5it1 - Revision history

OCA at 09:17, 20 March 2024

2024-03-20T09:17:02Z

←Older revision		Revision as of 09:17, 20 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q70AS3_STRPE Q70AS3_STRPE]		[https://www.uniprot.org/uniprot/Q70AS3_STRPE Q70AS3_STRPE]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Cytochrome P450 monooxygenases (CYP, EC 1.14.14.1) belong to a large family of enzymes that catalyze the hydroxylation of various substrates. Here, we present the crystal structure of CYP105P2 isolated from Streptomyces peucetius ATCC27952 at a 2.1 A resolution. The structure shows the presence of a pseudo-ligand molecule in the active site, which was co-purified fortuitously and is presumed to be a biphenyl derivative. Comparison with previously determined substrate-bound CYP structures showed that binding of the ligand produces large and distinctive conformational changes in alpha2-alpha3, alpha7-alpha9, and the C-terminal loop regions. This structural flexibility confirms our previous observation that CYP105P2 can accommodate a broad range of ligands. The structure complexed with a pseudo-ligand provides the first molecular view of CYP105P2-ligand interactions, and it indicates the involvement of hydrophobic residues (Pro82, Ala181, Met187, Leu189, Leu193, and Ile236) in the interactions between hydrophobic ligands and CYP105P2. These results provide useful insights into the structural changes involved in the recognition of different ligands by CYP105P2.
-
-	Crystal Structure of Cytochrome P450 (CYP105P2) from Streptomyces peucetius and Its Conformational Changes in Response to Substrate Binding.,Lee CW, Lee JH, Rimal H, Park H, Lee JH, Oh TJ Int J Mol Sci. 2016 May 25;17(6). pii: E813. doi: 10.3390/ijms17060813. PMID:27231902<ref>PMID:27231902</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5it1" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 14:08, 30 August 2023

2023-08-30T14:08:23Z

←Older revision		Revision as of 14:08, 30 August 2023
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	<StructureSection load='5it1' size='340' side='right'caption='[[5it1]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5it1' size='340' side='right'caption='[[5it1]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5it1]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1799 As 4.1799~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IT1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5IT1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5it1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_peucetius Streptomyces peucetius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IT1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=2OH:4,4~~-~~PROPANE-2~~,2~~-DIYLDIPHENOL'>2OH</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>cyp7863 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1950 AS 4.1799])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2OH:4,4-PROPANE-2,2-DIYLDIPHENOL'>2OH</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5it1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5it1 OCA], [https://pdbe.org/5it1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5it1 RCSB], [https://www.ebi.ac.uk/pdbsum/5it1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5it1 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5it1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5it1 OCA], [~~http~~://pdbe.org/5it1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5it1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5it1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5it1 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q70AS3_STRPE Q70AS3_STRPE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1799]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Unspecific monooxygenase~~]]	+	[[Category: Streptomyces peucetius]]
-	[[Category: Lee~~, C W~~]]	+	[[Category: Lee CW]]
-	[[Category: Lee~~, J H]]~~	+	[[Category: Lee JH]]
-	~~[[Category: Cyp105p2-biphenyl compound complex]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:30, 3 June 2020

2020-06-03T07:30:34Z

←Older revision		Revision as of 07:30, 3 June 2020
Line 1:		Line 1:

	==Streptomyces peucetius CYP105P2 complex with biphenyl compound==		==Streptomyces peucetius CYP105P2 complex with biphenyl compound==
-	<StructureSection load='5it1' size='340' side='right' caption='[[5it1]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5it1' size='340' side='right'caption='[[5it1]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5it1]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IT1 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IT1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5it1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1799 As 4.1799]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IT1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IT1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2OH:4,4-PROPANE-2,2-DIYLDIPHENOL'>2OH</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2OH:4,4-PROPANE-2,2-DIYLDIPHENOL'>2OH</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cyp7863 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1950 AS 4.1799])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5it1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5it1 OCA], [http://pdbe.org/5it1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5it1 RCSB], [http://www.ebi.ac.uk/pdbsum/5it1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5it1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5it1 OCA], [http://pdbe.org/5it1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5it1 RCSB], [http://www.ebi.ac.uk/pdbsum/5it1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5it1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 4 1799]]
		+	[[Category: Large Structures]]
	[[Category: Unspecific monooxygenase]]		[[Category: Unspecific monooxygenase]]
	[[Category: Lee, C W]]		[[Category: Lee, C W]]

OCA at 08:05, 20 June 2016

2016-06-20T08:05:05Z

←Older revision		Revision as of 08:05, 20 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5it1 is ~~ON HOLD until Paper~~ Publication	+	==Streptomyces peucetius CYP105P2 complex with biphenyl compound==
		+	<StructureSection load='5it1' size='340' side='right' caption='[[5it1]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5it1]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IT1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IT1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2OH:4,4-PROPANE-2,2-DIYLDIPHENOL'>2OH</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5it1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5it1 OCA], [http://pdbe.org/5it1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5it1 RCSB], [http://www.ebi.ac.uk/pdbsum/5it1 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytochrome P450 monooxygenases (CYP, EC 1.14.14.1) belong to a large family of enzymes that catalyze the hydroxylation of various substrates. Here, we present the crystal structure of CYP105P2 isolated from Streptomyces peucetius ATCC27952 at a 2.1 A resolution. The structure shows the presence of a pseudo-ligand molecule in the active site, which was co-purified fortuitously and is presumed to be a biphenyl derivative. Comparison with previously determined substrate-bound CYP structures showed that binding of the ligand produces large and distinctive conformational changes in alpha2-alpha3, alpha7-alpha9, and the C-terminal loop regions. This structural flexibility confirms our previous observation that CYP105P2 can accommodate a broad range of ligands. The structure complexed with a pseudo-ligand provides the first molecular view of CYP105P2-ligand interactions, and it indicates the involvement of hydrophobic residues (Pro82, Ala181, Met187, Leu189, Leu193, and Ile236) in the interactions between hydrophobic ligands and CYP105P2. These results provide useful insights into the structural changes involved in the recognition of different ligands by CYP105P2.

-	~~Authors:~~ Lee, ~~C.W.~~, Lee, J.H.	+	Crystal Structure of Cytochrome P450 (CYP105P2) from Streptomyces peucetius and Its Conformational Changes in Response to Substrate Binding.,Lee CW, Lee JH, Rimal H, Park H, Lee JH, Oh TJ Int J Mol Sci. 2016 May 25;17(6). pii: E813. doi: 10.3390/ijms17060813. PMID:27231902<ref>PMID:27231902</ref>

-	~~Description~~: ~~Streptomyces peucetius CYP105P2 complex with biprenyl compound~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Lee, C.W]]	+	<div class="pdbe-citations 5it1" style="background-color:#fffaf0;"></div>
-	[[Category: Lee, J.H]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Unspecific monooxygenase]]
		+	[[Category: Lee, C W]]
		+	[[Category: Lee, J H]]
		+	[[Category: Cyp105p2-biphenyl compound complex]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "5it1" [edit=sysop:move=sysop]

2016-05-11T00:53:04Z

Protected "5it1" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:53, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5it1 is ON HOLD until Paper Publication Authors: Lee, C.W., Lee, J.H. Description: Streptomyces peucetius CYP105P2 complex with biprenyl compound [...

2016-05-11T00:53:03Z

New page: '''Unreleased structure''' The entry 5it1 is ON HOLD until Paper Publication Authors: Lee, C.W., Lee, J.H. Description: Streptomyces peucetius CYP105P2 complex with biprenyl compound [...

New page

'''Unreleased structure'''

The entry 5it1 is ON HOLD until Paper Publication

Authors: Lee, C.W., Lee, J.H.

Description: Streptomyces peucetius CYP105P2 complex with biprenyl compound
[[Category: Unreleased Structures]]
[[Category: Lee, C.W]]
[[Category: Lee, J.H]]