5iia - Revision history

OCA at 13:53, 30 August 2023

2023-08-30T13:53:57Z

←Older revision		Revision as of 13:53, 30 August 2023
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	==Crystal structure of red abalone egg VERL repeat 3 in complex with sperm lysin at 1.7 A resolution (crystal form I)==		==Crystal structure of red abalone egg VERL repeat 3 in complex with sperm lysin at 1.7 A resolution (crystal form I)==
-	<StructureSection load='5iia' size='340' side='right' caption='[[5iia]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='5iia' size='340' side='right'caption='[[5iia]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5iia]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~California_red_abalone California red abalone~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IIA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IIA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5iia]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Haliotis_rufescens Haliotis rufescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IIA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lis\|1lis]]~~, [[~~2lis~~\|2lis]], [[1lyn\|1lyn]], [[2lyn\|2lyn]], [[3d4c\|3d4c]], [[3d4g\|3d4g]], [[3ef7\|3ef7]], [[3nk3\|3nk3]], [[3nk4\|3nk4]], [[5ii5\|5ii5]], [[5ii4\|5ii4]], [[5iic\|5iic]], [[5ii7\|5ii7]], [[5ii8\|5ii8]], [[5ii9\|5ii9]], [[5iib\|5iib]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~VERL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=6454 California red abalone])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iia OCA], [https://pdbe.org/5iia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iia RCSB], [https://www.ebi.ac.uk/pdbsum/5iia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iia ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5iia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iia OCA], [~~http~~://pdbe.org/5iia PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5iia RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5iia PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5iia ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ELYS_HALRU ELYS_HALRU]] Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass.	+	[https://www.uniprot.org/uniprot/ELYS_HALRU ELYS_HALRU] Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5iia" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5iia" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysin 3D structures\|Lysin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~California red abalone~~]]	+	[[Category: Haliotis rufescens]]
-	[[Category: ~~Al-Hosseini, H Sadat]]~~	+	[[Category: Large Structures]]
-	~~[[Category: Jovine, L]]~~	+	[[Category: De Sanctis D]]
-	~~[[Category: Nishimura, K]]~~	+	[[Category: Jovine L]]
-	~~[[Category: Raj, I~~]]	+	[[Category: Nishimura K]]
-	[[Category: Sanctis, D De]]	+	[[Category: Raj I]]
-	[[Category: ~~Cell adhesion~~]]	+	[[Category: Sadat Al-Hosseini H]]
-	[[Category: ~~Egg coat~~]]	+
-	[[Category: ~~Egg coat penetration~~]]	+
-	[[Category: ~~Egg~~-~~sperm interaction]]~~	+
-	~~[[Category: Fertilization]]~~	+
-	~~[[Category: Gamete recognition]]~~	+
-	~~[[Category: Sperm acrosome]]~~	+
-	~~[[Category: Sperm receptor]]~~	+
-	~~[[Category: Vitelline envelope]]~~	+
-	~~[[Category: Zp domain]]~~	+
-	~~[[Category: Zp-n domain~~]]	+

OCA at 07:24, 29 November 2017

2017-11-29T07:24:57Z

←Older revision		Revision as of 07:24, 29 November 2017
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	<StructureSection load='5iia' size='340' side='right' caption='[[5iia]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5iia' size='340' side='right' caption='[[5iia]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5iia]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IIA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IIA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5iia]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/California_red_abalone California red abalone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IIA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IIA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lis\|1lis]], [[2lis\|2lis]], [[1lyn\|1lyn]], [[2lyn\|2lyn]], [[3d4c\|3d4c]], [[3d4g\|3d4g]], [[3ef7\|3ef7]], [[3nk3\|3nk3]], [[3nk4\|3nk4]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lis\|1lis]], [[2lis\|2lis]], [[1lyn\|1lyn]], [[2lyn\|2lyn]], [[3d4c\|3d4c]], [[3d4g\|3d4g]], [[3ef7\|3ef7]], [[3nk3\|3nk3]], [[3nk4\|3nk4]], [[5ii5\|5ii5]], [[5ii4\|5ii4]], [[5iic\|5iic]], [[5ii7\|5ii7]], [[5ii8\|5ii8]], [[5ii9\|5ii9]], [[5iib\|5iib]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VERL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6454 California red abalone])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iia OCA], [http://pdbe.org/5iia PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iia RCSB], [http://www.ebi.ac.uk/pdbsum/5iia PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iia ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iia OCA], [http://pdbe.org/5iia PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iia RCSB], [http://www.ebi.ac.uk/pdbsum/5iia PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iia ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: California red abalone]]
	[[Category: Al-Hosseini, H Sadat]]		[[Category: Al-Hosseini, H Sadat]]
	[[Category: Jovine, L]]		[[Category: Jovine, L]]

OCA at 12:42, 6 November 2017

2017-11-06T12:42:04Z

←Older revision		Revision as of 12:42, 6 November 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5iia" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5iia" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Lysin\|Lysin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:16, 3 August 2017

2017-08-03T09:16:20Z

←Older revision		Revision as of 09:16, 3 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5iia is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of red abalone egg VERL repeat 3 in complex with sperm lysin at 1.7 A resolution (crystal form I)==
		+	<StructureSection load='5iia' size='340' side='right' caption='[[5iia]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5iia]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IIA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IIA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lis\|1lis]], [[2lis\|2lis]], [[1lyn\|1lyn]], [[2lyn\|2lyn]], [[3d4c\|3d4c]], [[3d4g\|3d4g]], [[3ef7\|3ef7]], [[3nk3\|3nk3]], [[3nk4\|3nk4]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iia OCA], [http://pdbe.org/5iia PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iia RCSB], [http://www.ebi.ac.uk/pdbsum/5iia PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iia ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ELYS_HALRU ELYS_HALRU]] Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Recognition between sperm and the egg surface marks the beginning of life in all sexually reproducing organisms. This fundamental biological event depends on the species-specific interaction between rapidly evolving counterpart molecules on the gametes. We report biochemical, crystallographic, and mutational studies of domain repeats 1-3 of invertebrate egg coat protein VERL and their interaction with cognate sperm protein lysin. VERL repeats fold like the functionally essential N-terminal repeat of mammalian sperm receptor ZP2, whose structure is also described here. Whereas sequence-divergent repeat 1 does not bind lysin, repeat 3 binds it non-species specifically via a high-affinity, largely hydrophobic interface. Due to its intermediate binding affinity, repeat 2 selectively interacts with lysin from the same species. Exposure of a highly positively charged surface of VERL-bound lysin suggests that complex formation both disrupts the organization of egg coat filaments and triggers their electrostatic repulsion, thereby opening a hole for sperm penetration and fusion.

-	~~Authors~~:	+	Structural Basis of Egg Coat-Sperm Recognition at Fertilization.,Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L Cell. 2017 Jun 15;169(7):1315-1326.e17. doi: 10.1016/j.cell.2017.05.033. PMID:28622512<ref>PMID:28622512</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5iia" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysin\|Lysin]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Al-Hosseini, H Sadat]]
		+	[[Category: Jovine, L]]
		+	[[Category: Nishimura, K]]
		+	[[Category: Raj, I]]
		+	[[Category: Sanctis, D De]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Egg coat]]
		+	[[Category: Egg coat penetration]]
		+	[[Category: Egg-sperm interaction]]
		+	[[Category: Fertilization]]
		+	[[Category: Gamete recognition]]
		+	[[Category: Sperm acrosome]]
		+	[[Category: Sperm receptor]]
		+	[[Category: Vitelline envelope]]
		+	[[Category: Zp domain]]
		+	[[Category: Zp-n domain]]

OCA at 14:27, 15 May 2016

2016-05-15T14:27:06Z

←Older revision		Revision as of 14:27, 15 May 2016
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	The entry 5iia is ON HOLD until Paper Publication		The entry 5iia is ON HOLD until Paper Publication

-	Authors: ~~Sadat Al-Hosseini, H., Raj, I., Nishimura, K., De Sanctis, D., Jovine, L.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Sadat Al-Hosseini, H]]
-	[[Category: Raj, I]]
-	[[Category: De Sanctis, D]]
-	[[Category: Nishimura, K]]
-	[[Category: Jovine, L]]

OCA at 00:31, 11 May 2016

2016-05-11T00:31:30Z

←Older revision		Revision as of 00:31, 11 May 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5iia is ON HOLD	+	The entry 5iia is ON HOLD until Paper Publication

	Authors: Sadat Al-Hosseini, H., Raj, I., Nishimura, K., De Sanctis, D., Jovine, L.		Authors: Sadat Al-Hosseini, H., Raj, I., Nishimura, K., De Sanctis, D., Jovine, L.

OCA: Protected "5iia" [edit=sysop:move=sysop]

2016-03-10T02:22:18Z

Protected "5iia" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:22, 10 March 2016

OCA: New page: '''Unreleased structure''' The entry 5iia is ON HOLD Authors: Sadat Al-Hosseini, H., Raj, I., Nishimura, K., De Sanctis, D., Jovine, L. Description: [[Category: Unreleased Structures]...

2016-03-10T02:22:17Z

New page: '''Unreleased structure''' The entry 5iia is ON HOLD Authors: Sadat Al-Hosseini, H., Raj, I., Nishimura, K., De Sanctis, D., Jovine, L. Description: [[Category: Unreleased Structures]...

New page

'''Unreleased structure'''

The entry 5iia is ON HOLD

Authors: Sadat Al-Hosseini, H., Raj, I., Nishimura, K., De Sanctis, D., Jovine, L.

Description:
[[Category: Unreleased Structures]]
[[Category: Sadat Al-Hosseini, H]]
[[Category: Raj, I]]
[[Category: De Sanctis, D]]
[[Category: Nishimura, K]]
[[Category: Jovine, L]]