5j11 - Revision history

OCA at 10:41, 6 September 2023

2023-09-06T10:41:43Z

←Older revision		Revision as of 10:41, 6 September 2023
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	==Structure of human TSLP in complex with TSLPR and IL-7Ralpha==		==Structure of human TSLP in complex with TSLPR and IL-7Ralpha==
-	<StructureSection load='5j11' size='340' side='right' caption='[[5j11]], [[Resolution\|resolution]] 2.56Å' scene=''>	+	<StructureSection load='5j11' size='340' side='right'caption='[[5j11]], [[Resolution\|resolution]] 2.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j11]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J11 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5J11 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j11]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J11 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J11 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=NAG:N~~-~~ACETYL-D-GLUCOSAMINE'>NAG</scene>~~, ~~<scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.56Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TSLP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN]), IL7R ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN]), CRLF2, CRL2, ILXR, TSLPR ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5j11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j11 OCA], [~~http~~://pdbe.org/5j11 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5j11 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5j11 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5j11 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j11 OCA], [https://pdbe.org/5j11 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j11 RCSB], [https://www.ebi.ac.uk/pdbsum/5j11 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j11 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TSLP_HUMAN TSLP_HUMAN]] Isoform 1: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.<ref>PMID:11418668</ref> <ref>PMID:11480573</ref> <ref>PMID:17242164</ref> Isoform 2: May act as an antimicrobial peptide in the oral cavity and on the skin.<ref>PMID:24850429</ref> [[http://www.uniprot.org/uniprot/CRLF2_HUMAN CRLF2_HUMAN]] Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system.<ref>PMID:11418668</ref>	+	[https://www.uniprot.org/uniprot/TSLP_HUMAN TSLP_HUMAN] Isoform 1: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.<ref>PMID:11418668</ref> <ref>PMID:11480573</ref> <ref>PMID:17242164</ref> Isoform 2: May act as an antimicrobial peptide in the oral cavity and on the skin.<ref>PMID:24850429</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5j11" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5j11" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Interleukin receptor 3D structures\|Interleukin receptor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Savvides, S N~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Verstraete, K~~]]	+	[[Category: Savvides SN]]
-	[[Category: ~~Cytokine inflammation tslp signaling complex]]~~	+	[[Category: Verstraete K]]
-	~~[[Category: Signaling protein~~]]	+

OCA at 07:05, 28 February 2018

2018-02-28T07:05:57Z

←Older revision		Revision as of 07:05, 28 February 2018
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	<StructureSection load='5j11' size='340' side='right' caption='[[5j11]], [[Resolution\|resolution]] 2.56Å' scene=''>		<StructureSection load='5j11' size='340' side='right' caption='[[5j11]], [[Resolution\|resolution]] 2.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j11]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J11 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j11]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J11 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TSLP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), IL7R ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CRLF2, CRL2, ILXR, TSLPR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j11 OCA], [http://pdbe.org/5j11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j11 RCSB], [http://www.ebi.ac.uk/pdbsum/5j11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j11 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j11 OCA], [http://pdbe.org/5j11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j11 RCSB], [http://www.ebi.ac.uk/pdbsum/5j11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j11 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Savvides, S N]]		[[Category: Savvides, S N]]
	[[Category: Verstraete, K]]		[[Category: Verstraete, K]]
	[[Category: Cytokine inflammation tslp signaling complex]]		[[Category: Cytokine inflammation tslp signaling complex]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 06:11, 19 April 2017

2017-04-19T06:11:52Z

←Older revision		Revision as of 06:11, 19 April 2017
Line 1:		Line 1:

-	==~~Cytokine receptor~~ complex==	+	==Structure of human TSLP in complex with TSLPR and IL-7Ralpha==
	<StructureSection load='5j11' size='340' side='right' caption='[[5j11]], [[Resolution\|resolution]] 2.56Å' scene=''>		<StructureSection load='5j11' size='340' side='right' caption='[[5j11]], [[Resolution\|resolution]] 2.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/TSLP_HUMAN TSLP_HUMAN]] Isoform 1: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.<ref>PMID:11418668</ref> <ref>PMID:11480573</ref> <ref>PMID:17242164</ref> Isoform 2: May act as an antimicrobial peptide in the oral cavity and on the skin.<ref>PMID:24850429</ref> [[http://www.uniprot.org/uniprot/CRLF2_HUMAN CRLF2_HUMAN]] Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system.<ref>PMID:11418668</ref>		[[http://www.uniprot.org/uniprot/TSLP_HUMAN TSLP_HUMAN]] Isoform 1: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.<ref>PMID:11418668</ref> <ref>PMID:11480573</ref> <ref>PMID:17242164</ref> Isoform 2: May act as an antimicrobial peptide in the oral cavity and on the skin.<ref>PMID:24850429</ref> [[http://www.uniprot.org/uniprot/CRLF2_HUMAN CRLF2_HUMAN]] Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system.<ref>PMID:11418668</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The pro-inflammatory cytokine thymic stromal lymphopoietin (TSLP) is pivotal to the pathophysiology of widespread allergic diseases mediated by type 2 helper T cell (Th2) responses, including asthma and atopic dermatitis. The emergence of human TSLP as a clinical target against asthma calls for maximally harnessing its therapeutic potential via structural and mechanistic considerations. Here we employ an integrative experimental approach focusing on productive and antagonized TSLP complexes and free cytokine. We reveal how cognate receptor TSLPR allosterically activates TSLP to potentiate the recruitment of the shared interleukin 7 receptor alpha-chain (IL-7Ralpha) by leveraging the flexibility, conformational heterogeneity and electrostatics of the cytokine. We further show that the monoclonal antibody Tezepelumab partly exploits these principles to neutralize TSLP activity. Finally, we introduce a fusion protein comprising a tandem of the TSLPR and IL-7Ralpha extracellular domains, which harnesses the mechanistic intricacies of the TSLP-driven receptor complex to manifest high antagonistic potency.
		+
		+	Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma.,Verstraete K, Peelman F, Braun H, Lopez J, Van Rompaey D, Dansercoer A, Vandenberghe I, Pauwels K, Tavernier J, Lambrecht BN, Hammad H, De Winter H, Beyaert R, Lippens G, Savvides SN Nat Commun. 2017 Apr 3;8:14937. doi: 10.1038/ncomms14937. PMID:28368013<ref>PMID:28368013</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5j11" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:25, 5 April 2017

2017-04-05T13:25:56Z

←Older revision		Revision as of 13:25, 5 April 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5j11 is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Cytokine receptor complex==
-		+	<StructureSection load='5j11' size='340' side='right' caption='[[5j11]], [[Resolution\|resolution]] 2.56Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5j11]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J11 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j11 OCA], [http://pdbe.org/5j11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j11 RCSB], [http://www.ebi.ac.uk/pdbsum/5j11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j11 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TSLP_HUMAN TSLP_HUMAN]] Isoform 1: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.<ref>PMID:11418668</ref> <ref>PMID:11480573</ref> <ref>PMID:17242164</ref> Isoform 2: May act as an antimicrobial peptide in the oral cavity and on the skin.<ref>PMID:24850429</ref> [[http://www.uniprot.org/uniprot/CRLF2_HUMAN CRLF2_HUMAN]] Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system.<ref>PMID:11418668</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Savvides, S N]]
		+	[[Category: Verstraete, K]]
		+	[[Category: Cytokine inflammation tslp signaling complex]]
		+	[[Category: Signaling protein]]

OCA at 14:57, 15 May 2016

2016-05-15T14:57:11Z

←Older revision		Revision as of 14:57, 15 May 2016
Line 3:		Line 3:
	The entry 5j11 is ON HOLD until Paper Publication		The entry 5j11 is ON HOLD until Paper Publication

-	Authors: ~~Verstraete, K., Savvides, S.N.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Savvides, S.N]]
-	[[Category: Verstraete, K]]

OCA: Protected "5j11" [edit=sysop:move=sysop]

2016-05-11T01:17:01Z

Protected "5j11" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:17, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5j11 is ON HOLD until Paper Publication Authors: Verstraete, K., Savvides, S.N. Description: Category: Unreleased Structures [[Category: Savvi...

2016-05-11T01:16:59Z

New page: '''Unreleased structure''' The entry 5j11 is ON HOLD until Paper Publication Authors: Verstraete, K., Savvides, S.N. Description: Category: Unreleased Structures [[Category: Savvi...

New page

'''Unreleased structure'''

The entry 5j11 is ON HOLD until Paper Publication

Authors: Verstraete, K., Savvides, S.N.

Description:
[[Category: Unreleased Structures]]
[[Category: Savvides, S.N]]
[[Category: Verstraete, K]]