5huk - Revision history

OCA at 10:54, 16 August 2023

2023-08-16T10:54:22Z

←Older revision		Revision as of 10:54, 16 August 2023
Line 1:		Line 1:

	==The crystal structure of neuraminidase from A/Northern pintail/Washington/40964/2014 influenza virus==		==The crystal structure of neuraminidase from A/Northern pintail/Washington/40964/2014 influenza virus==
-	<StructureSection load='5huk' size='340' side='right' caption='[[5huk]], [[Resolution\|resolution]] 2.45Å' scene=''>	+	<StructureSection load='5huk' size='340' side='right'caption='[[5huk]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5huk]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Influenza_a_virus_~~(a/~~northern_pintail~~/~~washington~~/40964/2014(~~h5n2~~)) Influenza a virus (a/~~northern~~ pintail/~~washington~~/40964/2014(~~h5n2~~))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HUK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5huk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Northern_pintail/Washington/40964/2014(H5N2)) Influenza A virus (A/Northern pintail/Washington/40964/2014(H5N2))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HUK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.45Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1589662 Influenza A virus (A/Northern pintail/Washington/40964/2014(H5N2))])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5huk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huk OCA], [https://pdbe.org/5huk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5huk RCSB], [https://www.ebi.ac.uk/pdbsum/5huk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5huk ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5huk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huk OCA], [~~http~~://pdbe.org/5huk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5huk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5huk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5huk ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/A0A0C4WXC5_9INFA A0A0C4WXC5_9INFA]] Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication (By similarity).[SAAS:SAAS00234776]	+	[https://www.uniprot.org/uniprot/A0A0C4WXC5_9INFA A0A0C4WXC5_9INFA] Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication (By similarity).[SAAS:SAAS00234776]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5huk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5huk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Neuraminidase 3D structures\|Neuraminidase 3D structures]]
		+	*[[O-GlcNAcase\|O-GlcNAcase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Exo-alpha-sialidase~~]]	+	[[Category: Large Structures]]
-	[[Category: Carney~~, P J~~]]	+	[[Category: Carney PJ]]
-	[[Category: Chang~~, J C~~]]	+	[[Category: Chang JC]]
-	[[Category: Guo, Z]]	+	[[Category: Guo Z]]
-	[[Category: Stevens, J]]	+	[[Category: Stevens J]]
-	[[Category: Yang, H]]	+	[[Category: Yang H]]
-	~~[[Category: H5nx]]~~	+
-	~~[[Category: Influenza virus]]~~	+
-	~~[[Category: Neuraminidase]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 13:32, 16 November 2017

2017-11-16T13:32:00Z

←Older revision		Revision as of 13:32, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='5huk' size='340' side='right' caption='[[5huk]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='5huk' size='340' side='right' caption='[[5huk]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5huk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HUK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5huk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus_(a/northern_pintail/washington/40964/2014(h5n2)) Influenza a virus (a/northern pintail/washington/40964/2014(h5n2))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HUK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1589662 Influenza A virus (A/Northern pintail/Washington/40964/2014(H5N2))])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5huk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huk OCA], [http://pdbe.org/5huk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5huk RCSB], [http://www.ebi.ac.uk/pdbsum/5huk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5huk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huk OCA], [http://pdbe.org/5huk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5huk RCSB], [http://www.ebi.ac.uk/pdbsum/5huk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5huk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 18:00, 10 May 2016

2016-05-10T18:00:09Z

←Older revision		Revision as of 18:00, 10 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5huk is ~~ON HOLD until Paper~~ Publication	+	==The crystal structure of neuraminidase from A/Northern pintail/Washington/40964/2014 influenza virus==
		+	<StructureSection load='5huk' size='340' side='right' caption='[[5huk]], [[Resolution\|resolution]] 2.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5huk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HUK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5huk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huk OCA], [http://pdbe.org/5huk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5huk RCSB], [http://www.ebi.ac.uk/pdbsum/5huk PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0A0C4WXC5_9INFA A0A0C4WXC5_9INFA]] Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication (By similarity).[SAAS:SAAS00234776]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During 2014, a subclade 2.3.4.4 HPAI A(H5N8) virus caused poultry outbreaks around the world. In late 2014/early 2015 the virus was detected in wild birds in Canada and the U.S. and these viruses also gave rise to reassortant progeny, composed of viral RNA segments (vRNAs) from both Eurasian and North America lineages. In particular, viruses were found with N1, N2 and N8 neuraminidase vRNAs, and are collectively referred to as H5Nx viruses. In the U. S., more than 48 million domestic birds have been affected. Here, we present a detailed structural and biochemical analysis of the surface antigens from H5N1, H5N2 and H5N8 in addition to a recent human H5N6 virus. Our results with recombinant hemagglutinin reveal that these viruses have a strict avian receptor binding preference, while recombinantly expressed neuraminidases are sensitive to FDA approved and investigational antivirals. Although H5Nx viruses currently pose a low risk to humans, it is important to maintain surveillance of these circulating viruses, and to continually assess future changes that may increase their pandemic potential. IMPORTANCE: The H5Nx viruses emerging in North America, Europe, and Asia are of great public health concern. Herein, we report a molecular and structural study of the major surface proteins from several H5Nx influenza viruses. Our results improve the understanding of these new viruses and provide important information on their receptor preference and susceptibility to antivirals, which is central to pandemic risk assessment.

-	~~Authors: Yang~~, H., Carney, ~~P.J.~~, Guo, Z., Chang, ~~J.C.~~, Stevens, J.	+	The molecular characterizations of surface proteins hemagglutinin and neuraminidase from recent H5Nx avian influenza viruses.,Yang H, Carney PJ, Mishin VP, Guo Z, Chang JC, Wentworth DE, Gubareva LV, Stevens J J Virol. 2016 Apr 6. pii: JVI.00180-16. PMID:27053557<ref>PMID:27053557</ref>

-	~~Description: The crystal structure~~ of ~~neuraminidase from A~~/~~Northern pintail~~/~~Washington~~/~~40964~~/~~2014 influenza virus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Yang, H~~]]	+	<div class="pdbe-citations 5huk" style="background-color:#fffaf0;"></div>
-	[[Category: Carney, P~~.J]]~~	+	== References ==
-	~~[[Category: Stevens,~~ J]]	+	<references/>
-	[[Category: Chang, J.C]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Exo-alpha-sialidase]]
		+	[[Category: Carney, P J]]
		+	[[Category: Chang, J C]]
	[[Category: Guo, Z]]		[[Category: Guo, Z]]
		+	[[Category: Stevens, J]]
		+	[[Category: Yang, H]]
		+	[[Category: H5nx]]
		+	[[Category: Influenza virus]]
		+	[[Category: Neuraminidase]]
		+	[[Category: Viral protein]]

OCA at 16:24, 26 February 2016

2016-02-26T16:24:16Z

←Older revision		Revision as of 16:24, 26 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5huk is ON HOLD	+	The entry 5huk is ON HOLD until Paper Publication

	Authors: Yang, H., Carney, P.J., Guo, Z., Chang, J.C., Stevens, J.		Authors: Yang, H., Carney, P.J., Guo, Z., Chang, J.C., Stevens, J.

OCA: Protected "5huk" [edit=sysop:move=sysop]

2016-02-03T14:22:23Z

Protected "5huk" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:22, 3 February 2016

OCA: New page: '''Unreleased structure''' The entry 5huk is ON HOLD Authors: Yang, H., Carney, P.J., Guo, Z., Chang, J.C., Stevens, J. Description: The crystal structure of neuraminidase from A/North...

2016-02-03T14:22:21Z

New page: '''Unreleased structure''' The entry 5huk is ON HOLD Authors: Yang, H., Carney, P.J., Guo, Z., Chang, J.C., Stevens, J. Description: The crystal structure of neuraminidase from A/North...

New page

'''Unreleased structure'''

The entry 5huk is ON HOLD

Authors: Yang, H., Carney, P.J., Guo, Z., Chang, J.C., Stevens, J.

Description: The crystal structure of neuraminidase from A/Northern pintail/Washington/40964/2014 influenza virus
[[Category: Unreleased Structures]]
[[Category: Yang, H]]
[[Category: Carney, P.J]]
[[Category: Stevens, J]]
[[Category: Chang, J.C]]
[[Category: Guo, Z]]