5ixt - Revision history

OCA at 10:37, 6 September 2023

2023-09-06T10:37:24Z

←Older revision		Revision as of 10:37, 6 September 2023
Line 1:		Line 1:

	==The crystal structure of the Arabidopsis receptor kinase HAESA LRR ectdomain in complex with a N-terminal extended IDA peptide hormone ligand.==		==The crystal structure of the Arabidopsis receptor kinase HAESA LRR ectdomain in complex with a N-terminal extended IDA peptide hormone ligand.==
-	<StructureSection load='5ixt' size='340' side='right' caption='[[5ixt]], [[Resolution\|resolution]] 1.94Å' scene=''>	+	<StructureSection load='5ixt' size='340' side='right'caption='[[5ixt]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ixt]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IXT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5IXT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ixt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IXT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.94Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RLK5, HAE, At4g28490, F21O9.180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixt OCA], [https://pdbe.org/5ixt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ixt RCSB], [https://www.ebi.ac.uk/pdbsum/5ixt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixt ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixt OCA], [~~http~~://pdbe.org/5ixt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ixt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ixt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixt ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RLK5_ARATH RLK5_ARATH]] Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates that controls floral organ abscission. May interact with the 'INFLORESCENCE DEFICIENT IN ABSCISSION' (IDA) ligands family.<ref>PMID:10640280</ref> <ref>PMID:18660431</ref> <ref>PMID:18809915</ref> [[http://www.uniprot.org/uniprot/IDA_ARATH IDA_ARATH]] Involved in an ethylene-independent separation step of floral abscission. May act with RLK5 and HSL2 as ligand-receptor pairs.<ref>PMID:18660431</ref>	+	[https://www.uniprot.org/uniprot/RLK5_ARATH RLK5_ARATH] Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates that controls floral organ abscission. May interact with the 'INFLORESCENCE DEFICIENT IN ABSCISSION' (IDA) ligands family.<ref>PMID:10640280</ref> <ref>PMID:18660431</ref> <ref>PMID:18809915</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
-	[[Category: ~~Hothorn, M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Santiago, J~~]]	+	[[Category: Hothorn M]]
-	[[Category: ~~Membrane receptor kinase]]~~	+	[[Category: Santiago J]]
-	~~[[Category: Organ shedding]]~~	+
-	~~[[Category: Peptide hormone receptor]]~~	+
-	~~[[Category: Plant development]]~~	+
-	~~[[Category: Signaling complex]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 12:49, 16 November 2017

2017-11-16T12:49:53Z

←Older revision		Revision as of 12:49, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='5ixt' size='340' side='right' caption='[[5ixt]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='5ixt' size='340' side='right' caption='[[5ixt]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ixt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IXT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ixt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IXT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RLK5, HAE, At4g28490, F21O9.180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixt OCA], [http://pdbe.org/5ixt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ixt RCSB], [http://www.ebi.ac.uk/pdbsum/5ixt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixt OCA], [http://pdbe.org/5ixt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ixt RCSB], [http://www.ebi.ac.uk/pdbsum/5ixt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixt ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Hothorn, M]]		[[Category: Hothorn, M]]
	[[Category: Santiago, J]]		[[Category: Santiago, J]]

OCA at 09:17, 3 October 2016

2016-10-03T09:17:47Z

←Older revision		Revision as of 09:17, 3 October 2016
Line 6:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixt OCA], [http://pdbe.org/5ixt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ixt RCSB], [http://www.ebi.ac.uk/pdbsum/5ixt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixt OCA], [http://pdbe.org/5ixt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ixt RCSB], [http://www.ebi.ac.uk/pdbsum/5ixt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ixt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA: Protected "5ixt" [edit=sysop:move=sysop]

2016-05-12T18:51:43Z

Protected "5ixt" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:51, 12 May 2016

OCA: New page: ==The crystal structure of the Arabidopsis receptor kinase HAESA LRR ectdomain in complex with a N-terminal extended IDA peptide hormone ligand.==
2016-05-12T18:51:38Z

New page: ==The crystal structure of the Arabidopsis receptor kinase HAESA LRR ectdomain in complex with a N-terminal extended IDA peptide hormone ligand.== <StructureSection load='5ixt' size='340'...

New page

==The crystal structure of the Arabidopsis receptor kinase HAESA LRR ectdomain in complex with a N-terminal extended IDA peptide hormone ligand.==
<StructureSection load='5ixt' size='340' side='right' caption='[[5ixt]], [[Resolution|resolution]] 1.94Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ixt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IXT FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ixt OCA], [http://pdbe.org/5ixt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ixt RCSB], [http://www.ebi.ac.uk/pdbsum/5ixt PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/RLK5_ARATH RLK5_ARATH]] Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates that controls floral organ abscission. May interact with the 'INFLORESCENCE DEFICIENT IN ABSCISSION' (IDA) ligands family.<ref>PMID:10640280</ref> <ref>PMID:18660431</ref> <ref>PMID:18809915</ref> [[http://www.uniprot.org/uniprot/IDA_ARATH IDA_ARATH]] Involved in an ethylene-independent separation step of floral abscission. May act with RLK5 and HSL2 as ligand-receptor pairs.<ref>PMID:18660431</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Plants constantly renew during their life cycle and thus require to shed senescent and damaged organs. Floral abscission is controlled by the leucine-rich repeat receptor kinase (LRR-RK) HAESA and the peptide hormone IDA. It is unknown how expression of IDA in the abscission zone leads to HAESA activation. Here we show that IDA is sensed directly by the HAESA ectodomain. Crystal structures of HAESA in complex with IDA reveal a hormone binding pocket that accommodates an active dodecamer peptide. A central hydroxyproline residue anchors IDA to the receptor. The HAESA co-receptor SERK1, a positive regulator of the floral abscission pathway, allows for high-affinity sensing of the peptide hormone by binding to an Arg-His-Asn motif in IDA. This sequence pattern is conserved among diverse plant peptides, suggesting that plant peptide hormone receptors may share a common ligand binding mode and activation mechanism.

Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission.,Santiago J, Brandt B, Wildhagen M, Hohmann U, Hothorn LA, Butenko MA, Hothorn M Elife. 2016 Apr 8;5. pii: e15075. doi: 10.7554/eLife.15075. PMID:27058169<ref>PMID:27058169</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ixt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Hothorn, M]]
[[Category: Santiago, J]]
[[Category: Membrane receptor kinase]]
[[Category: Organ shedding]]
[[Category: Peptide hormone receptor]]
[[Category: Plant development]]
[[Category: Signaling complex]]
[[Category: Signaling protein]]