5i0w - Revision history

OCA at 08:19, 23 August 2023

2023-08-23T08:19:35Z

←Older revision		Revision as of 08:19, 23 August 2023
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	<StructureSection load='5i0w' size='340' side='right'caption='[[5i0w]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='5i0w' size='340' side='right'caption='[[5i0w]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i0w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Camjj Camjj~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I0W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5I0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i0w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni_81-176 Campylobacter jejuni subsp. jejuni 81-176]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I0W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lzn\|3lzn]], [[3lzo\|3lzo]], [[3lzq\|3lzq]], [[5i0v\|5i0v]], [[5i0x\|5i0x]], [[5i0y\|5i0y]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CJJ81176_1650 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354242 CAMJJ])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i0w OCA], [https://pdbe.org/5i0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i0w RCSB], [https://www.ebi.ac.uk/pdbsum/5i0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i0w ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5i0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i0w OCA], [~~http~~://pdbe.org/5i0w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5i0w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5i0w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5i0w ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0H3PA01_CAMJJ A0A0H3PA01_CAMJJ]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Campylobacter jejuni is a leading cause of food-borne gastrointestinal disease in humans and uropathogenic Escherichia coli is a leading cause of urinary tract infections. Both human pathogens harbour a homologous iron uptake system (termed cjFetM-P19 in C. jejuni and ecFetM-FetP in E. coli). Although these systems are important for growth under iron limitation, the mechanisms by which these systems function during iron transport remain undefined. The copper ions bound to P19 and FetP, the homologous periplasmic proteins, are coordinated in an uncommon penta-dentate manner involving a Met-Glu-His3 motif and exhibit positional plasticity. Here we demonstrate the function of the Met and Glu residues in modulating copper binding and controlling copper positioning through site-directed variants, binding assays, and crystal structures. Growth of C. jejuni strains with these p19 variants is impaired under iron limited conditions as compared to the wild-type strain. Additionally, an acidic residue-rich secondary site is required for binding iron and function in vivo. Finally, western blot analyses demonstrate direct and specific interactions between periplasmic P19 and FetP with the large periplasmic domain of their respective inner membrane transporters cjFetM and ecFetM.
		+
		+	A copper site is required for iron transport by the periplasmic proteins P19 and FetP.,Chan ACK, Lin H, Koch D, Grass G, Nies DH, Murphy MEP Metallomics. 2020 Oct 21;12(10):1530-1541. doi: 10.1039/d0mt00130a. PMID:32780051<ref>PMID:32780051</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5i0w" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[P19\|P19]]		*[[P19\|P19]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camjj~~]]	+	[[Category: Campylobacter jejuni subsp. jejuni 81-176]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chan~~, A C~~]]	+	[[Category: Chan AC]]
-	[[Category: Murphy~~, M E]]~~	+	[[Category: Murphy ME]]
-	~~[[Category: Iron transport]]~~	+
-	~~[[Category: Metal transport]]~~	+
-	~~[[Category: Mixed beta sandwich~~]]	+

OCA at 08:40, 27 May 2020

2020-05-27T08:40:18Z

←Older revision		Revision as of 08:40, 27 May 2020
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	==IRON AND COPPER-BOUND P19 FROM CAMPYLOBACTER JEJUNI UNDER REDUCING CONDITIONS==		==IRON AND COPPER-BOUND P19 FROM CAMPYLOBACTER JEJUNI UNDER REDUCING CONDITIONS==
-	<StructureSection load='5i0w' size='340' side='right' caption='[[5i0w]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='5i0w' size='340' side='right'caption='[[5i0w]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i0w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I0W OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5I0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i0w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camjj Camjj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I0W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5I0W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lzn\|3lzn]], [[3lzo\|3lzo]], [[3lzq\|3lzq]], [[5i0v\|5i0v]], [[5i0x\|5i0x]], [[5i0y\|5i0y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lzn\|3lzn]], [[3lzo\|3lzo]], [[3lzq\|3lzq]], [[5i0v\|5i0v]], [[5i0x\|5i0x]], [[5i0y\|5i0y]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5i0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i0w OCA], [http://pdbe.org/5i0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i0w RCSB], [http://www.ebi.ac.uk/pdbsum/5i0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i0w ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CJJ81176_1650 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354242 CAMJJ])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5i0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i0w OCA], [http://pdbe.org/5i0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i0w RCSB], [http://www.ebi.ac.uk/pdbsum/5i0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i0w ProSAT]</span></td></tr>
	</table>		</table>
		+
		+	==See Also==
		+	*[[P19\|P19]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camjj]]
		+	[[Category: Large Structures]]
	[[Category: Chan, A C]]		[[Category: Chan, A C]]
	[[Category: Murphy, M E]]		[[Category: Murphy, M E]]

OCA at 09:23, 10 March 2017

2017-03-10T09:23:49Z

←Older revision		Revision as of 09:23, 10 March 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5i0w is ~~ON HOLD until Paper Publication~~	+	==IRON AND COPPER-BOUND P19 FROM CAMPYLOBACTER JEJUNI UNDER REDUCING CONDITIONS==
-		+	<StructureSection load='5i0w' size='340' side='right' caption='[[5i0w]], [[Resolution\|resolution]] 1.55Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5i0w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I0W FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lzn\|3lzn]], [[3lzo\|3lzo]], [[3lzq\|3lzq]], [[5i0v\|5i0v]], [[5i0x\|5i0x]], [[5i0y\|5i0y]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i0w OCA], [http://pdbe.org/5i0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i0w RCSB], [http://www.ebi.ac.uk/pdbsum/5i0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i0w ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chan, A C]]
		+	[[Category: Murphy, M E]]
		+	[[Category: Iron transport]]
		+	[[Category: Metal transport]]
		+	[[Category: Mixed beta sandwich]]

OCA at 14:04, 15 May 2016

2016-05-15T14:04:13Z

←Older revision		Revision as of 14:04, 15 May 2016
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	The entry 5i0w is ON HOLD until Paper Publication		The entry 5i0w is ON HOLD until Paper Publication

-	Authors: ~~Chan, A.C., Murphy, M.E.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Chan, A.C]]
-	[[Category: Murphy, M.E]]

OCA at 01:24, 21 February 2016

2016-02-21T01:24:38Z

←Older revision		Revision as of 01:24, 21 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5i0w is ON HOLD	+	The entry 5i0w is ON HOLD until Paper Publication

	Authors: Chan, A.C., Murphy, M.E.		Authors: Chan, A.C., Murphy, M.E.

OCA: Protected "5i0w" [edit=sysop:move=sysop]

2016-02-10T16:02:36Z

Protected "5i0w" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:02, 10 February 2016

OCA: New page: '''Unreleased structure''' The entry 5i0w is ON HOLD Authors: Chan, A.C., Murphy, M.E. Description: Category: Unreleased Structures Category: Chan, A.C [[Category: Murphy, M.E...

2016-02-10T16:02:34Z

New page: '''Unreleased structure''' The entry 5i0w is ON HOLD Authors: Chan, A.C., Murphy, M.E. Description: Category: Unreleased Structures Category: Chan, A.C [[Category: Murphy, M.E...

New page

'''Unreleased structure'''

The entry 5i0w is ON HOLD

Authors: Chan, A.C., Murphy, M.E.

Description:
[[Category: Unreleased Structures]]
[[Category: Chan, A.C]]
[[Category: Murphy, M.E]]