5ji3 - Revision history

OCA at 18:52, 20 September 2023

2023-09-20T18:52:52Z

←Older revision		Revision as of 18:52, 20 September 2023
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	<StructureSection load='5ji3' size='340' side='right'caption='[[5ji3]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='5ji3' size='340' side='right'caption='[[5ji3]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ji3]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JI3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JI3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ji3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JI3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=DAT:2-DEOXYADENOSINE-5-DIPHOSPHATE'>DAT</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Related_structure~~\|~~Related:]]</b></td><td class="sblockDat">[[1g4a\|1g4a~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAT:2-DEOXYADENOSINE-5-DIPHOSPHATE'>DAT</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>hslV, EC55989_4410 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895]), hslU, htpI, Z5478, ECs4858 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ji3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ji3 OCA], [https://pdbe.org/5ji3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ji3 RCSB], [https://www.ebi.ac.uk/pdbsum/5ji3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ji3 ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/HslU~~--~~HslV_peptidase HslU~~-~~-HslV peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.2 3.4.25.2]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ji3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ji3 OCA], [~~http~~://pdbe.org/5ji3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ji3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ji3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ji3 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~HSLV_ECO55 HSLV_ECO55]~~] Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery. ~~[[http://www.uniprot.org/uniprot/HSLU_ECO57 HSLU_ECO57]] ATPase subunit of a proteasome-like degradation~~ complex~~; this subunit~~ has ~~chaperone activity. The binding~~ of ~~ATP~~ and ~~its subsequent hydrolysis by HslU~~ are ~~essential for unfolding of protein substrates subsequently~~ hydrolyzed by HslV. ~~HslU recognizes~~ the N-~~terminal part~~ of ~~its~~ protein substrates and unfolds these before ~~they are guided~~ to HslV for hydrolysis.	+	[https://www.uniprot.org/uniprot/HSLV_ECOLI HSLV_ECOLI] Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery. The complex has been shown to be involved in the specific degradation of heat shock induced transcription factors such as RpoH and SulA. In addition, small hydrophobic peptides are also hydrolyzed by HslV. HslV has weak protease activity even in the absence of HslU, but this activity is induced more than 100-fold in the presence of HslU. HslU recognizes protein substrates and unfolds these before guiding them to HslV for hydrolysis. HslV is not believed to degrade folded proteins.<ref>PMID:8662828</ref> <ref>PMID:8650174</ref> <ref>PMID:9288941</ref> <ref>PMID:9393683</ref> <ref>PMID:10452560</ref> <ref>PMID:10419524</ref> <ref>PMID:15696175</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: HslU--HslV peptidase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baytshtok, V]]	+	[[Category: Baytshtok V]]
-	[[Category: Grant~~, R A~~]]	+	[[Category: Grant RA]]
-	[[Category: Sauer~~, R T~~]]	+	[[Category: Sauer RT]]
-	[[Category: Schmitz~~, K R]]~~	+	[[Category: Schmitz KR]]
-	~~[[Category: Aaa+ atpase]]~~	+
-	~~[[Category: Hslvu]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase complex]]~~	+
-	~~[[Category: Peptidase]]~~	+
-	~~[[Category: Peptidase-atpase complex~~]]	+

OCA at 06:25, 16 October 2019

2019-10-16T06:25:02Z

←Older revision		Revision as of 06:25, 16 October 2019
Line 1:		Line 1:

	==HslUV complex==		==HslUV complex==
-	<StructureSection load='5ji3' size='340' side='right' caption='[[5ji3]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='5ji3' size='340' side='right'caption='[[5ji3]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ji3]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JI3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JI3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ji3]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JI3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JI3 FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 5ji3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5ji3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ATPase 3D structures\|ATPase 3D structures]]
		+	*[[Heat Shock Protein structures\|Heat Shock Protein structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 31:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: HslU--HslV peptidase]]		[[Category: HslU--HslV peptidase]]
		+	[[Category: Large Structures]]
	[[Category: Baytshtok, V]]		[[Category: Baytshtok, V]]
	[[Category: Grant, R A]]		[[Category: Grant, R A]]

OCA at 06:54, 31 January 2018

2018-01-31T06:54:25Z

←Older revision		Revision as of 06:54, 31 January 2018
Line 3:		Line 3:
	<StructureSection load='5ji3' size='340' side='right' caption='[[5ji3]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='5ji3' size='340' side='right' caption='[[5ji3]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ji3]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JI3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JI3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ji3]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JI3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JI3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAT:2-DEOXYADENOSINE-5-DIPHOSPHATE'>DAT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAT:2-DEOXYADENOSINE-5-DIPHOSPHATE'>DAT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g4a\|1g4a]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g4a\|1g4a]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hslV, EC55989_4410 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), hslU, htpI, Z5478, ECs4858 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/HslU--HslV_peptidase HslU--HslV peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.2 3.4.25.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/HslU--HslV_peptidase HslU--HslV peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.2 3.4.25.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ji3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ji3 OCA], [http://pdbe.org/5ji3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ji3 RCSB], [http://www.ebi.ac.uk/pdbsum/5ji3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ji3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ji3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ji3 OCA], [http://pdbe.org/5ji3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ji3 RCSB], [http://www.ebi.ac.uk/pdbsum/5ji3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ji3 ProSAT]</span></td></tr>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: HslU--HslV peptidase]]		[[Category: HslU--HslV peptidase]]
	[[Category: Baytshtok, V]]		[[Category: Baytshtok, V]]

OCA at 19:33, 9 December 2016

2016-12-09T19:33:38Z

←Older revision		Revision as of 19:33, 9 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The entry ~~5ji3~~ is ~~ON HOLD~~	+	==HslUV complex==
		+	<StructureSection load='5ji3' size='340' side='right' caption='[[5ji3]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ji3]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JI3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JI3 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAT:2-DEOXYADENOSINE-5-DIPHOSPHATE'>DAT</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g4a\|1g4a]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/HslU--HslV_peptidase HslU--HslV peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.2 3.4.25.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ji3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ji3 OCA], [http://pdbe.org/5ji3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ji3 RCSB], [http://www.ebi.ac.uk/pdbsum/5ji3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ji3 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HSLV_ECO55 HSLV_ECO55]] Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery. [[http://www.uniprot.org/uniprot/HSLU_ECO57 HSLU_ECO57]] ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The I domain of HslU sits above the AAA+ ring and forms a funnel-like entry to the axial pore, where protein substrates are engaged, unfolded, and translocated into HslV for degradation. The L199Q I-domain substitution, which was originally reported as a loss-of-function mutation, resides in a segment that appears to adopt multiple conformations as electron density is not observed in HslU and HslUV crystal structures. The L199Q sequence change does not alter the structure of the AAA+ ring or its interactions with HslV but increases I-domain susceptibility to limited endoproteolysis. Notably, the L199Q mutation increases the rate of ATP hydrolysis substantially, results in slower degradation of some proteins but faster degradation of other substrates, and markedly changes the preference of HslUV for initiating degradation at the N or C terminus of model substrates. Thus, a structurally dynamic region of the I domain plays a key role in controlling protein degradation by HslUV.

-	~~Authors: Grant, R.~~A., ~~Sauer~~, ~~R.T.~~, ~~Schmitz~~, K.R., ~~Baytshtok, V~~.	+	A Structurally Dynamic Region of the HslU Intermediate Domain Controls Protein Degradation and ATP Hydrolysis.,Baytshtok V, Fei X, Grant RA, Baker TA, Sauer RT Structure. 2016 Oct 4;24(10):1766-1777. doi: 10.1016/j.str.2016.08.012. Epub 2016, Sep 22. PMID:27667691<ref>PMID:27667691</ref>

-	~~Description: HslUV complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category: Schmitz~~, K.~~R]]~~	+	<div class="pdbe-citations 5ji3" style="background-color:#fffaf0;"></div>
-	~~[[Category~~: ~~Sauer, R.T]]~~	+	== References ==
-	[[Category: ~~Grant, R.A~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: HslU--HslV peptidase]]
	[[Category: Baytshtok, V]]		[[Category: Baytshtok, V]]
		+	[[Category: Grant, R A]]
		+	[[Category: Sauer, R T]]
		+	[[Category: Schmitz, K R]]
		+	[[Category: Aaa+ atpase]]
		+	[[Category: Hslvu]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase complex]]
		+	[[Category: Peptidase]]
		+	[[Category: Peptidase-atpase complex]]

OCA: Protected "5ji3" [edit=sysop:move=sysop]

2016-05-11T02:06:16Z

Protected "5ji3" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:06, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5ji3 is ON HOLD Authors: Grant, R.A., Sauer, R.T., Schmitz, K.R., Baytshtok, V. Description: HslUV complex Category: Unreleased Structures [[Ca...

2016-05-11T02:06:15Z

New page: '''Unreleased structure''' The entry 5ji3 is ON HOLD Authors: Grant, R.A., Sauer, R.T., Schmitz, K.R., Baytshtok, V. Description: HslUV complex Category: Unreleased Structures [[Ca...

New page

'''Unreleased structure'''

The entry 5ji3 is ON HOLD

Authors: Grant, R.A., Sauer, R.T., Schmitz, K.R., Baytshtok, V.

Description: HslUV complex
[[Category: Unreleased Structures]]
[[Category: Schmitz, K.R]]
[[Category: Sauer, R.T]]
[[Category: Grant, R.A]]
[[Category: Baytshtok, V]]