5icw - Revision history

OCA at 13:46, 30 August 2023

2023-08-30T13:46:12Z

←Older revision		Revision as of 13:46, 30 August 2023
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	<StructureSection load='5icw' size='340' side='right'caption='[[5icw]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='5icw' size='340' side='right'caption='[[5icw]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5icw]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ICW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5ICW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5icw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ICW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ICW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=COA:COENZYME+A'>COA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.951Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~NAA60, HAT4, NAT15, UNQ2771/PRO7155 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5icw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5icw OCA], [~~http~~://pdbe.org/5icw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5icw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5icw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5icw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5icw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5icw OCA], [https://pdbe.org/5icw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5icw RCSB], [https://www.ebi.ac.uk/pdbsum/5icw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5icw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NAA60_HUMAN NAA60_HUMAN]] Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase.<ref>PMID:21750686</ref> <ref>PMID:21981917</ref>	+	[https://www.uniprot.org/uniprot/NAA60_HUMAN NAA60_HUMAN] Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase.<ref>PMID:21750686</ref> <ref>PMID:21981917</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Arnesen, T]]	+	[[Category: Arnesen T]]
-	[[Category: Magin~~, R S~~]]	+	[[Category: Magin RS]]
-	[[Category: Marmorstein, R]]	+	[[Category: Marmorstein R]]
-	[[Category: Stove~~, S I]]~~	+	[[Category: Stove SI]]
-	~~[[Category: Acetylation]]~~	+
-	~~[[Category: Gnat]]~~	+
-	~~[[Category: Nat]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:13, 3 June 2020

2020-06-03T07:13:35Z

←Older revision		Revision as of 07:13, 3 June 2020
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	==Crystal structure of human NatF (hNaa60) homodimer bound to Coenzyme A==		==Crystal structure of human NatF (hNaa60) homodimer bound to Coenzyme A==
-	<StructureSection load='5icw' size='340' side='right' caption='[[5icw]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='5icw' size='340' side='right'caption='[[5icw]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5icw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ICW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ICW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5icw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ICW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ICW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5icw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5icw OCA], [http://pdbe.org/5icw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5icw RCSB], [http://www.ebi.ac.uk/pdbsum/5icw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5icw ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NAA60, HAT4, NAT15, UNQ2771/PRO7155 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5icw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5icw OCA], [http://pdbe.org/5icw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5icw RCSB], [http://www.ebi.ac.uk/pdbsum/5icw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5icw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Arnesen, T]]		[[Category: Arnesen, T]]
	[[Category: Magin, R S]]		[[Category: Magin, R S]]

OCA at 17:51, 12 July 2016

2016-07-12T17:51:21Z

←Older revision		Revision as of 17:51, 12 July 2016
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[5icw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ICW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ICW FirstGlance]. <br>		<table><tr><td colspan='2'>[[5icw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ICW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ICW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5icw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5icw OCA], [http://pdbe.org/5icw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5icw RCSB], [http://www.ebi.ac.uk/pdbsum/5icw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5icw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5icw OCA], [http://pdbe.org/5icw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5icw RCSB], [http://www.ebi.ac.uk/pdbsum/5icw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5icw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/NAA60_HUMAN NAA60_HUMAN]] Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase.<ref>PMID:21750686</ref> <ref>PMID:21981917</ref>		[[http://www.uniprot.org/uniprot/NAA60_HUMAN NAA60_HUMAN]] Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase.<ref>PMID:21750686</ref> <ref>PMID:21981917</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	N-Terminal acetylation is a common and important protein modification catalyzed by N-terminal acetyltransferases (NATs). Six human NATs (NatA-NatF) contain one catalytic subunit each, Naa10 to Naa60, respectively. In contrast to the ribosome-associated NatA to NatE, NatF/Naa60 specifically associates with Golgi membranes and acetylates transmembrane proteins. To gain insight into the molecular basis for the function of Naa60, we developed an Naa60 bisubstrate CoA-peptide conjugate inhibitor, determined its X-ray structure when bound to CoA and inhibitor, and carried out biochemical experiments. We show that Naa60 adapts an overall fold similar to that of the catalytic subunits of ribosome-associated NATs, but with the addition of two novel elongated loops that play important roles in substrate-specific binding. One of these loops mediates a dimer to monomer transition upon substrate-specific binding. Naa60 employs a catalytic mechanism most similar to Naa50. Collectively, these data reveal the molecular basis for Naa60-specific acetyltransferase activity with implications for its Golgi-specific functions.
		+
		+	Crystal Structure of the Golgi-Associated Human Nalpha-Acetyltransferase 60 Reveals the Molecular Determinants for Substrate-Specific Acetylation.,Stove SI, Magin RS, Foyn H, Haug BE, Marmorstein R, Arnesen T Structure. 2016 Jul 6;24(7):1044-56. doi: 10.1016/j.str.2016.04.020. Epub 2016, Jun 16. PMID:27320834<ref>PMID:27320834</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5icw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:28, 23 June 2016

2016-06-23T23:28:11Z

←Older revision		Revision as of 23:28, 23 June 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5icw ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of human NatF (hNaa60) homodimer bound to Coenzyme A==
-		+	<StructureSection load='5icw' size='340' side='right' caption='[[5icw]], [[Resolution\|resolution]] 1.95Å' scene=''>
-	~~Authors~~: ~~Stove~~, S.I., ~~Magin, R~~.S., ~~Marmorstein~~, R., ~~Arnesen, T~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5icw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ICW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ICW FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of ~~human NatF~~ (~~hNaa60~~) ~~homodimer bound to Coenzyme A~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5icw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5icw OCA], [http://pdbe.org/5icw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5icw RCSB], [http://www.ebi.ac.uk/pdbsum/5icw PDBsum]</span></td></tr>
-	[[Category: ~~Stove~~, S.I]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NAA60_HUMAN NAA60_HUMAN]] Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase.<ref>PMID:21750686</ref> <ref>PMID:21981917</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Arnesen, T]]
		+	[[Category: Magin, R S]]
	[[Category: Marmorstein, R]]		[[Category: Marmorstein, R]]
-	[[Category: ~~Arnesen~~, T]]	+	[[Category: Stove, S I]]
-	[[Category: ~~Magin, R.S~~]]	+	[[Category: Acetylation]]
		+	[[Category: Gnat]]
		+	[[Category: Nat]]
		+	[[Category: Transferase]]

OCA at 00:23, 11 May 2016

2016-05-11T00:23:53Z

←Older revision		Revision as of 00:23, 11 May 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5icw is ON HOLD	+	The entry 5icw is ON HOLD until Paper Publication

	Authors: Stove, S.I., Magin, R.S., Marmorstein, R., Arnesen, T.		Authors: Stove, S.I., Magin, R.S., Marmorstein, R., Arnesen, T.

OCA: Protected "5icw" [edit=sysop:move=sysop]

2016-03-02T13:45:36Z

Protected "5icw" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:45, 2 March 2016

OCA: New page: '''Unreleased structure''' The entry 5icw is ON HOLD Authors: Stove, S.I., Magin, R.S., Marmorstein, R., Arnesen, T. Description: Crystal structure of human NatF (hNaa60) homodimer bou...

2016-03-02T13:45:35Z

New page: '''Unreleased structure''' The entry 5icw is ON HOLD Authors: Stove, S.I., Magin, R.S., Marmorstein, R., Arnesen, T. Description: Crystal structure of human NatF (hNaa60) homodimer bou...

New page

'''Unreleased structure'''

The entry 5icw is ON HOLD

Authors: Stove, S.I., Magin, R.S., Marmorstein, R., Arnesen, T.

Description: Crystal structure of human NatF (hNaa60) homodimer bound to Coenzyme A
[[Category: Unreleased Structures]]
[[Category: Stove, S.I]]
[[Category: Marmorstein, R]]
[[Category: Arnesen, T]]
[[Category: Magin, R.S]]