5igt - Revision history

OCA at 13:52, 30 August 2023

2023-08-30T13:52:05Z

←Older revision		Revision as of 13:52, 30 August 2023
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	<StructureSection load='5igt' size='340' side='right'caption='[[5igt]], [[Resolution\|resolution]] 1.39Å' scene=''>		<StructureSection load='5igt' size='340' side='right'caption='[[5igt]], [[Resolution\|resolution]] 1.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5igt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IGT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5igt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IGT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene>, <scene name='pdbligand=GMP:GUANOSINE'>GMP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.39Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5igh\|5igh]], [[5igi\|5igi]], [[5igj\|5igj]], [[5igp\|5igp]], [[5igr\|5igr]], [[5igs\|5igs]], [[5igu\|5igu]], [[5igv\|5igv]], [[5igw\|5igw]], [[5igy\|5igy]], [[5igz\|5igz]], [[5ih0\|5ih0]], [[5ih1\|5ih1]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene>, <scene name='pdbligand=GMP:GUANOSINE'>GMP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mphA, mph(A), mph2, AM267_25240, AM268_24740, AN205_25580, AN669_16770, ASU34_20250, AZ95_0038, ECONIH1_26770, ERS085366_04054, ERS085367_04848, ERS139269_04809, ERS150873_04753, ETN48_p0083, orf00017, pCTXM123_C0996_11, pKC394-009, SK74_04859, SK86_03516, UN86_19875 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5igt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igt OCA], [https://pdbe.org/5igt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5igt RCSB], [https://www.ebi.ac.uk/pdbsum/5igt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5igt ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5igt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igt OCA], [~~http~~://pdbe.org/5igt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5igt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5igt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5igt ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q47396_ECOLX Q47396_ECOLX]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Berghuis~~, A M~~]]	+	[[Category: Berghuis AM]]
-	[[Category: Fong~~, D H]]~~	+	[[Category: Fong DH]]
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Macrolide phosphotransferase]]~~	+
-	~~[[Category: Transferase-antibiotic complex~~]]	+

OCA at 08:04, 8 January 2020

2020-01-08T08:04:54Z

←Older revision		Revision as of 08:04, 8 January 2020
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	==Macrolide 2'-phosphotransferase type I - complex with guanosine and erythromycin==		==Macrolide 2'-phosphotransferase type I - complex with guanosine and erythromycin==
-	<StructureSection load='5igt' size='340' side='right' caption='[[5igt]], [[Resolution\|resolution]] 1.39Å' scene=''>	+	<StructureSection load='5igt' size='340' side='right'caption='[[5igt]], [[Resolution\|resolution]] 1.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5igt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IGT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5igt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IGT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene>, <scene name='pdbligand=GMP:GUANOSINE'>GMP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene>, <scene name='pdbligand=GMP:GUANOSINE'>GMP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5igh\|5igh]], [[5igi\|5igi]], [[5igj\|5igj]], [[5igp\|5igp]], [[5igr\|5igr]], [[5igs\|5igs]], [[5igu\|5igu]], [[5igv\|5igv]], [[5igw\|5igw]], [[5igy\|5igy]], [[5igz\|5igz]], [[5ih0\|5ih0]], [[5ih1\|5ih1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5igh\|5igh]], [[5igi\|5igi]], [[5igj\|5igj]], [[5igp\|5igp]], [[5igr\|5igr]], [[5igs\|5igs]], [[5igu\|5igu]], [[5igv\|5igv]], [[5igw\|5igw]], [[5igy\|5igy]], [[5igz\|5igz]], [[5ih0\|5ih0]], [[5ih1\|5ih1]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mphA, mph(A), mph2, AM267_25240, AM268_24740, AN205_25580, AN669_16770, ASU34_20250, AZ95_0038, ECONIH1_26770, ERS085366_04054, ERS085367_04848, ERS139269_04809, ERS150873_04753, ETN48_p0083, orf00017, pCTXM123_C0996_11, pKC394-009, SK74_04859, SK86_03516, UN86_19875 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5igt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igt OCA], [http://pdbe.org/5igt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5igt RCSB], [http://www.ebi.ac.uk/pdbsum/5igt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5igt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5igt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igt OCA], [http://pdbe.org/5igt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5igt RCSB], [http://www.ebi.ac.uk/pdbsum/5igt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5igt ProSAT]</span></td></tr>
	</table>		</table>
Line 19:		Line 20:

	==See Also==		==See Also==
-	*[[Phosphotransferase\|Phosphotransferase]]	+	*[[Phosphotransferase 3D structures\|Phosphotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Berghuis, A M]]		[[Category: Berghuis, A M]]
	[[Category: Fong, D H]]		[[Category: Fong, D H]]

OCA at 07:05, 6 October 2017

2017-10-06T07:05:53Z

←Older revision		Revision as of 07:05, 6 October 2017
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 5igt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5igt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphotransferase\|Phosphotransferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:30, 4 May 2017

2017-05-04T06:30:46Z

←Older revision		Revision as of 06:30, 4 May 2017
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5igt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igt OCA], [http://pdbe.org/5igt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5igt RCSB], [http://www.ebi.ac.uk/pdbsum/5igt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5igt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5igt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igt OCA], [http://pdbe.org/5igt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5igt RCSB], [http://www.ebi.ac.uk/pdbsum/5igt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5igt ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The macrolides are a class of antibiotic, characterized by a large macrocyclic lactone ring that can be inactivated by macrolide phosphotransferase enzymes. We present structures for MPH(2')-I and MPH(2')-II in the apo state, and in complex with GTP analogs and six different macrolides. These represent the first structures from the two main classes of macrolide phosphotransferases. The structures show that the enzymes are related to the aminoglycoside phosphotransferases, but are distinguished from them by the presence of a large interdomain linker that contributes to an expanded antibiotic binding pocket. This pocket is largely hydrophobic, with a negatively charged patch located at a conserved aspartate residue, rationalizing the broad-spectrum resistance conferred by the enzymes. Complementary mutation studies provide insights into factors governing substrate specificity. A comparison with macrolides bound to their natural target, the 50S ribosome, suggests avenues for next-generation antibiotic development.
		+
		+	Structural Basis for Kinase-Mediated Macrolide Antibiotic Resistance.,Fong DH, Burk DL, Blanchet J, Yan AY, Berghuis AM Structure. 2017 May 2;25(5):750-761.e5. doi: 10.1016/j.str.2017.03.007. Epub 2017, Apr 13. PMID:28416110<ref>PMID:28416110</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5igt" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:57, 27 April 2017

2017-04-27T13:57:08Z

←Older revision		Revision as of 13:57, 27 April 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5igt is ~~ON HOLD until May 31 2018~~	+	==Macrolide 2'-phosphotransferase type I - complex with guanosine and erythromycin==
-		+	<StructureSection load='5igt' size='340' side='right' caption='[[5igt]], [[Resolution\|resolution]] 1.39Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5igt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IGT FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene>, <scene name='pdbligand=GMP:GUANOSINE'>GMP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5igh\|5igh]], [[5igi\|5igi]], [[5igj\|5igj]], [[5igp\|5igp]], [[5igr\|5igr]], [[5igs\|5igs]], [[5igu\|5igu]], [[5igv\|5igv]], [[5igw\|5igw]], [[5igy\|5igy]], [[5igz\|5igz]], [[5ih0\|5ih0]], [[5ih1\|5ih1]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5igt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igt OCA], [http://pdbe.org/5igt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5igt RCSB], [http://www.ebi.ac.uk/pdbsum/5igt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5igt ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Berghuis, A M]]
		+	[[Category: Fong, D H]]
		+	[[Category: Kinase]]
		+	[[Category: Macrolide phosphotransferase]]
		+	[[Category: Transferase-antibiotic complex]]

OCA at 09:34, 9 March 2017

2017-03-09T09:34:46Z

←Older revision		Revision as of 09:34, 9 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5igt is ON HOLD until ~~Paper Publication~~	+	The entry 5igt is ON HOLD until May 31 2018

	Authors:		Authors:

OCA at 14:25, 15 May 2016

2016-05-15T14:25:04Z

←Older revision		Revision as of 14:25, 15 May 2016
Line 3:		Line 3:
	The entry 5igt is ON HOLD until Paper Publication		The entry 5igt is ON HOLD until Paper Publication

-	Authors: ~~Berghuis, A.M., Fong, D.H.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Berghuis, A.M]]
-	[[Category: Fong, D.H]]

OCA at 00:29, 11 May 2016

2016-05-11T00:29:19Z

←Older revision		Revision as of 00:29, 11 May 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5igt is ON HOLD	+	The entry 5igt is ON HOLD until Paper Publication

	Authors: Berghuis, A.M., Fong, D.H.		Authors: Berghuis, A.M., Fong, D.H.

OCA: Protected "5igt" [edit=sysop:move=sysop]

2016-03-10T02:17:39Z

Protected "5igt" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:17, 10 March 2016

OCA: New page: '''Unreleased structure''' The entry 5igt is ON HOLD Authors: Berghuis, A.M., Fong, D.H. Description: Category: Unreleased Structures Category: Berghuis, A.M [[Category: Fong,...

2016-03-10T02:17:38Z

New page: '''Unreleased structure''' The entry 5igt is ON HOLD Authors: Berghuis, A.M., Fong, D.H. Description: Category: Unreleased Structures Category: Berghuis, A.M [[Category: Fong,...

New page

'''Unreleased structure'''

The entry 5igt is ON HOLD

Authors: Berghuis, A.M., Fong, D.H.

Description:
[[Category: Unreleased Structures]]
[[Category: Berghuis, A.M]]
[[Category: Fong, D.H]]