76]], [[5h77\|5h77]], [[5h78\|5h78]], [[5h7a\|5h7a]], [[5h7b\|5h7b]], [[5h7c\|5h7c]], [[5h7d\|5h7d]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">spa ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h79 OCA], [https://pdbe.org/5h79 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h79 RCSB], [https://www.ebi.ac.uk/pdbsum/5h79 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h79 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5h79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h79 OCA], [~~http~~://pdbe.org/5h79 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5h79 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5h79 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5h79 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPA_STAAU SPA_STAAU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Kim, J H~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kwon, N Y~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Lee, H~~]]	+	[[Category: Kim JH]]
-	[[Category: ~~Lee, J H~~]]	+	[[Category: Kwon NY]]
-	[[Category: Lee~~, J O~~]]	+	[[Category: Lee H]]
-	[[Category: ~~Youn, S J~~]]	+	[[Category: Lee JH]]
-	[[Category: ~~Immune system~~]]	+	[[Category: Lee JO]]
-	[[Category: ~~Synthetic protein~~]]	+	[[Category: Youn SJ]]

OCA at 10:48, 6 November 2017

2017-11-06T10:48:04Z

←Older revision		Revision as of 10:48, 6 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5h79 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of a repeat protein with three Protein A repeat module==
		+	<StructureSection load='5h79' size='340' side='right' caption='[[5h79]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5h79]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H79 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H79 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h75\|5h75]], [[5h76\|5h76]], [[5h77\|5h77]], [[5h78\|5h78]], [[5h7a\|5h7a]], [[5h7b\|5h7b]], [[5h7c\|5h7c]], [[5h7d\|5h7d]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h79 OCA], [http://pdbe.org/5h79 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h79 RCSB], [http://www.ebi.ac.uk/pdbsum/5h79 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h79 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Generating artificial protein assemblies with complex shapes requires a method for connecting protein components with stable and predictable structures. Currently available methods for creating rigid protein assemblies rely on either complicated calculations or extensive trial and error. We describe a simple and efficient method for connecting two proteins via a fused alpha helix that is formed by joining two preexisting helices into a single extended helix. Because the end-to-end ligation of helices does not guarantee the formation of a continuous helix, we superimposed 1-2 turns of pairs of connecting helices by using a molecular graphics program. Then, we chose amino acids from the two natural sequences that would stabilize the connecting helix. This "shared helix method" is highly efficient. All the designed proteins that could be produced in Escherichia coli were readily crystallized and had the expected fusion structures. To prove the usefulness of this method, we produced two novel repeat proteins by assembling several copies of natural or artificial proteins with alpha helices at both termini. Their crystal structures demonstrated the successful assembly of the repeating units with the intended curved shapes. We propose that this method could dramatically expand the available repertoire of natural repeat proteins.

-	~~Authors~~:	+	Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.,Youn SJ, Kwon NY, Lee JH, Kim JH, Choi J, Lee H, Lee JO Sci Rep. 2017 Jun 1;7(1):2595. doi: 10.1038/s41598-017-02803-z. PMID:28572639<ref>PMID:28572639</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5h79" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Kim, J H]]
		+	[[Category: Kwon, N Y]]
		+	[[Category: Lee, H]]
		+	[[Category: Lee, J H]]
		+	[[Category: Lee, J O]]
		+	[[Category: Youn, S J]]
		+	[[Category: Immune system]]
		+	[[Category: Synthetic protein]]

OCA: Protected "5h79" [edit=sysop:move=sysop]

2016-12-09T13:50:00Z

Protected "5h79" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:50, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5h79 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2016-12-09T13:49:58Z

New page: '''Unreleased structure''' The entry 5h79 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5h79 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]