5hgj - Revision history

OCA at 12:32, 6 March 2024

2024-03-06T12:32:19Z

←Older revision		Revision as of 12:32, 6 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ITA1_HUMAN ITA1_HUMAN] Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen.		[https://www.uniprot.org/uniprot/ITA1_HUMAN ITA1_HUMAN] Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Integrins are transmembrane cell-extracellular matrix adhesion receptors that impact many cellular functions. A subgroup of integrins contain an inserted (I) domain within the alpha-subunits (alphaI) that mediate ligand recognition where function is contingent on binding a divalent cation at the metal ion dependent adhesion site (MIDAS). Ca(2+) is reported to promote alpha1I but inhibit alpha2I ligand binding. We co-crystallized individual I-domains with MIDAS-bound Ca(2+) and report structures at 1.4 and 2.15 A resolution, respectively. Both structures are in the "closed" ligand binding conformation where Ca(2+) induces minimal global structural changes. Comparisons with Mg(2+)-bound structures reveal Mg(2+) and Ca(2+) bind alpha1I in a manner sufficient to promote ligand binding. In contrast, Ca(2+) is displaced in the alpha2I domain MIDAS by 1.4 A relative to Mg(2+) and unable to directly coordinate all MIDAS residues. We identified an E152-R192 salt bridge hypothesized to limit the flexibility of the alpha2I MIDAS, thus, reducing Ca(2+) binding. A alpha2I E152A construct resulted in a 10,000-fold increase in Mg(2+) and Ca(2+) binding affinity while increasing binding to collagen ligands 20%. These data indicate the E152-R192 salt bridge is a key distinction in the molecular mechanism of differential ion binding of these two I domains.
-
-	Salt-bridge modulates differential calcium-mediated ligand binding to integrin alpha1- and alpha2-I domains.,Brown KL, Banerjee S, Feigley A, Abe H, Blackwell TS, Pozzi A, Hudson BG, Zent R Sci Rep. 2018 Feb 13;8(1):2916. doi: 10.1038/s41598-018-21231-1. PMID:29440721<ref>PMID:29440721</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5hgj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Integrin 3D structures\|Integrin 3D structures]]		*[[Integrin 3D structures\|Integrin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:35, 9 August 2023

2023-08-09T07:35:44Z

←Older revision		Revision as of 07:35, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='5hgj' size='340' side='right'caption='[[5hgj]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='5hgj' size='340' side='right'caption='[[5hgj]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hgj]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HGJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HGJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hgj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HGJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.399Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ITGA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hgj OCA], [~~http~~://pdbe.org/5hgj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hgj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hgj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hgj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hgj OCA], [https://pdbe.org/5hgj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hgj RCSB], [https://www.ebi.ac.uk/pdbsum/5hgj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hgj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ITA1_HUMAN ITA1_HUMAN]] Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen.	+	[https://www.uniprot.org/uniprot/ITA1_HUMAN ITA1_HUMAN] Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abe, H]]	+	[[Category: Abe H]]
-	[[Category: Banerjee, S]]	+	[[Category: Banerjee S]]
-	[[Category: Blackwell, T]]	+	[[Category: Blackwell T]]
-	[[Category: Brown~~, K L~~]]	+	[[Category: Brown KL]]
-	[[Category: Feigley, A]]	+	[[Category: Feigley A]]
-	[[Category: Hudson~~, B H~~]]	+	[[Category: Hudson BH]]
-	[[Category: Pozzi, A]]	+	[[Category: Pozzi A]]
-	[[Category: Zent, R]]	+	[[Category: Zent R]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: I-domain]]~~	+
-	~~[[Category: Integrin]]~~	+
-	~~[[Category: Rossman]]~~	+
-	~~[[Category: Signaling~~]]	+

OCA at 09:05, 4 December 2019

2019-12-04T09:05:31Z

←Older revision		Revision as of 09:05, 4 December 2019
Line 1:		Line 1:

	==Structure of integrin alpha1beta1 and alpha2beta1 I-domains explain differential calcium-mediated ligand recognition==		==Structure of integrin alpha1beta1 and alpha2beta1 I-domains explain differential calcium-mediated ligand recognition==
-	<StructureSection load='5hgj' size='340' side='right' caption='[[5hgj]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='5hgj' size='340' side='right'caption='[[5hgj]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5hgj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HGJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HGJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[5hgj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HGJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HGJ FirstGlance]. <br>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5hgj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5hgj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Integrin 3D structures\|Integrin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Abe, H]]		[[Category: Abe, H]]
	[[Category: Banerjee, S]]		[[Category: Banerjee, S]]

OCA at 07:05, 28 February 2018

2018-02-28T07:05:32Z

←Older revision		Revision as of 07:05, 28 February 2018
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ITA1_HUMAN ITA1_HUMAN]] Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen.		[[http://www.uniprot.org/uniprot/ITA1_HUMAN ITA1_HUMAN]] Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Integrins are transmembrane cell-extracellular matrix adhesion receptors that impact many cellular functions. A subgroup of integrins contain an inserted (I) domain within the alpha-subunits (alphaI) that mediate ligand recognition where function is contingent on binding a divalent cation at the metal ion dependent adhesion site (MIDAS). Ca(2+) is reported to promote alpha1I but inhibit alpha2I ligand binding. We co-crystallized individual I-domains with MIDAS-bound Ca(2+) and report structures at 1.4 and 2.15 A resolution, respectively. Both structures are in the "closed" ligand binding conformation where Ca(2+) induces minimal global structural changes. Comparisons with Mg(2+)-bound structures reveal Mg(2+) and Ca(2+) bind alpha1I in a manner sufficient to promote ligand binding. In contrast, Ca(2+) is displaced in the alpha2I domain MIDAS by 1.4 A relative to Mg(2+) and unable to directly coordinate all MIDAS residues. We identified an E152-R192 salt bridge hypothesized to limit the flexibility of the alpha2I MIDAS, thus, reducing Ca(2+) binding. A alpha2I E152A construct resulted in a 10,000-fold increase in Mg(2+) and Ca(2+) binding affinity while increasing binding to collagen ligands 20%. These data indicate the E152-R192 salt bridge is a key distinction in the molecular mechanism of differential ion binding of these two I domains.
		+
		+	Salt-bridge modulates differential calcium-mediated ligand binding to integrin alpha1- and alpha2-I domains.,Brown KL, Banerjee S, Feigley A, Abe H, Blackwell TS, Pozzi A, Hudson BG, Zent R Sci Rep. 2018 Feb 13;8(1):2916. doi: 10.1038/s41598-018-21231-1. PMID:29440721<ref>PMID:29440721</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5hgj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:22, 15 November 2017

2017-11-15T17:22:33Z

←Older revision		Revision as of 17:22, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5hgj' size='340' side='right' caption='[[5hgj]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='5hgj' size='340' side='right' caption='[[5hgj]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hgj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HGJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HGJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hgj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HGJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HGJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ITGA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hgj OCA], [http://pdbe.org/5hgj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hgj RCSB], [http://www.ebi.ac.uk/pdbsum/5hgj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hgj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hgj OCA], [http://pdbe.org/5hgj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hgj RCSB], [http://www.ebi.ac.uk/pdbsum/5hgj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hgj ProSAT]</span></td></tr>
	</table>		</table>
Line 11:		Line 12:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Abe, H]]		[[Category: Abe, H]]
	[[Category: Banerjee, S]]		[[Category: Banerjee, S]]

OCA at 11:41, 12 April 2017

2017-04-12T11:41:50Z

←Older revision		Revision as of 11:41, 12 April 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5hgj is ~~ON HOLD until Paper Publication~~	+	==Structure of integrin alpha1beta1 and alpha2beta1 I-domains explain differential calcium-mediated ligand recognition==
-		+	<StructureSection load='5hgj' size='340' side='right' caption='[[5hgj]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5hgj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HGJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HGJ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hgj OCA], [http://pdbe.org/5hgj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hgj RCSB], [http://www.ebi.ac.uk/pdbsum/5hgj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hgj ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ITA1_HUMAN ITA1_HUMAN]] Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Abe, H]]
		+	[[Category: Banerjee, S]]
		+	[[Category: Blackwell, T]]
		+	[[Category: Brown, K L]]
		+	[[Category: Feigley, A]]
		+	[[Category: Hudson, B H]]
		+	[[Category: Pozzi, A]]
		+	[[Category: Zent, R]]
		+	[[Category: Cell adhesion]]
		+	[[Category: I-domain]]
		+	[[Category: Integrin]]
		+	[[Category: Rossman]]
		+	[[Category: Signaling]]

OCA at 13:36, 15 May 2016

2016-05-15T13:36:26Z

←Older revision		Revision as of 13:36, 15 May 2016
Line 3:		Line 3:
	The entry 5hgj is ON HOLD until Paper Publication		The entry 5hgj is ON HOLD until Paper Publication

-	Authors: ~~Brown, K.L., Banerjee, S., Feigley, A., Abe, H., Blackwell, T., Zent, R., Pozzi, A., Hudson, B.H.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Blackwell, T]]
-	[[Category: Abe, H]]
-	[[Category: Hudson, B.H]]
-	[[Category: Feigley, A]]
-	[[Category: Banerjee, S]]
-	[[Category: Pozzi, A]]
-	[[Category: Brown, K.L]]
-	[[Category: Zent, R]]

OCA at 13:44, 3 February 2016

2016-02-03T13:44:41Z

←Older revision		Revision as of 13:44, 3 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hgj is ON HOLD	+	The entry 5hgj is ON HOLD until Paper Publication

-	Authors: Brown, K.L, Banerjee, S., Feigley, A., Abe, H., Blackwell, T., Zent, R., Pozzi, A., Hudson, B.H.	+	Authors: Brown, K.L., Banerjee, S., Feigley, A., Abe, H., Blackwell, T., Zent, R., Pozzi, A., Hudson, B.H.

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Blackwell, T]]		[[Category: Blackwell, T]]
-	[[Category: Brown, K.L, Banerjee, S]]
	[[Category: Abe, H]]		[[Category: Abe, H]]
	[[Category: Hudson, B.H]]		[[Category: Hudson, B.H]]
	[[Category: Feigley, A]]		[[Category: Feigley, A]]
		+	[[Category: Banerjee, S]]
	[[Category: Pozzi, A]]		[[Category: Pozzi, A]]
		+	[[Category: Brown, K.L]]
	[[Category: Zent, R]]		[[Category: Zent, R]]

OCA: Protected "5hgj" [edit=sysop:move=sysop]

2016-01-20T15:23:22Z

Protected "5hgj" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:23, 20 January 2016

OCA: New page: '''Unreleased structure''' The entry 5hgj is ON HOLD Authors: Brown, K.L, Banerjee, S., Feigley, A., Abe, H., Blackwell, T., Zent, R., Pozzi, A., Hudson, B.H. Description: [[Category:...

2016-01-20T15:23:21Z

New page: '''Unreleased structure''' The entry 5hgj is ON HOLD Authors: Brown, K.L, Banerjee, S., Feigley, A., Abe, H., Blackwell, T., Zent, R., Pozzi, A., Hudson, B.H. Description: [[Category:...

New page

'''Unreleased structure'''

The entry 5hgj is ON HOLD

Authors: Brown, K.L, Banerjee, S., Feigley, A., Abe, H., Blackwell, T., Zent, R., Pozzi, A., Hudson, B.H.

Description:
[[Category: Unreleased Structures]]
[[Category: Blackwell, T]]
[[Category: Brown, K.L, Banerjee, S]]
[[Category: Abe, H]]
[[Category: Hudson, B.H]]
[[Category: Feigley, A]]
[[Category: Pozzi, A]]
[[Category: Zent, R]]