5i71 - Revision history

OCA at 08:25, 23 August 2023

2023-08-23T08:25:27Z

←Older revision		Revision as of 08:25, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='5i71' size='340' side='right'caption='[[5i71]], [[Resolution\|resolution]] 3.15Å' scene=''>		<StructureSection load='5i71' size='340' side='right'caption='[[5i71]], [[Resolution\|resolution]] 3.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i71]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~] and [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I71 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5I71 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i71]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I71 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I71 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=68P:(1S)-1-[3-(DIMETHYLAMINO)PROPYL]-1-(4-FLUOROPHENYL)-1,3-DIHYDRO-2-BENZOFURAN-5-CARBONITRILE'>68P</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=HEX:HEXANE'>HEX</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.15Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5i75\|5i75]], [[5i74\|5i74]], [[5i73\|5i73]], [[5i6z\|5i6z]], [[5i6x\|5i6x]], [[5i66\|5i66]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=68P:(1S)-1-[3-(DIMETHYLAMINO)PROPYL]-1-(4-FLUOROPHENYL)-1,3-DIHYDRO-2-BENZOFURAN-5-CARBONITRILE'>68P</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=HEX:HEXANE'>HEX</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC6A4, HTT, SERT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i71 OCA], [https://pdbe.org/5i71 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i71 RCSB], [https://www.ebi.ac.uk/pdbsum/5i71 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i71 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5i71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i71 OCA], [~~http~~://pdbe.org/5i71 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5i71 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5i71 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5i71 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SC6A4_HUMAN SC6A4_HUMAN]] Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.<ref>PMID:17506858</ref> <ref>PMID:18227069</ref> <ref>PMID:19270731</ref>	+	[https://www.uniprot.org/uniprot/SC6A4_HUMAN SC6A4_HUMAN] Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.<ref>PMID:17506858</ref> <ref>PMID:18227069</ref> <ref>PMID:19270731</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
	*[[Serotonin Transporter\|Serotonin Transporter]]		*[[Serotonin Transporter\|Serotonin Transporter]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Coleman~~, J A~~]]	+	[[Category: Coleman JA]]
-	[[Category: Gouaux, E]]	+	[[Category: Gouaux E]]
-	[[Category: Green~~, E M]]~~	+	[[Category: Green EM]]
-	~~[[Category: Membrane protein~~]]	+

OCA at 07:03, 4 March 2020

2020-03-04T07:03:50Z

←Older revision		Revision as of 07:03, 4 March 2020
Line 1:		Line 1:

	==X-ray structure of the ts3 human serotonin transporter complexed with s-citalopram at the central site==		==X-ray structure of the ts3 human serotonin transporter complexed with s-citalopram at the central site==
-	<StructureSection load='5i71' size='340' side='right' caption='[[5i71]], [[Resolution\|resolution]] 3.15Å' scene=''>	+	<StructureSection load='5i71' size='340' side='right'caption='[[5i71]], [[Resolution\|resolution]] 3.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5i71]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I71 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5i71]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I71 FirstGlance]. <br>
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[3D structures ~~of antibody~~\|3D structures ~~of antibody~~]]	+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Serotonin Transporter\|Serotonin Transporter]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Coleman, J A]]		[[Category: Coleman, J A]]

OCA at 13:34, 16 November 2017

2017-11-16T13:34:39Z

←Older revision		Revision as of 13:34, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='5i71' size='340' side='right' caption='[[5i71]], [[Resolution\|resolution]] 3.15Å' scene=''>		<StructureSection load='5i71' size='340' side='right' caption='[[5i71]], [[Resolution\|resolution]] 3.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5i71]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I71 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5i71]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I71 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=68P:(1S)-1-[3-(DIMETHYLAMINO)PROPYL]-1-(4-FLUOROPHENYL)-1,3-DIHYDRO-2-BENZOFURAN-5-CARBONITRILE'>68P</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=HEX:HEXANE'>HEX</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=68P:(1S)-1-[3-(DIMETHYLAMINO)PROPYL]-1-(4-FLUOROPHENYL)-1,3-DIHYDRO-2-BENZOFURAN-5-CARBONITRILE'>68P</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=HEX:HEXANE'>HEX</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5i75\|5i75]], [[5i74\|5i74]], [[5i73\|5i73]], [[5i6z\|5i6z]], [[5i6x\|5i6x]], [[5i66\|5i66]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5i75\|5i75]], [[5i74\|5i74]], [[5i73\|5i73]], [[5i6z\|5i6z]], [[5i6x\|5i6x]], [[5i66\|5i66]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i71 OCA], [http://pdbe.org/5i71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i71 RCSB], [http://www.ebi.ac.uk/pdbsum/5i71 PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC6A4, HTT, SERT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i71 OCA], [http://pdbe.org/5i71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i71 RCSB], [http://www.ebi.ac.uk/pdbsum/5i71 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i71 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 5i71" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5i71" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[3D structures of antibody\|3D structures of antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Coleman, J A]]		[[Category: Coleman, J A]]
	[[Category: Gouaux, E]]		[[Category: Gouaux, E]]
	[[Category: Green, E M]]		[[Category: Green, E M]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 16:09, 10 May 2016

2016-05-10T16:09:52Z

←Older revision		Revision as of 16:09, 10 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5i71 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==X-ray structure of the ts3 human serotonin transporter complexed with s-citalopram at the central site==
		+	<StructureSection load='5i71' size='340' side='right' caption='[[5i71]], [[Resolution\|resolution]] 3.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5i71]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I71 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=68P:(1S)-1-[3-(DIMETHYLAMINO)PROPYL]-1-(4-FLUOROPHENYL)-1,3-DIHYDRO-2-BENZOFURAN-5-CARBONITRILE'>68P</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=HEX:HEXANE'>HEX</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5i75\|5i75]], [[5i74\|5i74]], [[5i73\|5i73]], [[5i6z\|5i6z]], [[5i6x\|5i6x]], [[5i66\|5i66]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i71 OCA], [http://pdbe.org/5i71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i71 RCSB], [http://www.ebi.ac.uk/pdbsum/5i71 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SC6A4_HUMAN SC6A4_HUMAN]] Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.<ref>PMID:17506858</ref> <ref>PMID:18227069</ref> <ref>PMID:19270731</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The serotonin transporter (SERT) terminates serotonergic signalling through the sodium- and chloride-dependent reuptake of neurotransmitter into presynaptic neurons. SERT is a target for antidepressant and psychostimulant drugs, which block reuptake and prolong neurotransmitter signalling. Here we report X-ray crystallographic structures of human SERT at 3.15 A resolution bound to the antidepressants (S)-citalopram or paroxetine. Antidepressants lock SERT in an outward-open conformation by lodging in the central binding site, located between transmembrane helices 1, 3, 6, 8 and 10, directly blocking serotonin binding. We further identify the location of an allosteric site in the complex as residing at the periphery of the extracellular vestibule, interposed between extracellular loops 4 and 6 and transmembrane helices 1, 6, 10 and 11. Occupancy of the allosteric site sterically hinders ligand unbinding from the central site, providing an explanation for the action of (S)-citalopram as an allosteric ligand. These structures define the mechanism of antidepressant action in SERT, and provide blueprints for future drug design.

-	~~Authors: Coleman, J.A~~., Green, E.M.~~, Gouaux, E~~.	+	X-ray structures and mechanism of the human serotonin transporter.,Coleman JA, Green EM, Gouaux E Nature. 2016 Apr 21;532(7599):334-9. doi: 10.1038/nature17629. Epub 2016 Apr 6. PMID:27049939<ref>PMID:27049939</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5i71" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Coleman, J A]]
	[[Category: Gouaux, E]]		[[Category: Gouaux, E]]
-	[[Category: Green, E.M]]	+	[[Category: Green, E M]]
-	[[Category: ~~Coleman, J.A~~]]	+	[[Category: Membrane protein]]

OCA at 01:56, 10 March 2016

2016-03-10T01:56:18Z

←Older revision		Revision as of 01:56, 10 March 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5i71 is ON HOLD	+	The entry 5i71 is ON HOLD until Paper Publication

	Authors: Coleman, J.A., Green, E.M., Gouaux, E.		Authors: Coleman, J.A., Green, E.M., Gouaux, E.

OCA: Protected "5i71" [edit=sysop:move=sysop]

2016-02-26T16:54:38Z

Protected "5i71" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:54, 26 February 2016

OCA: New page: '''Unreleased structure''' The entry 5i71 is ON HOLD Authors: Coleman, J.A., Green, E.M., Gouaux, E. Description: Category: Unreleased Structures Category: Gouaux, E [[Categor...

2016-02-26T16:54:36Z

New page: '''Unreleased structure''' The entry 5i71 is ON HOLD Authors: Coleman, J.A., Green, E.M., Gouaux, E. Description: Category: Unreleased Structures Category: Gouaux, E [[Categor...

New page

'''Unreleased structure'''

The entry 5i71 is ON HOLD

Authors: Coleman, J.A., Green, E.M., Gouaux, E.

Description:
[[Category: Unreleased Structures]]
[[Category: Gouaux, E]]
[[Category: Green, E.M]]
[[Category: Coleman, J.A]]