5irs - Revision history

OCA at 14:07, 30 August 2023

2023-08-30T14:07:19Z

←Older revision		Revision as of 14:07, 30 August 2023
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	<StructureSection load='5irs' size='340' side='right'caption='[[5irs]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5irs' size='340' side='right'caption='[[5irs]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5irs]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IRS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5IRS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5irs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IRS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IRS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DTT:2~~,~~3-DIHYDROXY-~~1~~,4-DITHIOBUTANE'>DTT</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.796Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ADRM1, GP110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5irs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5irs OCA], [~~http~~://pdbe.org/5irs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5irs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5irs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5irs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5irs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5irs OCA], [https://pdbe.org/5irs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5irs RCSB], [https://www.ebi.ac.uk/pdbsum/5irs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5irs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ADRM1_HUMAN ADRM1_HUMAN]] Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.<ref>PMID:16990800</ref> <ref>PMID:17139257</ref> <ref>PMID:16815440</ref> <ref>PMID:16906146</ref> <ref>PMID:18497817</ref>	+	[https://www.uniprot.org/uniprot/ADRM1_HUMAN ADRM1_HUMAN] Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.<ref>PMID:16990800</ref> <ref>PMID:17139257</ref> <ref>PMID:16815440</ref> <ref>PMID:16906146</ref> <ref>PMID:18497817</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aihara, H]]	+	[[Category: Aihara H]]
-	[[Category: Chen, X]]	+	[[Category: Chen X]]
-	[[Category: Shi, K]]	+	[[Category: Shi K]]
-	[[Category: Walters, K]]	+	[[Category: Walters K]]
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Ubiquitin~~]]	+

OCA at 07:28, 3 June 2020

2020-06-03T07:28:52Z

←Older revision		Revision as of 07:28, 3 June 2020
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	==crystal structure of the proteasomal Rpn13 PRU-domain==		==crystal structure of the proteasomal Rpn13 PRU-domain==
-	<StructureSection load='5irs' size='340' side='right' caption='[[5irs]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5irs' size='340' side='right'caption='[[5irs]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5irs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IRS OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca.weizmann~~.~~ac.il/oca-docs~~/fgij/fg.htm?mol=5IRS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5irs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IRS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IRS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5irs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5irs OCA], [http://pdbe.org/5irs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5irs RCSB], [http://www.ebi.ac.uk/pdbsum/5irs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5irs ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ADRM1, GP110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5irs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5irs OCA], [http://pdbe.org/5irs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5irs RCSB], [http://www.ebi.ac.uk/pdbsum/5irs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5irs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Aihara, H]]		[[Category: Aihara, H]]
	[[Category: Chen, X]]		[[Category: Chen, X]]

OCA at 15:17, 26 July 2016

2016-07-26T15:17:00Z

←Older revision		Revision as of 15:17, 26 July 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5irs is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==crystal structure of the proteasomal Rpn13 PRU-domain==
		+	<StructureSection load='5irs' size='340' side='right' caption='[[5irs]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5irs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IRS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IRS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5irs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5irs OCA], [http://pdbe.org/5irs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5irs RCSB], [http://www.ebi.ac.uk/pdbsum/5irs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5irs ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ADRM1_HUMAN ADRM1_HUMAN]] Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.<ref>PMID:16990800</ref> <ref>PMID:17139257</ref> <ref>PMID:16815440</ref> <ref>PMID:16906146</ref> <ref>PMID:18497817</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Three receptors (Rpn1/S2/PSMD2, Rpn10/S5a, Rpn13/Adrm1) in the proteasome bind substrates by interacting with conjugated ubiquitin chains and/or shuttle factors (Rad23/HR23, Dsk2/PLIC/ubiquilin, Ddi1) that carry ubiquitinated substrates to proteasomes. We solved the structure of two such receptors with their preferred shuttle factor, namely hRpn13Pru:hPLIC2UBL and scRpn1 T1:scRad23UBL. We find that ubiquitin folds in Rad23 and Dsk2 are fine-tuned by residue substitutions to achieve high affinity for Rpn1 and Rpn13, respectively. A single substitution in hPLIC2 yields enhanced interactions with the Rpn13 ubiquitin contact surface and sterically blocks hRpn13 binding to its preferred ubiquitin chain type, K48-linked chains. Rpn1 T1 binds two ubiquitins in tandem and we find that Rad23 binds exclusively to the higher-affinity Helix28/Helix30 site. Rad23 contacts at Helix28/Helix30 are optimized compared to ubiquitin by multiple conservative amino acid substitutions. Thus, shuttle factors deliver substrates to proteasomes through fine-tuned ubiquitin-like surfaces.

-	~~Authors~~:	+	Structures of Rpn1 T1:Rad23 and hRpn13:hPLIC2 Reveal Distinct Binding Mechanisms between Substrate Receptors and Shuttle Factors of the Proteasome.,Chen X, Randles L, Shi K, Tarasov SG, Aihara H, Walters KJ Structure. 2016 Jul 6. pii: S0969-2126(16)30125-3. doi:, 10.1016/j.str.2016.05.018. PMID:27396824<ref>PMID:27396824</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5irs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aihara, H]]
		+	[[Category: Chen, X]]
		+	[[Category: Shi, K]]
		+	[[Category: Walters, K]]
		+	[[Category: Protein binding]]
		+	[[Category: Ubiquitin]]

OCA at 14:40, 15 May 2016

2016-05-15T14:40:36Z

←Older revision		Revision as of 14:40, 15 May 2016
Line 3:		Line 3:
	The entry 5irs is ON HOLD until Paper Publication		The entry 5irs is ON HOLD until Paper Publication

-	Authors: ~~Chen, X., Shi, K., Walters, K., Aihara, H.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Aihara, H]]
-	[[Category: Shi, K]]
-	[[Category: Walters, K]]
-	[[Category: Chen, X]]

OCA: Protected "5irs" [edit=sysop:move=sysop]

2016-05-11T00:50:27Z

Protected "5irs" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:50, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5irs is ON HOLD until Paper Publication Authors: Chen, X., Shi, K., Walters, K., Aihara, H. Description: Category: Unreleased Structures [[Cat...

2016-05-11T00:50:26Z

New page: '''Unreleased structure''' The entry 5irs is ON HOLD until Paper Publication Authors: Chen, X., Shi, K., Walters, K., Aihara, H. Description: Category: Unreleased Structures [[Cat...

New page

'''Unreleased structure'''

The entry 5irs is ON HOLD until Paper Publication

Authors: Chen, X., Shi, K., Walters, K., Aihara, H.

Description:
[[Category: Unreleased Structures]]
[[Category: Aihara, H]]
[[Category: Shi, K]]
[[Category: Walters, K]]
[[Category: Chen, X]]