5j2u - Revision history

OCA at 10:43, 6 September 2023

2023-09-06T10:43:25Z

←Older revision		Revision as of 10:43, 6 September 2023
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	<StructureSection load='5j2u' size='340' side='right'caption='[[5j2u]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='5j2u' size='340' side='right'caption='[[5j2u]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j2u]] is a 6 chain structure with sequence from [~~http://en.wikipedia.org/wiki/Bovin Bovin], [http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [~~http~~://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J2U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5J2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j2u]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J2U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6DO:(2~{S})-2-[[(2~{R},3~{R})-3-METHOXY-3-[(2~{S})-1-[(3~{R},4~{S},5~{S})-3-METHOXY-5-METHYL-4-[METHYL-[(2~{S})-3-METHYL-2-[[(2~{S})-3-METHYL-2-(METHYLAMINO)BUTANOYL]AMINO]BUTANOYL]AMINO]HEPTANOYL]PYRROLIDIN-2-YL]-2-METHYL-PROPANOYL]AMINO]-3-PHENYL-PROPANOIC+ACID'>6DO</scene>, <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5iyz\|5iyz]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6DO:(2~{S})-2-[[(2~{R},3~{R})-3-METHOXY-3-[(2~{S})-1-[(3~{R},4~{S},5~{S})-3-METHOXY-5-METHYL-4-[METHYL-[(2~{S})-3-METHYL-2-[[(2~{S})-3-METHYL-2-(METHYLAMINO)BUTANOYL]AMINO]BUTANOYL]AMINO]HEPTANOYL]PYRROLIDIN-2-YL]-2-METHYL-PROPANOYL]AMINO]-3-PHENYL-PROPANOIC+ACID'>6DO</scene>, <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Stmn4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), TUBB2B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j2u OCA], [https://pdbe.org/5j2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j2u RCSB], [https://www.ebi.ac.uk/pdbsum/5j2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j2u ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j2u OCA], [~~http~~://pdbe.org/5j2u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5j2u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5j2u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5j2u ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). [[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. ~~[[http://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT]] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref>~~	+	[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 24:
	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]		*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
	*[[Tubulin tyrosine ligase\|Tubulin tyrosine ligase]]		*[[Tubulin tyrosine ligase\|Tubulin tyrosine ligase]]
-	*[[User:Alisa Cario\|User:Alisa Cario]]
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
-	[[Category: Bovin]]
-	[[Category: Buffalo rat]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bargsten, K]]	+	[[Category: Rattus norvegicus]]
-	[[Category: Doronina, S]]	+	[[Category: Bargsten K]]
-	[[Category: Prota~~, A E~~]]	+	[[Category: Doronina S]]
-	[[Category: Steinmetz~~, M O~~]]	+	[[Category: Prota AE]]
-	[[Category: Sussman, D]]	+	[[Category: Steinmetz MO]]
-	[[Category: Waight~~, A B]]~~	+	[[Category: Sussman D]]
-	~~[[Category: Cell cycle]]~~	+	[[Category: Waight AB]]
-	~~[[Category: Cytoskeleton]]~~	+
-	~~[[Category: Microtubule]]~~	+
-	~~[[Category: Tubulin fold~~]]	+

OCA at 07:26, 10 June 2020

2020-06-10T07:26:43Z

←Older revision		Revision as of 07:26, 10 June 2020
Line 1:		Line 1:

	==Tubulin-MMAF complex==		==Tubulin-MMAF complex==
-	<StructureSection load='5j2u' size='340' side='right' caption='[[5j2u]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='5j2u' size='340' side='right'caption='[[5j2u]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5j2u]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] ~~and~~ [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J2U OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5J2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5j2u]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin], [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J2U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5J2U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6DO:(2~{S})-2-[[(2~{R},3~{R})-3-METHOXY-3-[(2~{S})-1-[(3~{R},4~{S},5~{S})-3-METHOXY-5-METHYL-4-[METHYL-[(2~{S})-3-METHYL-2-[[(2~{S})-3-METHYL-2-(METHYLAMINO)BUTANOYL]AMINO]BUTANOYL]AMINO]HEPTANOYL]PYRROLIDIN-2-YL]-2-METHYL-PROPANOYL]AMINO]-3-PHENYL-PROPANOIC+ACID'>6DO</scene>, <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6DO:(2~{S})-2-[[(2~{R},3~{R})-3-METHOXY-3-[(2~{S})-1-[(3~{R},4~{S},5~{S})-3-METHOXY-5-METHYL-4-[METHYL-[(2~{S})-3-METHYL-2-[[(2~{S})-3-METHYL-2-(METHYLAMINO)BUTANOYL]AMINO]BUTANOYL]AMINO]HEPTANOYL]PYRROLIDIN-2-YL]-2-METHYL-PROPANOYL]AMINO]-3-PHENYL-PROPANOIC+ACID'>6DO</scene>, <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5iyz\|5iyz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5iyz\|5iyz]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j2u OCA], [http://pdbe.org/5j2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j2u RCSB], [http://www.ebi.ac.uk/pdbsum/5j2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j2u ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Stmn4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), TUBB2B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j2u OCA], [http://pdbe.org/5j2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j2u RCSB], [http://www.ebi.ac.uk/pdbsum/5j2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j2u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:

	==See Also==		==See Also==
-	*[[Stathmin\|Stathmin]]	+	*[[Stathmin-4 3D structures\|Stathmin-4 3D structures]]
-	*[[Tubulin\|Tubulin]]	+	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
	*[[Tubulin tyrosine ligase\|Tubulin tyrosine ligase]]		*[[Tubulin tyrosine ligase\|Tubulin tyrosine ligase]]
		+	*[[User:Alisa Cario\|User:Alisa Cario]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Bovin]]
		+	[[Category: Buffalo rat]]
		+	[[Category: Large Structures]]
	[[Category: Bargsten, K]]		[[Category: Bargsten, K]]
	[[Category: Doronina, S]]		[[Category: Doronina, S]]

OCA at 09:28, 3 October 2016

2016-10-03T09:28:40Z

←Older revision		Revision as of 09:28, 3 October 2016
Line 22:		Line 22:
	==See Also==		==See Also==
	*[[Stathmin\|Stathmin]]		*[[Stathmin\|Stathmin]]
		+	*[[Tubulin\|Tubulin]]
		+	*[[Tubulin tyrosine ligase\|Tubulin tyrosine ligase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:01, 10 September 2016

2016-09-10T07:01:14Z

←Older revision		Revision as of 07:01, 10 September 2016
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5j2u" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5j2u" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Stathmin\|Stathmin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:05, 10 September 2016

2016-09-10T00:05:07Z

←Older revision		Revision as of 00:05, 10 September 2016
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5j2u]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J2U FirstGlance]. <br>		<table><tr><td colspan='2'>[[5j2u]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J2U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6DO:(2~{S})-2-[[(2~{R},3~{R})-3-~~methoxy~~-3-[(2~{S})-1-[(3~{R},4~{S},5~{S})-3-~~methoxy~~-5-~~methyl~~-4-[~~methyl~~-[(2~{S})-3-~~methyl~~-2-[[(2~{S})-3-~~methyl~~-2-(~~methylamino~~)~~butanoyl~~]~~amino~~]~~butanoyl~~]~~amino~~]~~heptanoyl~~]~~pyrrolidin~~-2-yl]-2-~~methyl~~-~~propanoyl~~]~~amino~~]-3-~~phenyl~~-~~propanoic~~+~~acid~~'>6DO</scene>, <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6DO:(2~{S})-2-[[(2~{R},3~{R})-3-METHOXY-3-[(2~{S})-1-[(3~{R},4~{S},5~{S})-3-METHOXY-5-METHYL-4-[METHYL-[(2~{S})-3-METHYL-2-[[(2~{S})-3-METHYL-2-(METHYLAMINO)BUTANOYL]AMINO]BUTANOYL]AMINO]HEPTANOYL]PYRROLIDIN-2-YL]-2-METHYL-PROPANOYL]AMINO]-3-PHENYL-PROPANOIC+ACID'>6DO</scene>, <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5iyz\|5iyz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5iyz\|5iyz]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j2u OCA], [http://pdbe.org/5j2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j2u RCSB], [http://www.ebi.ac.uk/pdbsum/5j2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j2u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j2u OCA], [http://pdbe.org/5j2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j2u RCSB], [http://www.ebi.ac.uk/pdbsum/5j2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j2u ProSAT]</span></td></tr>

OCA at 05:37, 9 September 2016

2016-09-09T05:37:23Z

←Older revision		Revision as of 05:37, 9 September 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5j2u is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Tubulin-MMAF complex==
		+	<StructureSection load='5j2u' size='340' side='right' caption='[[5j2u]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5j2u]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J2U FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6DO:(2~{S})-2-[[(2~{R},3~{R})-3-methoxy-3-[(2~{S})-1-[(3~{R},4~{S},5~{S})-3-methoxy-5-methyl-4-[methyl-[(2~{S})-3-methyl-2-[[(2~{S})-3-methyl-2-(methylamino)butanoyl]amino]butanoyl]amino]heptanoyl]pyrrolidin-2-yl]-2-methyl-propanoyl]amino]-3-phenyl-propanoic+acid'>6DO</scene>, <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5iyz\|5iyz]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j2u OCA], [http://pdbe.org/5j2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j2u RCSB], [http://www.ebi.ac.uk/pdbsum/5j2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j2u ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). [[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT]] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The auristatin class of microtubule destabilizers are highly potent cytotoxic agents against several cancer cell types when delivered as antibody drug conjugates. Here we describe the high resolution structures of tubulin in complex with both monomethyl auristatin E and F and unambiguously define the trans-configuration of both ligands at the Val-Dil amide bond in their tubulin bound state. Moreover, we illustrate how peptidic vinca-site agents carrying terminal carboxylate residues may exploit an observed extended hydrogen bond network with the M-loop Arg278 to greatly improve the affinity of the corresponding analogs and to maintain the M-loop in an incompatible conformation for productive lateral tubulin-tubulin contacts in microtubules. Our results highlight a potential, previously undescribed molecular mechanism by which peptidic vinca-site agents maintain unparalleled potency as microtubule-destabilizing agents.

-	~~Authors:~~ Waight~~, A.B.~~, Bargsten, K., Doronina, S., Steinmetz~~, M.O.~~, Sussman, D., Prota, ~~A.E~~.	+	Structural Basis of Microtubule Destabilization by Potent Auristatin Anti-Mitotics.,Waight AB, Bargsten K, Doronina S, Steinmetz MO, Sussman D, Prota AE PLoS One. 2016 Aug 12;11(8):e0160890. doi: 10.1371/journal.pone.0160890., eCollection 2016. PMID:27518442<ref>PMID:27518442</ref>

-	~~Description: Tubulin-MMAF complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Prota, A.E~~]]	+	<div class="pdbe-citations 5j2u" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bos taurus]]
	[[Category: Bargsten, K]]		[[Category: Bargsten, K]]
	[[Category: Doronina, S]]		[[Category: Doronina, S]]
-	[[Category: Steinmetz, M.O]]	+	[[Category: Prota, A E]]
		+	[[Category: Steinmetz, M O]]
	[[Category: Sussman, D]]		[[Category: Sussman, D]]
-	[[Category: Waight, A.B]]	+	[[Category: Waight, A B]]
		+	[[Category: Cell cycle]]
		+	[[Category: Cytoskeleton]]
		+	[[Category: Microtubule]]
		+	[[Category: Tubulin fold]]

OCA at 12:17, 10 August 2016

2016-08-10T12:17:42Z

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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5j2u is ON HOLD	+	The entry 5j2u is ON HOLD until Paper Publication

	Authors: Waight, A.B., Bargsten, K., Doronina, S., Steinmetz, M.O., Sussman, D., Prota, A.E.		Authors: Waight, A.B., Bargsten, K., Doronina, S., Steinmetz, M.O., Sussman, D., Prota, A.E.

OCA: Protected "5j2u" [edit=sysop:move=sysop]

2016-05-11T01:21:55Z

Protected "5j2u" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:21, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5j2u is ON HOLD Authors: Waight, A.B., Bargsten, K., Doronina, S., Steinmetz, M.O., Sussman, D., Prota, A.E. Description: Tubulin-MMAF complex [[Ca...

2016-05-11T01:21:54Z

New page: '''Unreleased structure''' The entry 5j2u is ON HOLD Authors: Waight, A.B., Bargsten, K., Doronina, S., Steinmetz, M.O., Sussman, D., Prota, A.E. Description: Tubulin-MMAF complex [[Ca...

New page

'''Unreleased structure'''

The entry 5j2u is ON HOLD

Authors: Waight, A.B., Bargsten, K., Doronina, S., Steinmetz, M.O., Sussman, D., Prota, A.E.

Description: Tubulin-MMAF complex
[[Category: Unreleased Structures]]
[[Category: Prota, A.E]]
[[Category: Bargsten, K]]
[[Category: Doronina, S]]
[[Category: Steinmetz, M.O]]
[[Category: Sussman, D]]
[[Category: Waight, A.B]]