5hbz - Revision history

OCA at 07:30, 9 August 2023

2023-08-09T07:30:14Z

←Older revision		Revision as of 07:30, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='5hbz' size='340' side='right'caption='[[5hbz]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='5hbz' size='340' side='right'caption='[[5hbz]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hbz]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eav Eav~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HBZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5HBZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hbz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Equine_arteritis_virus_Bucyrus Equine arteritis virus Bucyrus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HBZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HBZ FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5hc1\|5hc1]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">rep, 1a-1b ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=299386 EAV])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hbz OCA], [https://pdbe.org/5hbz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hbz RCSB], [https://www.ebi.ac.uk/pdbsum/5hbz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hbz ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5hbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hbz OCA], [~~http~~://pdbe.org/5hbz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hbz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hbz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hbz ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RPOA_EAVBU RPOA_EAVBU]] The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.<ref>PMID:18078692</ref> Nsp1 is essential for viral subgenomic mRNA synthesis.<ref>PMID:18078692</ref> Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity.<ref>PMID:18078692</ref> The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.<ref>PMID:18078692</ref> The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.<ref>PMID:18078692</ref>	+	[https://www.uniprot.org/uniprot/RPOA_EAVBU RPOA_EAVBU] The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.<ref>PMID:18078692</ref> Nsp1 is essential for viral subgenomic mRNA synthesis.<ref>PMID:18078692</ref> Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity.<ref>PMID:18078692</ref> The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.<ref>PMID:18078692</ref> The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.<ref>PMID:18078692</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Eav~~]]	+	[[Category: Equine arteritis virus Bucyrus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen~~, Z Z~~]]	+	[[Category: Chen ZZ]]
-	[[Category: Zhang~~, M F]]~~	+	[[Category: Zhang MF]]
-	~~[[Category: Endoribonuclease]]~~	+
-	~~[[Category: Equine arteritis virus]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Nf-kappab]]~~	+
-	~~[[Category: Nidovirus]]~~	+
-	~~[[Category: Nonstructural protein 11]]~~	+
-	~~[[Category: Nsp11~~]]	+

OCA at 06:58, 20 May 2020

2020-05-20T06:58:55Z

←Older revision		Revision as of 06:58, 20 May 2020
Line 1:		Line 1:

	==Structure of EAV NSP11 K170A mutant at 3.10A==		==Structure of EAV NSP11 K170A mutant at 3.10A==
-	<StructureSection load='5hbz' size='340' side='right' caption='[[5hbz]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<StructureSection load='5hbz' size='340' side='right'caption='[[5hbz]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hbz]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HBZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HBZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hbz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Eav Eav]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HBZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HBZ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hc1\|5hc1]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hc1\|5hc1]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hbz OCA], [http://pdbe.org/5hbz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hbz RCSB], [http://www.ebi.ac.uk/pdbsum/5hbz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hbz ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rep, 1a-1b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=299386 EAV])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hbz OCA], [http://pdbe.org/5hbz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hbz RCSB], [http://www.ebi.ac.uk/pdbsum/5hbz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hbz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Eav]]
		+	[[Category: Large Structures]]
	[[Category: Chen, Z Z]]		[[Category: Chen, Z Z]]
	[[Category: Zhang, M F]]		[[Category: Zhang, M F]]

OCA at 03:37, 10 December 2016

2016-12-10T03:37:17Z

←Older revision		Revision as of 03:37, 10 December 2016
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/RPOA_EAVBU RPOA_EAVBU]] The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.<ref>PMID:18078692</ref> Nsp1 is essential for viral subgenomic mRNA synthesis.<ref>PMID:18078692</ref> Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity.<ref>PMID:18078692</ref> The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.<ref>PMID:18078692</ref> The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.<ref>PMID:18078692</ref>		[[http://www.uniprot.org/uniprot/RPOA_EAVBU RPOA_EAVBU]] The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.<ref>PMID:18078692</ref> Nsp1 is essential for viral subgenomic mRNA synthesis.<ref>PMID:18078692</ref> Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity.<ref>PMID:18078692</ref> The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.<ref>PMID:18078692</ref> The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.<ref>PMID:18078692</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Endoribonuclease (NendoU) is unique and conserved as a major genetic marker in nidoviruses that infect vertebrate hosts. Arterivirus non-structural protein (nsp) 11 was shown to have NendoU activity and play essential roles in the viral life cycle. Here, we report three crystal structures of porcine reproductive and respiratory syndrome virus (PRRSV) and equine arteritis virus (EAV) nsp11 mutants. The structures of arterivirus nsp11 contain two conserved compact domains: N-terminal domain (NTD) and C-terminal domain (CTD). The structures of PRRSV and EAV endoribonucleases are similar and conserved in the arterivirus, but they are greatly different from that of severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoV) representing important human pathogens in the Nidovirales order. The catalytic center of NendoU activity is located in the CTD, where a positively charged groove is next to the key catalytic residues conserved in nidoviruses. Although the NTD is nearly identical, the catalytic region of the arterivirus nsp11 family proteins is remarkably flexible, and the oligomerization may be concentration-dependent. In summary, our structures provide new insight into this key multifunctional NendoU family of proteins and lay a foundation for better understanding of the molecular mechanism and antiviral drug development. IMPORTANCE: Porcine reproductive and respiratory syndrome virus (PRRSV) and equine arteritis virus are two major members of the arterivirus family. PRRSV, a leading swine pathogen, causes reproductive failure in breeding stock and respiratory tract illness in young pigs. Due to the lack of a suitable vaccine or effective drug treatment and the quick spread of these viruses, infected animals either die quickly or must be culled. PRRSV costs the swine industry around $644 million annually in the USA and almost 1.5b[euro] in Europe every year. To find a way to combat these viruses, we focused on the essential viral non-structural protein (nsp) 11. Nsp11 is associated with multiple functions such as RNA-processing and suppressing the infected host innate immunity system. The three solved structures in this study provide new insight into the molecular mechanisms of this crucial protein family and will benefit the development of new treatments against these deadly viruses.
		+
		+	Structural biology of the arterivirus nsp11 endoribonucleases.,Zhang M, Li X, Deng Z, Chen Z, Liu Y, Gao Y, Wu W, Chen Z J Virol. 2016 Oct 19. pii: JVI.01309-16. PMID:27795409<ref>PMID:27795409</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5hbz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:39, 19 October 2016

2016-10-19T17:39:52Z

←Older revision		Revision as of 17:39, 19 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5hbz is ~~ON HOLD until Paper Publication~~	+	==Structure of EAV NSP11 K170A mutant at 3.10A==
-		+	<StructureSection load='5hbz' size='340' side='right' caption='[[5hbz]], [[Resolution\|resolution]] 3.10Å' scene=''>
-	~~Authors~~: ~~Zhang~~, M.F., ~~Chen~~, Z.Z.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5hbz]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HBZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HBZ FirstGlance]. <br>
-	~~Description~~: ~~Structure~~ of ~~EAV NSP11 at~~ 3.~~10A~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hc1\|5hc1]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hbz OCA], [http://pdbe.org/5hbz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hbz RCSB], [http://www.ebi.ac.uk/pdbsum/5hbz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hbz ProSAT]</span></td></tr>
-	[[Category: Zhang, M.F]]	+	</table>
-	[[Category: ~~Chen, Z.Z~~]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RPOA_EAVBU RPOA_EAVBU]] The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.<ref>PMID:18078692</ref> Nsp1 is essential for viral subgenomic mRNA synthesis.<ref>PMID:18078692</ref> Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity.<ref>PMID:18078692</ref> The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.<ref>PMID:18078692</ref> The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.<ref>PMID:18078692</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, Z Z]]
		+	[[Category: Zhang, M F]]
		+	[[Category: Endoribonuclease]]
		+	[[Category: Equine arteritis virus]]
		+	[[Category: Hydrolase]]
		+	[[Category: Nf-kappab]]
		+	[[Category: Nidovirus]]
		+	[[Category: Nonstructural protein 11]]
		+	[[Category: Nsp11]]

OCA at 15:13, 20 January 2016

2016-01-20T15:13:55Z

←Older revision		Revision as of 15:13, 20 January 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hbz is ON HOLD	+	The entry 5hbz is ON HOLD until Paper Publication

	Authors: Zhang, M.F., Chen, Z.Z.		Authors: Zhang, M.F., Chen, Z.Z.

OCA: Protected "5hbz" [edit=sysop:move=sysop]

2016-01-13T18:47:18Z

Protected "5hbz" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:47, 13 January 2016

OCA: New page: '''Unreleased structure''' The entry 5hbz is ON HOLD Authors: Zhang, M.F., Chen, Z.Z. Description: Structure of EAV NSP11 at 3.10A Category: Unreleased Structures [[Category: Zhang...

2016-01-13T18:47:16Z

New page: '''Unreleased structure''' The entry 5hbz is ON HOLD Authors: Zhang, M.F., Chen, Z.Z. Description: Structure of EAV NSP11 at 3.10A Category: Unreleased Structures [[Category: Zhang...

New page

'''Unreleased structure'''

The entry 5hbz is ON HOLD

Authors: Zhang, M.F., Chen, Z.Z.

Description: Structure of EAV NSP11 at 3.10A
[[Category: Unreleased Structures]]
[[Category: Zhang, M.F]]
[[Category: Chen, Z.Z]]