5jk9 - Revision history

OCA at 18:56, 20 September 2023

2023-09-20T18:56:52Z

←Older revision		Revision as of 18:56, 20 September 2023
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	<StructureSection load='5jk9' size='340' side='right'caption='[[5jk9]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5jk9' size='340' side='right'caption='[[5jk9]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jk9]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JK9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JK9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jk9]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JK9 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jke\|5jke]]~~, [[~~5jkd~~\|~~5jkd]], [[5jkc\|5jkc]], [[5jkb\|5jkb]], [[5jka\|5jka~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~IZUMO1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jk9 OCA], [https://pdbe.org/5jk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jk9 RCSB], [https://www.ebi.ac.uk/pdbsum/5jk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jk9 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jk9 OCA], [~~http~~://pdbe.org/5jk9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jk9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jk9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jk9 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/IZUM1_HUMAN IZUM1_HUMAN]] Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.<ref>PMID:15759005</ref>	+	[https://www.uniprot.org/uniprot/IZUM1_HUMAN IZUM1_HUMAN] Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.<ref>PMID:15759005</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ishida, H]]	+	[[Category: Ishida H]]
-	[[Category: Ohto, U]]	+	[[Category: Ohto U]]
-	[[Category: Shimizu, T]]	+	[[Category: Shimizu T]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Fertilization]]~~	+
-	~~[[Category: Izumo1]]~~	+
-	~~[[Category: Juno~~]]	+

OCA at 07:18, 19 February 2020

2020-02-19T07:18:50Z

←Older revision		Revision as of 07:18, 19 February 2020
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	==Crystal structure of human IZUMO1==		==Crystal structure of human IZUMO1==
-	<StructureSection load='5jk9' size='340' side='right' caption='[[5jk9]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5jk9' size='340' side='right'caption='[[5jk9]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jk9]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JK9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jk9]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JK9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jke\|5jke]], [[5jkd\|5jkd]], [[5jkc\|5jkc]], [[5jkb\|5jkb]], [[5jka\|5jka]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jke\|5jke]], [[5jkd\|5jkd]], [[5jkc\|5jkc]], [[5jkb\|5jkb]], [[5jka\|5jka]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IZUMO1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jk9 OCA], [http://pdbe.org/5jk9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jk9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jk9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jk9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jk9 OCA], [http://pdbe.org/5jk9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jk9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jk9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jk9 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Ishida, H]]		[[Category: Ishida, H]]
	[[Category: Ohto, U]]		[[Category: Ohto, U]]

OCA at 18:11, 12 July 2016

2016-07-12T18:11:48Z

←Older revision		Revision as of 18:11, 12 July 2016
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jke\|5jke]], [[5jkd\|5jkd]], [[5jkc\|5jkc]], [[5jkb\|5jkb]], [[5jka\|5jka]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jke\|5jke]], [[5jkd\|5jkd]], [[5jkc\|5jkc]], [[5jkb\|5jkb]], [[5jka\|5jka]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jk9 OCA], [http://pdbe.org/5jk9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jk9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jk9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jk9 OCA], [http://pdbe.org/5jk9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jk9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jk9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jk9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/IZUM1_HUMAN IZUM1_HUMAN]] Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.<ref>PMID:15759005</ref>		[[http://www.uniprot.org/uniprot/IZUM1_HUMAN IZUM1_HUMAN]] Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.<ref>PMID:15759005</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fertilization is a fundamental process in sexual reproduction, creating a new individual through the combination of male and female gametes. The IZUMO1 sperm membrane protein and its counterpart oocyte receptor JUNO have been identified as essential factors for sperm-oocyte interaction and fusion. However, the mechanism underlying their specific recognition remains poorly defined. Here, we show the crystal structures of human IZUMO1, JUNO and the IZUMO1-JUNO complex, establishing the structural basis for the IZUMO1-JUNO-mediated sperm-oocyte interaction. IZUMO1 exhibits an elongated rod-shaped structure comprised of a helical bundle IZUMO domain and an immunoglobulin-like domain that are each firmly anchored to an intervening beta-hairpin region through conserved disulfide bonds. The central beta-hairpin region of IZUMO1 provides the main platform for JUNO binding, while the surface located behind the putative JUNO ligand binding pocket is involved in IZUMO1 binding. Structure-based mutagenesis analysis confirms the biological importance of the IZUMO1-JUNO interaction. This structure provides a major step towards elucidating an essential phase of fertilization and it will contribute to the development of new therapeutic interventions for fertility, such as contraceptive agents.
		+
		+	Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization.,Ohto U, Ishida H, Krayukhina E, Uchiyama S, Inoue N, Shimizu T Nature. 2016 Jun 15;534(7608):566-9. doi: 10.1038/nature18596. PMID:27309808<ref>PMID:27309808</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5jk9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:29, 23 June 2016

2016-06-23T23:29:16Z

←Older revision		Revision as of 23:29, 23 June 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5jk9 ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of human IZUMO1==
-		+	<StructureSection load='5jk9' size='340' side='right' caption='[[5jk9]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~Authors~~: ~~Ohto, U~~.~~, Ishida, H~~.~~, Shimizu, T~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5jk9]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JK9 FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of human IZUMO1~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5jke\|5jke]], [[5jkd\|5jkd]], [[5jkc\|5jkc]], [[5jkb\|5jkb]], [[5jka\|5jka]]</td></tr>
-	[[~~Category~~: ~~Shimizu~~, T]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jk9 OCA], [http://pdbe.org/5jk9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jk9 RCSB], [http://www.ebi.ac.uk/pdbsum/5jk9 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IZUM1_HUMAN IZUM1_HUMAN]] Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.<ref>PMID:15759005</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Ishida, H]]		[[Category: Ishida, H]]
	[[Category: Ohto, U]]		[[Category: Ohto, U]]
		+	[[Category: Shimizu, T]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Fertilization]]
		+	[[Category: Izumo1]]
		+	[[Category: Juno]]

OCA at 15:30, 15 May 2016

2016-05-15T15:30:22Z

←Older revision		Revision as of 15:30, 15 May 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5jk9 is ON HOLD	+	The entry 5jk9 is ON HOLD until Paper Publication

	Authors: Ohto, U., Ishida, H., Shimizu, T.		Authors: Ohto, U., Ishida, H., Shimizu, T.

OCA: Protected "5jk9" [edit=sysop:move=sysop]

2016-05-11T02:12:00Z

Protected "5jk9" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:12, 11 May 2016

OCA: New page: '''Unreleased structure''' The entry 5jk9 is ON HOLD Authors: Ohto, U., Ishida, H., Shimizu, T. Description: Crystal structure of human IZUMO1 Category: Unreleased Structures [[Cat...

2016-05-11T02:11:59Z

New page: '''Unreleased structure''' The entry 5jk9 is ON HOLD Authors: Ohto, U., Ishida, H., Shimizu, T. Description: Crystal structure of human IZUMO1 Category: Unreleased Structures [[Cat...

New page

'''Unreleased structure'''

The entry 5jk9 is ON HOLD

Authors: Ohto, U., Ishida, H., Shimizu, T.

Description: Crystal structure of human IZUMO1
[[Category: Unreleased Structures]]
[[Category: Shimizu, T]]
[[Category: Ishida, H]]
[[Category: Ohto, U]]