5hh1 - Revision history

OCA at 07:36, 9 August 2023

2023-08-09T07:36:19Z

←Older revision		Revision as of 07:36, 9 August 2023
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	==Crystal structure of human Naa60 mutant - F34A in complex with CoA==		==Crystal structure of human Naa60 mutant - F34A in complex with CoA==
-	<StructureSection load='5hh1' size='340' side='right' caption='[[5hh1]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5hh1' size='340' side='right'caption='[[5hh1]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hh1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HH1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HH1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hh1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HH1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.803Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hh0\|5hh0]]~~, [[~~5hgz~~\|~~5hgz~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~NAA60, HAT4, NAT15, UNQ2771/PRO7155 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hh1 OCA], [https://pdbe.org/5hh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hh1 RCSB], [https://www.ebi.ac.uk/pdbsum/5hh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hh1 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hh1 OCA], [~~http~~://pdbe.org/5hh1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hh1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hh1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hh1 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NAA60_HUMAN NAA60_HUMAN]] Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase.<ref>PMID:21750686</ref> <ref>PMID:21981917</ref>	+	[https://www.uniprot.org/uniprot/NAA60_HUMAN NAA60_HUMAN] Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase.<ref>PMID:21750686</ref> <ref>PMID:21981917</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Chen, J Y~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Liu, L~~]]	+	[[Category: Chen JY]]
-	[[Category: ~~Yun, C H~~]]	+	[[Category: Liu L]]
-	[[Category: ~~N-terminal acetylation]]~~	+	[[Category: Yun CH]]
-	~~[[Category: Nat]]~~	+
-	~~[[Category: Protein modification]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 12:47, 6 November 2017

2017-11-06T12:47:36Z

←Older revision		Revision as of 12:47, 6 November 2017
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	<StructureSection load='5hh1' size='340' side='right' caption='[[5hh1]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5hh1' size='340' side='right' caption='[[5hh1]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hh1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HH1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HH1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hh0\|5hh0]], [[5hgz\|5hgz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hh0\|5hh0]], [[5hgz\|5hgz]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NAA60, HAT4, NAT15, UNQ2771/PRO7155 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hh1 OCA], [http://pdbe.org/5hh1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hh1 RCSB], [http://www.ebi.ac.uk/pdbsum/5hh1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hh1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hh1 OCA], [http://pdbe.org/5hh1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hh1 RCSB], [http://www.ebi.ac.uk/pdbsum/5hh1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hh1 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Chen, J Y]]		[[Category: Chen, J Y]]
	[[Category: Liu, L]]		[[Category: Liu, L]]

OCA at 15:16, 14 September 2016

2016-09-14T15:16:27Z

←Older revision		Revision as of 15:16, 14 September 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5hh1 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of human Naa60 mutant - F34A in complex with CoA==
		+	<StructureSection load='5hh1' size='340' side='right' caption='[[5hh1]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5hh1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HH1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hh0\|5hh0]], [[5hgz\|5hgz]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hh1 OCA], [http://pdbe.org/5hh1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hh1 RCSB], [http://www.ebi.ac.uk/pdbsum/5hh1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hh1 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NAA60_HUMAN NAA60_HUMAN]] Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase.<ref>PMID:21750686</ref> <ref>PMID:21981917</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	N-terminal acetylation (Nt-acetylation), carried out by N-terminal acetyltransferases (NATs), is a conserved and primary modification of nascent peptide chains. Naa60 (also named NatF) is a recently identified NAT found only in multicellular eukaryotes. This protein was shown to locate on the Golgi apparatus and mainly catalyze the Nt-acetylation of transmembrane proteins, and it also harbors lysine N(epsilon)-acetyltransferase (KAT) activity to catalyze the acetylation of lysine epsilon-amine. Here, we report the crystal structures of human Naa60 (hNaa60) in complex with Acetyl-Coenzyme A (Ac-CoA) or Coenzyme A (CoA). The hNaa60 protein contains an amphipathic helix following its GNAT domain that may contribute to Golgi localization of hNaa60, and the beta7-beta8 hairpin adopted different conformations in the hNaa60(1-242) and hNaa60(1-199) crystal structures. Remarkably, we found that the side-chain of Phe 34 can influence the position of the coenzyme, indicating a new regulatory mechanism involving enzyme, co-factor and substrates interactions. Moreover, structural comparison and biochemical studies indicated that Tyr 97 and His 138 are key residues for catalytic reaction and that a non-conserved beta3-beta4 long loop participates in the regulation of hNaa60 activity.

-	~~Authors~~:	+	Structure and function of human Naa60 (NatF), a Golgi-localized bi-functional acetyltransferase.,Chen JY, Liu L, Cao CL, Li MJ, Tan K, Yang X, Yun CH Sci Rep. 2016 Aug 23;6:31425. doi: 10.1038/srep31425. PMID:27550639<ref>PMID:27550639</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5hh1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, J Y]]
		+	[[Category: Liu, L]]
		+	[[Category: Yun, C H]]
		+	[[Category: N-terminal acetylation]]
		+	[[Category: Nat]]
		+	[[Category: Protein modification]]
		+	[[Category: Transferase]]

OCA at 13:37, 15 May 2016

2016-05-15T13:37:13Z

←Older revision		Revision as of 13:37, 15 May 2016
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	The entry 5hh1 is ON HOLD until Paper Publication		The entry 5hh1 is ON HOLD until Paper Publication

-	Authors: ~~Chen, J.Y., Liu, L., Yun, C.H.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Liu, L]]
-	[[Category: Yun, C.H]]
-	[[Category: Chen, J.Y]]

OCA at 15:16, 10 February 2016

2016-02-10T15:16:14Z

←Older revision		Revision as of 15:16, 10 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hh1 is ON HOLD	+	The entry 5hh1 is ON HOLD until Paper Publication

	Authors: Chen, J.Y., Liu, L., Yun, C.H.		Authors: Chen, J.Y., Liu, L., Yun, C.H.

OCA: Protected "5hh1" [edit=sysop:move=sysop]

2016-01-20T15:25:17Z

Protected "5hh1" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:25, 20 January 2016

OCA: New page: '''Unreleased structure''' The entry 5hh1 is ON HOLD Authors: Chen, J.Y., Liu, L., Yun, C.H. Description: Category: Unreleased Structures Category: Liu, L [[Category: Yun, C.H...

2016-01-20T15:25:15Z

New page: '''Unreleased structure''' The entry 5hh1 is ON HOLD Authors: Chen, J.Y., Liu, L., Yun, C.H. Description: Category: Unreleased Structures Category: Liu, L [[Category: Yun, C.H...

New page

'''Unreleased structure'''

The entry 5hh1 is ON HOLD

Authors: Chen, J.Y., Liu, L., Yun, C.H.

Description:
[[Category: Unreleased Structures]]
[[Category: Liu, L]]
[[Category: Yun, C.H]]
[[Category: Chen, J.Y]]