5jub - Revision history

OCA at 07:24, 1 May 2024

2024-05-01T07:24:50Z

←Older revision		Revision as of 07:24, 1 May 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A1L1QK15_STRTD A0A1L1QK15_STRTD]		[https://www.uniprot.org/uniprot/A0A1L1QK15_STRTD A0A1L1QK15_STRTD]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In Gram-positive bacteria, cell-to-cell communication mainly relies on extracellular signaling peptides, which elicit a response either indirectly, by triggering a two-component phosphorelay, or directly, by binding to cytoplasmic effectors. The latter comprise the RNPP family (Rgg and original regulators Rap, NprR, PrgX and PlcR), whose members regulate important bacterial processes such as sporulation, conjugation, and virulence. RNPP proteins are increasingly considered as interesting targets for the development of new antibacterial agents. These proteins are characterized by a TPR-type peptide-binding domain, and except for Rap proteins, also contain an N-terminal HTH-type DNA-binding domain and display a transcriptional activity. Here, we elucidate the structure-function relationship of the transcription factor ComR, a new member of the RNPP family, which positively controls competence for natural DNA transformation in streptococci. ComR is directly activated by the binding of its associated pheromone XIP, the mature form of the comX/sigX-inducing-peptide ComS. The crystal structure analysis of ComR from Streptococcus thermophilus combined with a mutational analysis and in vivo assays allows us to propose an original molecular mechanism of the ComR regulation mode. XIP-binding induces release of the sequestered HTH domain and ComR dimerization to allow DNA binding. Importantly, we bring evidence that this activation mechanism is conserved and specific to ComR orthologues, demonstrating that ComR is not an Rgg protein as initially proposed, but instead constitutes a new member of the RNPP family. In addition, identification of XIP and ComR residues important for competence activation constitutes a crucial step towards the design of antagonistic strategies to control gene exchanges among streptococci.
-
-	Structural Insights into Streptococcal Competence Regulation by the Cell-to-Cell Communication System ComRS.,Talagas A, Fontaine L, Ledesma-Garca L, Mignolet J, Li de la Sierra-Gallay I, Lazar N, Aumont-Nicaise M, Federle MJ, Prehna G, Hols P, Nessler S PLoS Pathog. 2016 Dec 1;12(12):e1005980. doi: 10.1371/journal.ppat.1005980., eCollection 2016 Dec. PMID:27907189<ref>PMID:27907189</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5jub" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Transcriptional activator 3D structures\|Transcriptional activator 3D structures]]		*[[Transcriptional activator 3D structures\|Transcriptional activator 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 19:06, 20 September 2023

2023-09-20T19:06:59Z

←Older revision		Revision as of 19:06, 20 September 2023
Line 3:		Line 3:
	<StructureSection load='5jub' size='340' side='right'caption='[[5jub]], [[Resolution\|resolution]] 2.57Å' scene=''>		<StructureSection load='5jub' size='340' side='right'caption='[[5jub]], [[Resolution\|resolution]] 2.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jub]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strtd Strtd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JUB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5JUB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jub]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_thermophilus_LMD-9 Streptococcus thermophilus LMD-9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JUB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5jub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jub OCA], [~~http~~://pdbe.org/5jub PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5jub RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5jub PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5jub ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.57Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jub OCA], [https://pdbe.org/5jub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jub RCSB], [https://www.ebi.ac.uk/pdbsum/5jub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jub ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1L1QK15_STRTD A0A1L1QK15_STRTD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5jub" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5jub" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Transcriptional activator 3D structures\|Transcriptional activator 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 20:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Strtd~~]]	+	[[Category: Streptococcus thermophilus LMD-9]]
-	[[Category: Fontaine, L]]	+	[[Category: Fontaine L]]
-	[[Category: Hols, P]]	+	[[Category: Hols P]]
-	[[Category: Ledesma-Garcia, L]]	+	[[Category: Ledesma-Garcia L]]
-	[[Category: ~~Nessler, S]]~~	+	[[Category: Li de la Sierra-Gallay I]]
-	~~[[Category:~~ Sierra-Gallay, I ~~Li de la~~]]	+	[[Category: Nessler S]]
-	[[Category: ~~Talagas, A~~]]	+	[[Category: Talagas A]]
-	[[Category: ~~Competence regulator]]~~	+
-	~~[[Category: Hth domain]]~~	+
-	~~[[Category: Quorum-sensor]]~~	+
-	~~[[Category: Rnpp]]~~	+
-	~~[[Category: Tpr domain]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcriptional activator~~]]	+

OCA at 11:40, 17 June 2020

2020-06-17T11:40:08Z

←Older revision		Revision as of 11:40, 17 June 2020
Line 1:		Line 1:

	==Crystal structure of ComR from S.thermophilus in complex with DNA and its signalling peptide ComS.==		==Crystal structure of ComR from S.thermophilus in complex with DNA and its signalling peptide ComS.==
-	<StructureSection load='5jub' size='340' side='right' caption='[[5jub]], [[Resolution\|resolution]] 2.57Å' scene=''>	+	<StructureSection load='5jub' size='340' side='right'caption='[[5jub]], [[Resolution\|resolution]] 2.57Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5jub]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JUB OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5JUB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5jub]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Strtd Strtd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JUB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JUB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5jub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jub OCA], [http://pdbe.org/5jub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jub RCSB], [http://www.ebi.ac.uk/pdbsum/5jub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jub ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jub OCA], [http://pdbe.org/5jub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jub RCSB], [http://www.ebi.ac.uk/pdbsum/5jub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jub ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Strtd]]
	[[Category: Fontaine, L]]		[[Category: Fontaine, L]]
	[[Category: Hols, P]]		[[Category: Hols, P]]

OCA at 11:40, 15 December 2016

2016-12-15T11:40:27Z

←Older revision		Revision as of 11:40, 15 December 2016
Line 6:		Line 6:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jub OCA], [http://pdbe.org/5jub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jub RCSB], [http://www.ebi.ac.uk/pdbsum/5jub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jub ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jub OCA], [http://pdbe.org/5jub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jub RCSB], [http://www.ebi.ac.uk/pdbsum/5jub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jub ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In Gram-positive bacteria, cell-to-cell communication mainly relies on extracellular signaling peptides, which elicit a response either indirectly, by triggering a two-component phosphorelay, or directly, by binding to cytoplasmic effectors. The latter comprise the RNPP family (Rgg and original regulators Rap, NprR, PrgX and PlcR), whose members regulate important bacterial processes such as sporulation, conjugation, and virulence. RNPP proteins are increasingly considered as interesting targets for the development of new antibacterial agents. These proteins are characterized by a TPR-type peptide-binding domain, and except for Rap proteins, also contain an N-terminal HTH-type DNA-binding domain and display a transcriptional activity. Here, we elucidate the structure-function relationship of the transcription factor ComR, a new member of the RNPP family, which positively controls competence for natural DNA transformation in streptococci. ComR is directly activated by the binding of its associated pheromone XIP, the mature form of the comX/sigX-inducing-peptide ComS. The crystal structure analysis of ComR from Streptococcus thermophilus combined with a mutational analysis and in vivo assays allows us to propose an original molecular mechanism of the ComR regulation mode. XIP-binding induces release of the sequestered HTH domain and ComR dimerization to allow DNA binding. Importantly, we bring evidence that this activation mechanism is conserved and specific to ComR orthologues, demonstrating that ComR is not an Rgg protein as initially proposed, but instead constitutes a new member of the RNPP family. In addition, identification of XIP and ComR residues important for competence activation constitutes a crucial step towards the design of antagonistic strategies to control gene exchanges among streptococci.
		+
		+	Structural Insights into Streptococcal Competence Regulation by the Cell-to-Cell Communication System ComRS.,Talagas A, Fontaine L, Ledesma-Garca L, Mignolet J, Li de la Sierra-Gallay I, Lazar N, Aumont-Nicaise M, Federle MJ, Prehna G, Hols P, Nessler S PLoS Pathog. 2016 Dec 1;12(12):e1005980. doi: 10.1371/journal.ppat.1005980., eCollection 2016 Dec. PMID:27907189<ref>PMID:27907189</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5jub" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 18:46, 26 October 2016

2016-10-26T18:46:33Z

←Older revision		Revision as of 18:46, 26 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5jub is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of ComR from S.thermophilus in complex with DNA and its signalling peptide ComS.==
-		+	<StructureSection load='5jub' size='340' side='right' caption='[[5jub]], [[Resolution\|resolution]] 2.57Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5jub]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JUB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JUB FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jub OCA], [http://pdbe.org/5jub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jub RCSB], [http://www.ebi.ac.uk/pdbsum/5jub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jub ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fontaine, L]]
		+	[[Category: Hols, P]]
		+	[[Category: Ledesma-Garcia, L]]
		+	[[Category: Nessler, S]]
		+	[[Category: Sierra-Gallay, I Li de la]]
		+	[[Category: Talagas, A]]
		+	[[Category: Competence regulator]]
		+	[[Category: Hth domain]]
		+	[[Category: Quorum-sensor]]
		+	[[Category: Rnpp]]
		+	[[Category: Tpr domain]]
		+	[[Category: Transcription]]
		+	[[Category: Transcriptional activator]]

OCA at 23:42, 12 July 2016

2016-07-12T23:42:03Z

←Older revision		Revision as of 23:42, 12 July 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5jub is ON HOLD	+	The entry 5jub is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5jub" [edit=sysop:move=sysop]

2016-06-01T13:50:44Z

Protected "5jub" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:50, 1 June 2016

OCA: New page: '''Unreleased structure''' The entry 5jub is ON HOLD Authors: Description: Category: Unreleased Structures

2016-06-01T13:50:43Z

New page: '''Unreleased structure''' The entry 5jub is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5jub is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]